BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0021.p (226 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAB62314.1| rhamnosidase A [Bacillus sp. GL1] 233 2e-60 ref|NP_978576.1| rhamnosidase A [Bacillus cereus ATCC 10987] >gi... 217 2e-55 ref|ZP_00185631.1| hypothetical protein Rxyl364701 [Rubrobacter ... 202 6e-51 ref|NP_822003.1| putative rhamnosidase [Streptomyces avermitilis... 173 3e-42 ref|NP_811436.1| alpha-rhamnosidase [Bacteroides thetaiotaomicro... 166 5e-40 >dbj|BAB62314.1| rhamnosidase A [Bacillus sp. GL1] Length = 886 Score = 233 bits (595), Expect = 2e-60, Method: Compositional matrix adjust. Identities = 113/223 (50%), Positives = 147/223 (65%), Gaps = 1/223 (0%) Query: 1 MFGLVEGPAKERTTDKLLKLLEESGHHLTTGFVGTPYLNLVLSEAGHTDAAYKLLLQTDY 60 MF L+E +++ D+L KL+EESG LTTGFVGTPYL VL+ G D AYKLL + +Y Sbjct: 664 MFDLLEEGTRQQAADRLAKLIEESGTQLTTGFVGTPYLCHVLTRFGRADLAYKLLERREY 723 Query: 61 PSWLYQVTQGATTIWEHWDGIKEDGSFWSKDMNSFNHYAYGAIGDFLYRHVAGIELDEAY 120 PSWLY V +GATTIWEHWDGIK DGSFWS DMNS+NHYAYGAIGD+L+ VAGI+ + + Sbjct: 724 PSWLYPVVKGATTIWEHWDGIKPDGSFWSDDMNSYNHYAYGAIGDWLFGSVAGIDTEASA 783 Query: 121 PGYKAFVIKPQPGGDLTWTAAALETMYGRIRSEWNITAD-QLEMSVSVPPNTSATLYLPK 179 PGYK ++P PGG L + A LE+ YG IRS W D ++ VP N +A + LP Sbjct: 784 PGYKRIRVRPVPGGSLKFAEATLESPYGEIRSAWRRRPDGGIDYEFEVPANAAANVLLPD 843 Query: 180 ADLPRVLEGDVPLEHAAGVHSTEQLEHEVKVILGSGDYKFSYK 222 A +E P++ A G+ + + ++GSG Y+F+ K Sbjct: 844 ASKANAMESQQPIDAAEGISVVTETAEGLAFVVGSGTYRFTVK 886 >ref|NP_978576.1| rhamnosidase A [Bacillus cereus ATCC 10987] gb|AAS41184.1| rhamnosidase A [Bacillus cereus ATCC 10987] Length = 860 Score = 217 bits (552), Expect = 2e-55, Method: Compositional matrix adjust. Identities = 107/226 (47%), Positives = 145/226 (64%), Gaps = 20/226 (8%) Query: 1 MFGLVEGPAKERTTDKLLKLLEESGHHLTTGFVGTPYLNLVLSEAGHTDAAYKLLLQTDY 60 +F LV+ KE+ ++L++LL+E+ +HLTTGF+GTPYLNL+LS+ D A KLL DY Sbjct: 654 LFELVKDNMKEKVFNRLIELLKENKNHLTTGFIGTPYLNLILSKFNRHDLACKLLFHEDY 713 Query: 61 PSWLYQVTQGATTIWEHWDGIKEDGSFWSKDMNSFNHYAYGAIGDFLYRHVAGIELDEAY 120 PSWLYQV QGATTIWEHWDG+KEDG+ W+ MNS+NHYAYG+I +++YR++ G+E+DE Sbjct: 714 PSWLYQVKQGATTIWEHWDGVKEDGALWNDSMNSYNHYAYGSIVEWIYRYIIGLEVDETK 773 Query: 121 PGYKAFVIKPQPGGDLTWTAAALETMYGRIRSEWNITADQLEMSVSVPPNTSATLYLPKA 180 P YK F I+P +L W ET YG I+ +W + Q ++V VP NTSATL + + Sbjct: 774 PAYKHFYIQPHFNSNLDWVCIKRETAYGEIKIDWRVEHGQAVLNVDVPTNTSATLRIVED 833 Query: 181 DLPRVLEGDVPLEHAAGVHSTEQLEHEVKVILGSGDYKFSYKLGAQ 226 D D+P E+K LGSG Y+F + Q Sbjct: 834 DWR---SSDMP---------------EMK--LGSGAYEFRFCKNIQ 859 >ref|ZP_00185631.1| hypothetical protein Rxyl364701 [Rubrobacter xylanophilus DSM 9941] Length = 433 Score = 202 bits (513), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 104/208 (50%), Positives = 136/208 (65%), Gaps = 7/208 (3%) Query: 16 KLLKLLEESGHHLTTGFVGTPYLNLVLSEAGHTDAAYKLLLQTDYPSWLYQVTQGATTIW 75 +L++ +E G HL+TGF+GTP+L VL+EAG D AY+LL Q +PSWLY+V +GATT W Sbjct: 231 RLVEHIEARGWHLSTGFLGTPHLLPVLTEAGRADVAYRLLNQRTFPSWLYEVERGATTTW 290 Query: 76 EHWDGIKEDGSFWSKDMNSFNHYAYGAIGDFLYRHVAGIELDEAYPGYKAFVIKPQPGGD 135 E WD IK DGSF MNSFNHYAYGA+GD+LYR+VAGI +E PGY+ +I+P+PGG Sbjct: 