BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0337.0014.p (359 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_979888.1| spore germination protein [Bacillus cereus ATCC... 80 9e-14 ref|NP_833307.1| Spore germination protein SB [Bacillus cereus A... 80 1e-13 ref|NP_657483.1| hypothetical protein predicted by GeneMark [Bac... 80 1e-13 ref|NP_243053.1| BH2187~unknown conserved protein in bacilli [Ba... 72 1e-11 ref|NP_981168.1| spore germination protein GerHB [Bacillus cereu... 67 7e-10 >ref|NP_979888.1| spore germination protein [Bacillus cereus ATCC 10987] gb|AAS42496.1| spore germination protein [Bacillus cereus ATCC 10987] Length = 365 Score = 79.7 bits (195), Expect = 9e-14, Method: Compositional matrix adjust. Identities = 73/325 (22%), Positives = 130/325 (40%), Gaps = 14/325 (4%) Query: 3 EKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRL 62 ++S F + + Q+G V +LPR++A T+GW T+ I + Sbjct: 7 REISLFQYILTISGVQVGFGVLTLPREVAQGANTDGWMSIIIGCAITTLVSLSIVKIMEK 66 Query: 63 GDGKPVFEIMERSIPK------VILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTN 116 G + +++ R + K +I + +Y L A+ LIF + YV+ + P T Sbjct: 67 HPGYTLLDVLTRYLGKWLGRAGMIFWILYAVLAAISLIFSLL----YVI--HIWILPRTP 120 Query: 117 PMXXXXXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQG 176 KG+ IS+ A + T WM LL W+ L P + +G Sbjct: 121 MFLIMILLSIPMLMLACKGVLIISRYAVFTVFFTLWMPLLLFIPLKDGHWIYLLPLLKEG 180 Query: 177 GHDQIKGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFF 236 + +LG+E + +L+PY++ + I N +Y+ ++ V F +F Sbjct: 181 WIPVLNTVKSTIIAFLGFEFAFILYPYLNNKSSAKKGIVIANLITLFLYLQVTFVSFVYF 240 Query: 237 SFGQXXXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPK 296 S F F+ER E + F+LF+I + Y + A + + +++ K Sbjct: 241 SPDGITKFLWPTLSLVTPFHFSFLERFEIIFLSFYLFIIFDSCIPYIFTASDGINQLLNK 300 Query: 297 L--KIPVMVFLLTAIAYCISYIPKT 319 +P+ L I YIP + Sbjct: 301 KGSSLPIWFLLFGCIVVLFFYIPSS 325 >ref|NP_833307.1| Spore germination protein SB [Bacillus cereus ATCC 14579] gb|AAP10508.1| Spore germination protein SB [Bacillus cereus ATCC 14579] Length = 365 Score = 79.7 bits (195), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 75/325 (23%), Positives = 129/325 (39%), Gaps = 14/325 (4%) Query: 3 EKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRL 62 ++S F + + Q+G V +LPR++A T+GW T+ I + Sbjct: 7 REISLFQYILTISGVQVGFGVLTLPREVAQGANTDGWMSIIIGCALTTLVSLCIVKIMEK 66 Query: 63 GDGKPVFEIMERSIPK------VILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTN 116 + +++ R + K +I + +Y L A+ LIF + YV+ + P T Sbjct: 67 HPEYTLLDVLTRYLGKWLGRVGMIFWILYAVLAAISLIFSLL----YVI--HIWILPRTP 120 Query: 117 PMXXXXXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQG 176 KG+ IS+ A + T WM FLL W+ L P + +G Sbjct: 121 