BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0016.p (578 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongen... 185 2e-45 ref|NP_607635.1| putative histidine kinase [Streptococcus pyogen... 160 6e-38 ref|NP_561490.1| two-component sensor histidine kinase [Clostrid... 158 4e-37 ref|NP_782429.1| two-component sensor kinase yesM [Clostridium t... 157 5e-37 ref|NP_665005.1| putative two-component sensor histidine kinase ... 156 1e-36 >ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongensis] gb|AAM25485.1| predicted ATPase [Thermoanaerobacter tengcongensis] Length = 593 Score = 185 bits (470), Expect = 2e-45, Method: Compositional matrix adjust. Identities = 113/323 (34%), Positives = 180/323 (55%), Gaps = 18/323 (5%) Query: 258 IPGSSFVIESLVPDTYLTKDAGRLGRVIFALCALSFLVMTVI-GYVVARLSGRKMS---- 312 IP S + + S+V K+A R+ F L LV ++ ++V+ R ++ Sbjct: 274 IPTSDWRLVSVVS----LKNAINSIRISFIEFYLRILVFCLLFAFLVSMYFSRALTHPLE 329 Query: 313 RIVGLVRSFQEGNFQKRIRFS--GNDEFVQIADSFNVMATNIQELINSVYVQGIQKKQAE 370 R+ ++ ++GN ++++ F DE + +N M I +LIN + I K++AE Sbjct: 330 RLANSMKKVRQGNLEEKVEFEYHSKDEIAIVIKEYNRMVERINDLINRILKLEINKREAE 389 Query: 371 LEALQAQINPHFLYNTLSTISSLANLGEIEKVTGMVQGLSRFYRLTLNQGNVYIELEKEL 430 L+AL+AQINPHFLYNTL TI A + +++ M+ LSR +RLTLN+G +I++ E Sbjct: 390 LKALEAQINPHFLYNTLDTIFWKAEKAKDRELSEMIYSLSRLFRLTLNRGEEFIQVRGEK 449 Query: 431 EQVATYLDIQRVKYADAFTVYVDVEEEILHMQVIKLVLQPFVENVFKHAW--FGETIAIR 488 E + YL +Q +Y + F V + +EIL + KL+LQPFVEN H E + Sbjct: 450 ELIERYLFLQSKRYKNKFKYSVKISDEILDYYIPKLILQPFVENAIVHGLENNSEESFVE 509 Query: 489 LTGRRVGDNIELKVIDNGIGMRP---EDIKRMMQGPS--QAGGYGVKNVNERIKLRYGDD 543 + G + G+N++ + DNG GM E +KR ++ + + GY ++NVNER++L YGD Sbjct: 510 VIGEKEGENLKFIIKDNGPGMSEETLEKLKRFIESENIEEKVGYAIRNVNERLRLYYGDK 569 Query: 544 YGVTIASFFGAGTTVRLLLPAGM 566 Y + I S G GTT+ L+LP + Sbjct: 570 YELKIESRQGEGTTISLILPESI 592 >ref|NP_607635.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] gb|AAL98134.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] Length = 577 Score = 160 bits (406), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 109/330 (33%), Positives = 173/330 (52%), Gaps = 17/330 (5%) Query: 261 SSFVIESLVPDTYLT-------KDAGRLGRVIF----ALCALSFLVMTVIGYVVARLSGR 309 SSFV + ++P++ T R+ R IF + L+ L+ + +V R R Sbjct: 249 SSFVYQRVIPNSQWTLIGVASLDQLHRVQRQIFWSFLGMGLLACLICGLTTILVLRKWIR 308 Query: 310 KMSRIVGLVRSFQEGNFQKRIRFSGNDEFVQIADSFNVMATNIQELINSVYVQGIQKKQA 369 + ++ ++ + Q+GN Q R + +G+ E +A FN + I L+ +V + + Q Sbjct: 309 PIQQLQQVILAIQKGNRQLRAQETGSPELTDLAHQFNALLDQIDSLMVAVADKEKEIGQY 368 Query: 370 ELEALQAQINPHFLYNTLSTISSLANLGEIEKVTGMVQGLSRFYRLTLNQGNVYIELEKE 429 L+AL +QINPHFLYNTL TI +A + ++V + + L++++RL LNQGN YI L E Sbjct: 369 RLQALASQINPHFLYNTLDTIIWMAEFNDSKRVVEVTKSLAKYFRLALNQGNDYIRLADE 428 Query: 430 LEQVATYLDIQRVKYADAFTVYVDVEEEILHMQVIKLVLQPFVENVFKHAW--FGETIAI 487 L+ V+ YL IQ+ +Y D T V + + KL+LQP VEN H I Sbjct: 429 LDHVSQYLFIQKQRYGDKLTYDVQGLDAYADFIIPKLILQPLVENAIYHGIKEVDRKGMI 488 Query: 488 