BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0014.p (457 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_636630.1| beta-galactosidase [Xanthomonas campestris pv. ... 297 3e-79 ref|NP_641643.1| beta-galactosidase [Xanthomonas axonopodis pv. ... 296 6e-79 ref|ZP_00094424.1| COG3250: Beta-galactosidase/beta-glucuronidas... 268 3e-70 ref|ZP_00065376.1| COG3250: Beta-galactosidase/beta-glucuronidas... 251 2e-65 ref|NP_625069.1| putative secreted beta-galactosidase [Streptomy... 238 2e-61 >ref|NP_636630.1| beta-galactosidase [Xanthomonas campestris pv. campestris str. ATCC 33913] gb|AAM40554.1| beta-galactosidase [Xanthomonas campestris pv. campestris str. ATCC 33913] Length = 926 Score = 297 bits (761), Expect = 3e-79, Method: Compositional matrix adjust. Identities = 171/463 (36%), Positives = 244/463 (52%), Gaps = 24/463 (5%) Query: 2 STAAGRSRSRFDENWSFYKGDIRIP-HAVKAGMTGGITDCGSLQEGEWLDIAFNDKGMGH 60 ++ A R R FD W F+ G P + G +AF+D Sbjct: 125 ASLAPRERLLFDFGWRFHPGHASDPSRDFEFGTFQRTFAKAGKDTATAAQLAFDDS---- 180 Query: 61 QQLEWKPVSLPHDWCVEQQYVQD---ASLGSRD--GSHGYLPGGTGF-------YRKTFE 108 W+ V LPHDW V + + AS+ D +HGY GT F YR+ + Sbjct: 181 ---NWQQVDLPHDWAVTLPFRPEPISASMTEEDPAAAHGYKALGTSFPENSVGWYRRMLQ 237 Query: 109 LPPEAADRKWTVTFDGASGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVILV 168 +P ++ + FDG V+ NGH++G + GY GF DL++VL YG N+I+V Sbjct: 238 IPASDLGKRICLVFDGVFRDCVVFCNGHIVGRNASGYCGFEVDLSEVLDYGKP--NLIVV 295 Query: 169 KVEALENEGWWYEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTRICNEY 228 +V+A EGW+YEG GIYRH+WL++TD LHV ++G ++ + + + A +ST + N+ Sbjct: 296 RVDATLGEGWFYEGAGIYRHLWLQKTDPLHVPQHGVFVRS-SVQGDTATAQLSTEVRNDG 354 Query: 229 TESRAVSLHTEVYNAAGEQVCAKAEQAFADWYGETEL-EQMFRLEHPALWSPETPHLYKA 287 T SR + + G V A A G+ ++ EQ L ALWS +TP LY Sbjct: 355 TASRQCVVQARITAPYGRIVAQAASAAVTVAPGQVQVVEQTVPLGQAALWSIDTPQLYTL 414 Query: 288 VSILKVEGTEVDRYETPFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVALPDSL 347 + + G VD TPFG+RSI FD + GF LNG PL + GT NHQD AGVG A+PD+L Sbjct: 415 TTSVHSAGVAVDALVTPFGVRSIAFDAQRGFLLNGAPLKLHGTNNHQDHAGVGTAIPDAL 474 Query: 348 IEYKLRLLKEMGSNAYRSAHHPPTPEXXXXXXXXXXXVMDENRKLDSSRSGLDQLKRMLF 407 ++LR LK MG NAYRS+H+P TPE V++E R++ + + +L+ M+ Sbjct: 475 HAWRLRQLKSMGCNAYRSSHNPATPELLALCDRLGMFVIEETRRMSTDPEAMAELETMVR 534 Query: 408 RDRNHPSVIIWNLENEEVLEGTVTGRRLLKTLADTVRXXXSIR 450 R RNHPSVI+W+L NEE + T G R++ + VR R Sbjct: 535 RGRNHPSVILWSLGNEEPQQVTERGARIVTRMQQRVRQLDPTR 577 >ref|NP_641643.1| beta-galactosidase [Xanthomonas axonopodis pv. citri str. 306] gb|AAM36179.1| beta-galactosidase [Xanthomonas axonopodis pv. citri str. 306] Length = 854 Score = 296 bits (758), Expect = 6e-79, Method: Compositional matrix adjust. Identities = 170/466 (36%), Positives = 243/466 (52%), Gaps = 36/466 (7%) Query: 5 AGRSRSRFDENWSFYKG-------DIRIPHAVKAGMTGGITDCGSLQEGEWLDIAFNDKG 57 A R R FD W F+ G D + G + Q +AF+D Sbjct: 56 APRERLLFDFGWRFHLGHGSDAARDFEFGTFQRTFAKAGKDTATAAQ------LAFDDSA 109 Query: 58 MGHQQLEWKPVSLPHDWCVEQQYVQD---ASLGSRD--GSHGYLPGGTGF-------YRK 105 W+ V LPHDW V + Q+ AS+ D +HGY P GT F YR+ Sbjct: 110 -------WQQVDLPHDWAVGLPFRQEPISASITEEDPAAAHGYKPLGTSFPENSVGWYRR 162 Query: 106 TFELPPEAADRKWTVTFDGASGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNV 165 T ++P ++ ++ FDG V+ NGH++G + GY GF DL+DVL YG N+ Sbjct: 163 TLQIPASDLGKRISLVFDGVFRECIVFCNGHIVGRNASGYCGFEVDLSDVLDYGKP--NI 220 Query: 166 ILVKVEALENEGWWYEGCGIYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTRIC 225 I ++V+A EGW+YEG GIYRHVWL++TD +H+ + G ++ + + A +ST + Sbjct: 221 IAIRVDATLGEGWFYEGAGIYRHVWLQKTDPVHIPQDGVFVRS-TVQDGNATAQLSTEVR 279 Query: 226 NEYTESRAVSLHTEVYNAAGEQVCAKAEQAFADWYGETEL-EQMFRLEHPALWSPETPHL 284 NE + R + + G V + G+ ++ EQ L LWS + P L Sbjct: 280 NEGSAPRRCVVQARITAPDGRTVAQASSAVATVAPGQVQVVEQTLPLGQAVLWSIDAPQL 339 Query: 285 YKAVSILKVEGTEVDRYETPFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVALP 344 Y + + +GT VD TPFG+RSI FD + GF LNG PL + GT NHQD AGVG A+P Sbjct: 340 YHLTTSVHSDGTAVDALVTPFGVRSIAFDAQRGFLLNGAPLKLHGTNNHQDHAGVGTAIP 399 Query: 345 DSLIEYKLRLLKEMGSNAYRSAHHPPTPEXXXXXXXXXXXVMDENRKLDSSRSGLDQLKR 404 D+L ++LR LK MG NAYRS+H+P TPE V++E R++ + + +L+ Sbjct: 400 DALHAWRLRQLKSMGCNAYRSSHNPATPELLALCDRLGMLVIEETRRMSTDPEAMGELET 459 Query: 405 MLFRDRNHPSVIIWNLENEEVLEGTVTGRRLLKTLADTVRXXXSIR 450 M+ R RNHPSVI+W+L NEE + T G R++ + VR R Sbjct: 460 MVRRGRNHPSVILWSLGNEEPQQVTARGARIVTRMQQRVRQLDPTR 505 >ref|ZP_00094424.1| COG3250: Beta-galactosidase/beta-glucuronidase [Novosphingobium aromaticivorans] Length = 691 Score = 268 bits (684), Expect = 3e-70, Method: Compositional matrix adjust. Identities = 151/444 (34%), Positives = 234/444 (52%), Gaps = 19/444 (4%) Query: 8 SRSRFDENWSFYKGDIRIPHAVKAGMTGGITDCGSLQEGEWLDIAFNDKGMGHQQLEWKP 67 SR DE W F++GD+ +P T G+ A M +W Sbjct: 62 SRLLLDEGWRFHEGDVVVPPPASHHETYLSVKAGN---------ARGAAAMAWDDSDWPV 112 Query: 68 VSLPHDWCVEQQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWTVTFDGASG 127 V +PHDW Q +V++A++ S GY P G G+YR+TF L ++ + F G + Sbjct: 113 VRVPHDWASAQPFVREANV-----SQGYRPRGIGWYRRTFRLAEADRGKRLELQFGGVAT 167 Query: 128 LSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVILVKVEALENEGWWYEGCGIYR 187 +W+NG L+ + Y + D+T R+G E NVI V+V+A EGWWYEG GIYR Sbjct: 168 ACQIWINGSLVARNSSAYNAINIDITPFARFGEED-NVIAVRVDADALEGWWYEGAGIYR 226 Query: 188 HVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTRICNEYTESRAVSLHTEVYNAAGEQ 247 HVWL + + +A G + P + V ++ + + +R V++ + + AG + Sbjct: 227 HVWLARRSGVSIATDGVH-CDPRLVDGRWWVPVTVTLESVEKLARTVTVTAVLRDGAGRE 285 Query: 248 VCAKAEQAFADWYGETELEQMFRLEHPALWSPETPHLYKA-VSILKVEGTEVDRYETPFG 306 V A + G E + +PALWS ETP LY+ ++ ++G ++ R P G Sbjct: 286 VARGEAHAQVEPLGRAEARVTLEVGNPALWSVETPTLYEVEITAGHLDGMDLRR--VPVG 343 Query: 307 IRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVALPDSLIEYKLRLLKEMGSNAYRSA 366 R+IRFD E+GFFLN +P+ +KG C HQD AGVGVA+PD+L+ ++L LK+MG NA R + Sbjct: 344 FRTIRFDAEKGFFLNDKPVKLKGVCLHQDHAGVGVAIPDALLAWRLARLKDMGCNAIRCS 403 Query: 367 HHPPTPEXXXXXXXXXXXVMDENRKLDSSRSGLDQLKRMLFRDRNHPSVIIWNLENEEVL 426 H+ + VMDENR + + + QL+ ++ RNHPS+++W++ NEE + Sbjct: 404 HNAQSEAFYALCDRIGFLVMDENRVFNPAPENMAQLEWLVRAHRNHPSIVLWSVFNEEPM 463 Query: 427 EGTVTGRRLLKTLADTVRXXXSIR 450 +GT G +++ + VR R Sbjct: 464 QGTTQGVEMVRRMRAAVRALDDSR 487 >ref|ZP_00065376.1| COG3250: Beta-galactosidase/beta-glucuronidase [Microbulbifer degradans 2-40] Length = 961 Score = 251 bits (641), Expect = 2e-65, Method: Compositional matrix adjust. Identities = 146/450 (32%), Positives = 233/450 (51%), Gaps = 21/450 (4%) Query: 6 GRSRSRFDENWSFYKGDIRIPHAVKAGMTGGITDCGSLQEGEWLDIAFNDKGMGHQQLEW 65 GR R + W F+ GD+ P A G + + + F+D EW Sbjct: 36 GRERLSLNRGWYFHLGDV--PMAPIKGHSESYMNAKAGNAPGPAGREFDDS-------EW 86 Query: 66 KPVSLPHDWCVEQQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWTVTFDGA 125 + + LPHDW VE + + ++ S GY P G +YR+ + R + + FD Sbjct: 87 RRLDLPHDWAVEGPFDPNENI-----SQGYRPRGISWYRRYLRVEESDRGRYFELQFDAI 141 Query: 126 SGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVILVKVEALENEGWWYEGCGI 185 + +TVWVNG+++ + GY D+T +RYG + N I VKV+A + EGWWYEG G+ Sbjct: 142 ATHATVWVNGNVVKRNYSGYNASYIDITPYIRYG-DAMNTIAVKVDATQMEGWWYEGAGM 200 Query: 186 YRHVWLEQTDRLHVAEYGTYITTPEAEKE-----QALVHISTRICNEYTESRAVSLHTEV 240 YRH WL + + +H+ G + TP E E + + + + N + V++ V Sbjct: 201 YRHTWLVKANPVHIVTDGLH-ATPRLESETLEGGKWSIPVEVTLNNSGENLQTVTVEVTV 259 Query: 241 YNAAGEQVCAKAEQAFADWYGETELEQMFRLEHPALWSPETPHLYKAVSILKVEGTEVDR 300 G+QV ++ GE + ++ PALWS E +LY+ +++K +D Sbjct: 260 TAPNGKQVAKQSGNVTVPVLGEAVAKLPVTIQSPALWSHEQTNLYRVNAVVKHGKRVIDE 319 Query: 301 YETPFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVALPDSLIEYKLRLLKEMGS 360 G R++RFD ++GFFLN + + ++G C HQD AGVGVA+P S+ +Y+LR LKE+G Sbjct: 320 IALSTGFRTVRFDSQQGFFLNDKHVKLQGVCIHQDHAGVGVAVPTSIWQYRLRRLKELGV 