BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0010.p (491 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00036275.1| COG2721: Altronate dehydratase [Enterococcus ... 708 0.0 ref|NP_389121.1| similar to altronate hydrolase [Bacillus subtil... 603 e-171 ref|NP_347333.1| Putative altronate dehydratase [Clostridium ace... 587 e-166 ref|NP_809399.1| altronate hydrolase [Bacteroides thetaiotaomicr... 575 e-163 ref|NP_755720.1| Altronate hydrolase [Escherichia coli CFT073] >... 560 e-158 >ref|ZP_00036275.1| COG2721: Altronate dehydratase [Enterococcus faecium] Length = 499 Score = 708 bits (1828), Expect = 0.0, Method: Compositional matrix adjust. Identities = 335/491 (68%), Positives = 392/491 (79%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 MK+N RD+V VAL PI L+ + ++T +DIPQGHKIAL GD + KYGYPIG Sbjct: 9 MKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIPQGHKIALGDLKAGDNVIKYGYPIG 68 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 H ++ AG W+HTHN+KTNLSGE +Y Y D+HP Y TFQGY R NGKVGIRND Sbjct: 69 HVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVHPSHYLFENRTFQGYLRKNGKVGIRND 128 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 LF++PTVGCVNGIAE ++++FK +HPDLG FD+ T+LKHPYGCSQLG DH+ TR IL DA Sbjct: 129 LFLVPTVGCVNGIAELIVKQFKEKHPDLGQFDHITILKHPYGCSQLGKDHQNTREILADA 188 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 VNHPNAGGVLVFGLGCENN V EF+ +LG+YD RVKFLVAQ+V DE+ Sbjct: 189 VNHPNAGGVLVFGLGCENNTVPEFKKILGEYDEERVKFLVAQDVYDEIEQGVALLEELLT 248 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 +ND R VPLS+LN+GLKCGGSDG SGITANPLLGAFSD++I+QGG+++LTEVPEMFG Sbjct: 249 AAENDQRISVPLSKLNVGLKCGGSDGLSGITANPLLGAFSDYLIAQGGSTILTEVPEMFG 308 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 AE++LMARAEN EVF+ IV LIN+FKQYFLSYGEPVYENPSPGNKAGGI+TLEDKSLGCT Sbjct: 309 AEQVLMARAENEEVFDSIVDLINDFKQYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCT 368 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 QK+GSSPVVDVL YG K+R+ GLSLLQAPGN CQLVLFTTGRGTPFGS+ Sbjct: 369 QKSGSSPVVDVLQYGEKIRKPGLSLLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSY 428 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLERPMADVLEEFIAYIIDVASGQKTRNEQNEVR 480 VPTVKV+TN LF +KGHWMDFNAG LLE+PM +LE F+ II VASG++T+NEQN+VR Sbjct: 429 VPTVKVSTNTTLFERKGHWMDFNAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVR 488 Query: 481 ELAIFKTGVTL 491 E+AIFK GVTL Sbjct: 489 EIAIFKNGVTL 499 >ref|NP_389121.1| similar to altronate hydrolase [Bacillus subtilis] sp|O34673|UXAA_BACSU Altronate hydrolase (Altronic acid hydratase) pir||D69853 altronate hydrolase homolog yjmJ - Bacillus subtilis gb|AAC46335.1| hypothetical altronate dehydratase [Bacillus subtilis] emb|CAB13096.1| altronate hydrolase [Bacillus