BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0043.p (389 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAB96938.1| yndF [Bacillus cereus] 286 8e-76 ref|NP_832853.1| Spore germination protein BC [Bacillus cereus A... 285 1e-75 ref|NP_389660.1| similar to spore germination protein [Bacillus ... 281 3e-74 ref|NP_391463.1| gerBC [Bacillus subtilis] >gi|729630|sp|P39571|... 174 4e-42 ref|NP_391187.1| gerAC [Bacillus subtilis] >gi|7404371|sp|P07870... 129 1e-28 >emb|CAB96938.1| yndF [Bacillus cereus] Length = 390 Score = 286 bits (731), Expect = 8e-76, Method: Compositional matrix adjust. Identities = 142/378 (37%), Positives = 235/378 (62%), Gaps = 10/378 (2%) Query: 10 TGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIVPKQSYGNS 69 TGCWSS E+E+LS+ + +D G+ + E+ E E G Y K N IT T +IV Q+ Sbjct: 17 TGCWSSKEVEELSLTAGIALDKGKDSTIEK--EDEEGGYPKRNIITATYQIVSSQAQSKG 74 Query: 70 NKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLLQEQRIQD 129 N + Y+N+SETGDS+ +I+R+F+++ + + HLK IVIS L++ ++ Sbjct: 75 N------GQQKRYINVSETGDSIHQIVREFSLKRESTMFSPHLKNIVISDSLVRTYSLEQ 128 Query: 130 LMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRNSRIMKGIIT 189 L++ LRDN++R SC++L+S+G A + L++ GEIPAF L G+ NR R +RI+ + Sbjct: 129 LLEQYLRDNEVRLSCMLLISKGLAKEVLESNKKGEIPAFRLSGIADNRNRTTRIVPPVSL 188 Query: 190 SELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIESIAWIKGDVK 249 ++L+ M + SFLLQN++ E +F+G VIKG T + +G L +Q++E + WI G Sbjct: 189 AKLEGKMRSGSSFLLQNVISIKGETKFAGAAVIKGKTKKLMGFLNEQELEGVVWINGKRN 248 Query: 250 GGTLKTYNPDHQT-VTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKWKDPGLPSK 308 GG LKT++ + + + YE VN ++ F V+IESEGRL E W G + Sbjct: 249 GGVLKTFDKESKKLIIYEIKSIKSKIIPHVNGNN-ISFDVNIESEGRLSENWIQSGKDFE 307 Query: 309 KEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKLKDHWDETF 368 E+L++ + ++ + ++ + K+Q E++V++AGFG++L ++ P+ W+K+K +WD+TF Sbjct: 308 NEFLKREEKAIEKEVKHLVHHVTKKIQKEYQVDIAGFGNQLRIKHPRTWEKVKKNWDQTF 367 Query: 369 SRTPVTFDIKLTITDYGS 386 S+ P+ + I +TI DYG+ Sbjct: 368 SKVPIKYHINVTIQDYGA 385 >ref|NP_832853.1| Spore germination protein BC [Bacillus cereus ATCC 14579] emb|CAB69789.1| putative spore germination protein [Bacillus cereus] gb|AAP10054.1| Spore germination protein BC [Bacillus cereus ATCC 14579] Length = 397 Score = 285 bits (729), Expect = 1e-75, Method: Compositional matrix adjust. Identities = 142/378 (37%), Positives = 235/378 (62%), Gaps = 10/378 (2%) Query: 10 TGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIVPKQSYGNS 69 TGCWSS E+E+LS+ + +D G+ + E+ E E G Y K N IT T +IV Q+ Sbjct: 24 TGCWSSKEVEELSLTAGIALDKGKDSTIEK--EDEEGGYPKRNIITATYQIVSSQAQSKG 81 Query: 70 NKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLLQEQRIQD 129 N + Y+N+SETGDS+ +I+R+F+++ + + HLK IVIS L++ ++ Sbjct: 82 N------GQQKRYINVSETGDSIHQIVREFSLKRESTMFSPHLKNIVISDSLVRTYSLEQ 135 Query: 130 LMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRNSRIMKGIIT 189 L++ LRDN++R SC++L+S+G A + L++ GEIPAF L G+ NR R +RI+ + Sbjct: 136 LLEQYLRDNEVRLSCMLLISKGLAKEVLESNKKGEIPAFRLSGIADNRNRTTRIVPPVSL 195 Query: 190 SELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIESIAWIKGDVK 249 ++L+ M + SFLLQN++ E +F+G VIKG T + +G L +Q++E + WI G Sbjct: 196 