BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0335.0009.p (416 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_616102.1| macrolide-efflux protein [Methanosarcina acetiv... 331 2e-89 ref|ZP_00077856.1| COG0477: Permeases of the major facilitator s... 327 4e-88 ref|NP_617177.1| hypothetical protein [Methanosarcina acetivoran... 309 7e-83 ref|ZP_00160481.1| hypothetical protein Avar272601 [Anabaena var... 179 1e-43 ref|ZP_00072931.1| COG0477: Permeases of the major facilitator s... 165 2e-39 >ref|NP_616102.1| macrolide-efflux protein [Methanosarcina acetivorans str. C2A] gb|AAM04582.1| macrolide-efflux protein [Methanosarcina acetivorans str. C2A] Length = 443 Score = 331 bits (849), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 166/367 (45%), Positives = 242/367 (65%), Gaps = 3/367 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IGSGLT F+LG+YV+ TGTA+S + +CA++P +L P+GG+LADR DRRLM Sbjct: 18 GQFISIIGSGLTTFSLGIYVYQQTGTASSYVFILMCAFLPPFLLKPYGGILADRHDRRLM 77 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 MI GDLGS +GL+FI +ML+G L QI LG+A+SSVF + EPAYKA ITDLL Q+ Sbjct: 78 MILGDLGSTLGLLFIFAMMLKGNIELWQIYLGIAISSVFSAFQEPAYKALITDLLPENQY 137 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 AKASGL+QLA+S++YL+SP LAG +L ++ ++ + Sbjct: 138 AKASGLMQLASSAQYLISPFLAGIILTIMDIKFVFLIDVTTFLIASSIVIWIRKILSRTQ 197 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 +T DL+EG + + +G++ L+ +++ F G LQ+LF P++L T + Sbjct: 198 ---ITKQEQNNMADLKEGIQEFSKNRGIVNLVITTTLILFFVGLLQSLFIPMMLNLTTVK 254 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 +G I+SI A G+L GSL IG++ K V+ L++SL V+G+ FA +G+STN IT AG Sbjct: 255 AVGTIQSICASGILVGSLFIGVYGSKNKYVKTLSISLFVSGIFFANLGLSTNVIFITLAG 314 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FF+ LPF+NT+ +V+IR NI QGR W II +++ G + A+A +G L+D++FNPL Sbjct: 315 FMFFSTLPFINTAIEVLIRKNIENSKQGRIWSIISMVTYLGSIIAFAVAGFLADKIFNPL 374 Query: 362 LQEGGML 368 L+ G+L Sbjct: 375 LESDGLL 381 >ref|ZP_00077856.1| COG0477: Permeases of the major facilitator superfamily [Methanosarcina barkeri] Length = 448 Score = 327 bits (838), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 166/370 (44%), Positives = 239/370 (64%), Gaps = 3/370 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IG+GLT F+LGVYV+ T TA+S + +C ++P +L P+GG+LADR+DRRLM Sbjct: 25 GQFISVIGTGLTVFSLGVYVYQQTNTASSYVFILMCVFLPPFLLKPYGGILADRYDRRLM 84 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 MI GDLG+ +GL+FI +ML+G L QI LG+A SSVF + EPAYKA ITDLL Q+ Sbjct: 85 MILGDLGATLGLLFIFFMMLKGNIQLWQIYLGIATSSVFSAFQEPAYKALITDLLPEAQY 144 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 A+ASGLVQLA+S++YL+SP LAG LL + N Sbjct: 145 ARASGLVQLASSAQYLISPFLAGILLTIIDIKFIFLIDTTTLLISSSIVIWIR---NISG 201 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 + + DL+EG K ++ +GV+ L+ + + F G LQ+LF PLLL+ T + Sbjct: 202 KVKIDQHKQNIIADLKEGMKEFSNNRGVVNLVIITMITLFFVGLLQSLFIPLLLSLTTVK 261 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 T G+ +SI A G+L GSL IG+F K V+ L++SL ++G+ FA +G+S N +T AG Sbjct: 262 TAGISQSICASGILIGSLFIGVFGSKNRYVKTLSISLFLSGIFFANLGISKNILFVTLAG 321 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FFA LPF+NTS +V+IR NI + QGR W +I +++ G + A+A +G L+D++FNPL Sbjct: 322 FMFFATLPFINTSIEVLIRKNIDNKKQGRVWSLISMITYLGSIMAFAVAGFLADKIFNPL 381 Query: 362 LQEGGMLASS 371 L+ G++ + Sbjct: 382 LEVDGLMVET 391 >ref|NP_617177.1| hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM05657.1| hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 456 Score = 309 bits (792), Expect = 7e-83, Method: Compositional matrix adjust. Identities = 161/370 (43%), Positives = 229/370 (61%), Gaps = 3/370 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G++IS IG+ LT F+LG+YV+ TG A+S + +C ++P +L P+GG+LADR+DRRLM Sbjct: 32 GQFISIIGTDLTIFSLGIYVYQQTGAASSYVFILMCVFLPPFLLKPYGGILADRYDRRLM 91 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 M+ GDLG+ +GL+FI +M+ G L I LG+A SS+F ++ EPAYKA ITDLL Q+ Sbjct: 92 MVLGDLGATLGLLFIFFMMIRGNIELWHIYLGIAASSIFSAIQEPAYKALITDLLPEAQY 151 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 KASGLVQLA+S++YL+SP LAG LL ++ + Sbjct: 152 DKASGLVQLASSARYLISPFLAGILLTLIDIEFTFLIDISPLLISSSIVIWVRKFLG--- 208 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 + + D +E + + GV+ L+ +V F G LQ+LF P+LL T + Sbjct: 209 KTTIKHPEQNIIADFKEDIEEFSKNGGVVNLVLTTMLVLFFVGLLQSLFIPMLLGLTTVK 268 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 T+G+ +SI A G+L GSL +G+F K V+ LA SL +AG+ FA G+STN IT AG Sbjct: 269 TVGISQSICASGILIGSLFLGVFGCKSKQVKTLAFSLFLAGIFFANPGLSTNIIFITLAG 328 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 F+FF LPF+NTS +V+IR NI QGR W II +++ G +AA+A +G L+D+VFNP Sbjct: 329 FMFFGTLPFINTSIEVLIRKNIENRKQGRVWSIISMVTYLGSIAAFAVAGFLADKVFNPP 388 Query: 362 LQEGGMLASS 371 L+ G L + Sbjct: 389 LELDGFLVET 398 >ref|ZP_00160481.1| hypothetical protein Avar272601 [Anabaena variabilis ATCC 29413] Length = 443 Score = 179 bits (454), Expect = 1e-43, Method: Compositional matrix adjust. Identities = 112/371 (30%), Positives = 185/371 (49%), Gaps = 2/371 (0%) Query: 2 GEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRLM 61 G+ IS IGS LT FALGV+V+ TG+ T A+++L +P I++ P G L D+F RR M Sbjct: 24 GQMISLIGSQLTNFALGVWVYQRTGSVTQFALISLFTSLPMILISPVAGTLVDQFPRRWM 83 Query: 62 MITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQF 121 M+ DLG+ + I +L+ G+ + I +G A+SS F + PAY A T L+ E+ Sbjct: 84 MLFSDLGAGISTGVIAILLATGDLATWHIYVGAAISSCFGAFQWPAYTAATTLLVPPEKL 143 Query: 122 AKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTPK 181 A+A+G++Q+ ++ L++P+L G LL Sbjct: 144 ARANGMLQVGEAAGRLVAPMLGGILLLFLEIDGIIFIDFATFLFALSTLLLAPFPKQYID 