BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0341.0001.p (276 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_389659.1| similar to spore germination protein [Bacillus ... 135 1e-30 ref|NP_832854.1| Spore germination protein BB [Bacillus cereus A... 131 1e-29 ref|NP_391462.1| gerBB [Bacillus subtilis] >gi|729628|sp|P39570|... 112 7e-24 ref|NP_391186.1| gerAB [Bacillus subtilis] >gi|1708033|sp|P07869... 105 1e-21 ref|NP_691616.1| spore germination protein [Oceanobacillus iheye... 87 3e-16 >ref|NP_389659.1| similar to spore germination protein [Bacillus subtilis] pir||E69889 spore germination protein homolog yndE - Bacillus subtilis emb|CAB13660.1| yndE [Bacillus subtilis subsp. subtilis str. 168] Length = 363 Score = 135 bits (340), Expect = 1e-30, Method: Compositional matrix adjust. Identities = 81/263 (30%), Positives = 122/263 (46%) Query: 3 AVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVITGK 62 A +++LPRT+ EQTQ+PD W +LL + V I KL Q++P +TFYE + I GK Sbjct: 23 AAGVLTLPRTVTEQTQSPDGWISVLLGGVLAVIAGMIIAKLSQQYPKETFYEYSRHIVGK 82 Query: 63 FISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGLHA 122 ++ LI+I F+ Y + + + R+M+E + LE TP I + GL Sbjct: 83 WLGHLISIVFITYFLALGAFEVRVMSEIVDFFLLEGTPSWAIIMTVLWIGLYSITQGLDP 142 Query: 123 MVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYESM 182 + RL E ++LRPV G P+ + +K + E M Sbjct: 143 IARLFEMIFPITVIIFLTIALMSLGIFEINNLRPVLGDGIMPVLRGVKTTNLSFTCSEIM 202 Query: 183 LILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQIEF 242 IL FM P+ A KA IG + + Y++ + MVIG +SVE V WP + + E Sbjct: 203 FILVAFMKKPKNAVKAVVIGTGVVTSFYMITMIMVIGALSVEGVVTRTWPGLDLMRSFEI 262 Query: 243 PGSXXXXXXXXXXXXWTIKIFMT 265 PG W +++F T Sbjct: 263 PGLIFERFESFLLVIWIMQLFAT 285 >ref|NP_832854.1| Spore germination protein BB [Bacillus cereus ATCC 14579] emb|CAB69788.1| putative spore germination protein [Bacillus cereus] emb|CAB96937.1| yndE [Bacillus cereus] gb|AAP10055.1| Spore germination protein BB [Bacillus cereus ATCC 14579] Length = 363 Score = 131 bits (330), Expect = 1e-29, Method: Compositional matrix adjust. Identities = 82/265 (30%), Positives = 120/265 (45%) Query: 1 VYAVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVIT 60 + I++LPR E+ +TPDVW ++L + + I VKL Q+FP KTFY+ + I Sbjct: 22 ILGTGILTLPRASVEKVKTPDVWLSVILGGILAMVSGVIMVKLSQQFPEKTFYQYSQDIV 81 Query: 61 GKFISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGL 120 GK+I L++ + Y + + R MAE + LE TP I GG+ Sbjct: 82 GKWIGRLLSFLIIGYFLTTSAFQIRSMAEVISFFLLEGTPTWAIVMPFMWIGLYLIMGGI 141 Query: 121 HAMVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYE 180 +++ R+ E D+LRPV G P+ +K A G E Sbjct: 142 NSIARMFEIIFPITVFIFLIISFMSIGIFEIDNLRPVLGLGIKPVLDGIKTTSLAYTGPE 201 Query: 181 SMLILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQI 240 MLIL FM +KA KA +G SI + YV+ V MVIG +S++ V WP + + Sbjct: 202 IMLILLVFMEQRKKAVKAILVGISIPLIFYVITVVMVIGALSIDGVVTRTWPTIDLMRSF 261 Query: 241 EFPGSXXXXXXXXXXXXWTIKIFMT 265 E G W ++IF T Sbjct: 262 EISGLIFERFESLLLVIWIMQIFAT 286 >ref|NP_391462.1| gerBB [Bacillus subtilis] sp|P39570|GRBB_BACSU Spore germination protein B2 pir||I39856 spore germination apparatus protein gerBB - Bacillus subtilis gb|AAA22467.1| gerB emb|CAB15598.1| probable component of a germinant receptor [Bacillus subtilis subsp. subtilis str. 168] Length = 368 Score = 112 bits (281), Expect = 7e-24, Method: Compositional matrix adjust. Identities = 66/261 (25%), Positives = 118/261 (45%), Gaps = 1/261 (0%) Query: 3 AVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVITGK 62 +++LPR+ AE T +P W +LL + + +V + + Q+ GKT ++LNS++ GK Sbjct: 23 GAGVLTLPRSAAE-TGSPSGWLMILLQGVIFIIIVLLFLPFLQKNSGKTLFKLNSIVAGK 81 Query: 63 FISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGLHA 122 FI L+N+ +Y + I R++ E + L+ T ++++ GG+++ Sbjct: 82 FIGFLLNLYICLYFIGIVCFQARILGEVVGFFLLKNTRMAVVVFIFLAVAIYHVGGGVYS 141 Query: 123 MVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYESM 182 + ++ D +RPVF G+ F G+E + Sbjct: 142 IAKVYAYIFPITLIIFMMLLMFSFRLFQLDFIRPVFEGGYQSFFSLFPKTLLYFSGFEII 201 Query: 183 LILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQIEF 242 L FM +P++ KA A+G + + Y + + +VIGCM+V E + WP +S +E Sbjct: 202 FYLVPFMRDPKQVKKAVALGIATSTLFYSITLLIVIGCMTVAEAKTVTWPTISLIHALEV 261 Query: 243 PGSXXXXXXXXXXXXWTIKIF 263 PG WT + F Sbjct: 262 PGIFIERFDLFLQLTWTAQQF 282 >ref|NP_391186.1| gerAB [Bacillus subtilis] sp|P07869|GRAB_BACSU Spore germination protein A2 pir||F69629 spore germination protein II - Bacillus subtilis gb|AAA92781.1| formerly called gene II emb|CAB15296.1| gerAB [Bacillus subtilis subsp. subtilis str. 168] emb|CAA11747.1| GerAB protein [Bacillus subtilis] Length = 365 Score = 105 bits (262), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 71/263 (26%), Positives = 114/263 (43%) Query: 3 AVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVITGK 62 +++LPR + + TPD W L+L + +++N + ++ + +E GK Sbjct: 25 GAGLLTLPRALTTKANTPDGWITLILEGFIFIFFIYLNTLIQKKHQYPSLFEYLKEGLGK 84 Query: 63 FISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGLHA 122 +I +I + Y + + S R MAE +K LE+TP+ +I GGL Sbjct: 85 WIGSIIGLLICGYFLGVASFETRAMAEMVKFFLLERTPIQVIILTFICCGIYLMVGGLSD 144 Query: 123 MVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYESM 182 + RL ++LRPV +G GPI SL VV + LG E M Sbjct: 145 VSRLFPFYLTVTIIILLIVFGISFKIFDINNLRPVLGEGLGPIANSLTVVSISFLGMEVM 204 Query: 183 LILTNFMVNPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFAQQIEF 242 L L M + ++ ++G+ I + LY+L +V+G ++ EV L WP +S Q E Sbjct: 205 LFLPEHMKKKKYTFRYASLGFLIPIILYILTYIIVVGALTAPEVKTLIWPTISLFQSFEL 264 Query: 243 PGSXXXXXXXXXXXXWTIKIFMT 265 G W I+ F T Sbjct: 265 KGIFIERFESFLLVVWIIQFFTT 287 >ref|NP_691616.1| spore germination protein [Oceanobacillus iheyensis HTE831] dbj|BAC12651.1| spore germination protein [Oceanobacillus iheyensis HTE831] Length = 370 Score = 87.4 bits (215), Expect = 3e-16, Method: Compositional matrix adjust. Identities = 59/247 (23%), Positives = 104/247 (42%), Gaps = 6/247 (2%) Query: 1 VYAVAIVSLPRTIAEQTQTPDVWQGLLLASLFGVGVVFINVKLCQRFPGKTFYELNSVIT 60 V AV I++ PR +A +T+ D W LL A + + + ++ + +FP ++F + S + Sbjct: 24 VVAVGILTYPRLLASKTEAADGWVTLLTAGILSIMITWVIANIASKFPNQSFLDYASFLL 83 Query: 61 GKFISLLINIAFMVYCVMICSLVCRMMAEFLKALALEKTPVSMIXXXXXXXXXXXTSGGL 120 + +++ I++ +++ C I +A+ + ++TP ++ SG Sbjct: 84 SRPVAIAISVLYIIVCWFIVGYELAFIADISQHYLFDRTPTEVVVLAFLLVIVYAVSGSR 143 Query: 121 HAMVRLIEXXXXXXXXXXXXXXXXXXXXXXXDHLRPVFHQGWGPIFKSLKVVPFATLGYE 180 +AM R+ DHL PVF W K L P L Y Sbjct: 144 NAMFRMCFGFMAFSIIATIVLVFMSLPFGDFDHLLPVFTTDWKTYIKEL---PNGILSYT 200 Query: 181 SMLILTNFMV---NPQKAWKAGAIGYSIAMALYVLMVTMVIGCMSVEEVSRLQWPVVSFA 237 IL +M +P+K K+ AIG S+ + LYV + I + + +P+V A Sbjct: 201 GGFILLFYMKLNRDPKKGPKSAAIGVSVVVILYVSIYLACIAVLGNVTTANTIYPLVELA 260 Query: 238 QQIEFPG 244 I G Sbjct: 261 NTIHSAG 267 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.330 0.140 0.434 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 286,189,083 Number of extensions: 8730641 Number of successful extensions: 27261 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 27251 Number of HSP's successfully gapped: 22 Length of query: 276 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 152 Effective length of database: 420,565,302 Effective search space: 63925925904 Effective search space used: 63925925904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.9 bits) S2: 125 (52.8 bits)