MFLIMILLSIPMLMLACKGVLIISRYAVFTVFFTLWMPFLLFIPLKDGHWIYLLPLLKEG 180 Query: 177 GHDQIKGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFF 236 + +LG+E + +L+PY+ + I N IY+ ++ V F +F Sbjct: 181 WIPVLNTVKSTIIAFLGFEFAFVLYPYLSNKSSAKKGIVLANLITLFIYLQVTFVSFVYF 240 Query: 237 SFGQXXXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPK 296 S F F+ER E + F+LF+I + Y + A + + + + K Sbjct: 241 SPDGITKFLWPTLSLVTPFHFSFLERFEIIFLSFYLFIIFDSCIPYIFTASDGINQFLNK 300 Query: 297 L--KIPVMVFLLTAIAYCISYIPKT 319 +P+ V L I YIP + Sbjct: 301 KGSSLPIYVLLFGCIFILFFYIPSS 325 >ref|NP_657483.1| hypothetical protein predicted by GeneMark [Bacillus anthracis A2012] ref|NP_845902.1| spore germination protein [Bacillus anthracis str. Ames] gb|AAP27388.1| spore germination protein [Bacillus anthracis str. Ames] Length = 365 Score = 79.7 bits (195), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 74/325 (22%), Positives = 130/325 (40%), Gaps = 14/325 (4%) Query: 3 EKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRL 62 ++S F + + Q+G V +LPR++A T+GW T+ I + Sbjct: 7 REISLFQYILTISGVQVGFGVLTLPREVAQGANTDGWMSIIIGCVITTLVSLCIVKIMEK 66 Query: 63 GDGKPVFEIMERSIPK------VILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTN 116 G + +++ R + K +I + +Y L AV L+F + YV+ + P + Sbjct: 67 HPGYTLLDVLTRYLGKWLGRIGMIFWILYAVLAAVSLVFSLL----YVI--HIWILPRSP 120 Query: 117 PMXXXXXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQG 176 KG+ IS+ A T WM LL W+ L PF+ +G Sbjct: 121 MFLIMILLSIPMLMLACKGVLIISRFAVFTVIFTLWMPLLLFIPLKDGHWIYLLPFLKEG 180 Query: 177 GHDQIKGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFF 236 + +LG+E + +L+PY+ + I N +Y+ ++ V F +F Sbjct: 181 WIPVVTTVKSTIIAFLGFEFAFVLYPYLTNKPAAKKGIIIANMITLCVYLQVTFVSFVYF 240 Query: 237 SFGQXXXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPK 296 S F F+ER E + F+LF+I + Y + A + + +++ K Sbjct: 241 SPDGITKFLWPTLSLVTPFHFSFLERFEIIFLSFYLFIIFDSCIPYIFTASDGINQLLNK 300 Query: 297 L--KIPVMVFLLTAIAYCISYIPKT 319 +PV + L I YIP + Sbjct: 301 KGSPLPVWILLFGCIFVLFFYIPSS 325 >ref|NP_243053.1| BH2187~unknown conserved protein in bacilli [Bacillus halodurans] pir||C83923 hypothetical protein BH2187 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05906.1| BH2187~unknown conserved protein in bacilli [Bacillus halodurans] Length = 358 Score = 72.4 bits (176), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 70/337 (20%), Positives = 140/337 (41%), Gaps = 1/337 (0%) Query: 2 MEKLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYR 61 ME LS +L+ M Q+G+ + SLP +A GW L + + Sbjct: 1 MEYLSVKQTVVLIVMIQVGVGILSLPASVAAEANNLGWVSVLLAGVIVQLAAVLYHISLK 60 Query: 62 LGDGKPVFEIMERSIPKVILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTNPMXXX 121 VF ME+ I +++ + + L +L+ ++ + +V L + + T + Sbjct: 61 