BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0340.0004.p (141 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_832097.1| hypothetical Membrane Spanning Protein [Bacillu... 93 2e-18 ref|ZP_00183700.1| hypothetical protein Exigu091501 [Exiguobacte... 86 4e-16 ref|NP_563525.1| hypothetical protein [Clostridium perfringens] ... 80 2e-14 ref|NP_350132.1| Uncharacterized conserved membrane protein [Clo... 76 2e-13 ref|NP_780855.1| conserved membrane protein [Clostridium tetani ... 64 7e-10 >ref|NP_832097.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] gb|AAP09298.1| hypothetical Membrane Spanning Protein [Bacillus cereus ATCC 14579] Length = 141 Score = 93.2 bits (230), Expect = 2e-18, Method: Compositional matrix adjust. Identities = 49/128 (38%), Positives = 72/128 (56%) Query: 14 ITLQGVANARISQDIGTWQAATITQFTGFVMALMILLFVRDAKKQGFKQVSPLYLFGGAL 73 ITLQG NA++S IG W + IT GF++A + L ++ K K V +YL GA Sbjct: 14 ITLQGTFNAKLSSHIGIWSTSIITHLIGFIIATTVFLLKKEEKVTDLKSVKKIYLVAGAF 73 Query: 74 AAFIIFSEVTAIQGIGVTLTISALLIAQLCLTFLIDIKGWFGVVKQKMRLPQFIGIGMMV 133 FII +E AI +GVTLT L+ AQL +I++KG F + K +M +G +M+ Sbjct: 74 GGFIICAETMAIYSLGVTLTAGTLMAAQLLTATVIEMKGLFHIKKIQMERYHIVGTIVMI 133 Query: 134 AGVVILKL 141 G+V+ + Sbjct: 134 IGIVVFNM 141 >ref|ZP_00183700.1| hypothetical protein Exigu091501 [Exiguobacterium sp. 255-15] Length = 147 Score = 85.5 bits (210), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 49/127 (38%), Positives = 77/127 (60%) Query: 14 ITLQGVANARISQDIGTWQAATITQFTGFVMALMILLFVRDAKKQGFKQVSPLYLFGGAL 73 I+LQ V NAR+S G W T+ F G + +L+ L+F R G +V PLYLFGG + Sbjct: 3 ISLQTVLNARMSHAFGAWATTTLVFFVGLLGSLLALIFFRGGTLAGLAEVEPLYLFGGFV 62 Query: 74 AAFIIFSEVTAIQGIGVTLTISALLIAQLCLTFLIDIKGWFGVVKQKMRLPQFIGIGMMV 133 ++F + IQ +G ++ IS +LI+QL +D+ GWFG+ K ++ Q IG+ +M+ Sbjct: 63 GVGVVFCVMRGIQLLGPSIAISVVLISQLSFALCVDVFGWFGLPKVELSFGQIIGLAVML 122 Query: 134 AGVVILK 140 +G+ +LK Sbjct: 123 SGIFVLK 129 >ref|NP_563525.1| hypothetical protein [Clostridium perfringens] dbj|BAB82315.1| hypothetical protein [Clostridium perfringens str. 13] Length = 143 Score = 80.1 bits (196), Expect = 2e-14, Method: Compositional matrix adjust. Identities = 40/116 (34%), Positives = 67/116 (57%) Query: 13 CITLQGVANARISQDIGTWQAATITQFTGFVMALMILLFVRDAKKQGFKQVSPLYLFGGA 72 +++QGV N R+S+ IG W+ TI Q + FV+AL+I+L +R+ + V+ +YL GGA Sbjct: 13 AMSIQGVFNTRVSEKIGVWETNTIVQGSAFVLALLIMLMLRNGNYSELRSVNKIYLLGGA 72 Query: 73 LAAFIIFSEVTAIQGIGVTLTISALLIAQLCLTFLIDIKGWFGVVKQKMRLPQFIG 128 + I ++ + A+ +G T IS +LI+QL +ID F K + ++G Sbjct: 73 IGVVITYTVMAAVGSLGPTFGISIILISQLLTAGIIDAFALFDSEKIRFAAHNYLG 128 >ref|NP_350132.1| Uncharacterized conserved membrane protein [Clostridium acetobutylicum] pir||E97335 uncharacterized conserved membrane protein CAC3547 [imported] - Clostridium acetobutylicum gb|AAK81472.1| Uncharacterized conserved membrane protein [Clostridium acetobutylicum] Length = 143 Score = 75.9 bits (185), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 44/129 (34%), Positives = 70/129 (54%) Query: 13 CITLQGVANARISQDIGTWQAATITQFTGFVMALMILLFVRDAKKQGFKQVSPLYLFGGA 72 C++LQGV N R+S+ IG W+ Q T ++ L+I + + + LYL GG Sbjct: 13 CMSLQGVFNTRLSEKIGLWETNAWVQGTALLVTLVICFIYGNGNLKMIGTANKLYLTGGI 72 Query: 73 LAAFIIFSEVTAIQGIGVTLTISALLIAQLCLTFLIDIKGWFGVVKQKMRLPQFIGIGMM 132 L A IIF+ + + +G T +IS +LIAQL +ID G F + + IG+ +M Sbjct: 73 LGAIIIFTVMKGVSSLGPTCSISIILIAQLLSAAIIDFFGLFHTDRLTFDSTKIIGVVLM 132 Query: 133 VAGVVILKL 141 +AG++ K+ Sbjct: 133 LAGIICFKI 141 >ref|NP_780855.1| conserved membrane protein [Clostridium tetani E88] gb|AAO34792.1| conserved membrane protein [Clostridium tetani E88] Length = 155 Score = 64.3 bits (155), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 40/103 (38%), Positives = 59/103 (57%) Query: 13 CITLQGVANARISQDIGTWQAATITQFTGFVMALMILLFVRDAKKQGFKQVSPLYLFGGA 72 ++ QGV N R+S +G W+A + Q G ++ L+ LL + D + K+V+ LYL GG Sbjct: 24 SMSFQGVFNTRLSDKVGLWEANFLVQSIGAILTLIALLLLGDGNIKKIKEVNKLYLSGGL 83 Query: 73 LAAFIIFSEVTAIQGIGVTLTISALLIAQLCLTFLIDIKGWFG 115 L IIF+ + I+ +G T IS +L+AQL LID FG Sbjct: 84 LGVIIIFTVMKGIKSLGPTYAISTILVAQLLSAALIDAFALFG 126 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.332 0.144 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 155,092,350 Number of extensions: 5207966 Number of successful extensions: 17727 Number of sequences better than 1.0e-05: 21 Number of HSP's gapped: 17731 Number of HSP's successfully gapped: 22 Length of query: 141 Length of database: 757,237,578 Length adjustment: 102 Effective length of query: 39 Effective length of database: 480,297,480 Effective search space: 18731601720 Effective search space used: 18731601720 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (22.0 bits) S2: 120 (50.8 bits)