BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0334.0005.p (476 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_827549.1| putative secreted sugar-binding protein [Strept... 297 4e-79 ref|NP_626149.1| putative secreted sugar-binding protein [Strept... 280 6e-74 ref|NP_102567.1| probable secreted solute-binding protein [Mesor... 145 3e-33 ref|NP_386617.1| PUTATIVE SUGAR-BINDING PERIPLASMIC ABC TRANSPOR... 144 5e-33 ref|NP_357427.1| AGR_L_3270p [Agrobacterium tumefaciens] >gi|179... 139 1e-31 >ref|NP_827549.1| putative secreted sugar-binding protein [Streptomyces avermitilis MA-4680] dbj|BAC74084.1| putative secreted sugar-binding protein [Streptomyces avermitilis MA-4680] Length = 445 Score = 297 bits (760), Expect = 4e-79, Method: Compositional matrix adjust. Identities = 156/440 (35%), Positives = 243/440 (55%), Gaps = 6/440 (1%) Query: 25 LKESFMKKSQRAACIFLIVVLGLLLSGCFPKSDNAA--DTAEHGGKVVLTYWDENAGPSR 82 +K S + + A + L VL L + C AA E GK +T+WD N G R Sbjct: 1 MKISIHRSRRAATAVALGSVLALTATACGDDGSGAAGDKGTEGSGKGEITFWDNNGG-VR 59 Query: 83 TPILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAK 142 T I ++++ FE+K PDI+V Y G+PA ++KYD A+ G LPD GG+ +A+ A + Sbjct: 60 TDIWKQIIADFEKKYPDIKVNYVGIPAASAQSKYDTAIQGGGLPDVGGVGTAMLAEVAVQ 119 Query: 143 GVLLPLDAYFADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGL 202 G L PLD+ G++ + +D +R+ LYQ+P++ WYR+D F +AGL Sbjct: 120 GALEPLDSRLKKSSLNGELSQNLLDSSRAAGGGDTLYQVPTSANNGTLWYRTDLFKSAGL 179 Query: 203 PRPQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTV 262 P TW + + L + + FG+++RGGEGS+A +Y +GIT F +TV Sbjct: 180 DAPTTWSTFYEAADKLTDKGKNKFGFTIRGGEGSIAPALDAVYGQTGIT-EFWNGEKTTV 238 Query: 263 NDPVHIEFLKRYLALYKAYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKKALGT 322 NDP ++ L++Y+ALYK TP++D+ + + ++VA +DSG M+ HNLGSYQDH KALG Sbjct: 239 NDPKNVAALEKYIALYKKDTPSADVNNDFTKMVAQWDSGTIGMLSHNLGSYQDHLKALGA 298 Query: 323 GHFDGVLLPPSINGTRTLFGN-ANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQ 381 G F G+ P +GTR N +G + +++++ AW+ I + +S +N GQ Sbjct: 299 GKFKGIPNPTRDDGTRVQISNPVDGMSVFKSSKNKAAAWKFIEFAVSSAENSKFNESAGQ 358 Query: 382 IPTNLKAWNDPYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLIRLI-EPEFQQVLA 440 +P N +A D + + A EA DT V+ PY LPD+ ++ + EP FQ++L Sbjct: 359 VPANTEAAKDTWVQQAEPTKLAAEALNGADTKIVQLPYYLPDWNTISKADNEPNFQKLLL 418 Query: 441 GTMDVEHFLNFWAQLMENSR 460 G M + FL+ A + ++ Sbjct: 419 GKMSAKEFLDTLADQLNKAQ 438 >ref|NP_626149.1| putative secreted sugar-binding protein [Streptomyces coelicolor A3(2)] emb|CAC38817.1| putative secreted sugar-binding protein [Streptomyces