NIQEPTVFLFMEKRIGRLLTGLLVVALAIYFLLTSGILLRSFVELLHIWVYTETPRIMLV 120 Query: 122 XXXXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQGGHDQI 181 I ++S ++ +L ++ +LL+ ++V L P D + Sbjct: 121 LLIFIPIVYLATGSITSLSNVSSFIIFLVIFLLSMLLFHLKQAQFVLLDPASGITMMDLL 180 Query: 182 KGAFDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFFSFGQX 241 +G+ +G+EL I Y+ + K++L + N Y ++ + +S + Sbjct: 181 RGSLQTSLALIGFELIIFFHFYLKEKKRTLIVLTVSNWITVSCYTFVTFIAMLTYSAEKL 240 Query: 242 XXXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPKLKIPV 301 I+ ++RLE + + L+LIT +Y W ++++R I+PK ++ Sbjct: 241 EKEVWPTLSLYKLIELHIIQRLEFFIVMVWTPLMLITTGLYLWAFIKSIRYILPK-RLNR 299 Query: 302 MVFLLTAIAYCISYIPKTLDQVNNWILHLGYAAIGIA 338 + L+ I+ IPKT +V + +GY I I+ Sbjct: 300 LCVSLSLAVPVIALIPKTQPEVLTYSNEIGYIGIVIS 336 >ref|NP_981168.1| spore germination protein GerHB [Bacillus cereus ATCC 10987] gb|AAS43776.1| spore germination protein GerHB [Bacillus cereus ATCC 10987] Length = 366 Score = 67.0 bits (162), Expect = 7e-10, Method: Compositional matrix adjust. Identities = 70/325 (21%), Positives = 132/325 (40%), Gaps = 4/325 (1%) Query: 4 KLSPFHVAILVYMTQIGIVVFSLPRQLADYFGTNGWXXXXXXXXXTSFNIYLITLVYRLG 63 ++SP V L++ Q G V R A G +GW I+++ + + Sbjct: 9 QISPIFVFFLIHGAQFGAGVLGFARITAKAAGYDGWMGVVITGLCIHLLIWMMYFLLKET 68 Query: 64 DGKPVFEIMERSIPKVILYPVYLGLCAVWLIFGCMIGKKYVLLFQMIAFPTTNPMXXXXX 123 +G + ++ ++ K I + A +LI + + YV + Q+ FPT + Sbjct: 69 NGNLI-DLQRQTFGKWIGNVFNIIFAAYFLIVSISVIRTYVEIIQVWMFPTASTFMLTLF 127 Query: 124 XXXXXXXXXIKGIFTISKAATIFFWLTFWMYFLLLWFFPSFRWVRLTPFILQGGHDQIKG 183 G I+ I T FL L+ W L P D +K Sbjct: 128 LCLVSYYIISSGFRVITGICVISVGGTLGYLFLSLFVLKYSHWDNLLPIFTHSFSDILKS 187 Query: 184 A-FDLYTVYLGYELSILLFPYMDKSKKSLAAIYAGNAYLSVIYISLSIVCFGFFSFGQXX 242 A +Y++ G+E+ ++++P++ K +S G + +++Y+ +++ F FFS Q Sbjct: 188 AQLSIYSM-TGFEIYLMVYPFVKKPMQSHKYAQLGALFSNLLYLFSTLLAFAFFSEKQLL 246 Query: 243 XXXXXXXXXXGYIQFPFVERLENMLYGFFLFLILITAAMYWWGAMEAMRRIIPKLKIPVM 302 IQ PF+ERLE + + +I+ + + W +M A I ++K + Sbjct: 247 KTIWSQLSMTQVIQLPFIERLEYIAISGYALVIISSFILPLWASMRATHEIF-RVKQRGV 305 Query: 303 VFLLTAIAYCISYIPKTLDQVNNWI 327 + I +S + +NN+I Sbjct: 306 LIAFIFITLIVSQLLTNRHDINNFI 330 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.334 0.148 0.484 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 456,412,655 Number of extensions: 16867094 Number of successful extensions: 84816 Number of sequences better than 1.0e-05: 22 Number of HSP's gapped: 84780 Number of HSP's successfully gapped: 22 Length of query: 359 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 232 Effective length of database: 412,420,005 Effective search space: 95681441160 Effective search space used: 95681441160 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 39 (21.6 bits) S2: 126 (53.1 bits)