BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0346.0009.p (473 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00036274.1| COG0246: Mannitol-1-phosphate/altronate dehyd... 542 e-153 ref|NP_389120.1| similar to tagaturonate reductase [Bacillus sub... 459 e-128 ref|NP_347332.1| Altronate oxidoreductase [Clostridium acetobuty... 452 e-126 ref|NP_994205.1| altronate oxidoreductase [Yersinia pestis biova... 444 e-123 ref|NP_707453.1| altronate oxidoreductase [Shigella flexneri 2a ... 444 e-123 >ref|ZP_00036274.1| COG0246: Mannitol-1-phosphate/altronate dehydrogenases [Enterococcus faecium] Length = 473 Score = 542 bits (1397), Expect = e-153, Method: Compositional matrix adjust. Identities = 258/452 (57%), Positives = 335/452 (74%) Query: 17 PERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGLMAEQDNLYTVLLN 76 P +++QFGEGNFMR F+DWQ+QQ+N Q LF G+ A++ P+ QG+G +M +QDNLYTV+L Sbjct: 15 PVKVLQFGEGNFMRGFIDWQIQQLNNQQLFKGNVAIIQPLEQGLGNMMNQQDNLYTVILQ 74 Query: 77 GIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTTEAGIAYQPEDRLE 136 G+ + V++ EIIT V ++INPY +++AYL+LAEND+L +I SNTTEAGI + D L Sbjct: 75 GLSNGEIVDTSEIITVVEQMINPYDEWDAYLSLAENDDLAYIVSNTTEAGIQFVETDNLT 134 Query: 137 DAPPSSFPAKLTALLYRRFELGKKGFVIIPCELIDRNGEKLKEIVRQYAAAWNLGEAFLQ 196 D PP SFP KLTA LYRRF+L K GF IIPCELIDRNGE+LKEI+ +YA W+L AF++ Sbjct: 135 DTPPQSFPGKLTAFLYRRFKLNKAGFTIIPCELIDRNGERLKEIILRYAEKWDLEPAFVE 194 Query: 197 WLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAEPFLFWVIEGPAWLSERL 256 WL+ EN FCCSLVDRIVPGYPR A Y D+LMV AEPF+ WVIEGP L +L Sbjct: 195 WLEKENVFCCSLVDRIVPGYPREDAGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQL 254 Query: 257 PLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLETVEDVMNDETFYRFVRR 316 P +AGLNV++TDDMT YRERKVHLLNGPHTAMVPLA++A LETVE+VM D F +V + Sbjct: 255 PFKQAGLNVIITDDMTPYRERKVHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQ 314 Query: 317 LLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLNSISKFKTRLLPVLLRYQ 376 L + ELIP+L LP++EL YAD + ERF NPF H+L +ISLNS+SKF TRLLPV +Y Sbjct: 315 LFNLELIPMLSLPKDELAIYADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYI 374 Query: 377 QERGQLPPLITLAFAGLLLSYRGDRVKRQDSAEVLAVFDQAWSQPSTFTASILREVSLWG 436 +E+ Q+PPLIT++ A LLL YRGD+VK D + ++ F AWS T +L+ +LWG Sbjct: 375 EEQNQVPPLITVSLAALLLMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWG 434 Query: 437 QDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 +DL+Q+P +T+ V ++ R+ LQ++ Sbjct: 435 EDLSQIPNVTDTVQEIAAKIDQKGVRSVLQEM 466 >ref|NP_389120.1| similar to tagaturonate reductase [Bacillus subtilis] sp|O34354|UXAB_BACSU Altronate oxidoreductase (Tagaturonate reductase) (Tagaturonate dehydrogenase) pir||C69853 probable tagaturonate reductase (EC 1.1.1.58) - Bacillus subtilis gb|AAC46334.1| hypothetical altronate oxidoreductase [Bacillus subtilis] emb|CAB13095.1| tagaturonate reductase (altronate oxidoreductase) [Bacillus subtilis subsp. subtilis str. 