BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0342.0057.p (303 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] 287 2e-76 ref|ZP_00099614.1| COG1131: ABC-type multidrug transport system,... 281 2e-74 ref|NP_241311.1| ABC transporter (ATP-binding protein) [Bacillus... 273 4e-72 emb|CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728] 270 2e-71 pir||JC5348 cdd4 protein - Clostridium difficile >gi|1770140|emb... 266 5e-70 >emb|CAB37661.1| cdd4-like protein [Bacillus sphaericus] Length = 311 Score = 287 bits (734), Expect = 2e-76, Method: Compositional matrix adjust. Identities = 143/298 (47%), Positives = 213/298 (71%), Gaps = 3/298 (1%) Query: 3 QLIVETKGLTRRFGEQVSVNEVNLEVPEGKIYGFLGPNGAGKTTTIKMLLGLLRADAGTI 62 + IV+T+ L++ FG++ +V+ +NL++ +G+IYGFLGPNGAGKTTTI+MLLGL++ +GTI Sbjct: 10 EYIVQTQNLSKNFGKEQAVSNINLQIRKGEIYGFLGPNGAGKTTTIRMLLGLMKPSSGTI 69 Query: 63 RMFSQEMPENSLDILRKVGSLVENPSYYGHLTGYKNLKILSTLLKLPDRRIGEVLEIVRL 122 ++F +++ ++ + IL VGSLVENPSYY HLT Y+NL+ L +L +P RI EVL+ VRL Sbjct: 70 KIFQKDIVKDRIQILANVGSLVENPSYYPHLTAYENLEALRKILGVPKTRIDEVLQTVRL 129 Query: 123 TSSADRKVSGYSLGMKQRLGIAAALLRGPQLLILDEPTNGLDPAGIQEIRHLMLSLAHDQ 182 +A++KV G+SLGMKQRLGIAAALL P+LLILDEPTNGLDP+GI EIR+L+ L + Sbjct: 130 KEAANKKVKGFSLGMKQRLGIAAALLHNPELLILDEPTNGLDPSGIIEIRNLIKRLPKEY 189 Query: 183 GMTILLSSHLLSEVDTIADEIGIISSGRLVYQGALELLERRRGKVLEFAVERPEEALRTL 242 GMTI++SSHLLSE+D +A ++GI+S G L++Q ++E + + + F V E+ R+L Sbjct: 190 GMTIIISSHLLSEIDQMATQVGIVSKGTLIFQDSIEGMRKFAQPKVTFKVSNGEKGWRSL 249 Query: 243 LLQGFTVERKGNTLSVAAENASMTQAELFKLLGPYGI--MNAREHSKSLEELFMELTG 298 L G + + + + E + A + ++L GI E +SLE++F+++T Sbjct: 250 LANGIKANYQDDVIFL-EECSDEKVAHIVQVLVQEGISVFRVEEEKRSLEDIFLQMTA 306 >ref|ZP_00099614.1| COG1131: ABC-type multidrug transport system, ATPase component [Desulfitobacterium hafniense] Length = 304 Score = 281 bits (718), Expect = 2e-74, Method: Compositional matrix adjust. Identities = 146/301 (48%), Positives = 211/301 (70%), Gaps = 2/301 (0%) Query: 5 IVETKGLTRRFGEQVSVNEVNLEVPEGKIYGFLGPNGAGKTTTIKMLLGLLRADAGTIRM 64 I++T+GL + +G+ V++++L V EG +YGFLGPNGAGK+TT+KM+LGL++ AG+I + Sbjct: 4 IIKTQGLCKHYGKTPGVSDLDLSVAEGAVYGFLGPNGAGKSTTLKMILGLVKPTAGSITV 63 Query: 65 FSQEMPENS-LDILRKVGSLVENPSYYGHLTGYKNLKILSTLLKLPDRRIGEVLEIVRLT 123 F + M S + IL+ VGSL+E+PSYYGHL+GY+NL+I++TL P++ IG VLEIVRL Sbjct: 64 FGKTMNHRSRMSILKDVGSLIESPSYYGHLSGYENLRIIATLKGTPEKDIGRVLEIVRLE 123 Query: 124 SSADRKVSGYSLGMKQRLGIAAALLRGPQLLILDEPTNGLDPAGIQEIRHLMLSLAHDQG 183 + ++K + YSLGMKQRLG+A ALL P+LLILDEPTNGLDPAGIQE+R L+ SL + G Sbjct: 124 NQKNKKTNQYSLGMKQRLGLACALLGNPRLLILDEPTNGLDPAGIQEMRELIRSLPKEYG 