RLTGRRVGDNIELKVIDNGIGMRPEDIKRMMQGPSQAGGYGVKNVNERIKLRYGDDYGVT 547 ++T ++ L V DNG G+ + Q GG G+KNV++R+KL+YG+ Y +T Sbjct: 489 KVTVSETAQHLILTVWDNGKGIEASALTN-SQSLLTRGGVGLKNVDQRLKLQYGEAYQMT 547 Query: 548 IASFFGAGTTVRLLLPAG---MKDNEEMDE 574 I S T ++L LP M D+ + +E Sbjct: 548 IHSQSDHFTEIQLSLPKMHELMADDTQENE 577 >ref|NP_561490.1| two-component sensor histidine kinase [Clostridium perfringens] dbj|BAB80280.1| two-component sensor histidine kinase [Clostridium perfringens str. 13] Length = 529 Score = 158 bits (399), Expect = 4e-37, Method: Compositional matrix adjust. Identities = 110/416 (26%), Positives = 199/416 (47%), Gaps = 34/416 (8%) Query: 179 LDNLSHVRRLVNFSDYKTVIGYVRITVSLEDLFGGFDTFPV------------------E 220 L+N H+ + +++GYV + + E F F + E Sbjct: 120 LNNEPHICIVTPVKQDNSIVGYVFMAIKSEVFASSFSLFNIGKGSEIFIIDDKNNIIISE 179 Query: 221 EGITLRLVEETTGDILFQRGPANYDDA------GGDYLSLHEKIPGSSFVIESLVPDTYL 274 + + E T F + D A G +Y++ + K+ G ++ I S+ P ++ Sbjct: 180 NNLNSNFILEETDLNKFLKDKGAKDTARSIVVNGENYVATYRKLKGFNWYIASMTPYDFI 239 Query: 275 TKDAGRLGRVIFALCALSFLVMTVIGYVVARLSGRKMSRIVGLVRSFQEGNFQKRIRFSG 334 D L + + + L LV+ + Y + + ++ ++ EG+ + + + Sbjct: 240 NADIQSLRKEMTIIGLLFTLVVLIFSYFLYSSIKVPLYNLLNKMKRVSEGDLKVLVNDTY 299 Query: 335 NDEFVQIADSFNVMATNIQELINSVYVQGIQKKQAELEALQAQINPHFLYNTLSTISSLA 394 DE I+D+F+ M + ELI +Q ++K+ E+ LQAQINPHFL+NTL+++ A Sbjct: 300 EDEIGVISDNFDSMLKRVNELIEKTKLQEVEKRDLEIRMLQAQINPHFLFNTLNSLKWTA 359 Query: 395 NLGEIEKVTGMVQGLSRFYRLTLNQGNVYIELEKELEQVATYLDIQRVKYADAFTVYVDV 454 + + V+ + L+ R T+ N + + +ELE + Y+ IQ+++Y ++F V +V Sbjct: 360 LMNQDYTVSEGLSSLAELLRNTIIDKNELVTIREELENIKNYVVIQKIRYGNSFDVQYEV 419 Query: 455 EEEILHMQVIKLVLQPFVENVFKHAW----FGETIAIRLTGRRVGDNIELKVIDNGIGMR 510 +E +L +VIK +LQP VEN H + I I++ +NI + + DNG G Sbjct: 420 DELLLENKVIKFILQPIVENSIIHGIDENKENQKIIIKIMSEE--ENILISIKDNGKGFN 477 Query: 511 PEDIKRMMQGPSQAGGYGVKNVNERIKLRYGDDYGVTIASFFGAGTTVRLLLPAGM 566 ++ + ++ G G NV+ERIKL +G YG TI S GT V++++P + Sbjct: 478 SKEKVK----DNKLSGIGWNNVDERIKLTFGLGYGTTIYSEVNKGTIVKIVIPKSI 529 >ref|NP_782429.1| two-component sensor kinase yesM [Clostridium tetani E88] gb|AAO36366.1| two-component sensor kinase yesM [Clostridium tetani E88] Length = 577 Score = 157 bits (398), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 93/267 (34%), Positives = 141/267 (52%), Gaps = 19/267 (7%) Query: 309 RKMSRIVGLVRSFQEGNFQKRIRFSGNDEFVQIADSFNVMATNIQELINSVYVQGIQKKQ 368 + + R+ + +GNF NDE + FN M T+I+ LIN VY QK+ Sbjct: 308 KPIHRLKSNMLEISKGNFDSYYEIKNNDEISILGQVFNQMLTDIKRLINKVYQVEKQKRS 367 Query: 369 AELEALQAQINPHFLYNTLSTISSLANLGEIEKVTGMVQGLSRFYRLTLNQGNVYIELEK 428 AEL LQ+QINPHFLYNTL TI A +V M+ LS F+R++L+ G +I + Sbjct: 368 AELRVLQSQINPHFLYNTLDTIQWKALEYNAFEVVDMINYLSNFFRISLSDGKEFITISD 427 Query: 429 ELEQVATYLDIQRVKYADAFTVYVDVEEEILHMQVIKLVLQPFVENVFKHAWFGETIAIR 488 E+ V+ YL+IQ+++Y D + +DV+ + + KL++QP VEN H ++ Sbjct: 428 EVAHVSNYLEIQKIRYKDKISYSMDVDSSLDQYLIPKLIVQPLVENSIYH-------GLK 480 Query: 489 LTGRRVGDNIE---------LKVIDNGIGMRPEDIKRMMQG---PSQAGGYGVKNVNERI 