BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0345.0033.p (454 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_655284.1| UPF0013, Uncharacterized membrane protein famil... 461 e-128 ref|NP_977822.1| MATE efflux family protein [Bacillus cereus ATC... 458 e-127 ref|NP_831164.1| Na+ driven multidrug efflux pump [Bacillus cere... 450 e-125 ref|NP_389826.1| similar to hypothetical proteins [Bacillus subt... 445 e-124 ref|NP_241726.1| BH0860~unknown conserved protein [Bacillus halo... 444 e-123 >ref|NP_655284.1| UPF0013, Uncharacterized membrane protein family UPF0013 [Bacillus anthracis A2012] ref|NP_843859.1| MATE efflux family protein [Bacillus anthracis str. Ames] gb|AAP25345.1| MATE efflux family protein [Bacillus anthracis str. Ames] Length = 453 Score = 461 bits (1186), Expect = e-128, Method: Compositional matrix adjust. Identities = 235/446 (52%), Positives = 297/446 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T S QK +QF+ + FPI +TQ++L A++FFDT MSG DLAGVAIGTS+WIP+ Sbjct: 1 MKETNSFSQKLKQFVLLFFPIFVTQMSLFAMSFFDTTMSGHASPIDLAGVAIGTSIWIPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM TP+V+ L+GSKK+ DV + + Q ++ G F ++P+L+ M Sbjct: 61 STGLTGILMATTPIVAQLVGSKKKEDVPHVIIQAVYLAICASFVVILIGLFTVTPILNGM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 LE V IA +FL I++GIIPLF YTV+R IDALG+TR +M ITL++LP+NV LNY+ Sbjct: 121 RLEEPVERIAAQFLSIIAIGIIPLFTYTVLRGFIDALGKTRTTMIITLLSLPINVVLNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFG FGFP+LGGVGA +ASA TYW I I +M + EP KE Sbjct: 181 LIFGNFGFPKLGGVGAAIASAATYWCILIITVMIIRTKEPFASFNIFKQLYRPSLSSWKE 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LK+GVPIGF+IFFET++F+AVTL+MS F T TIAAHQAA+NFAS LYM PLS+ M++TI Sbjct: 241 FLKLGVPIGFAIFFETSIFAAVTLMMSNFSTTTIAAHQAAMNFASLLYMTPLSLAMAMTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VGFE GA R +A+QYG +GIG +A +Y+ + +V L + F Sbjct: 301 AVGFEVGAKRYNNAKQYGFIGIGLALAFALLYSILLYFFDDEIASIYTTDIQVHHLAKEF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LI+AI FQISDAIATP QG LRGYKDVN A I+ IAYWVIGLP GY+LATYT AA GY Sbjct: 361 LIFAILFQISDAIATPVQGALRGYKDVNVALIMTLIAYWVIGLPLGYILATYTDWAAKGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQY 446 W+ RL +VQR+Y Sbjct: 421 WIGLIIGLAFGATFLLIRLFQVQRKY 446 >ref|NP_977822.1| MATE efflux family protein [Bacillus cereus ATCC 10987] gb|AAS40430.1| MATE efflux family protein [Bacillus cereus ATCC 10987] Length = 454 Score = 458 bits (1179), Expect = e-127, Method: Compositional matrix adjust. Identities = 235/446 (52%), Positives = 297/446 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T++ QK +QF+ + FPI ITQ++L A++FFDT MSG DLAGVAIGTS+WIP+ Sbjct: 1 MKETSTFSQKLKQFVLLFFPIFITQMSLFAMSFFDTTMSGHASPIDLAGVAIGTSIWIPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM TP+V+ L+GSKK+ DV V Q ++ G FA++P+L+ M Sbjct: 61 STGLTGILMATTPIVAQLVGSKKKEDVPQVVIQAVYLAICASFVVMLIGFFAVTPILNGM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 LE V IA +FL I++GIIPLF YTV+R IDALG+TR +M ITL++LP+NV LNY+ Sbjct: 121 RLEEPVERIAAQFLSIIAIGIIPLFTYTVLRGFIDALGKTRTTMIITLLSLPINVILNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFG FGFP+LGGVGA +AS TYW I I +M + EP KE Sbjct: 181 LIFGHFGFPKLGGVGAAIASTATYWCILIITVMIIRTKEPFASFHIFKQLYRPSLSSWKE 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LK+GVPIGF+IFFET++F+AVTL+MS F T TIAAHQAA+NFAS LYM PLS+ M++TI Sbjct: 