BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0015.p (338 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_636630.1| beta-galactosidase [Xanthomonas campestris pv. ... 272 6e-72 ref|ZP_00061173.1| COG3250: Beta-galactosidase/beta-glucuronidas... 264 3e-69 ref|NP_641643.1| beta-galactosidase [Xanthomonas axonopodis pv. ... 263 5e-69 ref|NP_641061.1| truncated beta-galactosidase [Xanthomonas axono... 258 1e-67 ref|ZP_00065376.1| COG3250: Beta-galactosidase/beta-glucuronidas... 257 2e-67 >ref|NP_636630.1| beta-galactosidase [Xanthomonas campestris pv. campestris str. ATCC 33913] gb|AAM40554.1| beta-galactosidase [Xanthomonas campestris pv. campestris str. ATCC 33913] Length = 926 Score = 272 bits (696), Expect = 6e-72, Method: Compositional matrix adjust. Identities = 141/313 (45%), Positives = 190/313 (60%), Gaps = 11/313 (3%) Query: 32 HEQHPGRLMIGSECASYTATRGIYENDPVRGYCSEYGTNIPSWGCTPQQAWSDVVNNRFL 91 H Q+P + GSE S + RG Y+ D RGY Y T+ P W T + WS V ++ Sbjct: 611 HAQYPNIPIYGSETGSTVSVRGNYQRDDTRGYTRAYDTDHPWWASTAEAWWSYVAQRPYI 670 Query: 92 TGVFMWTGFDYRGEPTPY-LWPCVNSHFGLMDTCGFPKDSYYYMQAVWKDEPIVHLLPHW 150 G F+WTGFDYRGEPTPY WP V S FG++D+CGFPKD+Y+Y +A W EP++HL PHW Sbjct: 671 AGGFIWTGFDYRGEPTPYNRWPNVGSQFGVLDSCGFPKDNYWYYRAQWTSEPVLHLFPHW 730 Query: 151 NW-----PGSEGKPVEVRVFANTETAELILNGSSLGEQQVDRTGYLTWEVHYEPGELKVI 205 NW P +G+ VEV +N E EL++NG S G QQV G++ W V Y PG ++ Sbjct: 731 NWDGLLQPDDKGR-VEVWCHSNLEAVELLVNGVSQGLQQVPAYGHVEWRVAYAPGVIEAR 789 Query: 206 GRNGGKEAAVKKAVTAGQPRQIRLYPDRAAGQADGMDTIPVRVAVLDELGHVVPTADHEI 265 G GGK ++ T G+P IRL DR+A +AD D V+V ++D G +VP ADH + Sbjct: 790 GYRGGKLVLTERRETVGKPAAIRLSCDRSALRADAEDVAVVKVEIVDAQGRLVPNADHLV 849 Query: 266 RFEVEGAGILLGVGNGNPSSHEPDKSPVRRAFNGWCLXXXXIQAAPEAGPVTLRAVSTGL 325 + G L+GVGNG+PSSHE DK+P R+AFNG C+ +++ +G + L+A + GL Sbjct: 850 HLVLRGPARLIGVGNGDPSSHEDDKAPQRKAFNGLCM--ALLRSTRSSGQILLQATAPGL 907 Query: 326 APAEVLLHFSAVT 338 A LH A T Sbjct: 908 TSAA--LHLQAAT 918 >ref|ZP_00061173.1| COG3250: Beta-galactosidase/beta-glucuronidase [Clostridium thermocellum ATCC 27405] Length = 941 Score = 264 bits (674), Expect = 3e-69, Method: Compositional matrix adjust. Identities = 131/329 (39%), Positives = 197/329 (59%), Gaps = 14/329 (4%) Query: 12 EVVDITGYNYGHRDHLDIRDHEQHPGRLMIGSECASYTATRGIYENDPVRGY-------C 64 V+D+ GYNY + + H++HP +M GSE +S +RG+Y+ + C Sbjct: 404 SVLDLVGYNY--FPFMYDQGHKEHPEWIMFGSETSSAVRSRGVYKTPTNQNILTGNDNQC 461 Query: 65 SEYGTNIPSWGCTPQQAWSDVVNNRFLTGVFMWTGFDYRGEPTPYLWPCVNSHFGLMDTC 124 S Y ++ +WG + + ++ ++ ++ G F+WTGFDY GEPTPY WP +S+FG++DTC Sbjct: 462 SSYDNSVVAWGNSAESSYYEINRRDYMLGEFVWTGFDYIGEPTPYKWPSKSSYFGIVDTC 521 Query: 125 GFPKDSYYYMQAVWKDEPIVHLLPHWNWPGSEGKPVEVRVFANTETAELILNGSSLGEQQ 184 GFPKD YY+ Q+ W D+P+VH+LPHWNW