subtilis subsp. subtilis str. 168] Length = 497 Score = 603 bits (1554), Expect = e-171, Method: Compositional matrix adjust. Identities = 299/493 (60%), Positives = 356/493 (72%), Gaps = 2/493 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 +K++ +D V +ALR I GE L G+S+ DI +GHKIAL S E D I KYG+PIG Sbjct: 5 IKIHKQDNVLLALRYIQKGERLHAYGVSIEVKDDIKRGHKIALQSIKENDSIVKYGFPIG 64 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 HA ++I G+ IH HN KTNLS + Y Y P Y TF+G+RR NG G+RN+ Sbjct: 65 HASQDISIGEHIHVHNTKTNLSDIQLYSYTPRFDENPYSNENRTFKGFRRENGDAGVRNE 124 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 L+I+PTVGCVNGIAE+MLQ F E D+ FDN VLKH YGCSQLGDDH T+ ILL+A Sbjct: 125 LWIVPTVGCVNGIAEKMLQRFVRETGDIAPFDNVLVLKHQYGCSQLGDDHENTKQILLNA 184 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 + HPNAGGVLV GLGCENN ++ + L D + RVKFL +Q V DE+ Sbjct: 185 IRHPNAGGVLVLGLGCENNELARMKEALQDVNLKRVKFLESQSVTDEMEAGVALLKEIHE 244 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 K D RE +PLSEL IGLKCGGSDGFSGITANPLLG FSD++I+QGG++VLTEVPEMFG Sbjct: 245 AAKGDKREDIPLSELKIGLKCGGSDGFSGITANPLLGRFSDYLIAQGGSTVLTEVPEMFG 304 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 AE +LM RA N EVF IV LIN+FKQYF+ + +PVYENPSPGNKAGGISTLEDKSLGCT Sbjct: 305 AETILMQRAANEEVFHKIVDLINDFKQYFIKHDQPVYENPSPGNKAGGISTLEDKSLGCT 364 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 QKAG SPV DVL YG L+ KGL+LL APGN CQ+VLFTTGRGTPFG+F Sbjct: 365 QKAGISPVTDVLKYGEVLKTKGLTLLSAPGNDLIASSALAAAGCQIVLFTTGRGTPFGTF 424 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLERPMAD--VLEEFIAYIIDVASGQKTRNEQNE 478 VPTVKVATN +L+ K HW+DFNAG L E + + VL EFI Y+I+VASGQ +E+N+ Sbjct: 425 VPTVKVATNTELYEAKPHWIDFNAGLLAEDDVHEEYVLREFIHYMIEVASGQLVNHEKND 484 Query: 479 VRELAIFKTGVTL 491 +ELAIFK+GVTL Sbjct: 485 FKELAIFKSGVTL 497 >ref|NP_347333.1| Putative altronate dehydratase [Clostridium acetobutylicum] pir||F96985 probable altronate dehydratase [imported] - Clostridium acetobutylicum gb|AAK78673.1| Putative altronate dehydratase [Clostridium acetobutylicum] Length = 492 Score = 587 bits (1514), Expect = e-166, Method: Compositional matrix adjust. Identities = 281/492 (57%), Positives = 357/492 (72%), Gaps = 5/492 (1%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 +K+N +D V VAL + G+V+ +G +TA + + +GHKIA+T + I KYG+PIG Sbjct: 5 IKINEKDNVVVALNDLNKGDVIEIDGKVITAEEPVKKGHKIAITDIQKNSNIYKYGFPIG 64 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 HA+EEI+ G W+HTHNIKTNL G ++YEY + LTF+GYRR +G VGIRN+ Sbjct: 65 HALEEIKKGQWVHTHNIKTNLDGIKDYEYNKQTFENPFKNENLTFKGYRREDGTVGIRNE 124 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 L+I+PTVGCVNG A+ + + FK+E F + V KH +GCSQLGDDH TR+IL + Sbjct: 125 LWIVPTVGCVNGTADLIAERFKSETE----FKDVHVFKHNFGCSQLGDDHNNTRTILGNI 180 