BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0027.p (446 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAB00842.1| maltose-binding protein [Thermoanaerobacterium th... 108 2e-22 ref|ZP_00121355.1| COG1653: ABC-type sugar transport system, per... 93 1e-17 ref|ZP_00121052.1| COG1653: ABC-type sugar transport system, per... 76 2e-12 ref|NP_622452.1| Sugar-binding periplasmic proteins/domains [The... 74 8e-12 dbj|BAB97301.1| putative sugar-binding protein [Thermoanaerobact... 71 6e-11 >gb|AAB00842.1| maltose-binding protein [Thermoanaerobacterium thermosulfurigenes] Length = 446 Score = 108 bits (270), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 116/410 (28%), Positives = 180/410 (43%), Gaps = 39/410 (9%) Query: 51 STKPVTINMFTASPEYTDAFNAYIAEYKKVKPNVTINLEIMQA--DYNTVLKSKIAAGST 108 +++PVT+++F E DA I Y K PNV INLE + DY LK+K +GS Sbjct: 49 TSQPVTLDIFQFKVEIKDALQNAINTYMKENPNVKINLETVGGGQDYGAALKAKFNSGSE 108 Query: 109 PDVFQTTAGGDIDTFAEYSADLTNEPLASAMTDAVRSNMTSSDGKVLGLPVKGNLFVLMY 168 P ++ D+ T+ ADL++ A A + +T DGK+ GLP + L+Y Sbjct: 109 PAIYNVGGPQDVATWKPKLADLSDTKAAKAALPGTLTGVT-QDGKIYGLPFDLEGYGLIY 167 Query: 169 NKKLLADAGI-TNVPKTTAELDDAITKLEAK----GITP-FANAYKEWWVWK-HIFQHFV 221 NK++ + AGI + KT L +A+ L++K GI FA KE WV H+ F+ Sbjct: 168 NKQIFSKAGIDPSSIKTFDALVNAVKTLDSKKKELGIEAVFAFPAKETWVTGLHLSNAFL 227 Query: 222 ----DAAATDAGIDAKTLVGNFIAGDTTFKDHPVLYNNFFNFIDTTIKHGTDKPLERDSN 277 D A ++KT+ +F GD + ID K+ D + Sbjct: 228 SPEFDGDVMKA-FNSKTV--DFKYGDAMKQ-----------MIDLQNKYSVQPTASLDYS 273 Query: 278 AEVSD-FALGKAAFMTGKGAWDEEAIKKITPDF---DLGIAGYPVSDKPEQSQIITGADQ 333 +V F GK A M +G W ++ + +F D+G+ YPV E S G Sbjct: 274 TQVEKLFGTGKVA-MIQQGNWVYPTLESLDKNFAQNDIGMLPYPVPGYKEDS-YPEGVPM 331 Query: 334 ALRINKDSAVAAQTI--EFFNWLYTSEYGKNWFSTVAKVIPPIKDAPMPDLQMPKEMEEI 391 +N D Q +F +WLYTS+ GK + K +P + P M+ Sbjct: 332 YWAVNSDKPADVQKAAKDFLDWLYTSDEGKQIVTQQFKFVPAYTGYNSNSISDPLGMQLF 391 Query: 392 LKTEKS---GDLSVNYSLDTFHQKFGELMQAYIGGSKTKDQAIDEIQKAW 438 + + G + + Y + G +Q Y+ GS + D+ I ++AW Sbjct: 392 VAASQGKTYGWVFMGYPNNWGQNVLGADIQLYVNGSLSWDKVISHAKQAW 441 >ref|ZP_00121355.1| COG1653: ABC-type sugar transport system, periplasmic component [Bifidobacterium longum DJO10A] Length = 470 Score = 93.2 bits (230), Expect = 1e-17, Method: Compositional matrix adjust. Identities = 116/416 (27%), Positives = 180/416 (43%), Gaps = 55/416 (13%) Query: 54 PVTINMFTASPEYTDA-FNAYIAEYKKVKPNVTINL--EIMQADYNTVLKSKIAAGSTPD 110 PV + +T P TDA + + A ++K PN+ IN ADY L++ +A G PD Sbjct: 70 PVELTFWTWQP--TDAQWKSIYAAFQKKYPNIKINWWRTSEMADYQKKLQTSMAGGEGPD 127 Query: 111 VFQTTAGGDIDTFAEYSADLTNEPLA--------SAMTDAVRSNMTSSDGKVLGLPV--K 160 VF AG ++ + ++ D+ + LA S + + + T+ DGK++ +P+ Sbjct: 128 VFGVQAGSTVEQYGRFADDM--KELADKYMSGWDSKVAEGAVAQTTNKDGKMVAMPIITS 185 Query: 161 GNLFVLMYNKKLLADAGITNVPKTTAELDDAITKLEAKGITPFANAYKEWWVWKHIFQHF 220 G+ ++L YNK LL + GI VP T EL A +L+AKG+ P A K+ W IF Sbjct: 186 GSEYIL-YNKTLLDEQGI-KVPTTYDELVAANKELKAKGLMPLALGAKDGWHLDDIFVWL 243 Query: 221 VDAAATDAGIDAKTLVGNFIAGDTTFKDHPVLYNNFFN---FIDTTIKHGTDKPLERDSN 277 + G K G D TF + + F D + T P RD+ Sbjct: 244 SNQYGE--GDVYKAAEGKAKFTDETFVKTMKAWKQMIDDGLFQDGAVGTAT-YPDARDNY 300 Query: 278 AEVSDFALGKAAFMTGKGAW--------DEEAIKKITPDFDLGIAGYP-VSDKPEQSQII 328 F K+AF G+W DE + D +LG+ +P V DK + Sbjct: 301 -----FYARKSAFFP-TGSWHVSAVLPNDETKGTAVEKD-ELGMMEFPQVGDKA--TGPT 351 Query: 329 TGADQALRINKDSAVAAQTIEFFNWLYTSEYGKNWFSTVAKVIPPIKDAPMPDLQMPKEM 388 TG D L +NKDS ++F ++ T E + W +T+ P KD +++P + Sbjct: 352 TGVDFGLSVNKDSKKKEAAMKFIEFMTTGEGQQEWINTLQGA-PVDKDMK---VEVPSDA 407 Query: 389 EE--------ILKTEKSGDLSVNYSLDTFHQKFGELMQAYIGGSKTKDQAIDEIQK 436 E + K + S L + + + G MQ G T D A+ IQ+ Sbjct: 408 TESAKASIDLVAKGQASSKLERKLTSQELNDEIGVQMQNIYTGDATVDSALKAIQQ 463 >ref|ZP_00121052.1| COG1653: ABC-type sugar transport system, periplasmic component [Bifidobacterium longum DJO10A] Length = 421 Score = 75.9 bits (185), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 79/311 (25%), Positives = 132/311 (42%), Gaps = 30/311 (9%) Query: 74 IAEYKKVKPNVTINLEIMQ--ADYNTVLKSKIAAGSTPDVFQTTAGGDIDTFAEYSA-DL 130 + ++K P++T++++ Q A Y + L++++A G P +F T D +A D+ Sbjct: 56 VKGFEKKNPDITLDVQYGQDPAQYISTLQTRLAGGKAPTIFNLTMDNRTDVMKSGAALDI 115 Query: 131 TNEPLASAMTDAVRSNMTSSDGKVLGLPVKGNLFVLMYNKKLLADAGITNVPKTTAELDD 190 + E + D + DGK G+PV + V YNK +L AG PKT E + Sbjct: 116 SGEDFLYGIDD-TNFALFQQDGKTYGMPVSAWVGVFFYNKDILKKAGCDKFPKTWDEFIE 174 Query: 191 AITKLEAKGITPFANAYKEWWVWKHIFQHFVDAAATDAGIDAKT--LVGNFIAGDTTF-K 247 K+ + G T F + I F A+ G K+ L + +G +TF K Sbjct: 175 MGKKINSNGSTAFLEDFNT-----QIAGSFTGLLASYYGEQGKSGDLDADIWSGKSTFTK 229 Query: 248 DHPVLYNNFFNFIDTTIKHGT--DKPLERDSNAEVSDFALGKAAFMTGKGAWDEEAIKKI 305 D ++ + + K G K + ++ +F G M G WD ++K Sbjct: 230 DWTPVFKKW----EAAAKAGVIPQKSVGLSADQIKQEFVSGNLGVMR-SGPWDLPDLQK- 283 Query: 306 TPDFDLGIAGYPVSDKPEQSQIITGADQALRI-----NKDSAVAAQTIEFFNWLYTSEYG 360 D D G+A +P K + I G DQ I +K+ A A + + + N