241 FLKLGVPIGFAIFFETSIFAAVTLMMSNFSTTTIAAHQAAMNFASLLYMTPLSLAMAMTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VGFE GA R +A+QYG +GIG +A +Y+ + +V L + F Sbjct: 301 AVGFEVGAKRYNNAKQYGFIGIGLALAFALLYSILLYFFDDEIASIYTTDIQVHHLAKEF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LI+AI FQISDAIATP QG LRGYKDVN + I+ IAYWVIGLP GY+LATYT AA GY Sbjct: 361 LIFAILFQISDAIATPVQGALRGYKDVNVSLIMTLIAYWVIGLPLGYILATYTDWAAKGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQY 446 W+ RL +VQR+Y Sbjct: 421 WIGLIIGLAFGATFLLIRLFQVQRKY 446 >ref|NP_831164.1| Na+ driven multidrug efflux pump [Bacillus cereus ATCC 14579] gb|AAP08365.1| Na+ driven multidrug efflux pump [Bacillus cereus ATCC 14579] Length = 452 Score = 450 bits (1157), Expect = e-125, Method: Compositional matrix adjust. Identities = 233/446 (52%), Positives = 296/446 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M TTS QK +QF+ + FPI +TQ++L A++FFDT MSG DL+GVAIGTS+WIP+ Sbjct: 1 MKETTSFSQKLKQFVLLFFPIFVTQMSLFAMSFFDTTMSGHASPTDLSGVAIGTSIWIPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM TP+V+ L+GSKK+ DV + V Q ++ G F +SP+L+ M Sbjct: 61 STGLTGILMATTPIVAQLVGSKKKEDVPHVVIQAVYLAICASFVVILIGFFVVSPILNGM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 LE V IA +FL I++GIIPLF YTV+R IDALG+TR +M ITL++LP+NV LNYL Sbjct: 121 RLEEPVERIAAQFLSIIAIGIIPLFTYTVLRGFIDALGKTRTTMIITLLSLPINVILNYL 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFG FGFP+LGGVGA +AS TYW I I ++ + EP Sbjct: 181 LIFGNFGFPKLGGVGAAIASTATYWCILIITVIIIQTKEPFASFNIFKQLYRPSLSSWIA 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LK+GVPIGF+IFFET++F+AVTL+MS F T TIAAHQAA+NFAS LYM PLS+ M++TI Sbjct: 241 FLKLGVPIGFAIFFETSIFAAVTLMMSNFSTTTIAAHQAAMNFASLLYMTPLSLAMAMTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VGFE GA R +A+QYG++GIG +A +Y+ + +V L + F Sbjct: 301 AVGFEVGAKRYDNAKQYGLIGIGLALAFALLYSILLYFFDDQIASIYTTDAKVHHLAKEF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LI+AI FQISDAIATP QG LRGYKDVN A I+ IAYW+IGLP GY+LATYT AA GY Sbjct: 361 LIFAILFQISDAIATPVQGALRGYKDVNVALIMTLIAYWIIGLPLGYILATYTEWAAKGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQY 446 W+ RL +VQR+Y Sbjct: 421 WIGLIIGLAFGAAFLLIRLFQVQRKY 446 >ref|NP_389826.1| similar to hypothetical proteins [Bacillus subtilis] sp|O31855|NORM_BACSU Probable multidrug resistance protein norM (Na(+)/drug antiporter) (Multidrug-efflux transporter) pir||F69906 conserved hypothetical protein yojI - Bacillus subtilis emb|CAB13836.1| yojI [Bacillus subtilis subsp. subtilis str. 168] gb|AAC17857.1| YojI [Bacillus subtilis] Length = 452 Score = 445 bits (1145), Expect = e-124, Method: Compositional matrix adjust. Identities = 224/450 (49%), Positives = 299/450 (66%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M T + ++K +Q L+IL PI ITQ LS ITF DT MSGK DLAGVAIG+SLW P+ Sbjct: 1 MKETQNAREKIKQLLHILIPIFITQAGLSLITFLDTVMSGKVSPADLAGVAIGSSLWTPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+GILM +TP+V+ L+G++K+ + + V Q ++ G A+ +L + Sbjct: 61 YTGLAGILMAVTPIVAQLLGAEKKQKIPFTVLQAVYVAALLSIAVLVIGYAAVDLILGRL 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 NL+ VH IA FL +S+GI PLF YTV+RS ID+LG+TRV+M ITL +LP+N LNY+ Sbjct: 121 NLDIHVHQIAKHFLGFLSLGIFPLFVYTVLRSFIDSLGKTRVTMMITLSSLPINFVLNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 IFGKFG P LGGVGAG+ASA+TYW I I+ +++ P K Sbjct: 181 