203 Query: 182 SAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDTR 241 P K+ G L + +G+ L+ ++ F G +Q L TPL+L+F Sbjct: 204 RHRAEKTP--WLKEASSGLVYLVNRRGLFALLLFFAVNNFLVGIVQMLITPLVLSFGSAT 261 Query: 242 TLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSAG 301 LG I + +GML S+++ + + + + + G+C G + + Sbjct: 262 DLGTIMTTGGIGMLVSSILVSTVRMPQYLALSIFTFMLLGGICITCAGFYQSILALALIA 321 Query: 302 FLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNPL 361 FLFF LP +N+SA V+ + +P +QGR + IG ++ AY +G L+D++F PL Sbjct: 322 FLFFFGLPIINSSAQVIFQKKVPSSLQGRVFATIGAIANASQPLAYTVAGPLADKIFEPL 381 Query: 362 LQEGGMLASSV 372 + + G+LA S+ Sbjct: 382 MAQNGLLAESM 392 >ref|ZP_00072931.1| COG0477: Permeases of the major facilitator superfamily [Trichodesmium erythraeum IMS101] Length = 445 Score = 165 bits (417), Expect = 2e-39, Method: Compositional matrix adjust. Identities = 103/372 (27%), Positives = 187/372 (50%) Query: 1 MGEWISSIGSGLTAFALGVYVFHMTGTATSVAMVTLCAYVPSIVLGPFGGVLADRFDRRL 60 +G+ S +GS L+ FAL V+V+ TG+ T A+++L ++PSI + PF G + DR+ RR Sbjct: 9 LGQVFSLLGSSLSEFALAVWVYQTTGSITHYALLSLFIHLPSIFISPFAGAMVDRWSRRQ 68 Query: 61 MMITGDLGSAVGLVFILVLMLEGEPSLGQICLGVALSSVFVSLLEPAYKATITDLLSVEQ 120 MI D + V + I+ L+ + + I + V +SSV + PAY + I L+ + Sbjct: 69 AMIVSDSITGVTALAIMFLVFSQQLQVWHIYIAVGISSVCNAFQWPAYISVIPQLVPPKH 128 Query: 121 FAKASGLVQLAASSKYLLSPVLAGSLLAXXXXXXXXXXXXXXXXXXXXXXXXXKRSMNTP 180 ++A+G+VQ A ++ +L P +AG L+ K ++ Sbjct: 129 LSRANGMVQGAIATARILGPSIAGVLIIKIHLHGILLIDFSTYLLAFFTLLSVKFPISQQ 188 Query: 181 KSAAVTGVPLQLFKDLREGWKALASVQGVLQLIFVLSMVTFCAGFLQTLFTPLLLAFTDT 240 + +L++++ GW + G+ QL ++ AG LQ LF P++L+F Sbjct: 189 QKIRRKVSIEKLWQEVISGWNYVVVRTGLRQLTIFFGVIYLSAGILQVLFLPMVLSFASE 248 Query: 241 RTLGMIESISAVGMLAGSLVIGIFSLKGNIVRLLAVSLGVAGLCFALVGMSTNTALITSA 300 + LG++ S+S +GML GS+++ ++ + + V + + GLC L G+ L Sbjct: 249 KELGIVMSVSGIGMLLGSVIVSLWGCSKRRIEGILVFVFIQGLCLCLGGLKPAVILAAIG 308 Query: 301 GFLFFAALPFVNTSADVMIRSNIPGEVQGRAWGIIGVLSQFGYVAAYACSGVLSDQVFNP 360 GF + + V + + + +P +QGR + + ++ +F V + + L + +F P Sbjct: 309 GFGYLCSRAIVVSLNHTIWQQKVPQYLQGRVFSLQNMMEKFLLVITHLSAAPLVEFMFKP 368 Query: 361 LLQEGGMLASSV 372 L+ E G+LASSV Sbjct: 369 LMSENGILASSV 380 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.325 0.138 0.394 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 474,353,959 Number of extensions: 16816255 Number of successful extensions: 67332 Number of sequences better than 1.0e-05: 171 Number of HSP's gapped: 67410 Number of HSP's successfully gapped: 181 Length of query: 416 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 288 Effective length of database: 409,704,906 Effective search space: 117995012928 Effective search space used: 117995012928 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 127 (53.5 bits)