BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0028.p (698 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAA67014.1| hypothetical protein AN8392.2 [Aspergillus nidula... 528 e-148 ref|NP_822817.1| putative alpha-galactosidase [Streptomyces aver... 107 8e-22 ref|NP_561290.1| alpha-galactosidase [Clostridium perfringens] >... 107 1e-21 gb|AAF68953.1| alpha-galactosidase [Absidia corymbifera] 107 1e-21 ref|NP_822257.1| putative alpha-galactosidase [Streptomyces aver... 103 1e-20 >gb|EAA67014.1| hypothetical protein AN8392.2 [Aspergillus nidulans FGSC A4] Length = 732 Score = 528 bits (1360), Expect = e-148, Method: Compositional matrix adjust. Identities = 300/718 (41%), Positives = 401/718 (55%), Gaps = 32/718 (4%) Query: 1 MDSKRIDIAENGLYLTIEITGEQDVRLLHFGAAPLQDGSIAE--KNKPGFR--------L 50 M ++ I I N L +T+ I E GAA L+ + +P R L Sbjct: 22 MAAQTITIRSNALAVTVYIDNE--------GAAYLEPITPVRCTPRRPISRYFESHHAPL 73 Query: 51 LELQLSGEDRDEYHG-RTHRASYPGLRMVYGGHSDSVNALGRKLEITLTDPVTGITAVQH 109 +E++L+GE ++ ++ Y G R+ Y + + +ITL+DP T + H Sbjct: 74 VEVRLAGEGTQKHKSSKSLVGGYIGPRLRYRSSVIRDSEGSKTADITLSDPELNFTVIVH 133 Query: 110 LQFYTGVQTVRCWTVLKNTGTGEAAVEYVSTFALTGVDKEGSGDRNDKIEVTIAHSGWQS 169 L Y T+R T ++N E V V++ L G+ GS + D+ ++I + W Sbjct: 134 LTVYGSTPTLRATTTIRNHSDQELIVTQVTSLVLGGL-TTGSAEWWDEYTLSIPTNSWFR 192 Query: 170 ELQWRTYRLPELGMSHLADRGSKR--------IAASNTGSWSAAELLPMAVLHNKESGTS 221 E QW + LP LG+ G A SN G++S LPM +L +++G + Sbjct: 193 EAQWIEHDLPGLGLDDYGVYGRPENHFASLAHYAVSNRGTFSTEGHLPMGLLKRRDNGDT 252 Query: 222 LFWQIEHNGSWHWELTDQYDQLTLLVSGPTEHDNHWWLKLAPGEEFTSXXXXXXXXXXXX 281 WQ+E+NGSW WE+ D D + L GP E D+ W L L PGEE T+ Sbjct: 253 WLWQVENNGSWRWEIGDWKDSVYLAAGGPVETDHDWRLILQPGEESTTVPVALVHVYDDY 312 Query: 282 XXXXEQLTAYRRMIRRPNEDNELLRIIFNDYMNCLWGSPTTGKLLPLIDAAADVGCEYFC 341 LT YRR+IRR +EDN L IIFNDYMNCL G PT K+L L+D G EYF Sbjct: 313 EKAFSALTRYRRIIRRKHEDNSRLPIIFNDYMNCLMGDPTEEKILALVDPVIKAGAEYFV 372 Query: 342 IDAGWYA-PGEWWDGVGQWEPSAERFPEGIKYVLDYIRSKGMIPGLWLELEVMGINSPKL 400 ID GWYA WWD VG+WEPS +RFP G +L +R KG+IPGLW+E EV+G+ S Sbjct: 373 IDCGWYADEAGWWDDVGEWEPSNKRFPNGFSTLLAQLRKKGLIPGLWIEPEVIGVRSKVA 432 Query: 401 TETDDSWFFMRHGKRVKDRSRYQLDYRNPKVIAHADGVISRLVEQYGVGYIKMDYNINAG 460 + D FF R G+R+ ++ RYQLDYR+ V + G+I RLV ++GVGY K DYNI+ Sbjct: 433 