S G VEV ++N +T EL LNG+SLG + Sbjct: 522 GFPKDIYYFYQSKWSDKPMVHILPHWNW--SNGTTVEVWAYSNCDTVELFLNGTSLGVKS 579 Query: 185 VDRTGYLTWEVHYEPGELKVIGRNGGKEAAVKKAVTAGQPRQIRLYPDRAAGQADGMDTI 244 + G+++W V + PG L+ G + TAG P +I+L PDR ADG D + Sbjct: 580 MGNNGHVSWNVPWVPGTLRAKAVKGNI-VVYDEVTTAGNPAKIQLKPDRTTITADGKDLV 638 Query: 245 PVRVAVLDELGHVVPTADHEIRFEVEGAGILLGVGNGNPSSHEPDKSPVRRAFNGWCLXX 304 + ++D G +VPTA + + F + G G+++GV NGN +S EP K+ R+AFNG CL Sbjct: 639 FIETDIVDSNGVLVPTASNTVNFSISGPGVIVGVDNGNAASLEPYKANSRQAFNGKCL-- 696 Query: 305 XXIQAAPEAGPVTLRAVSTGLAPAEVLLH 333 +QA G + + A S GL V++ Sbjct: 697 VIVQATKTNGTIIVTASSNGLESDRVIIK 725 >ref|NP_641643.1| beta-galactosidase [Xanthomonas axonopodis pv. citri str. 306] gb|AAM36179.1| beta-galactosidase [Xanthomonas axonopodis pv. citri str. 306] Length = 854 Score = 263 bits (671), Expect = 5e-69, Method: Compositional matrix adjust. Identities = 135/306 (44%), Positives = 180/306 (58%), Gaps = 7/306 (2%) Query: 32 HEQHPGRLMIGSECASYTATRGIYENDPVRGYCSEYGTNIPSWGCTPQQAWSDVVNNRFL 91 H Q+P + GSE S + RG Y D RGY Y + P W T + WS V ++ Sbjct: 539 HAQYPNIPIYGSETGSTVSVRGNYRRDDQRGYTRAYDLDHPWWASTAEAWWSYVAQRPYI 598 Query: 92 TGVFMWTGFDYRGEPTPY-LWPCVNSHFGLMDTCGFPKDSYYYMQAVWKDEPIVHLLPHW 150 G F+WTGFDYRGEPTPY WP V S FG++D+CGFPKD+Y+Y +A W EP++HL PHW Sbjct: 599 AGGFIWTGFDYRGEPTPYNRWPNVASQFGVLDSCGFPKDNYWYYRAQWTSEPVLHLFPHW 658 Query: 151 NWPG----SEGKPVEVRVFANTETAELILNGSSLGEQQVDRTGYLTWEVHYEPGELKVIG 206 NW G + + V +N E EL++NG S G QQV G++ W V Y PG ++ G Sbjct: 659 NWDGLLEPDDNGRIAVWCHSNLEAVELLVNGVSQGLQQVPAYGHVEWRVVYAPGTIEARG 718 Query: 207 RNGGKEAAVKKAVTAGQPRQIRLYPDRAAGQADGMDTIPVRVAVLDELGHVVPTADHEIR 266 GGK ++ T G P IRL DR +AD D V+V +LD G +VPTAD ++ Sbjct: 719 YRGGKLVLSERRETTGNPAAIRLSCDRNTLRADAEDVAVVKVEILDAQGRLVPTADSLVQ 778 Query: 267 FEVEGAGILLGVGNGNPSSHEPDKSPVRRAFNGWCLXXXXIQAAPEAGPVTLRAVSTGLA 326 F + G L+GVGNG+PSSHE DK+P R+AFNG C +Q +G + L+A + GL Sbjct: 779 FALRGPARLIGVGNGDPSSHEDDKAPRRKAFNGLC--AALLQTTRSSGEIVLQATAPGLT 836 Query: 327 PAEVLL 332 + + L Sbjct: 837 SSTLRL 842 >ref|NP_641061.1| truncated beta-galactosidase [Xanthomonas axonopodis pv. citri str. 306] gb|AAM35597.1| truncated beta-galactosidase [Xanthomonas axonopodis pv. citri str. 306] Length = 593 Score = 258 bits (660), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 140/327 (42%), Positives = 188/327 (57%), Gaps = 7/327 (2%) Query: 1 MNHGWYENG-YNEVVDITGYNYGHRDHLDIRDHEQHPGRLMIGSECASYTATRGIYENDP 59 MN G + G +VVD+ G+NY R + R H P M+ SE S TRG + D Sbjct: 115 MNDGMFATGGAADVVDVLGFNY--RQYNYDRVHAARPRTPMLSSEDTSAFQTRGAWFTDL 172 Query: 60 VRGYCSEYGTNIPSWGCTPQQAWSDVVNNRFLTGVFMWTGFDYRGEPTPYLWPCVNSHFG 119 +E + WG T ++AW + F+ G F+WTGFDYRGEPTP+ WP V + FG Sbjct: 173 DAHVVAEDDSLAADWGNTHRRAWQLIAERPFVAGSFVWTGFDYRGEPTPFEWPSVGAFFG 