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 V HPNAGGVLV GLGCENN + F+ L Y+ RV+FL+AQ+V DE+ Sbjct: 181 VKHPNAGGVLVLGLGCENNTMESFKESLHSYNKERVRFLIAQDVEDEISSGCELLKELYE 240 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 + D RE V +SEL IGLKCG SDGFSGITANPLLG SDF+I+QGGT++LTEVPEMFG Sbjct: 241 KIQKDEREEVSISELKIGLKCGASDGFSGITANPLLGKLSDFLIAQGGTTILTEVPEMFG 300 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 AE +LM RA++ +VF V+LIN+FK+YF+SY +PVYENPSPGNKAGGI+TLEDKSLGCT Sbjct: 301 AETILMNRAKDEKVFAKTVNLINDFKKYFMSYNQPVYENPSPGNKAGGITTLEDKSLGCT 360 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 QK+GSS VV VL YG L KGL+LL APGN C +VLFTTGRGTPFG+F Sbjct: 361 QKSGSSEVVGVLKYGETLENKGLNLLSAPGNDLVASTALASAGCHMVLFTTGRGTPFGTF 420 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 VPTVK++TN+D++ KK +W+DFNAG LLE + M VL+EFI Y++ VA+G NE+N + Sbjct: 421 VPTVKISTNSDIYNKKKNWIDFNAGALLENQSMDQVLKEFINYLLGVANGNMANNEKNNI 480 Query: 480 RELAIFKTGVTL 491 RE++IFK GVTL Sbjct: 481 REISIFKNGVTL 492 >ref|NP_809399.1| altronate hydrolase [Bacteroides thetaiotaomicron VPI-5482] gb|AAO75593.1| altronate hydrolase [Bacteroides thetaiotaomicron VPI-5482] Length = 496 Score = 575 bits (1483), Expect = e-163, Method: Compositional matrix adjust. Identities = 287/492 (58%), Positives = 348/492 (70%), Gaps = 2/492 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 +++NP D VAVA+ + +GE LS +G+ +T +DIP GHK AL FAEG+ + KYGYPIG Sbjct: 6 LRINPADNVAVAIVNLPAGEHLSVDGIEITLNEDIPAGHKFALKDFAEGENVIKYGYPIG 65 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 HA + GDW++ NIKTNL+G EY Y P + P + LTF+GYRR NG VG+RN+ Sbjct: 66 HARMAKKQGDWMNETNIKTNLAGLLEYTYHPTQVTLDIPHKNLTFKGYRRKNGDVGVRNE 125 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 ++IIPTVGCVNGI Q+ + + E + G D H YGCSQLGDDH T+ IL D Sbjct: 126 IWIIPTVGCVNGIIGQLAEGLRRE-TEGKGVDAIVAFPHNYGCSQLGDDHENTKKILRDM 184 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 V HPNAG VL+ GLGCENN FR LGDYD RVKF+V Q+VGDE Sbjct: 185 VLHPNAGAVLIVGLGCENNQPDVFREFLGDYDQDRVKFMVTQKVGDEYEEGMDILRDLYA 244 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 D R VPLSEL +GLKCGGSDGFSGITANPLLG FSDF+I+QGGTSVLTEVPEMFG Sbjct: 245 KASKDERTEVPLSELRVGLKCGGSDGFSGITANPLLGMFSDFLIAQGGTSVLTEVPEMFG 304 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 AE +LM R EN+++FE V LIN+FK+YFLS+GEPV ENPSPGNKAGGISTLE+K+LGCT Sbjct: 305 AETILMNRCENKDLFEQTVHLINDFKEYFLSHGEPVGENPSPGNKAGGISTLEEKALGCT 364 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 QK G S V V+ YG +L++KGL+LL APGN C +VLFTTGRGTPFG+F Sbjct: 365 QKCGKSYVSGVMPYGERLQKKGLNLLSAPGNDLVAATTLAASGCHMVLFTTGRGTPFGTF 424 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 VPT+K++TN+ L K W+DFNAG ++E PM E FI Y+I VASG+ NE+ Sbjct: 425 VPTMKISTNSTLAKNKPGWIDFNAGVIVENEPMEKTCERFIEYVIKVASGEFVNNEKKGY 