BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0026.p (548 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_563003.1| two-component response regulator [Clostridium p... 155 2e-36 ref|NP_244313.1| BH3446~unknown conserved protein [Bacillus halo... 104 5e-21 ref|NP_269623.1| putative two-component response regulator [Stre... 98 5e-19 ref|NP_241989.1| BH1123~unknown conserved protein [Bacillus halo... 98 7e-19 ref|NP_607636.1| putative two-component response regulator [Stre... 98 7e-19 >ref|NP_563003.1| two-component response regulator [Clostridium perfringens] dbj|BAB81793.1| two-component response regulator [Clostridium perfringens str. 13] Length = 507 Score = 155 bits (393), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 133/547 (24%), Positives = 266/547 (48%), Gaps = 52/547 (9%) Query: 1 MYKVLIIDDEEPLREAIHILGDWKGLGVDQVLEATDGRVGLEMLRKHKIDLALVDMKMPE 60 MYKV++ DDE + + + + +W+ LG++ V +A++G+ ++ +++ +D+ + D+ MP+ Sbjct: 1 MYKVMLADDENLILQGLENIIEWEELGLEIVNKASNGQEAIDKFKENPVDIVVTDINMPQ 60 Query: 61 LNGSQLLQIAEREFPDLLLIVISGYNDFEYTRQAIRSKVVDYLLKPVNRSDLNSALRKAM 120 + G +LL+ ++ D+ I++SGY+DF Y ++AI V +Y+LKP++ +L L+ + Sbjct: 61 VTGLELLKELKKINSDVKFIILSGYDDFSYAKKAIELGVENYILKPIDEEELEKTLKNTI 120 Query: 121 D-VLEAKRKRESEFITRNITLNMSLPKLKEKMYLSIIERSFKNQSNEAFLPLIGADKPGN 179 + + + K + +S NI + + +K K+ IE ++ E F + +++ Sbjct: 121 NKIKQEKEENKSSLGKHNILIKL----IKGKLAQGEIE-----ENKEGFYMNLNSER--- 168 Query: 180 HFAVGVLRMLNVEQVRHARFHEDRDLLHFAVTNVINENSGGHFEAFSFASPRSEREFIAI 239 +++ ++ +R+ + ++LH + NVI EN+ +FE + E I I Sbjct: 169 -YSLCIINT-------RSRY-DSEEMLH-NIVNVIKENTQNNFEIIYTL----DEELILI 214 Query: 240 FTLKGSYGEDAAFLSLHHMKKTVSTLKEL----FGILCAGGLGQPYSEVMGIAQSYELAK 295 S+ E LS +KK LKE +GI +G+P ++ I+ SY+ A Sbjct: 215 ----NSWDE---ALSKKEIKKYYDKLKEQIINEYGIDVFLSVGEPVCDLYKISSSYKEAN 267 Query: 296 AALDGTDLLTLKGSTVSNSGYTKSAAKDSPSLTGRMPLIRSSLESGNAGHAKSILSDFT- 354 +LTL + + K + + + + + + + + + A+ I+ + Sbjct: 268 NL--KKYVLTLGYNKCITTEDVKDINEKNINFSEVLDKLNKRIIAKDIEGAEKIIEETVE 325 Query: 355 -RKWKSSEHFSLGEADRIIQEFIILLGDIAVELDAVPERLRSGKSKGLRGLGISSDFASF 413 +K + L + + LL I E E+ +G S G + + S+ Sbjct: 326 DKKLNPRNIYDLS------VKILFLLDGIVEEFKV--EKQYTGNSLGEEIVALCSEDTRE 377 Query: 414 GEFEAVLSSILDVYAGEISRSLAGDRSSVLENIKAYIDNHYFENIKISMFTDKYFLSREY 473 + S I +V E+ S V++ I +Y++ +Y+E + + KY ++ Y Sbjct: 378 DIKTLLCSEIREVI--ELMHPTTIKYSPVIQQIISYVNENYYEEVSLKTLAQKYHINTSY 435 Query: 474 LMKLFKGQYGYGIHEYVQKVRMDKAKELLSDPALKIQDISERLGYKDKNYFSKAFRNYYD 533 L ++F + G EY+ K + KAKEL+ + +KI DI++++GY D +YF + F+ YY Sbjct: 436 LGQVFTKEVGCSFSEYLNKTKNMKAKELILNTNMKINDIAKKVGYLDTSYFYRKFKKYYG 495 Query: 534 CSPSEFR 540 PS R Sbjct: 496 VCPSTLR 502 >ref|NP_244313.1| BH3446~unknown conserved protein [Bacillus halodurans] pir||F84080 hypothetical protein BH3446 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07165.1| BH3446~unknown conserved protein [Bacillus halodurans] Length = 200 Score = 104 bits (260), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 64/191 (33%), Positives = 106/191 (55%), Gaps = 5/191 (2%) Query: 2 YKVLIIDDEEPLREAIHILGDWKGLGVDQVLEATDGRVGLEMLRKHKIDLALVDMKMPEL 61 + VLI DDE +RE I +W L + V EA DG LE+ +H++D+ L D+ MP + Sbjct: 4 WNVLIADDEIIIREGIRDAINWSELQLTVVAEAEDGEEALELALEHQVDILLADLNMPIM 63 Query: 62 NGSQLLQIAEREFPDLLLIVISGYNDFEYTRQAIRSKVVDYLLKPVNRSDLNSALRK-AM 120 NG L++ P +I+I+G+++F Y ++AIR VVDY++KPV+ L L+K + Sbjct: 64 NGLTLIKQLRTALPHCKVIIITGHDEFVYAQEAIRLSVVDYIIKPVDADQLYEVLKKVCI 123 Query: 121 DVLEAK-RKRESEFITRNITLNMSLPKLKEKMYLSIIERSFKNQSNEAFLPLIGADKPGN 179 ++ E + RKRE F + + N++L L+E+ + + + N+ L + Sbjct: 124 ELTEGQNRKREETFTAKQLEKNITL--LREQFCSNWVMGALTNEEVAEQLSFLQLPDTLP 181 Query: 180 HFAVGVLRMLN 190 F +GV+R LN Sbjct: 182 SF-IGVIRCLN 191 >ref|NP_269623.1| putative two-component response regulator [Streptococcus pyogenes] gb|AAK34344.1| putative two-component response regulator [Streptococcus pyogenes M1 GAS] Length = 246 Score = 98.2 bits (243), Expect = 5e-19, Method: Compositional matrix adjust. Identities = 48/131 (36%), Positives = 81/131 (61%) Query: 1 MYKVLIIDDEEPLREAIHILGDWKGLGVDQVLEATDGRVGLEMLRKHKIDLALVDMKMPE 60 MY +LI++DE +R+ I L D+ +D+V EA +G++ ++ +K D+ L D+ MP+ Sbjct: 1 MYSLLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLFQKEPYDIVLTDINMPK 60 Query: 61 LNGSQLLQIAEREFPDLLLIVISGYNDFEYTRQAIRSKVVDYLLKPVNRSDLNSALRKAM 120 LNG QL ++ ++E P L+ ++GY+DF Y A++ DYLLKP +++D+ L K Sbjct: 61 LNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDYLLKPFSKADVEDMLGKLR 120 Query: 121 DVLEAKRKRES 131 LE +K E+ Sbjct: 121 KKLELSKKTET 131 >ref|NP_241989.1| BH1123~unknown conserved protein [Bacillus halodurans] pir||C83790 hypothetical protein BH1123 [imported] - Bacillus halodurans (strain C-125) dbj|BAB04842.1| BH1123~unknown conserved protein [Bacillus halodurans] Length = 526 Score = 97.8 bits (242), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 55/186 (29%), Positives = 103/186 (55%), Gaps = 1/186 (0%) Query: 1 