BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0020.p (219 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_578083.1| hypothetical protein PF0354 [Pyrococcus furiosu... 129 3e-29 ref|NP_142471.1| hypothetical protein PH0499 [Pyrococcus horikos... 123 2e-27 ref|NP_127219.1| hypothetical protein [Pyrococcus abyssi] >gi|75... 120 1e-26 ref|NP_623508.1| conserved hypothetical protein [Thermoanaerobac... 115 9e-25 gb|EAE38373.1| unknown [environmental sequence] 77 2e-13 >ref|NP_578083.1| hypothetical protein PF0354 [Pyrococcus furiosus DSM 3638] gb|AAL80478.1| hypothetical protein [Pyrococcus furiosus DSM 3638] Length = 267 Score = 129 bits (325), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 74/222 (33%), Positives = 111/222 (50%), Gaps = 3/222 (1%) Query: 1 VGMGGTIAAFAAKGCEIHYLTVTNGDLGSSDGKNSPAEIAVIRSRELETAGRSLGATVFH 60 +GMGGTI + +G E+ Y+ +T+G +G++D K S E+A+IR RE E + + LG + Sbjct: 46 IGMGGTIKKLSDEGVEVIYICMTDGYMGTTDEKLSGHELALIRRREEEESAKLLGVRKIY 105 Query: 61 QLSHGDGTLXXXXXXXXXXXXXXRTVQPDAVFCPDPWLPYEAHYDHIVTGRAAAQAFLSS 120 L++ D L R +PD VF PDPWLPYE+H DH TG A ++ S Sbjct: 106 WLNYRDTELPYSREVRKDLVKIIRKEKPDGVFAPDPWLPYESHPDHRRTGFLAIESVAFS 165 Query: 121 GLALYPRGTSTRPWQPQALGF---YLTSEPNTVIDITSCFDQKLAAMALHSSQFSGETLA 177 L + +P ++ F Y T +PN ++DIT + KL A+ H SQF+ + Sbjct: 166 QLPNFSNIDIDIGLKPHSVSFIALYYTHKPNYIVDITDLMELKLKAIRAHRSQFTDDIWE 225 Query: 178 LYRLYFMEKGRQLAEGKDFEIGEGFKVLSPLHLHCFVDAGLV 219 + + E GEGF+V+ L H A L+ Sbjct: 226 TWEPFLRTVTMFYGEKIGVRYGEGFRVMPGLFYHITPFADLI 267 >ref|NP_142471.1| hypothetical protein PH0499 [Pyrococcus horikoshii] pir||F71162 hypothetical protein PH0499 - Pyrococcus horikoshii dbj|BAA29587.1| 272aa long hypothetical protein [Pyrococcus horikoshii] Length = 272 Score = 123 bits (309), Expect = 2e-27, Method: Compositional matrix adjust. Identities = 72/219 (32%), Positives = 107/219 (48%), Gaps = 10/219 (4%) Query: 1 VGMGGTIAAFAAKGCEIHYLTVTNGDLGSSDGKNSPAEIAVIRSRELETAGRSLGATVFH 60 +GMGGTI + G E+ Y+ +T+G +G++D S E+A IR +E E + R LG + Sbjct: 50 IGMGGTIKKLSDMGVEVIYVCMTDGYMGTTDESLSGHELAAIRRKEEEESARLLGVKKIY 109 Query: 61 QLSHGDGTLXXXXXXXXXXXXXXRTVQPDAVFCPDPWLPYEAHYDHIVTGRAAAQAFLSS 120 L++ D L R QPD VF PDPWLPYE+H DH TG A ++ S Sbjct: 110 WLNYRDTELPYSREVRKDLTKILRKEQPDGVFAPDPWLPYESHPDHRRTGFLAIESVAFS 169 Query: 121 GLALYPRGTST------RPWQPQA-LGFYLTSEPNTVIDITSCFDQKLAAMALHSSQFSG 173 L P ++T P+ + + Y T +PN ++DIT + KL A+ +H SQF Sbjct: 170 QL---PNFSNTDLDIGLNPYNSGSFIALYYTHKPNYIVDITDLMELKLKAIRVHRSQFPD 226 Query: 174 ETLALYRLYFMEKGRQLAEGKDFEIGEGFKVLSPLHLHC 212 + + + E GEGF+++ L H Sbjct: 227 DIWEKWEPFLRTIAMFYGEKIGVRYGEGFRIMPGLFYHI 265 >ref|NP_127219.1| hypothetical protein [Pyrococcus abyssi] pir||C75001 hypothetical protein PAB1341 - Pyrococcus abyssi (strain Orsay) emb|CAB50449.1| Hypothetical protein [Pyrococcus abyssi] Length = 267 Score = 120 bits (302), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 71/222 (31%), Positives = 103/222 (46%), Gaps = 3/222 (1%) Query: 1 VGMGGTIAAFAAKGCEIHYLTVTNGDLGSSDGKNSPAEIAVIRSRELETAGRSLGATVFH 60 +GMGGTI +G E+ Y +T+G +G+ D + E+A IR