BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0019.p (301 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00180981.1| hypothetical protein Mther104601 [Moorella th... 120 3e-26 ref|ZP_00078770.1| COG0500: SAM-dependent methyltransferases [Me... 86 7e-16 ref|NP_634483.1| methyltransferase [Methanosarcina mazei Goe1] >... 86 1e-15 ref|NP_615800.1| conserved hypothetical protein [Methanosarcina ... 80 7e-14 ref|NP_616296.1| conserved hypothetical protein [Methanosarcina ... 69 2e-10 >ref|ZP_00180981.1| hypothetical protein Mther104601 [Moorella thermoacetica ATCC 39073] Length = 289 Score = 120 bits (302), Expect = 3e-26, Method: Compositional matrix adjust. Identities = 76/267 (28%), Positives = 130/267 (48%), Gaps = 2/267 (0%) Query: 5 LFNADVWEKAWKEDPAAMGNRFKQSGMDMARAFDHKAKSFNEEVFXXXXXXXXXXXXXWL 64 L AD WE+AW+E+ ++ D + ++ +A F + WL Sbjct: 6 LERADFWEEAWREEHRNSLYGRRRPERDGSAYWNRRAGHFARQTGSEEGKQRVAGILHWL 65 Query: 65 EGQGVDFEGLSVLDVGAASGGFTVPFADRGAKVTAVEPNHPLAGLFIENTARFGPGQVEL 124 G + L VLD+G +G + +P A R +V A++P + + E A G VE Sbjct: 66 GHHGGLEKDLEVLDIGCGTGNYALPLARRARRVVALDPAAEMLAILKEKAAAEGINNVEP 125 Query: 125 VHDLFEHVDLDAKGWKGAFDLVFASMTPVVVDWESVESVLSCARKYCYISLNAGGREFSL 184 + +E VDL+ +GW+ AFDLV A M+P + D +++ +++ +R C+++ + +E S Sbjct: 126 IQMAWEEVDLEKQGWREAFDLVLALMSPGIKDAATLQKMMAASRGACFLAGHV-RQEISG 184 Query: 185 LNEVLPLLNGQTLPAKSSDMAYLTHLLYLKGYSFESLITREMKTTELSSGEAIEETLMLL 244 E+ L G +P +D+ Y+ HLLY GY+ + ++ T EL +AIEE + Sbjct: 185 RRELWQELIGGEMPPIPADVLYIFHLLYAWGYNPSLELEHKVSTRELEPDQAIEEMEIFF 244 Query: 245 KHHKLPADEAPRRTVTEYVHRTYPDGK 271 H L A RR + +V +G+ Sbjct: 245 YPH-LELTPAVRRAIINHVQANTVNGR 270 >ref|ZP_00078770.1| COG0500: SAM-dependent methyltransferases [Methanosarcina barkeri] Length = 286 Score = 86.3 bits (212), Expect = 7e-16, Method: Compositional matrix adjust. Identities = 57/207 (27%), Positives = 99/207 (47%), Gaps = 2/207 (0%) Query: 64 LEGQGVDFEGLSVLDVGAASGGFTVPFADRGAKVTAVEPNHPLAGLFIENTARFGPGQVE 123 LE G + EG LD+G G T+P + GA+VTA++ + + ++++ + V+ Sbjct: 62 LEEAGFNPEGSRALDIGCGPGTLTLPLSKLGAEVTALDISSGMLDR-LKDSVKKESLPVD 120 Query: 124 LVHDLFEHVDLDAKGWKGAFDLVFASMTPVVVDWESVESVLSCARKYCYISLNAGGREFS 183 +V + D+D G++ FDLV ASMTP V D ES + +++C++ CY S E Sbjct: 121 IVECSWWTADIDELGFRNEFDLVIASMTPGVKDIESFDKMMACSKNLCYYSNFLRREEDR 180 Query: 184 LLNEVLPLLNGQTLPAKSSDMAYLTHLLYLKGYSFESLITREMKTTELSSGEAIEETLML 243 ++ + G+ + + Y LYL GY I EL+ E E+T+ Sbjct: 181 AYRDIRSSILGEKSENNMNGIIYPFMYLYLSGYKPSLRINHSEWKDELNWKETAEQTIGF 240 Query: 244 LKHHKLPADEAPRRTVTEYVHRTYPDG 270 + + D+ ++ + +Y PDG Sbjct: 241 IGRDR-DFDDETKQKIRDYYQNASPDG 266 >ref|NP_634483.1| methyltransferase [Methanosarcina mazei Goe1] gb|AAM32155.1| methyltransferase [Methanosarcina mazei Goe1] Length = 287 Score = 85.9 bits (211), Expect = 1e-15, Method: Compositional matrix adjust. Identities = 57/238 (23%), Positives = 110/238 (46%), Gaps = 2/238 (0%) Query: 33 MARAFDHKAKSFNEEVFXXXXXXXXXXXXXWLEGQGVDFEGLSVLDVGAASGGFTVPFAD 92 MA ++ +++++ + +LE G D EG VLD+G G ++P + Sbjct: 32 MADFWNKRSENYANNIGKDNRKKRTDEILEFLEESGFDPEGSRVLDIGCGPGTLSLPLSK 