168] Length = 480 Score = 459 bits (1180), Expect = e-128, Method: Compositional matrix adjust. Identities = 231/473 (48%), Positives = 302/473 (63%), Gaps = 11/473 (2%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 Q+L++ QYPE+++QFGEGNF+R F+DWQ+ Q+NQ FNGS AVV P G Sbjct: 2 QKLNKNVYDHYTQYPEKILQFGEGNFLRGFIDWQIDQLNQHTDFNGSVAVVQPRGSEKIK 61 Query: 63 LMAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNT 122 + EQD LYT+ L G+ + VN II S+SR I+ +SDYEAY LA ++ L FI SNT Sbjct: 62 RLNEQDGLYTLFLQGMKDGEAVNEHMIINSISRGIDLFSDYEAYKELASSERLRFIISNT 121 Query: 123 TEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFELGK----KGFVIIPCELIDRNGEKLK 178 TEAGI +DRLED P +FP KLTA LY R++ K KG V+IPCELI+ NGEKL+ Sbjct: 122 TEAGIVCDEKDRLEDRPQKTFPGKLTAFLYFRYQAFKGDQTKGCVLIPCELIENNGEKLR 181 Query: 179 EIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTA 238 E V YA W L E F QW+ + NTFC SLVDRIVPG+P E+ A+LGY D L+V Sbjct: 182 ETVLHYAHLWKLEEGFTQWIHEANTFCNSLVDRIVPGFPVDSIDEITADLGYQDDLIVVG 241 Query: 239 EPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGL 298 E + WVIEGP W+ + LP A AGL+ + D+T YR +KV +LNG HTAM P+A++ GL Sbjct: 242 EQYYLWVIEGPDWIGKELPFAAAGLHTKIVSDLTPYRTKKVRILNGAHTAMTPVALLYGL 301 Query: 299 ETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISL 358 +TV D + RF+R L+D+E++PVL + E L YAD VL RFKNP+I+H L SI+L Sbjct: 302 KTVRDAVEHPEVGRFIRELIDDEILPVLKM--EGLSQYADDVLNRFKNPYIKHYLESIAL 359 Query: 359 NSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKRQDSAEVLAVFDQAW 418 N+ISKFKTR LP L Y +++GQLP + +F+ LL Y D QD VL F + W Sbjct: 360 NAISKFKTRNLPTLKEYAEQKGQLPERLVFSFSALLYFYH-DNETLQDDPAVLQFFKEVW 418 Query: 419 SQPS----TFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQ 467 Q + +L E LWG DL ++P LT+ V L+ + + AL+Q Sbjct: 419 CQEDGDMLRIASRVLGEQRLWGADLNEIPKLTDRVAVYLNHIHELGMQRALEQ 471 >ref|NP_347332.1| Altronate oxidoreductase [Clostridium acetobutylicum] sp|Q97L67|UXAB_CLOAB Altronate oxidoreductase (Tagaturonate reductase) (Tagaturonate dehydrogenase) pir||E96985 altronate oxidoreductase CAC0695 [imported] - Clostridium acetobutylicum gb|AAK78672.1| Altronate oxidoreductase [Clostridium acetobutylicum] Length = 482 Score = 452 bits (1164), Expect = e-126, Method: Compositional matrix adjust. Identities = 220/481 (45%), Positives = 312/481 (64%), Gaps = 15/481 (3%) Query: 4 RLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGGL 63 +L+R+ +YPE+++QFGEGNF+RAFVDWQ+ +MN+ FN +V P+ QG+ + Sbjct: 2 KLNRSNFSEFKKYPEKILQFGEGNFLRAFVDWQVDRMNKAADFNAGVVIVQPLEQGLVDM 61 Query: 64 MAEQDNLYTVLLNGIMQEQPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSNTT 123 + EQD LYT+ L G+ + + V +I V+R +NPY+ YE YL LAEN E+E + SNTT Sbjct: 62 LNEQDGLYTLYLQGLKEGKAVKEHSVIDCVTRGLNPYTQYEEYLKLAENPEMEIVISNTT 121 Query: 124 EAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFEL----GKKGFVIIPCELIDRNGEKLKE 179 EAGIA+ D+L +S+P KLTA LYRRF+ KG +IIPCELIDRNGEKLK Sbjct: 122 EAGIAFDENDKLGRTCQNSYPGKLTAFLYRRFKTFNGDKSKGMLIIPCELIDRNGEKLKA 