AQLPDEAFFQRDGERILEKGRYQLDYRHSAVRSRMHGIIERLVIEFGVGYFKFDYNIDVT 492 Query: 461 IGTETDADSFGDGLLQHNRAYLAWLDSIFARYPNLVIENCSSGGMRMDYAMLSRHSIQST 520 GT+ S G L+HNRAYL W++ + RYP LV+ENCSSGG RMDYAMLS H IQS+ Sbjct: 493 QGTDISTSSPGAAQLEHNRAYLTWVNELLDRYPGLVVENCSSGGQRMDYAMLSTHPIQSS 552 Query: 521 SDQEDYVQYAAIAAGSPAALTPEQSAVWSYPLREGDDEEVIFNMVNALLLRVHQSGHLAE 580 SDQ+D V+YAAI+A P A+TPEQ AVW YP E DDE ++VNALL RVH SG L Sbjct: 553 SDQQDPVKYAAISAALPTAVTPEQGAVWVYPQPEWDDETNAMSVVNALLGRVHLSGRLDL 612 Query: 581 LEPRRRELVKEALDYYKSIRAHIPQATAFWPLGLPDSEGEWVSFGLR--HGDTRYLAVWR 638 L ++ LV E +D Y+SIR +P ATAFWPLGLP W+ G+ G+ Y+AVWR Sbjct: 613 LSEQQVSLVNEGMDIYRSIRGDLPNATAFWPLGLPGWHDGWIVLGMAVDGGERFYMAVWR 672 Query: 639 IAGEAAAVKLPIPELQGQAAGVSCAYPQRHHSDWSWNPSEGVLTVSIPQGKTARLFEI 696 G V LP P L+ + YP+ + + WN +EG L V + ARLF++ Sbjct: 673 RGGLDTVV-LPAPLLRARDLKAELLYPKAFATHFEWNVAEGSLRVGLASTLCARLFKL 729 >ref|NP_822817.1| putative alpha-galactosidase [Streptomyces avermitilis MA-4680] dbj|BAC69352.1| putative alpha-galactosidase [Streptomyces avermitilis MA-4680] Length = 700 Score = 107 bits (268), Expect = 8e-22, Method: Compositional matrix adjust. Identities = 134/535 (25%), Positives = 220/535 (41%), Gaps = 48/535 (8%) Query: 90 GRKLEITLTDPVTGITAVQHLQFYTGVQTVRCWTVLKNTGTGEAAVEYVSTFALTGVDKE 149 G +L + D +G+ H + V V W L N G A+E + + T E Sbjct: 113 GDELRLRFGD--SGLAVTLHYRMRDDV--VERWVTLANDG---PALELLRADSATWTLPE 165 Query: 150 GSGDRNDKIEVTIAHSGWQSELQWRTYRLPELGMSHLADRGSKRIAASNTGSWSAAELLP 209 G R ++ H W +E RL +++ ++I S G + + LP Sbjct: 166 RDGWRLSQL-----HGRWAAE-----SRLVRSDLTY-----GEKIIGSRRGH-TGHQHLP 209 Query: 210 MAVLH---NKESGTSLFWQIEHNGSWHWELTDQYDQLTLLVSGPTEHDNHWWLKLAPGEE 266 L +ESG + +GSW + Q + ++G +D+ L+L PGE Sbjct: 210 WVALDCEATEESGEVYGCALGWSGSWRISVA-QLSDARVQITGGAGYDDSGLLRLGPGES 268 Query: 267 FTSXXXXXXXXXXXXXXXXEQLTAYRRMIRRPNEDNELLRIIFNDYMNCLWGSPTTGKLL 326 FT+ AY+R P+ D + ++FN + + + + Sbjct: 269 FTTPVFAGLWSDGGFGGASRAWHAYQRAYVIPDADQDR-PVLFNSWEATTF-DISEEQQG 326 Query: 327 PLIDAAADVGCEYFCIDAGWYAP--GEWWDGVGQWEPSAERFPEGIKYVLDYIRSKGMIP 384 L AA +G E F +D GW+ G+ G+G W P+ +RFP G+K + DY+ + GM Sbjct: 327 ALARRAAAMGVELFVVDDGWFGARTGDR-AGLGDWTPNPDRFPAGLKPLADYVHALGMQF 385 Query: 385 GLWLELEVMGINSPKLTETDDSWFFMRHGK-RVKDRSRYQLDYRNPKVIAHADGVISRLV 443 G+W+E E++ +S +L