484 Query: 480 RELAIFKTGVTL 491 RE+AIFKTGVTL Sbjct: 485 REIAIFKTGVTL 496 >ref|NP_755720.1| Altronate hydrolase [Escherichia coli CFT073] gb|AAN82294.1| Altronate hydrolase [Escherichia coli CFT073] Length = 495 Score = 560 bits (1443), Expect = e-158, Method: Compositional matrix adjust. Identities = 270/492 (54%), Positives = 349/492 (70%), Gaps = 1/492 (0%) Query: 1 MKMNPRDTVAVALRPIASGEVLSFEGLSLTAAQDIPQGHKIALTSFAEGDVITKYGYPIG 60 +K++ D VAVAL +A G +S + ++T QD+ +GHK ALT A+G + KYG PIG Sbjct: 4 IKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKFALTDIAKGANVIKYGLPIG 63 Query: 61 HAVEEIRAGDWIHTHNIKTNLSGEEEYEYLPDLHPVTYPKRGLTFQGYRRANGKVGIRND 120 +A+ +I AG+ +H HN +TNLS ++Y Y PD + Q YRRANG VG+RN+ Sbjct: 64 YALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQAADREVQIYRRANGDVGVRNE 123 Query: 121 LFIIPTVGCVNGIAEQMLQEFKAEHPDLGGFDNFTVLKHPYGCSQLGDDHRMTRSILLDA 180 L+I+PTVGCVNGIA Q+ F E + G D + H YGCSQLGDDH TR++L + Sbjct: 124 LWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLFSHTYGCSQLGDDHINTRTMLQNM 183 Query: 181 VNHPNAGGVLVFGLGCENNIVSEFRSMLGDYDGSRVKFLVAQEVGDEVXXXXXXXXXXXX 240 V HPNAG VLV GLGCENN V+ FR LGD D RV F++ Q+ DE+ Sbjct: 184 VRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPERVHFMICQQQDDEIEAGIAHLHQLYS 243 Query: 241 XXKNDVREPVPLSELNIGLKCGGSDGFSGITANPLLGAFSDFIISQGGTSVLTEVPEMFG 300 +ND REP LSEL GL+CGGSDG SGITANP+LG FSD++I+ GGT+VLTEVPEMFG Sbjct: 244 VMRNDKREPGKLSELKFGLECGGSDGLSGITANPMLGRFSDYVIANGGTTVLTEVPEMFG 303 Query: 301 AEKMLMARAENREVFEDIVSLINNFKQYFLSYGEPVYENPSPGNKAGGISTLEDKSLGCT 360 AE++LM + FE +V+++N+FKQYF+++ +P+YENPSPGNKAGGI+TLEDKSLGCT Sbjct: 304 AEQLLMDHCRDEATFEKLVTMVNDFKQYFIAHDQPIYENPSPGNKAGGITTLEDKSLGCT 363 Query: 361 QKAGSSPVVDVLDYGVKLRRKGLSLLQAPGNXXXXXXXXXXXXCQLVLFTTGRGTPFGSF 420 QKAGSS VVDVL YG +L+ GL+LL APGN C +VLF+TGRGTP+G F Sbjct: 364 QKAGSSVVVDVLRYGERLKTPGLNLLSAPGNDAVATSALAGAGCHMVLFSTGRGTPYGGF 423 Query: 421 VPTVKVATNNDLFAKKGHWMDFNAGPLLE-RPMADVLEEFIAYIIDVASGQKTRNEQNEV 479 VPTVK+ATN++L AKK HW+DF+AG L+ + M +LEEFI I++ A+G++T NE+N+ Sbjct: 424 VPTVKIATNSELAAKKKHWIDFDAGQLIHGKAMPQLLEEFIDTIVEFANGKQTCNERNDF 483 Query: 480 RELAIFKTGVTL 491 RELAIFK+GVTL Sbjct: 484 RELAIFKSGVTL 495 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.138 0.408 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 727,366,486 Number of extensions: 30734487 Number of successful extensions: 62320 Number of sequences better than 1.0e-05: 521 Number of HSP's gapped: 61587 Number of HSP's successfully gapped: 534 Length of query: 491 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 361 Effective length of database: 404,274,708 Effective search space: 145943169588 Effective search space used: 145943169588 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)