BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0013.p (342 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00080427.1| COG2843: Putative enzyme of poly-gamma-glutam... 228 1e-58 ref|NP_831556.1| Capsule biosynthesis protein capA [Bacillus cer... 208 1e-52 ref|NP_978256.1| conserved hypothetical protein [Bacillus cereus... 207 3e-52 ref|NP_951098.1| capsule biosynthesis protein, putative [Geobact... 198 2e-49 ref|NP_844272.1| conserved hypothetical protein [Bacillus anthra... 198 2e-49 >ref|ZP_00080427.1| COG2843: Putative enzyme of poly-gamma-glutamate biosynthesis (capsule formation) [Geobacter metallireducens] Length = 349 Score = 228 bits (582), Expect = 1e-58, Method: Compositional matrix adjust. Identities = 128/339 (37%), Positives = 188/339 (55%), Gaps = 22/339 (6%) Query: 3 RFAVITLVLFLLIPGQAAFSQGASPQEKINLAFAGDILLDGFVGDQIAKYGVNFPFAKVA 62 R + +V L +P A + I + GDI+L G +++ G ++PFAK A Sbjct: 5 RLMLAAIVTLLAVPALGA--------DGIVITCVGDIMLAGSATPTLSRSGYDYPFAKTA 56 Query: 63 PALKKADIAFANLETPVSVRG-EAAEKTFAFRSKPAALAGLNAAGIDGVTLANNHILDFG 121 L++ DIA NLE P++ RG E +KT+ FR+ P A A L AG +TLANNH++D+G Sbjct: 57 QELRRGDIAMGNLEAPLTERGTEYRDKTYRFRTNPIAAAALKRAGFSVLTLANNHMMDYG 116 Query: 122 QDAMLDTLVHLDKYKIGHTGAGKNSNEAFRPYVQTVKNQKIAILGVSRVLSSPS-WYAGT 180 + + DTL L ++ I HTGAG + EA R V +V+ ++IA L S L+ PS +YAG Sbjct: 117 NEGLQDTLATLSRHGIAHTGAGASLAEARREAVVSVRGKRIAFLAYS--LTFPSEFYAGP 174 Query: 181 NSPGAASAYTPEPMLGAIKKSAKENDYTIVYIHWNNEFKDYPEPYARNLAKQMIDSGADI 240 N PG A Y P + I+++ E DY +V HW E ++P+ Y A+ ID+GAD Sbjct: 175 NRPGTAPGYAPH-VREDIRRAKAEADYVVVSFHWGAERAEFPKQYQTETARLAIDAGADA 233 Query: 241 IIGAHSHCLMGIEYYKHKPIYYSLGNFVFNRSTRGGDKTIHSMLVNFEISNNRVKSRITP 300 IIG H H L GIE+Y+ KPI YSLGNF F + D+ S++ +S+ + P Sbjct: 234 IIGHHPHVLQGIEFYRGKPILYSLGNFAFGSRSTAADR---SVMARLTLSDEETSVELVP 290 Query: 301 VKI----TGGQPNFMGEAYNKDTIKRMNQLS--FNAVIA 333 + + T QP + + I+R+N+LS F VI+ Sbjct: 291 LNVLHRETRYQPGILAGRKGAEVIERLNRLSQPFGTVIS 329 >ref|NP_831556.1| Capsule biosynthesis protein capA [Bacillus cereus ATCC 14579] gb|AAP08757.1| Capsule biosynthesis protein capA [Bacillus cereus ATCC 14579] Length = 367 Score = 208 bits (530), Expect = 1e-52, Method: Compositional matrix adjust. Identities = 111/324 (34%), Positives = 171/324 (52%), Gaps = 3/324 (0%) Query: 19 AAFSQGASPQEKINLAFAGDILLDGFVGDQIAKYGVNFPFAKVAPALKKADIAFANLETP 78 A+ S+ ++I L F+GD + D + I K G ++PF V +KKADI+F NLE+ Sbjct: 44 ASKSEKKIEDQEITLTFSGDTMFDWQLRPIIEKNGADYPFQHVKEEIKKADISFVNLESA 103 Query: 79 VSVRGEAAE-KTFAFRSKPAALAGLNAAGIDGVTLANNHILDFGQDAMLDTLVHLDKYKI 137 + + + A + F +S P+ L + G D V + NNH LD+GQD +LDT+ H++K K Sbjct: 104 FTTKEKKAPGQLFWIKSDPSTLQAIKNTGYDIVNIGNNHTLDYGQDGLLDTISHVEKLKF 163 Query: 138 