181 Query: 180 IVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVTAE 239 + ++A WNL + F W+++ NTFCC+LVDRIVPGYPR E+ AELGY D L+ E Sbjct: 182 TILKFAELWNLEKEFADWIEEANTFCCTLVDRIVPGYPRDTIEEVHAELGYEDSLVDVGE 241 Query: 240 PFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAGLE 299 F WVIEGP W+ + LP KAG+N+ V D+T YR RKV +LNG HTA+VP+A + GL+ Sbjct: 242 QFHLWVIEGPEWIKDILPFEKAGVNIQVVKDVTPYRTRKVRILNGAHTALVPVAYLYGLD 301 Query: 300 TVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASISLN 359 TV + D+ +++ L+ +E+IP LDLP+EEL +A AVLERF NPF+ H L SI+LN Sbjct: 302 TVGHSVEDKVIGKYLTELVYDEIIPTLDLPEEELKYFAGAVLERFLNPFVNHYLMSIALN 361 Query: 360 SISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDR----VKRQDSAEVLAVFD 415 S+ KF+TR LP LL Y++ +GQLP + + A L+ Y+G R +K D ++L ++ Sbjct: 362 SMPKFETRDLPSLLEYKKRKGQLPKKLVFSLAALIEFYKGKRGDEDIKLADGEDILELYK 421 Query: 416 QAWSQPSTFTA-------SILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQQL 468 WS+ A ++L W +L ++ GLT+AV L ++ + A++ + Sbjct: 422 DLWSKFDGTDACYRNIVETVLGYEKNWKMNLNELEGLTDAVTENLIKIDKLGMKEAVKSV 481 Query: 469 V 469 + Sbjct: 482 M 482 >ref|NP_994205.1| altronate oxidoreductase [Yersinia pestis biovar Mediaevails str. 91001] gb|AAS63082.1| altronate oxidoreductase [Yersinia pestis biovar Mediaevails str. 91001] Length = 483 Score = 444 bits (1143), Expect = e-123, Method: Compositional matrix adjust. Identities = 228/479 (47%), Positives = 314/479 (65%), Gaps = 17/479 (3%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 Q L+R PG +P+++IQFGEGNF+RAFVDWQ+ +N+ N V+ PI Sbjct: 2 QTLNRRDFPG-RSHPDKIIQFGEGNFLRAFVDWQIDLLNEHTDLNAGIVVIRPIDTDFPP 60 Query: 63 LMAEQDNLYTVLLNGIMQE-QPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSN 121 ++ QD LYT ++ G+ ++ + V +I SV+R IN Y ++ YLALA + + F+ SN Sbjct: 61 SLSTQDGLYTAVIRGLNEQGEAVRESRLIRSVNREINIYRQFDDYLALARDANIRFMFSN 120 Query: 122 TTEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFE----LGKKGFVIIPCELIDRNGEKL 177 TTEAGIA+ D+ DAPPSSFPAKLT LL+ RFE KG+V++PCELID NGE L Sbjct: 121 TTEAGIAWNEADQFSDAPPSSFPAKLTRLLFERFEHFDGAADKGWVLLPCELIDYNGEAL 180 Query: 178 KEIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVT 237 +E+V +YA+ W L AF WL + NTFC +LVDRIV GYPR + A L+ ELGY D + T Sbjct: 181 RELVLRYASHWQLPAAFTHWLTENNTFCSTLVDRIVTGYPRDEVAALQTELGYQDSFLDT 240 Query: 238 AEPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAG 297 AE F +VI+GP L++ L L + LNV + DD+ Y+ERKV +LNG HTA+VP+A ++G Sbjct: 241 AEYFYLFVIQGPQGLAQELRLDQLDLNVRIVDDIKPYKERKVAILNGAHTALVPVAYLSG 300 Query: 298 LETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASIS 357 L+TV M+D RFV + + EE++PVLDLP++ELLS++ AVL RF+NPFI+H+L SI+ Sbjct: 301 LDTVGQTMDDAQISRFVEKTITEEIVPVLDLPEDELLSFSQAVLSRFRNPFIQHQLLSIA 360 Query: 358 LNSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKR----QDSAEVLAV 413 LN ++KF+TR+LP LL YQQ++GQLPP +T A A L+ YRG+R + QD A L Sbjct: 361 LNGMTKFRTRILPQLLTYQQQKGQLPPRLTFALAALIAFYRGEREGQTYPLQDDAHWLER 420 