W + G+ R + R++ L+ V + L+ Sbjct: 386 GIWVEPEMVNPDS-ELYRAHPDWVQFQPGRTRTEFRNQLVLNLAREDVQQCLWEQLDGLL 444 Query: 444 EQYGVGYIKMDYNINAGIGTETDADSFGDGLLQ-----HNRAYLAWLDSIFARYPNLVIE 498 V Y+K D+N TDA G+ Q H RA LD + A +P + E Sbjct: 445 SSAPVDYVKWDFNR-----CFTDAGWPGEAYPQRLWVDHVRALYRLLDRLRAAHPGVAFE 499 Query: 499 NCSSGGMRMDYAMLSRHSIQSTSDQEDYVQYAAIAAGSPAALTPEQSAVW--SYPLRE-- 554 +CS GG R+D +++R TSD D + AI G A W P + Sbjct: 500 SCSGGGGRIDLGIMARTDQVWTSDNTDPLDRLAIQHGFSQIHPARVMAAWVTDSPNTQLN 559 Query: 555 GDDEEVIFNMVNALLLRVHQSGHLAELEPRRRELVKEALDYYKSIRAHIPQATAF 609 G + V+A+ + G LAE R ++ + YK IR + + + Sbjct: 560 GRVSTLRLRFVSAMAGVLGVGGDLAEWSERELVEARDWVSLYKEIRPVVQRGDLY 614 >ref|NP_561290.1| alpha-galactosidase [Clostridium perfringens] dbj|BAB80080.1| alpha-galactosidase [Clostridium perfringens str. 13] Length = 735 Score = 107 bits (267), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 108/433 (24%), Positives = 184/433 (42%), Gaps = 33/433 (7%) Query: 203 SAAELLPMAVLH----NKESGTSLFWQIEHNGSWHWEL-TDQYDQLTLLVSGPTEHDNHW 257 S+AE P L ++ SG + + + ++G++ E+ D Y+Q T ++ G W Sbjct: 225 SSAEFNPFLALRRPNTDEFSGEVIGFSLVYSGNFMAEIDVDTYNQ-TRIMMGIHPERFSW 283 Query: 258 WLKLAPGEEFTSXXXXXXXXXXXXXXXXEQL-TAYRRMIRRPNEDNELLRIIFNDYMNCL 316 L L EEF + + + YR + R N + I+ N + L Sbjct: 284 PLNL--NEEFYTPEVVIVYSDKGLNYMSQVYHSLYRECLMRGKWKNSVRPILLNSW-EAL 340 Query: 317 WGSPTTGKLLPLIDAAADVGCEYFCIDAGWYAPGEWWD-GVGQWEPSAERFPEGIKYVLD 375 S K+ L A+ +G E F +D GW+ + G+G W + E+FP G+ +++ Sbjct: 341 SFSIDEEKIKELATNASKLGVELFVLDDGWFGKRNNDNAGLGDWTVNKEKFPNGLNEIIE 400 Query: 376 YIRSKGMIPGLWLELEVMGINSPKLTETDDSWFFMRHGKRVKDRSRYQLDYRNPKVIAHA 435 YI GM G+W+E E++ +S D + + K R++Y LD+ +V+ H Sbjct: 401 YINKLGMDFGIWIEPEMVNKDSELYRSHPDWIIYDPNRKPSHTRNQYTLDFSRDEVVDHI 460 Query: 436 DGVISRLVEQYGVGYIKMDYNINAGIGTETDADSFGDGLLQHNRAYLAW--LDSIFARYP 493 I +L+ Y + Y+K D N D + G + H + D + R+P Sbjct: 461 YNQIEKLLSYYNISYVKWDMNRYITECYSKDKGANLQGTVYHKYILNVYKLYDKLTTRFP 520 Query: 494 NLVIENCSSGGMRMDYAMLSRHSIQSTSDQEDYVQ-----------YAAIAAGSPAALTP 542 N++ E+CSSGG R D ML TSD D ++ Y I+ GS + +P Sbjct: 521 NILFESCSSGGARFDPGMLYYAPQTWTSDNTDAMERIKIQYGSSLVYPLISMGSHVSESP 580 Query: 543 EQSAVWSYPLREGDDEEVIFNMVNALLLRVHQSGHLAELEPRRRELVKEALDYYKSIRAH 602 Q L + N+ L L+++E +E +K+ + +YK R Sbjct: 581 