GHTGAGKNSNEAFRPYVQTVKNQKIAILGVSRVLSSPSWYAGTNSPGAASAYTPEPMLGA 197 + GAGKN+ +A+ TVK +K L R + +W AG N PG A+ Y + Sbjct: 164 PYIGAGKNAKDAYTAREMTVKGKKFKFLSFVRFMPDSNWVAGENKPGVANGYDLNLVTKT 223 Query: 198 IKKSAKENDYTIVYIHWNNEFKDYPEPYARNLAKQMIDSGADIIIGAHSHCLMGIEYYKH 257 IK+ K+ DY IVY+HW E + P Y + +M+++GA+ I+G+H H L G EYY Sbjct: 224 IKEQKKDADYLIVYMHWGVEKSNRPVEYQKQYVPKMVEAGANAIVGSHPHWLQGFEYYNK 283 Query: 258 KPIYYSLGNFVFNRSTRGGDKTIHSMLVNFEISNNRVKSRITPVKITGGQPNFMGEAYNK 317 PI YSLGNF+F G K+ + ++ V+ P I Q + + E K Sbjct: 284 VPIAYSLGNFLFPSYVNG--KSAETGVLTLTFKGKDVQMSFNPYIIRNNQVSPVNEGEKK 341 Query: 318 DTIKRMNQLSFNAVIAADGAVSEK 341 ++ + +S + I G + K Sbjct: 342 KALQYLQTISTDVEIDDTGKIKNK 365 >ref|NP_978256.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] emb|CAB40617.1| capA [Bacillus cereus] gb|AAS40864.1| conserved hypothetical protein [Bacillus cereus ATCC 10987] Length = 367 Score = 207 bits (527), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 115/331 (34%), Positives = 171/331 (51%), Gaps = 9/331 (2%) Query: 18 QAAFSQGASPQEK------INLAFAGDILLDGFVGDQIAKYGVNFPFAKVAPALKKADIA 71 Q S+ AS EK I L F+GD + D + I K G ++PF V + KADI+ Sbjct: 37 QNKSSKTASTSEKKIENPEITLTFSGDTMFDWQLRPVIEKNGADYPFQHVKEEITKADIS 96 Query: 72 FANLETPVSVRGEAAE-KTFAFRSKPAALAGLNAAGIDGVTLANNHILDFGQDAMLDTLV 130 F NLE+ + R + A + F +S P+ L + G D V + NNH LD+GQD +LDT+ Sbjct: 97 FVNLESAFTTREKKAPGQLFWIKSDPSTLQAIKNTGYDIVNIGNNHTLDYGQDGLLDTIS 156 Query: 131 HLDKYKIGHTGAGKNSNEAFRPYVQTVKNQKIAILGVSRVLSSPSWYAGTNSPGAASAYT 190 H++K K + GAGKN+ +A+ TVK +K L R + +W AG N PG A+ Y Sbjct: 157 HVEKLKFPYIGAGKNAKDAYTAREMTVKGKKFKFLSFVRFMPDTNWVAGDNKPGVANGYD 216 Query: 191 PEPMLGAIKKSAKENDYTIVYIHWNNEFKDYPEPYARNLAKQMIDSGADIIIGAHSHCLM 250 + IK+ K+ DY IVY+HW E + P Y + +M+++GAD I+G+H H L Sbjct: 217 LNLVTKTIKEQKKDADYLIVYMHWGVEKSNRPVEYQKQYVPKMVEAGADAIVGSHPHWLQ 276 Query: 251 GIEYYKHKPIYYSLGNFVFNRSTRGGDKTIHSMLVNFEISNNRVKSRITPVKITGGQPNF 310 G EYY PI YSLGNF+F G K+ + ++ V+ P I Q + Sbjct: 277 GFEYYNKVPIAYSLGNFLFPSYVNG--KSAETGVLTLTFKGKDVQMSFNPYIIRNNQVSP 334 Query: 311 MGEAYNKDTIKRMNQLSFNAVIAADGAVSEK 341 + E + ++ + +S + I G + K Sbjct: 335 VNEEEKRKALQYLQTISTDVDIDDTGNIKNK 365 >ref|NP_951098.1| capsule biosynthesis protein, putative [Geobacter sulfurreducens PCA] gb|AAR33371.1| capsule biosynthesis protein, putative [Geobacter sulfurreducens PCA] Length = 351 Score = 198 bits (503), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 112/312 (35%), Positives = 166/312 (53%), Gaps = 14/312 (4%) Query: 29 EKINLAFAGDILLDGFVGDQIAKYGVNFPFAKVAPALKKADIAFANLETPVSVRG-EAAE 87 E+I+L+F GD++L G D + +YG ++PF+ A L+++D+ NLE P++ G E Sbjct: 24 ERISLSFVGDVMLAGSATDTLQRYGYSYPFSATAAELRRSDLVVGNLEAPLTDGGREFRA 83 Query: 88 KTFAFRSKPAALAGLNAAGIDGVTLANNHILDFGQDAMLDTLVHLDKYKIGHTGAGKNSN 