Query: 414 FDQAWSQP-------STFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAAL 465 + W+ + +L + + WGQDLT VP L V +L + S RAA+ Sbjct: 421 YSTLWNGVKHGDIALAELVNRVLSDANHWGQDLTSVPQLANQVTEQLQTILSRGMRAAV 479 >ref|NP_707453.1| altronate oxidoreductase [Shigella flexneri 2a str. 301] sp|Q83RE1|UXAB_SHIFL Altronate oxidoreductase (Tagaturonate reductase) (Tagaturonate dehydrogenase) gb|AAN43160.1| altronate oxidoreductase [Shigella flexneri 2a str. 301] Length = 483 Score = 444 bits (1143), Expect = e-123, Method: Compositional matrix adjust. Identities = 235/483 (48%), Positives = 312/483 (64%), Gaps = 17/483 (3%) Query: 3 QRLSRTTQPGLPQYPERMIQFGEGNFMRAFVDWQLQQMNQQGLFNGSAAVVVPIGQGIGG 62 + L+R PG QYPER+IQFGEGNF+RAFVDWQ+ +N+ N VV PI Sbjct: 2 KTLNRRDFPG-AQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDLNSGVVVVRPIETSFPP 60 Query: 63 LMAEQDNLYTVLLNGIMQE-QPVNSREIITSVSRVINPYSDYEAYLALAENDELEFITSN 121 ++ QD LYT ++ G+ ++ + V+ +I SV+R I+ YS+Y+ +L LA N E+ F+ SN Sbjct: 61 SLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEYDEFLKLAHNPEMRFVFSN 120 Query: 122 TTEAGIAYQPEDRLEDAPPSSFPAKLTALLYRRFELGK----KGFVIIPCELIDRNGEKL 177 TTEAGI+Y D+ +DAP S+PAKLT LL+ RF KG++IIPCELID NG+ L Sbjct: 121 TTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGALDKGWIIIPCELIDYNGDAL 180 Query: 178 KEIVRQYAAAWNLGEAFLQWLDDENTFCCSLVDRIVPGYPRGKAAELEAELGYLDKLMVT 237 +E+V +YA W L EAF+QWLD N+FC +LVDRIV GYPR + A+LE ELGY D + T Sbjct: 181 RELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTGYPRDEVAKLEEELGYHDDFLDT 240 Query: 238 AEPFLFWVIEGPAWLSERLPLAKAGLNVVVTDDMTLYRERKVHLLNGPHTAMVPLAMMAG 297 AE F +VI+GP L+ L L K LNV++ DD+ Y+ERKV +LNG HTA+VP+A AG Sbjct: 241 AEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKPYKERKVAILNGAHTALVPVAFQAG 300 Query: 298 LETVEDVMNDETFYRFVRRLLDEELIPVLDLPQEELLSYADAVLERFKNPFIRHELASIS 357 L+TV + MND FV + + EE+IPVLDLP++EL S+A AV RF+NP+I+H+L SI+ Sbjct: 301 LDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRDELESFASAVTGRFRNPYIKHQLLSIA 360 Query: 358 LNSISKFKTRLLPVLLRYQQERGQLPPLITLAFAGLLLSYRGDRVKR----QDSAEVLAV 413 LN ++KF+TR+LP LL Q+ G LP +T A A L+ YRG+R QD A L Sbjct: 361 LNGMTKFRTRILPQLLAGQKANGTLPARLTFALAALIAFYRGERNGETYPVQDDAHWLER 420 Query: 414 FDQAWSQ-------PSTFTASILREVSLWGQDLTQVPGLTEAVDARLHQLQSSDSRAALQ 466 + Q WSQ A +L E W QDLTQVPGL E V L + R A++ Sbjct: 421 YQQLWSQHRDRVIGTQELVAIVLAEKDHWEQDLTQVPGLVEQVANDLDAILEKGMREAVR 480 Query: 467 QLV 469 L Sbjct: 481 PLC 483 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 684,061,748 Number of extensions: 28794520 Number of successful extensions: 66443 Number of sequences better than 1.0e-05: 183 Number of HSP's gapped: 66075 Number of HSP's successfully gapped: 183 Length of query: 473 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 344 Effective length of database: 406,989,807 Effective search space: 140004493608 Effective search space used: 140004493608 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)