BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0002.p (345 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB96938.1| yndF [Bacillus cereus] 184 3e-45 ref|NP_832853.1| Spore germination protein BC [Bacillus cereus A... 184 3e-45 ref|NP_389660.1| similar to spore germination protein [Bacillus ... 172 2e-41 ref|NP_391463.1| gerBC [Bacillus subtilis] >gi|729630|sp|P39571|... 136 6e-31 ref|ZP_00060529.1| hypothetical protein [Clostridium thermocellu... 111 3e-23 >emb|CAB96938.1| yndF [Bacillus cereus] Length = 390 Score = 184 bits (467), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 103/361 (28%), Positives = 200/361 (55%), Gaps = 26/361 (7%) Query: 1 LSIIQGVAIDTNQD----------------MLELTYQHLIAQ-----KSQKNTYTNVTTL 39 LS+ G+A+D +D ++ TYQ + +Q Q+ Y NV+ Sbjct: 28 LSLTAGIALDKGKDSTIEKEDEEGGYPKRNIITATYQIVSSQAQSKGNGQQKRYINVSET 87 Query: 40 NKDSIQAASRDQAKLVSRSPLYN-FIRIVLISDQALEHTRIDQLLDTFTRSYKPSRKSLV 98 DSI R+ + L S +++ ++ ++ISD + ++QLL+ + R + ++ Sbjct: 88 G-DSIHQIVREFS-LKRESTMFSPHLKNIVISDSLVRTYSLEQLLEQYLRDNEVRLSCML 145 Query: 99 MIVKGNAKEALNMLGKHKEMPSIDLEELADNSAFNSKIPRKIRFGEISIHISQGTDFIIQ 158 +I KG AKE L K E+P+ L +ADN ++I + ++ + G+ F++Q Sbjct: 146 LISKGLAKEVLES-NKKGEIPAFRLSGIADNRNRTTRIVPPVSLAKLEGKMRSGSSFLLQ 204 Query: 159 R-LETNEDNHLSGAALVSGKTKRFAGWVNEAEVTGINWIQGRSKGSIIKVEDPKTQQHMV 217 + + +GAA++ GKTK+ G++NE E+ G+ WI G+ G ++K D ++++ ++ Sbjct: 205 NVISIKGETKFAGAAVIKGKTKKLMGFLNEQELEGVVWINGKRNGGVLKTFDKESKKLII 264 Query: 218 FEVEDVHSKLIPRVNGKDLSFTVRIKTDIKLNETSVISAELLKEPFIRSAKETVEDEIRR 277 +E++ + SK+IP VNG ++SF V I+++ +L+E + S + + F++ ++ +E E++ Sbjct: 265 YEIKSIKSKIIPHVNGNNISFDVNIESEGRLSENWIQSGKDFENEFLKREEKAIEKEVKH 324 Query: 278 MALQSLNKLQKELRADVAGFGTKVKVNYPAYWDQVKDKWQDTFSEIPVNVQVKVQIERTG 337 + K+QKE + D+AGFG ++++ +P W++VK W TFS++P+ + V I+ G Sbjct: 325 LVHHVTKKIQKEYQVDIAGFGNQLRIKHPRTWEKVKKNWDQTFSKVPIKYHINVTIQDYG 384 Query: 338 A 338 A Sbjct: 385 A 385 >ref|NP_832853.1| Spore germination protein BC [Bacillus cereus ATCC 14579] emb|CAB69789.1| putative spore germination protein [Bacillus cereus] gb|AAP10054.1| Spore germination protein BC [Bacillus cereus ATCC 14579] Length = 397 Score = 184 bits (467), Expect = 3e-45, Method: Compositional matrix adjust. Identities = 103/361 (28%), Positives = 200/361 (55%), Gaps = 26/361 (7%) Query: 1 LSIIQGVAIDTNQD----------------MLELTYQHLIAQ-----KSQKNTYTNVTTL 39 LS+ G+A+D +D ++ TYQ + +Q Q+ Y NV+ Sbjct: 35 LSLTAGIALDKGKDSTIEKEDEEGGYPKRNIITATYQIVSSQAQSKGNGQQKRYINVSET 94 Query: 40 NKDSIQAASRDQAKLVSRSPLYN-FIRIVLISDQALEHTRIDQLLDTFTRSYKPSRKSLV 98 DSI R+ + L S +++ ++ ++ISD + ++QLL+ + R + ++ Sbjct: 95 G-DSIHQIVREFS-LKRESTMFSPHLKNIVISDSLVRTYSLEQLLEQYLRDNEVRLSCML 152 Query: 99 MIVKGNAKEALNMLGKHKEMPSIDLEELADNSAFNSKIPRKIRFGEISIHISQGTDFIIQ 158 +I KG AKE L K E+P+ L +ADN ++I + ++ + G+ F++Q Sbjct: 153 LISKGLAKEVLES-NKKGEIPAFRLSGIADNRNRTTRIVPPVSLAKLEGKMRSGSSFLLQ 211 Query: 159 R-LETNEDNHLSGAALVSGKTKRFAGWVNEAEVTGINWIQGRSKGSIIKVEDPKTQQHMV 217 + + +GAA++ GKTK+ G++NE E+ G+ WI G+ G ++K D ++++ ++ Sbjct: 212 NVISIKGETKFAGAAVIKGKTKKLMGFLNEQELEGVVWINGKRNGGVLKTFDKESKKLII 271 Query: 218 FEVEDVHSKLIPRVNGKDLSFTVRIKTDIKLNETSVISAELLKEPFIRSAKETVEDEIRR 277 +E++ + SK+IP VNG ++SF V I+++ +L+E + S + + F++ ++ +E E++ Sbjct: 272 YEIKSIKSKIIPHVNGNNISFDVNIESEGRLSENWIQSGKDFENEFLKREEKAIEKEVKH 331 Query: 278 MALQSLNKLQKELRADVAGFGTKVKVNYPAYWDQVKDKWQDTFSEIPVNVQVKVQIERTG 337 + K+QKE + D+AGFG ++++ +P W++VK W TFS++P+ + V I+ G Sbjct: 332 LVHHVTKKIQKEYQVDIAGFGNQLRIKHPRTWEKVKKNWDQTFSKVPIKYHINVTIQDYG 391 Query: 338 A 338 A Sbjct: 392 A 392 >ref|NP_389660.1| similar to spore germination protein [Bacillus subtilis] pir||F69889 spore germination protein homolog yndF - Bacillus subtilis emb|CAB13661.1| yndF [Bacillus subtilis subsp. subtilis str. 168] Length = 404 Score = 172 bits (435), Expect = 2e-41, Method: Compositional matrix adjust. Identities = 101/306 (33%), Positives = 170/306 (55%), Gaps = 4/306 (1%) Query: 33 YTNVTTLNKDSIQAASRDQAKLVSRSPLYNFIRIVLISDQALEHTRIDQLLDTFTRSYKP 92 Y N++ DS+Q + A R +++V++S+ L ID++LD F R + Sbjct: 97 YINISETG-DSLQQIGSEVALRRDREVFSPHLKVVVMSEDVLHTFPIDEMLDQFFRDNEI 155 Query: 93 SRKSLVMIVKGNAKEALNMLGKHKEMPSIDLEELADNSAFNSKIPRKIRFGEISIHISQG 152 LV+ KG A++AL L ++ E+P+ L L +N S+I + ++ + G Sbjct: 156 RLSCLVLSAKGEARDALQ-LKENGEIPAFRLIGLGENEHKVSRILPPMTLAKLIGKLHSG 214 Query: 153 TDFIIQRL-ETNEDNHLSGAALVSGKTKRFAGWVNEAEVTGINWIQGRSKGSIIKVEDPK 211 + F++Q + N SGAA+++GK+K+ G +NE E GI WI+G KG ++K D K Sbjct: 215 SSFLLQNVVAANGAVKYSGAAVINGKSKKMIGTLNEYETEGITWIRGEGKGGVVKSHDKK 274 Query: 212 TQQHMVFEVEDVHSKLIPRVNGKDLSFTVRIKTDIKLNETSVISAELLKEPFIRSAKETV 271 +QQ + +++ + S++ P V GKD+SF V I+++ L E + + E L FI + T+ Sbjct: 275 SQQTLAYDINKIKSRIQPIVKGKDISFHVDIESEGDLVE-NWNTKEALDTQFIDRLETTI 333 Query: 272 EDEIRRMALQSLNKLQKELRADVAGFGTKVKVNYPAYWDQVKDKWQDTFSEIPVNVQVKV 331 E+E++++ Q L K+Q + +ADVAGF ++ YP W +VK+ W DTFS+ + V V Sbjct: 334 ENEVKKIVGQVLKKIQHDYKADVAGFDESFRLTYPHLWKRVKNNWDDTFSKADITYSVNV 393 Query: 332 QIERTG 337 I G Sbjct: 394 TITHFG 399 >ref|NP_391463.1| gerBC [Bacillus subtilis] sp|P39571|GRBC_BACSU Spore germination protein B3 precursor pir||I39857 spore germination apparatus protein gerBC - Bacillus subtilis gb|AAA22468.1| gerB emb|CAB15599.1| gerBC [Bacillus subtilis subsp. subtilis str. 168] Length = 374 Score = 136 bits (343), Expect = 6e-31, Method: Compositional matrix adjust. Identities = 92/352 (26%), Positives = 175/352 (49%), Gaps = 17/352 (4%) Query: 1 LSIIQGVAID-TNQDMLELTYQHLI-----AQKSQKNTYTNVTTLNKDSIQAASRDQAKL 54 LS +G+AID TN +LTYQ+L+ +Q S K + NVT+ K ++A S K Sbjct: 29 LSFARGLAIDETNDHQYKLTYQNLLPQSEDSQASGKPEFVNVTSHGKTILEAVSDVSIK- 