147 K F F++ P A A L AG +TLANNH++DFG D + DT+ HL++ I GAG + Sbjct: 84 KRFRFKASPVAAAALKRAGFSVMTLANNHMMDFGADGLSDTIHHLNRNGIAFAGAGPSIA 143 Query: 148 EAFRPYVQTVKNQKIAILGVSRVLSSP-SWYAGTNSPGAASAYTPEPMLGAIKKSAKEND 206 +A R TV+ Q +A L S L+ P ++A PG A Y L I++ D Sbjct: 144 DARREASVTVRGQTVAFLAYS--LTQPIEFFATEGRPGTAPGYAGH-YLADIRRVRSSAD 200 Query: 207 YTIVYIHWNNEFKDYPEPYARNLAKQMIDSGADIIIGAHSHCLMGIEYYKHKPIYYSLGN 266 + +V HW E P PY A + ID+GADI+IG H H L GIE Y+ PI+YSLGN Sbjct: 201 HVVVSFHWGQERAALPSPYQIETAHRAIDAGADIVIGHHPHVLQGIEIYRGSPIFYSLGN 260 Query: 267 FVFNRSTRGGDKTIHSMLVNFEISNNRVKSRITPVKI----TGGQPNFMGEAYNKDTIKR 322 F F + D++I + + + P+ + QP + D + R Sbjct: 261 FAFGSRSPSADRSI---IARVTLGEGPPIVEVIPLNVLFREVRFQPAILTGRKAADVVDR 317 Query: 323 MNQLS--FNAVI 332 +N+LS F+ VI Sbjct: 318 LNRLSAPFSTVI 329 >ref|NP_844272.1| conserved hypothetical protein [Bacillus anthracis str. Ames] gb|AAP25758.1| conserved hypothetical protein [Bacillus anthracis str. Ames] Length = 367 Score = 198 bits (503), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 105/313 (33%), Positives = 164/313 (52%), Gaps = 3/313 (0%) Query: 30 KINLAFAGDILLDGFVGDQIAKYGVNFPFAKVAPALKKADIAFANLETPVSVRGEAAE-K 88 +I L F+GD + D + I K G ++PF V + KADI+F NLE+ + + + A + Sbjct: 55 EITLTFSGDTMFDWQLRPVIEKNGADYPFQHVKEEITKADISFVNLESAFTTKEKKAPGQ 114 Query: 89 TFAFRSKPAALAGLNAAGIDGVTLANNHILDFGQDAMLDTLVHLDKYKIGHTGAGKNSNE 148 F +S P+ L + G D V + NNH LD+GQ+ +LDT+ H++K K + GAGKN+ + Sbjct: 115 LFWIKSDPSTLQAIKNTGYDIVNIGNNHTLDYGQNGLLDTISHVEKLKFPYIGAGKNAKD 174 Query: 149 AFRPYVQTVKNQKIAILGVSRVLSSPSWYAGTNSPGAASAYTPEPMLGAIKKSAKENDYT 208 A+ TVK +K L R + +W A N PG A+ Y + IK+ K+ DY Sbjct: 175 AYTAREMTVKGKKFKFLSFVRFMPDFTWVADDNKPGVANGYDLNLVTKTIKEQKKDADYL 234 Query: 209 IVYIHWNNEFKDYPEPYARNLAKQMIDSGADIIIGAHSHCLMGIEYYKHKPIYYSLGNFV 268 IVY+HW E + P Y + +M+ +GAD I+G+H H L G EYY + P+ YSLGNF+ Sbjct: 235 IVYMHWGVEKSNRPVDYQKQYVNKMVTAGADAIVGSHPHWLQGFEYYNNVPVAYSLGNFL 294 Query: 269 FNRSTRGGDKTIHSMLVNFEISNNRVKSRITPVKITGGQPNFMGEAYNKDTIKRMNQLSF 328 F G K+ + ++ V+ P I Q + + E + ++ + +S Sbjct: 295 FPSYVNG--KSAETGVLTLTFKGKDVQMSFNPYIIRNNQVSPVNEEEKRKALQYLQTIST 352 Query: 329 NAVIAADGAVSEK 341 + I G + K Sbjct: 353 DVEIDDTGKIINK 365 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 515,788,492 Number of extensions: 22316988 Number of successful extensions: 60881 Number of sequences better than 1.0e-05: 91 Number of HSP's gapped: 60749 Number of HSP's successfully gapped: 94 Length of query: 342 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 216 Effective length of database: 415,135,104 Effective search space: 89669182464 Effective search space used: 89669182464 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)