91 Query: 93 RGAKVTAVEPNHPLAGLFIENTARFGPGQVELVHDLFEHVDLDAKGWKGAFDLVFASMTP 152 GA+VTA++ + + +++T + V+++ + D+D+ G++ FDLV ASMTP Sbjct: 92 LGAEVTALDISSGMLKR-LKDTVKEESLPVDVIETSWWTADIDSLGFRDRFDLVIASMTP 150 Query: 153 VVVDWESVESVLSCARKYCYISLNAGGREFSLLNEVLPLLNGQTLPAKSSDMAYLTHLLY 212 + D E+ + +++C++ +CY S E E+ + G+ + M + LY Sbjct: 151 AIKDIENFDKMMACSKNFCYYSNFLRRGEDRAYREIRSSILGENSANNMNGMTFPFMYLY 210 Query: 213 LKGYSFESLITREMKTTELSSGEAIEETLMLLKHHKLPADEAPRRTVTEYVHRTYPDG 270 L GY I E + EA ++ + + + DE + + +Y DG Sbjct: 211 LSGYMPSVRINHSEWKEETNWREAADQAIEFIGRGR-DFDEETKEKIRDYYQNASQDG 267 >ref|NP_615800.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM04280.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 302 Score = 79.7 bits (195), Expect = 7e-14, Method: Compositional matrix adjust. Identities = 56/243 (23%), Positives = 110/243 (45%), Gaps = 2/243 (0%) Query: 33 MARAFDHKAKSFNEEVFXXXXXXXXXXXXXWLEGQGVDFEGLSVLDVGAASGGFTVPFAD 92 MA ++ +++++ + +LE G + EG VLD+G G ++P + Sbjct: 47 MADFWNKRSENYANNIEKDKRKKRTDEILEFLEEAGFNPEGSRVLDIGCGPGTLSLPLSR 106 Query: 93 RGAKVTAVEPNHPLAGLFIENTARFGPGQVELVHDLFEHVDLDAKGWKGAFDLVFASMTP 152 GA+VTA++ + + +++ + V+++ + D+D G++ FDLV ASMTP Sbjct: 107 LGAEVTALDISSGMLDR-LKDAVKKESLPVDVIECSWWTADIDELGFRNKFDLVIASMTP 165 Query: 153 VVVDWESVESVLSCARKYCYISLNAGGREFSLLNEVLPLLNGQTLPAKSSDMAYLTHLLY 212 + D E+ +++C++ CY S E ++ + G+ + + Y LY Sbjct: 166 GIRDVENFNMMMACSKNLCYYSNFLRRVEDKAYRDIRSSILGERSENNMNGIIYPFMYLY 225 Query: 213 LKGYSFESLITREMKTTELSSGEAIEETLMLLKHHKLPADEAPRRTVTEYVHRTYPDGKV 272 L GYS I E + EA E+ + + + D+ + + +Y PDG Sbjct: 226 LSGYSPSLRINHSEWKDETNWQEAAEQAIGFIGRGR-DFDDETKEKIMDYYQNASPDGTY 284 Query: 273 QVQ 275 + + Sbjct: 285 RSE 287 >ref|NP_616296.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM04776.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 281 Score = 68.6 bits (166), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 46/143 (32%), Positives = 77/143 (53%), Gaps = 6/143 (4%) Query: 76 VLDVGAASGGFTVPFADRGAKVTAVEPNHPLAGLFIENTARFGPGQVELVHDLFEHVDLD 135 VLD+GA G +P + R A VTAVEP+ + + +N +G ++ VH +E VD++ Sbjct: 69 VLDIGAGPGTLAIPISQRVAHVTAVEPSEGMISVMQDNIKEYGIENIDPVHKDWESVDVE 128 Query: 136 AKGWKGAFDLVFASMTPVVVDWE-SVESVLSCARKYCYISLNAGGREFSLLNEVL-PLLN 193 + +D+VFAS + + D SV+ ++ + KY Y+ AG + + + L P L+ Sbjct: 129 SDL-SAPYDVVFASYSLGMKDIRTSVQKMVDASSKYVYLYWFAGETSWDMHSRKLWPFLH 187 Query: 194 G-QTLPAKSSDMAYLTHLLYLKG 215 G + PA D+ Y ++LY G Sbjct: 188 GSEYQPAPKCDVLY--NVLYDMG 208 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.135 0.403 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 434,905,318 Number of extensions: 17208506 Number of successful extensions: 42130 Number of sequences better than 1.0e-05: 10 Number of HSP's gapped: 42129 Number of HSP's successfully gapped: 10 Length of query: 301 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 176 Effective length of database: 417,850,203 Effective search space: 73541635728 Effective search space used: 73541635728 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)