NQQVFRETALETRANVAYFGNLGYEL-----DVNKLSDVE---KEEIKKQIQFYKENR-E 631 Query: 603 IPQATAFWPLGLP 615 + Q F+ + P Sbjct: 632 VFQFGEFYRIKNP 644 >gb|AAF68953.1| alpha-galactosidase [Absidia corymbifera] Length = 729 Score = 107 bits (266), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 146/597 (24%), Positives = 243/597 (40%), Gaps = 44/597 (7%) Query: 75 LRMVYGGHSDSVNALGRKLEITLTD-PVTGITAVQHLQFYTGVQTVRCWTVLKNTGTGEA 133 L ++Y G + L L+I L+D T H + T +R ++N G Sbjct: 95 LNLLYSGAKSKDDTL---LDIELSDGNRTDFKVTLHYELDTENDMIRRSYTVENGLKGRV 151 Query: 134 AVEYVSTFALTGV-DKEGSGDRNDKIEVTIAHSGWQSELQWRTYRLPELGMSHLADRGSK 192 ++ AL+ + D ++K E+ W +E Q ++ L + G++H + K Sbjct: 152 NLD----LALSAAWHPPTALDVDEKRELLTLAGEWNNEAQVQSTEL-KPGIAHTI-QTPK 205 Query: 193 RIAASNTGSWSAAELLPMAVLHNKESGTSLFW-QIEHNGSWHWELTDQYDQLTLL-VSGP 250 + + A +P V GT +++ + GSW E+T + ++G Sbjct: 206 GFTDHVSYPYFAIRQVPSEV----NPGTEVYFGTLAWGGSW--EITAHTNTYGYTRITGG 259 Query: 251 TEHDNHWWLKLAPGEEFTSXXXXXXXXXXXXXXXXEQLT----AYRRMIRRPNEDNELLR 306 H + W L PGE FT+ +L Y+++ + +++ L Sbjct: 260 MHHLDFGW-TLEPGESFTTPVFIAGFTDEGLPGARRRLPRHARKYQQLSLQTQKNDSLYH 318 Query: 307 IIFNDYMNCLWGSPTTGKLLPLIDAAADVGCEYFCIDAGWYAP-GEWWDGVGQWEPSAER 365 + + + T K + L + AA +G E F ID GW+ G+G W P+ E+ Sbjct: 319 PVLYNSWEAVTFDVTFDKQVALAEKAAPLGVELFVIDDGWFGDRNNDSAGLGDWYPNKEK 378 Query: 366 FPEGIKYVLDYIRSKGMIPGLWLELEVMGINSPKLTETDDSWFFMRHGKRVKDRSRYQLD 425 FP G+K + D++ GM G+W E E + NS E D + R + R++ L+ Sbjct: 379 FPNGLKPLADHVHDLGMQFGVWFEPESVNPNSNLYREHPDWVLYYDGVPRYEARNQLLLN 438 Query: 426 YRNPKVIAHADGVISRLVEQYGVGYIKMDYN-INAGIGTETDADSFGDGLLQHNRAYLAW 484 P+V + +S ++E+ + YIK D N G+ + + + R Y Sbjct: 439 LGLPEVQDYIYDRVSSIIEENDIDYIKWDMNRPYQGVTMHHYDRNPREAWVLIARGYQNL 498 Query: 485 LDSIFARYPNLVIENCSSGGMRMDYAMLSRHSIQSTSDQEDYVQYAAIAAGSPAALTPEQ 544 L + R+PNL IE+C+SGG RMD ++L TSD I G+ L P Sbjct: 499 LAKLKKRFPNLWIESCASGGGRMDLSVLEHADQVWTSDNTRPDARLKIQYGASLFLPPR- 557 Query: 545 SAVWSYPLREGDDEEVIFNMVNALLLRVHQS-----GHLAELEPRRRELVKEA---LDYY 596 A++ + GDD V L R H S G A L +KE+ + Y Sbjct: 558 -AMYGWVTESGDDNNVHI----PLSFRFHTSFMGGLGIGANLNKYSDNDMKESTAWIALY 612 Query: 597 KSIRAHIPQATAFWPLGLPDSEGEWVSFGLRHGDTRYLAV---WRIAGEAAAVKLPI 650 K +R I W L P GE ++ + AV +RI+ +A P+ Sbjct: 613 KKLRPVIQNGDLDW-LVPPSKVGEMIAVSQTTSKDQQEAVILAFRISSPFSAPMNPV 668 >ref|NP_822257.1| putative