183 Query: 184 MTILLSSHLLSEVDTIADEIGIISSGRLVYQGALELLERRRGKVLEFAVERPEEALRTLL 243 +T+L+SSHLLSE++ +A +GIIS GRLV+Q +L L ++ + A L Sbjct: 184 ITVLVSSHLLSEIEQMATNLGIISKGRLVFQSSLGALHQKSSPSIAIRTLDNHRAAAVLR 243 Query: 244 LQGFTVERKGNTLSVAA-ENASMTQAELFKLLGPYGIMNAREHSKSLEELFMELTGKGVS 302 QG V+ + + L + A + +++ L G++ + KSLE++F+ELTG VS Sbjct: 244 EQGIAVQCQQDYLLLPAFADGELSRLTLNLFEQKVGVVRIEQREKSLEDIFLELTGSAVS 303 Query: 303 L 303 L Sbjct: 304 L 304 >ref|NP_241311.1| ABC transporter (ATP-binding protein) [Bacillus halodurans] pir||E83705 ABC transporter (ATP-binding protein) BH0445 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04164.1| ABC transporter (ATP-binding protein) [Bacillus halodurans] Length = 306 Score = 273 bits (697), Expect = 4e-72, Method: Compositional matrix adjust. Identities = 149/303 (49%), Positives = 215/303 (70%), Gaps = 7/303 (2%) Query: 5 IVETKGLTRRFGEQVSVNEVNLEVPEGKIYGFLGPNGAGKTTTIKMLLGLLRADAGTIRM 64 ++ET LT + +V+ +NL+V EG+IYGFLGPNGAGKTTTI+MLLGL++A G+I++ Sbjct: 7 LIETNNLTMTYKHVHAVDRINLKVKEGEIYGFLGPNGAGKTTTIRMLLGLIKASHGSIKI 66 Query: 65 FSQEMPENSLDILRKVGSLVENPSYYGHLTGYKNLKILSTLLKLPDRRIGEVLEIVRLTS 124 F +++ ++ LDIL+ +G+LVE+PSYY HL+G NL+ + ++K+P RI EVLE+VRLT Sbjct: 67 FGKDLKQHRLDILKNIGALVESPSYYPHLSGEDNLETVRKIVKVPKSRIAEVLELVRLTK 126 Query: 125 SADRKVSGYSLGMKQRLGIAAALLRGPQLLILDEPTNGLDPAGIQEIRHLMLSLAHDQGM 184 ADRKV YSLGMKQRL IAAALL P+L+ILDEPTNGLDPAGI EIR+L+L L + G+ Sbjct: 127 VADRKVQEYSLGMKQRLAIAAALLANPRLVILDEPTNGLDPAGIIEIRNLILRLPKEYGI 186 Query: 185 TILLSSHLLSEVDTIADEIGIISSGRLVYQGALELLERRRGKVLEFAVERPEEALRTLLL 244 T+LLSSHLLSEV+ +A ++GII+ G+L++Q ++E L ++ + + + PE A L Sbjct: 187 TVLLSSHLLSEVEQVASQVGIIAKGKLIFQDSIEELRKKANRKIFLSTSHPEVAATILQE 246 Query: 245 QGFTVERKGNTLSVA----AENASMTQAELFKLLGPYGIMNAREHSKSLEELFMELTGKG 300 +G V+ + L + E A + +A + K + Y I + + SLEE+F++LT G Sbjct: 247 RGINVDTQARGLVMNDRSDEEIAQLVKAFVEKDIDVYRI---SDETSSLEEIFLQLTKGG 303 Query: 301 VSL 303 +L Sbjct: 304 QTL 306 >emb|CAB60255.1| MrsF protein [Bacillus sp. HIL-Y85/54728] Length = 303 Score = 270 bits (691), Expect = 2e-71, Method: Compositional matrix adjust. Identities = 144/304 (47%), Positives = 209/304 (68%), Gaps = 2/304 (0%) Query: 1 MPQLIVETKGLTRRFGEQVSVNEVNLEVPEGKIYGFLGPNGAGKTTTIKMLLGLLRADAG 60 M IV T LT+++ + SV+ +NL + G+IYGFLGPNGAGKTTTI+MLLGL++ G Sbjct: 1 MNDQIVVTHDLTKKYKKHTSVDGLNLRIRRGEIYGFLGPNGAGKTTTIRMLLGLIKPTKG 60 Query: 61 TIRMFSQEMPENSLDILRKVGSLVENPSYYGHLTGYKNLKILSTLLKLPDRRIGEVLEIV 120 I +F Q + +N L IL+++GSLVE+P+YYG+LTGY+NL+ + L LP++++ EVLE V Sbjct: 61 NIEIFGQNLNKNRLQILQRIGSLVESPTYYGNLTGYENLEAVRRLRGLPEQQVNEVLETV 