536 L + N+ +KVIDNG+GM E ++ + + + YG+ N+NER+ Sbjct: 481 LQKHKGIINVSISSKDGFLFIKVIDNGLGMDVEQLRTLRKNLWESIETNHYGLYNINERL 540 Query: 537 KLRYGDDYGVTIASFFGAGTTVRLLLP 563 KL +G Y + I S GT V L +P Sbjct: 541 KLAFGKKYSIEIKSELKIGTQVLLRIP 567 >ref|NP_665005.1| putative two-component sensor histidine kinase [Streptococcus pyogenes MGAS315] ref|NP_801923.1| putative two-component sensor histidine kinase [Streptococcus pyogenes SSI-1] gb|AAM79808.1| putative two-component sensor histidine kinase [Streptococcus pyogenes MGAS315] dbj|BAC63756.1| putative two-component sensor histidine kinase [Streptococcus pyogenes SSI-1] Length = 577 Score = 156 bits (395), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 133/465 (28%), Positives = 213/465 (45%), Gaps = 43/465 (9%) Query: 122 TPNSQLNI-VSGDNLNEPIYDS--DYYILPLKDIADSGWYQSLKDSKRDNLWLQIDTDQK 178 + NSQL + S D + EP Y + D +P+ A S K+ W+ T + Sbjct: 119 STNSQLTMKTSSDMMAEPWYKAAIDCQAMPVLTPARQLSLSSKKE------WVVSVTQEV 172 Query: 179 LDNLSHVRRLVNFSDYKTVIGYVRITVSLEDLFGGFDTFPVEEGITLRLVEETTGDILFQ 238 +D H N + I Y I SL+ L G F + + ++ Sbjct: 173 VDAAGH-----NLGVLRLDIAYPTIKASLDQLQLGRQGFA--------FIVNDKHEFVYH 219 Query: 239 RGPANYDD-----AGGDYLSLHEKIPG--SSFVIESLVPDTYLT----KDAGRLGRV--- 284 + Y A YL++ +SFV + L+P++ T +L RV Sbjct: 220 PKKSVYSSSKEMAAMKPYLAIQNGYTKDKTSFVYQKLIPNSQWTLVGVASLDQLHRVQRQ 279 Query: 285 -IFALCALSFLVMTVIGYVVA---RLSGRKMSRIVGLVRSFQEGNFQKRIRFSGNDEFVQ 340 +++ L + G+ R R + ++ ++ + Q+G+ Q R + +G+ E Sbjct: 280 ILWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVILAIQKGDRQLRAQETGSPELTD 339 Query: 341 IADSFNVMATNIQELINSVYVQGIQKKQAELEALQAQINPHFLYNTLSTISSLANLGEIE 400 +A FN + I L+ +V + Q L+AL +QINPHFLYNTL TI +A + + Sbjct: 340 LAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQINPHFLYNTLDTIIWMAEFNDSK 399 Query: 401 KVTGMVQGLSRFYRLTLNQGNVYIELEKELEQVATYLDIQRVKYADAFTVYVDVEEEILH 460 +V + + L++++RL LNQGN YI L EL+ V+ YL IQ+ +Y D + V + Sbjct: 400 RVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFIQKQRYGDKLSYEVQGLDVYAD 459 Query: 461 MQVIKLVLQPFVENVFKHAW--FGETIAIRLTGRRVGDNIELKVIDNGIGMRPEDIKRMM 518 + KL+LQP VEN H I++T ++ L V DNG G+ + Sbjct: 460 FVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQHLMLTVWDNGKGIEDSSLTN-S 518 Query: 519 QGPSQAGGYGVKNVNERIKLRYGDDYGVTIASFFGAGTTVRLLLP 563 Q GG G+KNV++R+KL YG+ Y +TI S T ++L LP Sbjct: 519 QSLLARGGVGLKNVDQRLKLHYGEGYHMTIHSQSDQFTEIQLSLP 563 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.139 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 837,853,914 Number of extensions: 36384577 Number of successful extensions: 90776 Number of sequences better than 1.0e-05: 188 Number of HSP's gapped: 90545 Number of HSP's successfully gapped: 188 Length of query: 578 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 447 Effective length of database: 401,559,609 Effective search space: 179497145223 Effective search space used: 179497145223 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 129 (54.3 bits)