379 Query: 361 NAYRSAHHPPTPEXXXXXXXXXXXVMDENRKLDSSRSGLDQLKRMLFRDRNHPSVIIWNL 420 NA R +H+ P E VMDENR + S + QL+ M+ RDR+HP +I+W++ Sbjct: 380 NAIRFSHNAPAVEVLDLVDSMGFLVMDENRNFNPSPDYMQQLEWMVRRDRHHPGIILWSV 439 Query: 421 ENEEVLEGTVTGRRLLKTLADTVRXXXSIR 450 NEE ++ + G ++++ + V+ R Sbjct: 440 FNEEPVQASEVGYQMVRRMVAAVKALDDTR 469 >ref|NP_625069.1| putative secreted beta-galactosidase [Streptomyces coelicolor A3(2)] emb|CAB61538.1| putative secreted beta-galactosidase [Streptomyces coelicolor A3(2)] Length = 1028 Score = 238 bits (607), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 139/401 (34%), Positives = 199/401 (49%), Gaps = 23/401 (5%) Query: 65 WKPVSLPHDWCVEQQYVQDASLGSRDGSHGYLPGGTGFYRKTFELPPEAADRKWTVTFDG 124 W+ V++PHDW +EQ + G+ G+ G+LPGG G+YR F LPP A ++ +V FDG Sbjct: 75 WREVAVPHDWSIEQTPTTEH--GTTSGT-GFLPGGLGWYRLAFTLPPALAGKRISVEFDG 131 Query: 125 ASGLSTVWVNGHLIGEHRGGYIGFSYDLTDVLRYGAEGRNVILVKVEALENEGWWYEGCG 184 S V NG +G H GY GF+ DLTD++ NV+ V+V WY G G Sbjct: 132 VYMDSRVHCNGREVGHHPYGYTGFALDLTDLVHTDGRTENVLAVRVRNQLPSSRWYSGSG 191 Query: 185 IYRHVWLEQTDRLHVAEYGTYITTPEAEKEQALVHISTRICNEYTESRAVSLHTEVYNAA 244 IYR L T+ +HV +GTY+TTP E A+V + T + N +R V + + V +A Sbjct: 192 IYREARLVVTEPVHVRRWGTYVTTPRVSGESAVVRVRTSVVNATGTAREVEIRSAVKDAD 251 Query: 245 GEQVCAKAEQA-FADWYGETELEQMFRLEHPALWSPETPHLYKAVSILKVEGTEVDRYET 303 G V A D ET + R P LW +PH Y + L+V G VD Y T Sbjct: 252 GRTVARTASTVDVTDAASETHELTVAR---PRLWDFASPHRYTLHTELRVGGRTVDTYRT 308 Query: 304 PFGIRSIRFDPEEGFFLNGEPLLIKGTCNHQDFAGVGVALPDSLIEYKLRLLKEMGSNAY 363 PFGIR+ DPEEGF LNG IKG H D +G A+ + ++ L+K MG NA+ Sbjct: 309 PFGIRTFHVDPEEGFHLNGRYAKIKGVDLHHDLGALGAAVSADAVRRQMTLMKSMGVNAF 368 Query: 364 RSAHHPPTPEXXXXXXXXXXXVMDENRKLDSSRSGLDQ--------------LKRMLFRD 409 R++H+PP+PE ++ E D R+G ++ M+ Sbjct: 369 RTSHNPPSPEMIEVCEEMGIIMLVE--AFDCWRTGKNRYDYGRFFDEWCERDATEMVLAA 426 Query: 410 RNHPSVIIWNLENEEVLEGTVTGRRLLKTLADTVRXXXSIR 450 RN P+V++W++ NE G + + + +R R Sbjct: 427 RNSPAVVMWSIGNEVPDSTATAGLAMADRIIEAIRAADDTR 467 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.136 0.425 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 725,638,988 Number of extensions: 31735920 Number of successful extensions: 58160 Number of sequences better than 1.0e-05: 326 Number of HSP's gapped: 57528 Number of HSP's successfully gapped: 327 Length of query: 457 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 328 Effective length of database: 406,989,807 Effective search space: 133492656696 Effective search space used: 133492656696 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 128 (53.9 bits)