FIFGKFGMPALGGVGAGLASALTYWCICIISFFIIHKNAPFSEYGIFLTMYKFSWKACKN 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 LLKIG+PIGF++FFET++F+AVTLLMS F T+TIA+HQAA+NFAS LYM+PLS+ M+LTI Sbjct: 241 LLKIGLPIGFAVFFETSIFAAVTLLMSHFHTVTIASHQAAMNFASLLYMLPLSVSMALTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 +VGFE GA R KDAR Y ++GI +AG+Y+ +P+V+ L +HF Sbjct: 301 VVGFEAGAARFKDARSYSLIGIMMAIGFSLFTAACILLFREQIAGMYTSDPDVLRLTQHF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LIYA+FFQ+SDA+A P QG LRGYKDVN F++YWVIGLP GY++ T+TSL A+GY Sbjct: 361 LIYALFFQLSDAVAAPIQGALRGYKDVNYTLAAAFVSYWVIGLPVGYMVGTFTSLGAFGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAHNK 450 W+ RL K+Q++Y+ + Sbjct: 421 WIGLIAGLAAGAVGLFFRLAKLQKRYSQKQ 450 >ref|NP_241726.1| BH0860~unknown conserved protein [Bacillus halodurans] sp|Q9KEJ2|NORM_BACHD Probable multidrug resistance protein norM (Na(+)/drug antiporter) (Multidrug-efflux transporter) pir||D83757 hypothetical protein BH0860 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04579.1| BH0860~unknown conserved protein [Bacillus halodurans] Length = 458 Score = 444 bits (1141), Expect = e-123, Method: Compositional matrix adjust. Identities = 224/448 (50%), Positives = 305/448 (68%) Query: 1 MIPTTSIKQKARQFLYILFPILITQIALSAITFFDTNMSGKFGTDDLAGVAIGTSLWIPI 60 M PT ++++K + FL ++ PILITQI L A+ FFDT MSG+ G +DLAGVAIG+SLW+P+ Sbjct: 1 MKPTETLQEKGKLFLVVMMPILITQIGLYAMNFFDTVMSGQAGANDLAGVAIGSSLWVPV 60 Query: 61 QTGLSGILMGITPLVSHLMGSKKEGDVAYQVTQGIWXXXXXXXXXXXXGTFALSPVLHFM 120 TGL+G+L+ +TP+++ +G++K DV Y QG++ G L P+L M Sbjct: 61 FTGLNGVLLALTPIIAQSIGAEKRDDVPYVFLQGLYLSIAISIAVILIGAVVLDPILSAM 120 Query: 121 NLEPQVHDIAFRFLCAISVGIIPLFGYTVIRSCIDALGQTRVSMFITLIALPVNVGLNYL 180 +LE +V IA +L ++ GI+PLF YT IR ID+LG+TRV+MFITL++LP+N+ NY+ Sbjct: 121 SLEDEVGRIAKEYLIGLAFGIVPLFIYTTIRCLIDSLGETRVTMFITLLSLPINIFFNYV 180 Query: 181 LIFGKFGFPRLGGVGAGVASAITYWVIFGIALMFVYRTEPXXXXXXXXXXXXXXXXXXKE 240 LIFGK GFPRLGGVGAG ASAITYW I +A++ V + P KE Sbjct: 181 LIFGKLGFPRLGGVGAGYASAITYWFILAVAIVVVVKVRPFTDFQLFKKLYHVSLKKWKE 240 Query: 241 LLKIGVPIGFSIFFETAVFSAVTLLMSRFDTITIAAHQAAINFASTLYMIPLSICMSLTI 300 +L +G+PIGF+IFFET++F+AVTLLMS FDT TIAAHQAA+NFAS LYMIPLSI +LTI Sbjct: 241 ILLLGLPIGFTIFFETSIFAAVTLLMSTFDTATIAAHQAAVNFASFLYMIPLSIAFTLTI 300 Query: 301 LVGFENGAGRLKDARQYGIMGIGXXXXXXXXXXXXXXXXGSHVAGLYSDEPEVISLIRHF 360 VG+E GA R++DARQY +GI + VA LY+++ +V LI+ F Sbjct: 301 AVGYEVGAKRVEDARQYSRLGITFALIMGLVAGVIIYVLRAPVASLYTNDSQVAWLIQQF 360 Query: 361 LIYAIFFQISDAIATPTQGVLRGYKDVNPAFIICFIAYWVIGLPTGYVLATYTSLAAYGY 420 LIY+IFFQ+SDA+ATP QGVLRG+KDVN F++ +++W+IGLPTGY+LA ++ L YGY Sbjct: 361 LIYSIFFQLSDALATPIQGVLRGHKDVNVPFVMALVSFWIIGLPTGYLLANFSPLGPYGY 420 Query: 421 WVXXXXXXXXXXXXXXSRLVKVQRQYAH 448 W+ RL ++QR++ Sbjct: 421 WIGLITGLASCAIALSWRLKQMQRKFER 448 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.328 0.143 0.436 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 570,330,370 Number of extensions: 21397831 Number of successful extensions: 58902 Number of sequences better than 1.0e-05: 482 Number of HSP's gapped: 58215 Number of HSP's successfully gapped: 488 Length of query: 454 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 325 Effective length of database: 406,989,807 Effective search space: 132271687275 Effective search space used: 132271687275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.8 bits) S2: 128 (53.9 bits)