SE G Sbjct: 284 -SDIDFGVAPFPAYSKEDGQWINGGPDQGFAIASKASDKEQAAAKKFLAYLN----SEEG 338 Query: 361 KNWFSTVAKVI 371 F++ A + Sbjct: 339 LEAFTSAAGTL 349 >ref|NP_622452.1| Sugar-binding periplasmic proteins/domains [Thermoanaerobacter tengcongensis] gb|AAM24056.1| Sugar-binding periplasmic proteins/domains [Thermoanaerobacter tengcongensis] Length = 417 Score = 73.6 bits (179), Expect = 8e-12, Method: Compositional matrix adjust. Identities = 52/166 (31%), Positives = 86/166 (51%), Gaps = 23/166 (13%) Query: 50 DSTKPVTIN--MFTASPEYTDAFNAYIAEYKKVKPNVTINLEIMQADYNTVLKSKIAAGS 107 +++KPVTI M+++SP + IA++K+ PN+ + +E + DY L++++A+ + Sbjct: 37 ETSKPVTIKLGMWSSSPAEKKIVDDQIAKFKEKYPNIDVQIETIVGDYMQKLQTELASNT 96 Query: 108 TPDVF--------QTTAGGDIDTFAEY----SADLTN-EPLASAMTDAVRSNMTSSDGKV 154 PD+F Q + G ++ EY + D+ + EP A+ A + +GK Sbjct: 97 APDIFYLDSMPAPQLMSSGVLEPLDEYIKKYNVDVNDFEP---ALLSAFQW-----EGKT 148 Query: 155 LGLPVKGNLFVLMYNKKLLADAGITNVPKTTAELDDAITKLEAKGI 200 GLP N VL YNK + +AGI PKT EL + KL G+ Sbjct: 149 YGLPKDYNTLVLFYNKDMFKEAGINEPPKTWEELRETAKKLTKNGV 194 >dbj|BAB97301.1| putative sugar-binding protein [Thermoanaerobacter brockii] Length = 239 Score = 70.9 bits (172), Expect = 6e-11, Method: Compositional matrix adjust. Identities = 52/166 (31%), Positives = 83/166 (50%), Gaps = 23/166 (13%) Query: 50 DSTKPVTIN--MFTASPEYTDAFNAYIAEYKKVKPNVTINLEIMQADYNTVLKSKIAAGS 107 ++ KPVTI M+++SP + IA++K+ PN+ + +E + DY L++++A+ + Sbjct: 42 ETAKPVTIKLGMWSSSPAEKKIVDDQIAKFKEKYPNIDVQIETIVGDYMQKLQTELASNT 101 Query: 108 TPDVF--------QTTAGGDI----DTFAEYSADLTN-EPLASAMTDAVRSNMTSSDGKV 154 PD+F Q + G + D +Y+ D+ + EP A+ A + +GK Sbjct: 102 APDIFYLDSMPAPQLMSSGVLEPLDDYIKKYNVDVNDFEP---ALLSAFQW-----EGKT 153 Query: 155 LGLPVKGNLFVLMYNKKLLADAGITNVPKTTAELDDAITKLEAKGI 200 GLP N L YNK + AGI PKT EL D KL G+ Sbjct: 154 YGLPKDFNTLALFYNKDMFKAAGINEPPKTWEELRDVAKKLTKDGV 199 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.131 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 604,030,904 Number of extensions: 24978926 Number of successful extensions: 69419 Number of sequences better than 1.0e-05: 49 Number of HSP's gapped: 69468 Number of HSP's successfully gapped: 49 Length of query: 446 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 317 Effective length of database: 406,989,807 Effective search space: 129015768819 Effective search space used: 129015768819 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 127 (53.5 bits)