RE E + + LG + Sbjct: 46 IGMGGTIKKLTERGIEVIYACMTDGYMGTLDSSLTGHELATIRRREEEESSKLLGVKKIY 105 Query: 61 QLSHGDGTLXXXXXXXXXXXXXXRTVQPDAVFCPDPWLPYEAHYDHIVTGRAAAQAFLSS 120 L++ D L R +PD VF PDPWLPYEAH DH TG A A S Sbjct: 106 WLNYRDTELPYSREVRKDLVRIIRKEKPDGVFLPDPWLPYEAHPDHRNTGFLALDAVAFS 165 Query: 121 GLALYPR---GTSTRPWQPQALGFYLTSEPNTVIDITSCFDQKLAAMALHSSQFSGETLA 177 L + P Q + Y T++PN +DIT + KL A+ H SQF + Sbjct: 166 PLPNFSNVDVEIGLGPHQVSFIALYYTNKPNYFVDITDVMELKLKAIRTHKSQFPDDVWE 225 Query: 178 LYRLYFMEKGRQLAEGKDFEIGEGFKVLSPLHLHCFVDAGLV 219 ++ + + + EGF+++ L H A L+ Sbjct: 226 VWEPFLRTVALFYGKKAGVKYAEGFRIMPGLFYHITPFADLI 267 >ref|NP_623508.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM25112.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 260 Score = 115 bits (287), Expect = 9e-25, Method: Compositional matrix adjust. Identities = 78/231 (33%), Positives = 109/231 (47%), Gaps = 18/231 (7%) Query: 1 VGMGGTIAAFAAKGCEIHYLTVTNGDLGSSDGKNSPAEIAVIRSRELETAGRSLGATVFH 60 +G GG ++ KG +I ++TVT+G G + + EI IR E AG LG T F Sbjct: 30 IGAGGFLSILKEKGAKITFVTVTDGRKGLPTREITHDEIVKIRDAEKRKAGEILGVTEFI 89 Query: 61 QLSHGDGTLXXXXXXXXXXXXXXRTVQPDAVFCPDPWLPYEAHYDHIVTGRAAAQAFLSS 120 L DG RTV+P+ V D W PYEAH DH TG+ A L++ Sbjct: 90 DLGFEDGGNYTIDSLIEKLVRTFRTVKPELVLTVDGWAPYEAHPDHEKTGKGVTAAILAA 149 Query: 121 -GLALYPRGTSTRPWQPQALGFYLTSEPNTVIDITSCFDQKLAAMALHSSQFSGETLALY 179 L+L G P+ + FY T PNT IDIT+ ++ K+ A+ H+SQF Sbjct: 150 RNLSLPHLG---EPFSVPQIAFYATPYPNTYIDITNYWENKIEAIKAHTSQFDNPNWPFI 206 Query: 180 RLYFMEKGRQ-----LAEGK---------DFEIGEGFKVLSPLHLHCFVDA 216 +F E+ ++ L +GK + + E FKVLS LH F +A Sbjct: 207 YEFFYEQAKEWYKEGLVKGKLKSEKNTLNEEGLAEAFKVLSIWELHMFPNA 257 >gb|EAE38373.1| unknown [environmental sequence] Length = 223 Score = 77.4 bits (189), Expect = 2e-13, Method: Compositional matrix adjust. Identities = 54/191 (28%), Positives = 85/191 (44%), Gaps = 8/191 (4%) Query: 4 GGTIAAFAAKGCEIHYLTVTNGDLGSSDGKNSPAEIAVIRSRELETAGRSLGATVFHQLS 63 GGT+A ++KG +H + T+G+ G+ DG +P ++A +R +E A LGA + Sbjct: 24 GGTVALLSSKGWSVHIVVTTSGNKGTKDGSVTPQQLAFVREKEQRHAAEILGAEDPTFMG 83 Query: 64 HGDGTLXXXXXXXXXXXXXXRTVQPDAVFCPDPWLPYEAHYDHIVTGRAAAQAFL--SSG 121 DG + R +PD V D + H DH TGRA A + Sbjct: 84 FPDGYIENSNELRGLLVRQIRLHRPDLVITWDGFRSGFNHRDHRRTGRATYDAIYPAADD 143 Query: 122 LALYP--RGTSTRPWQPQALGFYLTSEPNTVIDITSCFDQKLAAMALHSSQFSGET---- 175 YP + + P +P L T P+ ++D+TS K+ ++ H SQ G T Sbjct: 144 HLFYPSHKRENLSPHRPSVLLLAGTDSPDYLVDVTSVMAIKVRSILAHESQMGGRTEAQV 203 Query: 176 LALYRLYFMEK 186 + YR +E+ Sbjct: 204 MERYRKRMIEE 214 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.416 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 309,839,185 Number of extensions: 11240474 Number of successful extensions: 20237 Number of sequences better than 1.0e-05: 27 Number of HSP's gapped: 20256 Number of HSP's successfully gapped: 27 Length of query: 219 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 98 Effective length of database: 428,710,599 Effective search space: 42013638702 Effective search space used: 42013638702 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)