alpha-galactosidase [Streptomyces avermitilis MA-4680] dbj|BAC68792.1| putative alpha-galactosidase [Streptomyces avermitilis MA-4680] Length = 710 Score = 103 bits (258), Expect = 1e-20, Method: Compositional matrix adjust. Identities = 111/425 (26%), Positives = 176/425 (41%), Gaps = 32/425 (7%) Query: 109 HLQFYTGVQTVRCWTVLKNTGTGEAAVEYVSTFALTGVDKEGSGDRNDKIEVTIAH--SG 166 H + + WTV++N G + + A T + ++H Sbjct: 129 HYRVRADTDVIERWTVVRNGGDEPVTLLRADSAAWT---------LPPLADYRLSHVTGQ 179 Query: 167 WQSELQWRTYRLPELGMSHLADRGSKRIAASNTGSWSAAELLPMAVLHNKESGTSLFWQI 226 W +E + R RLP G + L R + I + +T W + H + +L W Sbjct: 180 WSAESRLRRDRLP-YGETVLTSR--RGITSHHTNPWLMVDSGEATEDHGRVWSAALAW-- 234 Query: 227 EHNGSWHWELTDQYDQLTLLVSGPTEHDNHWWLKLAPGEEFTSXXXXXXXXXXXXXXXXE 286 +GSW D T G HD + L PGEEFT+ Sbjct: 235 --SGSWRITTQRTPDGRTGFTGG-VGHDGTG-VPLTPGEEFTAPAFAGLFTADGFGAASR 290 Query: 287 QLTAYRRMIRRPNEDNELLRIIFNDYMNCLWGSPTTGKLLPLIDAAADVGCEYFCIDAGW 346 AY P+ D E + +++N + + G+ L + AA +G E + +D GW Sbjct: 291 AWHAYTLAHVLPHPD-EPVPVLYNSWEATGFDVDEVGQKA-LAERAAALGVELYVVDDGW 348 Query: 347 YAPGEWWD--GVGQWEPSAERFPEGIKYVLDYIRSKGMIPGLWLELEVMGINSPKLTETD 404 + G D G+G W P+ +RFP+G+ + D +R GM GLW+E E++ +S Sbjct: 349 FG-GRRSDRAGLGDWTPAPDRFPDGLGPLADRVRELGMRFGLWVEPEMVNPDSDLYRAHP 407 Query: 405 DSWFFMRHGKRVKDRSRYQLDYRNPKVIAHADGVISRLVEQYGVGYIKMDYN---INAG- 460 D + R + R++ L++ V A G ++ LV + + ++K D N AG Sbjct: 408 DWVLHLPDRSRSELRNQLVLNFARRDVADWAYGWLTELVGAHRIDFLKWDMNRAFSEAGW 467 Query: 461 IGTETDADSFGDGLLQHNRAYLAWLDSIFARYPNLVIENCSSGGMRMDYAMLSRHSIQST 520 G + AD G Q+ R +D + A +P L IE+CS GG R+D +L R Sbjct: 468 PGQQDGADRLG---TQYVRHLYGVIDRLRADHPGLRIESCSGGGGRIDLGILGRTDQTWA 524 Query: 521 SDQED 525 SD D Sbjct: 525 SDNTD 529 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,105,319,157 Number of extensions: 48201182 Number of successful extensions: 104299 Number of sequences better than 1.0e-05: 56 Number of HSP's gapped: 104217 Number of HSP's successfully gapped: 56 Length of query: 698 Length of database: 757,237,578 Length adjustment: 133 Effective length of query: 565 Effective length of database: 396,129,411 Effective search space: 223813117215 Effective search space used: 223813117215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 130 (54.7 bits)