coelicolor A3(2)] Length = 452 Score = 280 bits (716), Expect = 6e-74, Method: Compositional matrix adjust. Identities = 148/404 (36%), Positives = 231/404 (57%), Gaps = 7/404 (1%) Query: 64 EHGGKVVLTYWDENAGPSRTPILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASG 123 E GK +T+WD N G RT + + ++ FE+ NPDI V+Y G+ A ++K D A+ G Sbjct: 45 EGSGKGKITFWDNNGGI-RTAVWKTIIADFEKANPDIDVEYVGIAATEYQSKVDTAIQGG 103 Query: 124 DLPDAGGINGEWIADYAAKGVLLPLDAYFADWPERGQIDPSFIDYNRSLTQ-DGKLYQIP 182 LPD GG+ +A ++A+G L PLD P +++ ++ ++ DG LY +P Sbjct: 104 GLPDVGGVTASMLAGFSAQGALEPLDDRLGKSPLSDKVNKDMVESLKAAGGGDGTLYSMP 163 Query: 183 STYYLDVFWYRSDWFAAAGLPRPQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTS 242 ++ V +YR+D F AGL P TW+ + + L + + FGY++RGG GS+AQ Sbjct: 164 TSANNGVLYYRTDLFEKAGLDAPTTWDKFYAAAEKLTDAGKNEFGYTIRGGSGSIAQALD 223 Query: 243 LMYAYSGITGSFRTDGS--STVNDPVHIEFLKRYLALYKAYTPASDIVSGYKEVVANFDS 300 MY SGIT SF G +TVNDP ++E L++Y ALYK TPA+D+ + + ++VA +D+ Sbjct: 224 AMYGQSGIT-SFWDAGKKKTTVNDPKNVEALEKYAALYKKVTPAADVNNDFAKMVAQWDT 282 Query: 301 GRAAMIQHNLGSYQDHKKALGTGHFDGVLLPPSINGTRTLFGN-ANGYGIMRTTRHPDEA 359 G M+ HNLGSYQDH KALG F G+ P +G R N +G G+ +++++ + A Sbjct: 283 GTIGMLNHNLGSYQDHVKALGVDKFRGIPQPVGASGKRVQVSNPVDGLGMFKSSKNKEAA 342 Query: 360 WRLIRYLLSEESQIYWNSQVGQIPTNLKAWNDPYFLSHPVLREAIEAFRSPDTAFVRAPY 419 W+ I + S+E +N GQ+P++ +A ND + + A +A T V+ PY Sbjct: 343 WKFIEFATSKEQNSKFNESAGQVPSSTEAANDAWIQKAEPTKLAAQALNDGSTTIVQLPY 402 Query: 420 ELPDYPSLIRL-IEPEFQQVLAGTMDVEHFLNFWAQLMENSRRQ 462 LPD+ +L + EP FQ+VL G M + FL+ A + ++ + Sbjct: 403 YLPDWNTLSKSESEPGFQKVLLGDMSAKDFLDGLADKLNEAQAE 446 >ref|NP_102567.1| probable secreted solute-binding protein [Mesorhizobium loti MAFF303099] dbj|BAB48353.1| probable secreted solute-binding protein [Mesorhizobium loti] Length = 430 Score = 145 bits (365), Expect = 3e-33, Method: Compositional matrix adjust. Identities = 110/438 (25%), Positives = 183/438 (41%), Gaps = 15/438 (3%) Query: 33 SQRAACIFLIVVLGLLLSGCFPKSDNAADTAEHGGKVVLTYWDENAGPSRTPILQELLRR 92 + + + L V GL +S +D+ E V+T P RT L++ + Sbjct: 5 NMKKLTVMLATVAGLAISAGSALADSTLKMVE-----VIT------SPPRTEFLKKQIAE 53 Query: 93 FEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADYAAKGVLLPLDAYF 152 FE NP ++V+ +P G K+ V +GD PD + W+ YA L L Y Sbjct: 54 FEAANPGVKVELISLPWGQAFEKFLTMVQAGDTPDVVEMPERWMGLYANNAQLEDLGPYM 113 Query: 153 ADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAAAGLPRPQTWEDIF 212 A W + + + ++ + + IP YY++ ++ F AGL P D F Sbjct: 114 AKWDDAKTLGERAKQFGSTV--NNTQFMIPYGYYVNALFWNKKLFKQAGLDGPPATLDEF 171 Query: 213 RSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGSSTVNDPVHIEFLK 272 + + G +GY LRGG G+ + M G G F DG+ST+N+ ++ L+ Sbjct: 172 VADSKKISAIPGKYGYCLRGGPGAFNGMHMFMNIAQGKGGYFNKDGTSTINEEGSVKGLQ 231 Query: 273 RYLALYKAYTPASDIVS-GYKEVVANFDSGRAAMIQHNLGSYQDHKKALGTGHFDGVLLP 331 +YK D VS G+ E V F SG AM+ + + + F LP Sbjct: 232 MLADMYKDGLAPKDAVSWGFNETVTGFYSGTCAMLNQDPDALLGIADKMSADDFAVAPLP 291 Query: 332 PSINGTRTLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQVGQIPTNLKAWND 391 +G G+ + ++H D+AW+L+ LLS + + W +VG +P + A D Sbjct: 292 VGPSGKSYPTLGYAGWAMFANSQHKDDAWKLMATLLSPKDNLEWAKEVGVVPIHNGADQD 351 Query: 392 PYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLIRLIE-PEFQQVLAGTMDVEHFLN 450 P+F + E + V P L + + + + FQ+VL G + + Sbjct: 352 PHFKTEQFKGWFTELSDTSKYEMVTPPTHLENLGNFVDQVAIKNFQEVLLGQKTAKDVAD 411 Query: 451 FWAQLMENSRRQHLHSNE 468 WA + ++ L N+ Sbjct: 412 QWAAFLTKEQQDWLAKNK 429 >ref|NP_386617.1| PUTATIVE SUGAR-BINDING PERIPLASMIC ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] emb|CAC47090.1| PUTATIVE SUGAR-BINDING PERIPLASMIC ABC TRANSPORTER PROTEIN [Sinorhizobium meliloti] Length = 420 Score = 144 bits (363), Expect = 5e-33, Method: Compositional matrix adjust. Identities = 102/392 (26%), Positives = 169/392 (43%), Gaps = 7/392 (1%) Query: 80 PSRTPILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADY 139 P RT L+ ++ RFEQ NP V+ +P G K+ V++G++PD + W++ Y Sbjct: 33 PERTETLKSIVARFEQANPGTTVEIISLPWGEAFQKFATMVSAGEIPDVMEMPDTWLSLY 92 Query: 140 AAKGVLLPLDAYFADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAA 199 A G+L L+ Y W + ++ R + Y +P +YL +Y + Sbjct: 93 ANNGMLESLEPYLEKWEHTPGLTERALELGRDVNDTA--YMLPYGFYLRAMFYNKKLLSE 150 Query: 200 AGLPRPQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGS 259 AG+ P D F + G GY LRGG G + +G F DG+ Sbjct: 151 AGVAEPPKTMDDFVKASEAVSKLPGKSGYCLRGGPGGLNGWVMFGATMAGDNKFFNEDGT 210 Query: 260 STVNDPVHIEFLKRYLALYK-AYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKK 318 ST+N I+ L + LYK P + G+ E+VA F SG A + + + + Sbjct: 211 STMNSEGWIKGLTWVIDLYKNGLAPKDSVNWGFNEIVAGFYSGTCAFLDQDPDALIAIAQ 270 Query: 319 ALGTGHFDGVLLPPSINGTR-TLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNS 377 + F +P +G T G A G+ I+ +++ D +W+LI L E I WN Sbjct: 271 RMKPEDFGVTTMPKGPSGKAFTTIGFA-GWSILAASQNKDLSWKLIETLEGPEGNIEWNK 329 Query: 378 QVGQIPTNLKAWNDPYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLI-RLIEPEFQ 436 + G +P + A DP++ S P + + D P L ++ L Q Sbjct: 330 RTGALPVHKSAEKDPFYAS-PQFKGWFDELADKDVVLTVMPTYLEEFAFFKDSLAIKTTQ 388 Query: 437 QVLAGTMDVEHFLNFWAQLMENSRRQHLHSNE 468 + L G + E N WA + +++++L + + Sbjct: 389 EALLGDITPEELANQWADYLTKAQQKYLANKK 420 >ref|NP_357427.1| AGR_L_3270p [Agrobacterium tumefaciens] ref|NP_533674.1| ABC transporter, substrate binding protein [sugar] [Agrobacterium tumefaciens str. C58 (U. Washington)] pir||B98336 srrA protein [imported] - Agrobacterium tumefaciens (strain C58, Cereon) pir||AH2946 hypothetical protein Atu3174 [imported] - Agrobacterium tumefaciens (strain C58, Dupont) gb|AAK90212.1| AGR_L_3270p [Agrobacterium tumefaciens str. C58 (Cereon)] gb|AAL43990.1| ABC transporter, substrate binding protein [sugar] [Agrobacterium tumefaciens str. C58 (U. Washington)] Length = 419 Score = 139 bits (351), Expect = 1e-31, Method: Compositional matrix adjust. Identities = 96/390 (24%), Positives = 166/390 (42%), Gaps = 5/390 (1%) Query: 80 PSRTPILQELLRRFEQKNPDIQVKYEGMPAGINKAKYDLAVASGDLPDAGGINGEWIADY 139 P RT L+ ++ +FE NP +V+ +P K+ V++GD+PD + W++ Y Sbjct: 33 PERTETLKGIVSKFEAANPGTKVEIISLPWSEAFQKFATMVSAGDVPDVMEMPDTWLSLY 92 Query: 140 AAKGVLLPLDAYFADWPERGQIDPSFIDYNRSLTQDGKLYQIPSTYYLDVFWYRSDWFAA 199 A G+L L+ Y A W + ++ R + + Y +P +YL +Y A Sbjct: 93 ANNGMLESLEPYLAKWEYTKGLSERTLELGRDVKETA--YMLPYGFYLRAMFYNKKLLAE 150 Query: 200 AGLPRPQTWEDIFRSVRVLANTRQGSFGYSLRGGEGSVAQLTSLMYAYSGITGSFRTDGS 259 AG+ P + F G +GY LRGG G + + +G F DG+ Sbjct: 151 AGVKEPPKTLEEFADASKKVAALPGKYGYCLRGGPGGLNGWVMFGASMAGSNEFFTKDGT 210 Query: 260 STVNDPVHIEFLKRYLALYK-AYTPASDIVSGYKEVVANFDSGRAAMIQHNLGSYQDHKK 318 ST + P ++ L + LYK P + G+ E+VA F SG A + + + + Sbjct: 211 STFDSPGWVKGLTYVIDLYKNGLAPKDSVNWGFNEIVAGFYSGTCAFLDQDPDALIAIAQ 270 Query: 319 ALGTGHFDGVLLPPSINGTRTLFGNANGYGIMRTTRHPDEAWRLIRYLLSEESQIYWNSQ 378 + F + +P +G G+ IM + + D +W+L+ L E I WN + Sbjct: 271 RMKPEDFGVMSMPKGPDGKTFPTIGFAGWSIMSASANKDLSWKLVETLEGPEGNIEWNKK 330 Query: 379 VGQIPTNLKAWNDPYFLSHPVLREAIEAFRSPDTAFVRAPYELPDYPSLI-RLIEPEFQQ 437 G +P + A DP++ S + + + + P L ++ L+ Q+ Sbjct: 331 TGALPVHKSAEKDPFYASEQ-FKGWFDELADKNAVPTKMPTYLEEFAFFKDSLVIKTSQE 389 Query: 438 VLAGTMDVEHFLNFWAQLMENSRRQHLHSN 467 L G + E WA M ++++ L S Sbjct: 390 ALLGDITPEDLAKQWADYMTKAQQKFLASK 419 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.137 0.422 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 750,918,981 Number of extensions: 33135838 Number of successful extensions: 71918 Number of sequences better than 1.0e-05: 171 Number of HSP's gapped: 71905 Number of HSP's successfully gapped: 171 Length of query: 476 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 347 Effective length of database: 406,989,807 Effective search space: 141225463029 Effective search space used: 141225463029 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 128 (53.9 bits)