120 Query: 121 RLTSSADRKVSGYSLGMKQRLGIAAALLRGPQLLILDEPTNGLDPAGIQEIRHLMLSLAH 180 RL+ A+R YSLGMKQRLGIA ALL P LLILDEPTNGLDP+GIQEIR L+ L Sbjct: 121 RLSKVANRLTKEYSLGMKQRLGIAVALLSSPDLLILDEPTNGLDPSGIQEIRELIKELPK 180 Query: 181 DQGMTILLSSHLLSEVDTIADEIGIISSGRLVYQGALELLERRRGKVLEFAVERPEEALR 240 GM++++SSHLLSE+D +A ++GII++G++++Q ++ L ++R +L+ V EA Sbjct: 181 S-GMSVIVSSHLLSEIDQMATQVGIINNGKMIFQDSIASLHQKRKPLLKVGVSDVIEAKT 239 Query: 241 TLLLQGFTVERKGNTLSVA-AENASMTQAELFKLLGPYGIMNAREHSKSLEELFMELTGK 299 L +G V+ + N L ++ E +++ L + E ++SLE++F+ELTG Sbjct: 240 ILNRKGLKVDLQKNYLWLSQTEPEFVSEINSILLHSGLSVFRLEEKTRSLEDIFLELTGT 299 Query: 300 GVSL 303 SL Sbjct: 300 EGSL 303 >pir||JC5348 cdd4 protein - Clostridium difficile emb|CAA63569.1| cdd4 [Clostridium difficile] Length = 301 Score = 266 bits (679), Expect = 5e-70, Method: Compositional matrix adjust. Identities = 139/304 (45%), Positives = 201/304 (66%), Gaps = 18/304 (5%) Query: 3 QLIVETKGLTRRFGEQVSVNEVNLEVPEGKIYGFLGPNGAGKTTTIKMLLGLLRADAGTI 62 ++I+ T+ L++ + + V +++L VP+G +YGFLGPNGAGK+TT+KMLLGL + G I Sbjct: 2 EMIIRTQRLSKEYNKIFRVKDIDLRVPQGAVYGFLGPNGAGKSTTLKMLLGLAKPTQGNI 61 Query: 63 RMFSQEMPE-NSLDILRKVGSLVENPSYYGHLTGYKNLKILSTLLKLPDRRIGEVLEIVR 121 + +E+ E N + IL+ +GSL+E+PSYYGHLTG +N+ I+ LL LP + + E L+IVR Sbjct: 62 NILGKELNEKNRISILKDIGSLIESPSYYGHLTGLENMTIMQRLLDLPKKNVNEALKIVR 121 Query: 122 LTSSADRKVSGYSLGMKQRLGIAAALLRGPQLLILDEPTNGLDPAGIQEIRHLMLSLAHD 181 L + ++KVS YSLGMKQRLGIA A+++ P+LLILDEPTNGLDPAGI+EIR L+ SL Sbjct: 122 LENQKNKKVSQYSLGMKQRLGIAMAIMKFPKLLILDEPTNGLDPAGIEEIRELIKSLPEK 181 Query: 182 QGMTILLSSHLLSEVDTIADEIGIISSGRLVYQGALELLERRRGKVLEFAVERPEEALRT 241 GMT+L+SSHLLSE+D IA IGII+ G LV+Q +++ L R + + A + Sbjct: 182 YGMTVLISSHLLSEIDQIATSIGIINHGELVFQDSIKELHNRGQSQIAIKTKNVVSAQKL 241 Query: 242 LLLQGF---------TVERKGNTLSVAAENASMTQAELFKLLGPYGIMNAREHSKSLEEL 292 L +G+ T+E ++ VA N + + + + EH KSLE++ Sbjct: 242 LNTKGYMSLIQDGYLTIENLKDS-DVAKINGMLVHSNI-------DVFRIEEHKKSLEDI 293 Query: 293 FMEL 296 F+EL Sbjct: 294 FLEL 297 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 410,559,608 Number of extensions: 16111275 Number of successful extensions: 117243 Number of sequences better than 1.0e-05: 19525 Number of HSP's gapped: 98241 Number of HSP's successfully gapped: 20742 Length of query: 303 Length of database: 757,237,578 Length adjustment: 125 Effective length of query: 178 Effective length of database: 417,850,203 Effective search space: 74377336134 Effective search space used: 74377336134 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 125 (52.8 bits)