AKLEGKMRSGSSFLLQNVISIKGETKFAGAAVIKGKTKKLMGFLNEQELEGVVWINGKRN 255 Query: 250 GGTLKTYNPDHQT-VTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKWKDPGLPSK 308 GG LKT++ + + + YE VN ++ F V+IESEGRL E W G + Sbjct: 256 GGVLKTFDKESKKLIIYEIKSIKSKIIPHVNGNN-ISFDVNIESEGRLSENWIQSGKDFE 314 Query: 309 KEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKLKDHWDETF 368 E+L++ + ++ + ++ + K+Q E++V++AGFG++L ++ P+ W+K+K +WD+TF Sbjct: 315 NEFLKREEKAIEKEVKHLVHHVTKKIQKEYQVDIAGFGNQLRIKHPRTWEKVKKNWDQTF 374 Query: 369 SRTPVTFDIKLTITDYGS 386 S+ P+ + I +TI DYG+ Sbjct: 375 SKVPIKYHINVTIQDYGA 392 >ref|NP_389660.1| similar to spore germination protein [Bacillus subtilis] pir||F69889 spore germination protein homolog yndF - Bacillus subtilis emb|CAB13661.1| yndF [Bacillus subtilis subsp. subtilis str. 168] Length = 404 Score = 281 bits (718), Expect = 3e-74, Method: Compositional matrix adjust. Identities = 146/389 (37%), Positives = 236/389 (60%), Gaps = 5/389 (1%) Query: 1 LAAALLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEI 60 + L++ TGCWSS EIEDL + A+ ID G+ T TE+ ++EGGSY K + IT+T + Sbjct: 14 IVCLLMICVTGCWSSREIEDLGLTFAIAIDKGKETNTEKELKEEGGSYPKKDNITLTYQF 73 Query: 61 VPKQSYGNSNKTPDTGAKA--PNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVIS 118 V +++ G + Y+NISETGDS+ +I + A+R DR V HLKV+V+S Sbjct: 74 VNEKAAGAGTSGGGGSGQGAQKAYINISETGDSLQQIGSEVALRRDREVFSPHLKVVVMS 133 Query: 119 RQLLQEQRIQDLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRF 178 +L I +++D RDN+IR SCL+L ++G A D L+ K GEIPAF L G+ N Sbjct: 134 EDVLHTFPIDEMLDQFFRDNEIRLSCLVLSAKGEARDALQLKENGEIPAFRLIGLGENEH 193 Query: 179 RNSRIMKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDI 238 + SRI+ + ++L +H+ SFLLQN+V AN V++SG VI G + + IG L + + Sbjct: 194 KVSRILPPMTLAKLIGKLHSGSSFLLQNVVAANGAVKYSGAAVINGKSKKMIGTLNEYET 253 Query: 239 ESIAWIKGDVKGGTLKTYN-PDHQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLI 297 E I WI+G+ KGG +K+++ QT+ Y+ V K + FHV IESEG L+ Sbjct: 254 EGITWIRGEGKGGVVKSHDKKSQQTLAYDINKIKSRIQPIV-KGKDISFHVDIESEGDLV 312 Query: 298 EKWKDPGLPSKKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEW 357 E W + +++++++ + + +++ ++ K+Q ++K +VAGF + + P W Sbjct: 313 ENW-NTKEALDTQFIDRLETTIENEVKKIVGQVLKKIQHDYKADVAGFDESFRLTYPHLW 371 Query: 358 KKLKDHWDETFSRTPVTFDIKLTITDYGS 386 K++K++WD+TFS+ +T+ + +TIT +G+ Sbjct: 372 KRVKNNWDDTFSKADITYSVNVTITHFGT 400 >ref|NP_391463.1| gerBC [Bacillus subtilis] sp|P39571|GRBC_BACSU Spore germination protein B3 precursor pir||I39857 spore germination apparatus protein gerBC - Bacillus subtilis gb|AAA22468.1| gerB emb|CAB15599.1| gerBC [Bacillus subtilis subsp. subtilis str. 168] Length = 374 Score = 174 bits (440), Expect = 4e-42, Method: Compositional matrix adjust. Identities = 99/386 (25%), Positives = 191/386 (49%), Gaps = 31/386 (8%) Query: 5 LLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIVPKQ 64 +LL GCW +IE LS L ID Q + T++ ++P+ Sbjct: 13 MLLALCGCWDVKDIEQLSFARGLAIDETNDHQYKLTYQ----------------NLLPQ- 55 Query: 65 SYGNSNKTPDTGAKA-PNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLLQ 123 + D+ A P +VN++ G ++LE + +I+ D PV HLKVI++ +L++ Sbjct: 56 -------SEDSQASGKPEFVNVTSHGKTILEAVSDVSIK-DPPVYSDHLKVILLGEKLMR 107 Query: 124 EQRIQDLMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGE---IPAFHLRGMTRNRFRN 180 Q + +++ +RD+++R S ++ + G AAD + +P+ L +T + N Sbjct: 108 NQNVDQVLNHFIRDDELRRSSYLMAARGNAADVFTKGNPNQQQPMPSEKLIDLTTHSGYN 167 Query: 181 SRIMKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIES 240 +IM + S+L+Q + + ++ G G+IK + + +G L + ++ Sbjct: 168 GKIMIPLRIGRASVYSQNGYSYLIQAVKNEKGKAKYDGAGIIKRGSNKLVGFLSADETQT 227 Query: 241 IAWIKGDVKGGTLKTYNPDHQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKW 300 ++W+ G ++GG + T + H +T+E + ++ +P FH+S++++G L E Sbjct: 228 LSWVMGTIQGGVMPTTDKGH-PITFEIKKSKTKIKPVI-ENGKPVFHISVKTKGILTEDQ 285 Query: 301 KDPGLPSKKEYLEKMQGIFKERLTEMIQSLMHKMQSEFKVEVAGFGDRLSVEEPQEWKKL 360 K YL +++ IF+++L ++ +M K+Q E+K + D + ++ P W K+ Sbjct: 286 NPNENSFSKSYLHRLENIFEKKLERDVKQVMDKLQHEYKTDPVFLSDHIRIQHPDYWNKV 345 Query: 361 KDHWDETFSRTPVTFDIKLTITDYGS 386 K HWDE FS T +DI I ++G+ Sbjct: 346 KGHWDEIFSETDFKYDISFKIINFGT 371 >ref|NP_391187.1| gerAC [Bacillus subtilis] sp|P07870|GRAC_BACSU Spore germination protein A3 precursor pir||G69629 germination response to L-alanine gerAC - Bacillus subtilis emb|CAB15297.1| gerAC [Bacillus subtilis subsp. subtilis str. 168] emb|CAA11746.1| GerAC protein [Bacillus subtilis] Length = 373 Score = 129 bits (323), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 96/387 (24%), Positives = 182/387 (47%), Gaps = 32/387 (8%) Query: 3 AALLLTETGCWSSNEIEDLSMYTALTIDAGQPTQTEQTFEKEGGSYLKDNKITVTIEIVP 62 LLL+ GCW S IE+LS+ + +D E T + +VP Sbjct: 11 CTLLLS--GCWDSENIEELSLVIGIGLDKPDDENLELTQQI----------------LVP 52 Query: 63 KQSYGNSNKTPDTGAKAPNYVNISETGDSVLEILRQFAIRLDRPVIGHHLKVIVISRQLL 122 K + D P ++I++ G +V +++R A++ +P HL++I++S+ ++ Sbjct: 53 KIISAKEGSSSD-----PTQLSITK-GKTVHQMMRTSALK-HKPTFSQHLRLILLSKSVI 105 Query: 123 QEQRIQD-LMDFVLRDNDIRPSCLILMSEGRAADTLKTKFGGEIPAFHLRGMTRNRFRNS 181 +Q D +++ +RDN R S + ++ GR D GE + + +T N Sbjct: 106 ADQIGMDAIINQFVRDNGTRRSSYVFITNGRTKDIFNMNDEGEPASNVIYDLTENNKVTI 165 Query: 182 RIMKGIITSELDALMHAKKSFLLQNIVEANQEVEFSGGGVIKGDTGRWIGNLEQQDIESI 241 R M+ + E+ + + SFL+ ++ + N ++ +G +IK W +L ++++I Sbjct: 166 RTMEPVTLGEISEHLTSDDSFLIPHVGKENGKLAINGASIIKNKL--WHRDLTPIEVQNI 223 Query: 242 AWIKGDVKGGTLKTYNPDHQTVTYEXXXXXXXXXXXVNKDHEPEFHVSIESEGRLIEKWK 301 + G V+GG + H +YE KD + +F V+ EGRL E W Sbjct: 224 SLFSGTVEGGVIDLKRDGH-LFSYEVYSSNRKIKTAY-KDGKFKFTVTRNIEGRLSEDWN 281 Query: 302 DPGLPSKKEYLEKMQGIFKERLTEMIQSLM-HKMQSEFKVEVAGFGDRLSVEEPQEWKKL 360 K Y++ ++ ++R+ E + S + K+Q E K +V G G+ + + PQ+WKK+ Sbjct: 282 PNEDSFKDSYIKSIEKTVEKRVHETVTSFITEKLQKEIKADVTGLGNEVRIHYPQKWKKI 341 Query: 361 KDHWDET-FSRTPVTFDIKLTITDYGS 386 WD+ FS + + + + + D+G+ Sbjct: 342 SRKWDDDYFSNAEIDYRVNVIVRDFGT 368 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.134 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 575,803,313 Number of extensions: 23445439 Number of successful extensions: 59327 Number of sequences better than 1.0e-05: 34 Number of HSP's gapped: 59268 Number of HSP's successfully gapped: 35 Length of query: 389 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 262 Effective length of database: 412,420,005 Effective search space: 108054041310 Effective search space used: 108054041310 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)