291 ERWDSIKPDGSFNDPAMNSFNHYAYGAVGDWLYRYVAGISPEE--PGYRRILIRPRPGGG 348 Query: 136 LTWTAAALETMYGRIRSEWNITADQLEMSVSVPPNTSATLYLPKADLPRVLEGDVPLEHA 195 L LE+ YGRI SEW + +L M VS+PPNT A + +P D R + P+ A Sbjct: 349 LDRARGELESPYGRILSEWRLRGGKLRMRVSIPPNTRAGVCVPARDA-REVRVSPPVPGA 407 Query: 196 AGVHSTEQLEHEVKVILGSGDYKFSYKL 223 + Q + LGSG Y+F +L Sbjct: 408 ----NPSQRDGCAVFELGSGTYEFRSRL 431 >ref|NP_822003.1| putative rhamnosidase [Streptomyces avermitilis MA-4680] dbj|BAC68538.1| putative rhamnosidase [Streptomyces avermitilis MA-4680] Length = 1030 Score = 173 bits (438), Expect = 3e-42, Method: Compositional matrix adjust. Identities = 87/220 (39%), Positives = 135/220 (61%), Gaps = 12/220 (5%) Query: 2 FGLVEGPAKERTTDKLLKLLEESGHHLTTGFVGTPYLNLVLSEAGHTDAAYKLLLQTDYP 61 LV ++ D+L+ L+E HL+TGF+GTP L VL++ GHTD AY+LL Q +P Sbjct: 821 MNLVPDALRKAAADRLVALIEAKDWHLSTGFLGTPRLLPVLTDTGHTDVAYRLLHQRTFP 880 Query: 62 SWLYQVTQGATTIWEHWDGIKEDGSFWSKDMNSFNHYAYGAIGDFLYRHVAGIELDEAYP 121 SW Y + +G+TT+WE WD I+ DG F + +MNSFNHYAYG++G+++Y ++AGI A Sbjct: 881 SWGYPIDKGSTTMWERWDSIQPDGGFQTPEMNSFNHYAYGSVGEWMYANIAGIAPGRA-- 938 Query: 122 GYKAFVIKPQPGGDLTWTAAALETMYGRIRSEWNITADQLEMSVSVPPNTSATLYLPKAD 181 GY+ VI+P+PGG++T A +++G + + W + ++ SVPPNT+A +++P Sbjct: 939 GYRQVVIRPRPGGEVTSARATFASLHGPVSTRWQQRSGGFVLTCSVPPNTTAEVWIPADH 998 Query: 182 LPRVLEGDVPLEHAAGVHSTEQLEHEVKVI-LGSGDYKFS 220 RV +H G + E V +GSG ++F+ Sbjct: 999 PDRV-------QHTHGTFV--RAEDGCAVFEVGSGSHRFT 1029 >ref|NP_811436.1| alpha-rhamnosidase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO77630.1| alpha-rhamnosidase [Bacteroides thetaiotaomicron VPI-5482] Length = 1152 Score = 166 bits (419), Expect = 5e-40, Method: Compositional matrix adjust. Identities = 89/226 (39%), Positives = 133/226 (58%), Gaps = 13/226 (5%) Query: 9 AKERTTDKLLKLLEESGHHLTTGFVGTPYLNLVLSEAGHTDAAYKLLLQTDYPSWLYQVT 68 A+++ + L++ + +G+ L+TGFVGT LN LS+ G ++ AY LLLQ + PSWLY + Sbjct: 924 ARKKGVETLVRKIAGNGNRLSTGFVGTAILNQTLSQFGESNTAYDLLLQRNNPSWLYSID 983 Query: 69 QGATTIWEHWDGIKEDGSFWSKDMNSFNHYAYGAIGDFLYRHVAGIELDEAYPGYKAFVI 128 QGATTIWE WD ++ F MNSFNHY+YGA+ +++YR + GI++DE PG+K V+ Sbjct: 984 QGATTIWERWDSYTKEKGFGPVSMNSFNHYSYGAVSEWMYRTMGGIDIDETRPGFKYIVL 1043 Query: 129 KPQPGG---------DLTWTAAALETMYGRIRSEWNITAD-QLEMSVSVPPNTSATLYLP 178 +P P + W A+ + YG I+S W D + V++P NT+ATL+L Sbjct: 1044 QPVPDNRPEVAAGQERIDWVNASFPSCYGDIKSSWKKENDGTVSYQVTIPANTTATLHLL 1103 Query: 179 KADLPRVLE--GDVPLEHAAGVHSTEQLEHEVKVILGSGDYKFSYK 222 L V+E G ++ A GV S + + + L SG Y+F K Sbjct: 1104 LPTLDYVVEESGKAAVK-AEGVSSVTFMNGKAVLELQSGTYQFVVK 1148 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.414 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 373,724,214 Number of extensions: 15789822 Number of successful extensions: 35134 Number of sequences better than 1.0e-05: 29 Number of HSP's gapped: 35105 Number of HSP's successfully gapped: 29 Length of query: 226 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 105 Effective length of database: 428,710,599 Effective search space: 45014612895 Effective search space used: 45014612895 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 124 (52.4 bits)