MYKVLIIDDEEPLREAIHILGDWKGLGVDQVLEATDGRVGLEMLRKHKIDLALVDMKMPE 60 M+KV+IIDDEE + + + WK LG + V EA +GR GL+++R+ + L D+ MP Sbjct: 1 MWKVVIIDDEEHVVRGLKKIISWKALGCECVGEAANGRRGLDIVRETSPHIVLTDIYMPI 60 Query: 61 LNGSQLLQIAEREFPDLLLIVISGYNDFEYTRQAIRSKVVDYLLKPVNRSDLNSALRKAM 120 + G ++QI + D ++++SGY++FE+ R+A++ + DYL KP + + + L++ + Sbjct: 61 MYGLDMIQILREDGFDGEVVILSGYSEFEHARKAMQLNITDYLSKPASVDTIETVLKRVI 120 Query: 121 DVLEAKRKRESEFITRNITLNMSLPKLKEKMYLSIIERSFKN-QSNEAFLPLIGADKPGN 179 + LE K++++ + P ++ + S++ +N + E FL I K Sbjct: 121 NKLEEKQRQQGYLQELTNKVKRYEPLVETEWMKSLLTGRLRNGEIPEEFLAKITKWKDQQ 180 Query: 180 HFAVGV 185 H A + Sbjct: 181 HIACSL 186 Score = 70.9 bits (172), Expect = 8e-11, Method: Compositional matrix adjust. Identities = 32/99 (32%), Positives = 58/99 (58%) Query: 441 SVLENIKAYIDNHYFENIKISMFTDKYFLSREYLMKLFKGQYGYGIHEYVQKVRMDKAKE 500 ++E + YI+ H ENI + D+ ++SR YL ++FK G YV ++RM+KAK+ Sbjct: 424 QLIEQVMIYIEKHLAENITLQDIADELYISRNYLGQIFKKVVGESFKNYVTRLRMEKAKK 483 Query: 501 LLSDPALKIQDISERLGYKDKNYFSKAFRNYYDCSPSEF 539 ++ + I ++SE++GY + YF+ F+ Y +P + Sbjct: 484 MIQEGNFLIYEVSEKVGYVNPAYFTTMFKKYTGYTPRDL 522 >ref|NP_607636.1| putative two-component response regulator [Streptococcus pyogenes MGAS8232] gb|AAL98135.1| putative two-component response regulator [Streptococcus pyogenes MGAS8232] Length = 246 Score = 97.8 bits (242), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 47/131 (35%), Positives = 81/131 (61%) Query: 1 MYKVLIIDDEEPLREAIHILGDWKGLGVDQVLEATDGRVGLEMLRKHKIDLALVDMKMPE 60 MY +LI++DE +R+ + L D+ +D+V EA +G++ ++ +K D+ L D+ MP+ Sbjct: 1 MYSLLIVEDEYLIRQGVRSLVDFSQFKIDRVNEAENGQLAWDLFQKEPYDIVLTDINMPK 60 Query: 61 LNGSQLLQIAEREFPDLLLIVISGYNDFEYTRQAIRSKVVDYLLKPVNRSDLNSALRKAM 120 LNG QL ++ ++E P L+ ++GY+DF Y A++ DYLLKP +++D+ L K Sbjct: 61 LNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDYLLKPFSKADVEDMLGKLR 120 Query: 121 DVLEAKRKRES 131 LE +K E+ Sbjct: 121 KKLELSKKTET 131 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.136 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 773,955,542 Number of extensions: 31842309 Number of successful extensions: 100808 Number of sequences better than 1.0e-05: 1191 Number of HSP's gapped: 102766 Number of HSP's successfully gapped: 1227 Length of query: 548 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 417 Effective length of database: 401,559,609 Effective search space: 167450356953 Effective search space used: 167450356953 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)