232 Query: 120 LMDTCGFPKDSYYYMQAVWKDE-PIVHLLPHWNWPGSEGKPVEVRVFANTETAELILNGS 178 +MD CGF K +Y+ +A+W D+ P++HLLPHWNWPG EG+P++V F N E EL LNG Sbjct: 233 IMDQCGFAKGAYWLRRALWIDDAPVLHLLPHWNWPGREGQPIKVMAFCNAEQVELWLNGR 292 Query: 179 SLGEQQVDRTGYLTWEVHYEPGELKVIGRNGGKEAAVKKAVTAGQPRQIRLYPDRAAGQA 238 SLG+Q VDR W+V Y PG L+ + GG E A + T G P ++L PDR+ Sbjct: 293 SLGKQAVDRAQSNQWQVDYAPGVLEAVAWRGGHEVARTRVETTGAPVALKLTPDRSELHG 352 Query: 239 DGMDTIPVRVAVLDELGHVVPTADHEIRFEVEGAGILLGVGNGNPSSHEPDKSPVRRAFN 298 DG D PV + +D G VP + + ++ G G LLG+GNG+P+SH D+S FN Sbjct: 353 DGRDAQPVTLEAVDAQGRHVPDCNALLTLQISG-GRLLGIGNGDPNSHAADQSTQVALFN 411 Query: 299 GWCLXXXXIQAAPEAGPVTLRAVSTGL 325 G L +QA AG + L+A S GL Sbjct: 412 G--LAQAIVQAGSGAGVLRLQASSPGL 436 >ref|ZP_00065376.1| COG3250: Beta-galactosidase/beta-glucuronidase [Microbulbifer degradans 2-40] Length = 961 Score = 257 bits (657), Expect = 2e-67, Method: Compositional matrix adjust. Identities = 143/341 (41%), Positives = 193/341 (56%), Gaps = 7/341 (2%) Query: 1 MNHGWYEN-GYNEVVDITGYNYGHRDHLDIRDHEQHPGRLMIGSECASYTATRGIYENDP 59 MN G++ + + VD+ G NY D+ R H P SE S RG + D Sbjct: 475 MNGGFFSDLNVSHAVDVLGANYQVPDY--DRFHAARPEMPFTSSEDTSAFMMRGEFTTDY 532 Query: 60 VRGYCSEYGTNIPSWGCTPQQAWSDVVNNRFLTGVFMWTGFDYRGEPTPYLWPCVNSHFG 119 + + Y + WG + + AW V ++ G F+WTGFDYRGEPTP WP V+S FG Sbjct: 533 DKNLIASYDEDFAFWGNSHRDAWQAVATRDYVAGAFVWTGFDYRGEPTPLAWPSVSSFFG 592 Query: 120 LMDTCGFPKDSYYYMQAVW-KDEPIVHLLPHWNWPGSEGKPVEVRVFANTETAELILNGS 178 +MD GF K +YY +A W KDEP +L PHWNW G EG+ + V V AN + +L+LNG Sbjct: 593 IMDLNGFAKTAYYIHRAQWVKDEPQAYLAPHWNWAGKEGQIIPVLVMANVDKVQLLLNGK 652 Query: 179 SLGEQQVDRTGYLTWEVHYEPGELKVIGRNGGKEAAVKKAVTAGQPRQIRLYPDRAAGQA 238 SLGEQ VD T++V Y+PG+L+VIG + GKE T G+ ++L PDR A Sbjct: 653 SLGEQVVDPFQMNTFDVAYQPGKLEVIGYSSGKEVVRNSVETTGKAVAVQLVPDRKALVG 712 Query: 239 DGMDTIPVRVAVLDELGHVVPTADHEIRFEVEGAGILLGVGNGNPSSHEPDKSPVRRAFN 298 DG D +P+ V +D G VVPT + I FE+ GAG +G GNGNP+SHE +K R FN Sbjct: 713 DGFDAMPITVQAVDAKGRVVPTDNSLIHFEISGAGQSIGHGNGNPNSHEDEKGATRHLFN 772 Query: 299 GWCLXXXXIQAAPE-AGPVTLRAVSTGLAPAEVLLHFSAVT 338 G L +Q+ E +G +T++A S GL A+V + V Sbjct: 773 G--LAQLIVQSHYESSGNITVKASSPGLKTAKVSIPVKKVA 811 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.448 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 576,008,427 Number of extensions: 26039139 Number of successful extensions: 48531 Number of sequences better than 1.0e-05: 36 Number of HSP's gapped: 48422 Number of HSP's successfully gapped: 42 Length of query: 338 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 212 Effective length of database: 415,135,104 Effective search space: 88008642048 Effective search space used: 88008642048 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)