87 Query: 55 VSRSPLY-NFIRIVLISDQALEHTRIDQLLDTFTRSYKPSRKSLVMIVKGNAKEALNMLG 113 P+Y + ++++L+ ++ + + +DQ+L+ F R + R S +M +GNA + Sbjct: 88 --DPPVYSDHLKVILLGEKLMRNQNVDQVLNHFIRDDELRRSSYLMAARGNAADVFTKGN 145 Query: 114 KHKE--MPSIDLEELADNSAFNSKIPRKIRFGEISIHISQGTDFIIQRLETNEDN-HLSG 170 +++ MPS L +L +S +N KI +R G S++ G ++IQ ++ + G Sbjct: 146 PNQQQPMPSEKLIDLTTHSGYNGKIMIPLRIGRASVYSQNGYSYLIQAVKNEKGKAKYDG 205 Query: 171 AALVSGKTKRFAGWVNEAEVTGINWIQGRSKGSIIKVEDPKTQQHMVFEVEDVHSKLIPR 230 A ++ + + G+++ E ++W+ G +G ++ D + FE++ +K+ P Sbjct: 206 AGIIKRGSNKLVGFLSADETQTLSWVMGTIQGGVMPTTD--KGHPITFEIKKSKTKIKPV 263 Query: 231 V-NGKDLSFTVRIKTDIKLNETSVISAELLKEPFIRSAKETVEDEIRRMALQSLNKLQKE 289 + NGK + F + +KT L E + + ++ + E ++ R Q ++KLQ E Sbjct: 264 IENGKPV-FHISVKTKGILTEDQNPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHE 322 Query: 290 LRADVAGFGTKVKVNYPAYWDQVKDKWQDTFSEIPVNVQVKVQIERTGAYTK 341 + D +++ +P YW++VK W + FSE + +I G K Sbjct: 323 YKTDPVFLSDHIRIQHPDYWNKVKGHWDEIFSETDFKYDISFKIINFGTVGK 374 >ref|ZP_00060529.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 423 Score = 111 bits (277), Expect = 3e-23, Method: Compositional matrix adjust. Identities = 79/288 (27%), Positives = 149/288 (51%), Gaps = 15/288 (5%) Query: 59 PLYNFIRIVLISDQALEHTRIDQLLDTFTRSYKPSRKSLVMIVKGNAKEALNMLGKHKEM 118 P Y +++++IS++ + + +LD F R ++ ++ + I G+AK+AL+++ + ++ Sbjct: 139 PNYEHLQVIIISEK-VARKGLRNVLDLFIRDHEMRSRTKLFITDGDAKKALDVIPRIEDY 197 Query: 119 PSIDLEELADNSAFNSKIPRKIRFGEISIHISQGTDFIIQRLETNEDNHLS-GAALVSGK 177 SI L ++ ++ N +I + G+ I G DF + LE E ++ GAAL K Sbjct: 198 ASIYLTKMPRSARVNGEILHWMDLGQAVQAIYSGEDFELPALEVTEYEVMNKGAALF--K 255 Query: 178 TKRFAGWVNEAEVTGINWIQGRSKGSIIK---VEDPKTQQHMVFEVEDVHSK--LIPRVN 232 + GW + +V I + G I V D ++ V +E + SK + P + Sbjct: 256 NDKMVGWADGKDVEIIKIMHNVLLGGIFTSKFVSDEHDSENGVMSLEIIKSKTKITPVIQ 315 Query: 233 GKDLSFTVRIKTDIKLNETSVISAEL---LKEPFIRSAKETVEDEIRRMALQSLNKLQKE 289 D++F +I DIK N + ++ L + + FI A+E E+ I+ ++++ K+Q + Sbjct: 316 DDDITF--KINVDIKGNYSDSVNHPLTEKIDKDFIEKAEEAFEESIKEQCIKTIKKMQ-D 372 Query: 290 LRADVAGFGTKVKVNYPAYWDQVKDKWQDTFSEIPVNVQVKVQIERTG 337 L D FGT ++ P++W ++KD+W + F E+ V VKV I + G Sbjct: 373 LGVDTFHFGTVIRSKKPSHWSKIKDRWDEIFPEVKTEVNVKVNIRQIG 420 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.131 0.360 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 462,587,458 Number of extensions: 18227481 Number of successful extensions: 54960 Number of sequences better than 1.0e-05: 35 Number of HSP's gapped: 54912 Number of HSP's successfully gapped: 35 Length of query: 345 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 219 Effective length of database: 415,135,104 Effective search space: 90914587776 Effective search space used: 90914587776 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)