BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0010.p (393 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_624025.1| Permeases of the major facilitator superfamily ... 135 2e-30 ref|NP_391713.1| alternate gene name: ipa-21r~similar to sugar p... 124 3e-27 ref|NP_691645.1| hypothetical protein OB0724 [Oceanobacillus ihe... 108 2e-22 ref|ZP_00061501.1| COG0477: Permeases of the major facilitator s... 98 4e-19 ref|NP_243142.1| BH2276~unknown conserved protein [Bacillus halo... 96 1e-18 >ref|NP_624025.1| Permeases of the major facilitator superfamily [Thermoanaerobacter tengcongensis] gb|AAM25629.1| Permeases of the major facilitator superfamily [Thermoanaerobacter tengcongensis] Length = 382 Score = 135 bits (339), Expect = 2e-30, Method: Compositional matrix adjust. Identities = 90/300 (30%), Positives = 153/300 (51%), Gaps = 5/300 (1%) Query: 33 GLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVL-ILSVAVPAVLAVFYQSEYFI 91 G IG+LM+ G +AI QP WG+ SDR VL IL +A ++A+F S F Sbjct: 37 GYSKSEIGILMSLGPFVAIIAQPFWGITSDRARSKNFVLQILFLASIFIMALFPLSRNFY 96 Query: 92 VLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQYVSHF 151 L+ V TL T F ++ PI+D+ + + G ++G IRM +IG AV + + Sbjct: 97 YLVAVITLFTFFQTSTGPISDAITLEYLSGTGQSFGPIRMAGTIGYAVMSVIAGWFAQR- 155 Query: 152 SVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFLGGSFLV 211 + +I+ LP + ++ + S I L N+ + + +FL+ Sbjct: 156 HIGSIFILNIIVMFIAFMIVYKLP-VVKGHQFGKEKIS--IWKLFSNRNLVVLMLLNFLI 212 Query: 212 NQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTLLLGA 271 T+ + TYF + F+ +GGS G+A FI++ + P +LYA K++K++G L+ + Sbjct: 213 QTTLGFYYTYFPIYFRKIGGSNELLGLAYFISAMSEIPFLLYADKILKRIGTRWALIGAS 272 Query: 272 LIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSVFNIV 331 + LRW I P +++ Q+LHGL F + Y + Y++ E++A+GQ+V N++ Sbjct: 273 GVAALRWLIISFIPNAYLVLPFQLLHGLIFIVLYYSMATYINQEVPKELKASGQTVNNLI 332 >ref|NP_391713.1| alternate gene name: ipa-21r~similar to sugar permease [Bacillus subtilis] sp|P39589|YWBF_BACSU Hypothetical transport protein ywbF pir||S39676 sugar permease homolog ywbF - Bacillus subtilis emb|CAA51577.1| ipa-21r [Bacillus subtilis] emb|CAB15860.1| ywbF [Bacillus subtilis subsp. subtilis str. 168] Length = 399 Score = 124 bits (312), Expect = 3e-27, Method: Compositional matrix adjust. Identities = 81/311 (26%), Positives = 149/311 (47%), Gaps = 5/311 (1%) Query: 28 LLKQGGLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILSVAVPAVLAVFYQS 87 L K+ L ++G++M+ G ++ I QP WG++SD + + +L + ++ ++ + Y + Sbjct: 35 LTKEQHLSGSQVGLIMSLGPIVMIFFQPFWGMLSDYTQKTKGLLAVCTSITGIIGLAYIA 94 Query: 88 -EYFIVLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQ 146 + F + +++ F ST P++DS ++ + YG IR+ S+G V +A Q Sbjct: 95 FDSFPLFILIAACFAAFQSTIIPLSDSISLRYTQETNGNYGGIRLFGSLGFGVAVFAMGQ 154 Query: 147 YVSH-FSVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFL 205 + + + I+ +P Q + ++ + KG + L+ NK FL F+ Sbjct: 155 VTNQLYPIHVIFIFGCAFLCIAAILASQVPGQQKTTKV---NIRKGFRELISNKTFLIFM 211 Query: 206 GGSFLVNQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRER 265 +F A TYF L G S S GI FI + P M +A I K+G Sbjct: 212 IITFTTFAPNLANNTYFSLFLDKSGASLSAIGILFFIGVISEIPFMRFAQTFIDKMGLLN 271 Query: 266 TLLLGALIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQ 325 ++L + + RW + F P+ ++ L G++ GLF AA++YV IT ++AT Sbjct: 272 VIMLSGGVSLFRWALYFTAPSLWIIYATVFLQGVAIGLFIPAALQYVKKITPRHVEATAL 331 Query: 326 SVFNIVFSGFA 336 +++ + +GF Sbjct: 332 TMYAAIGNGFG 342 >ref|NP_691645.1| hypothetical protein OB0724 [Oceanobacillus iheyensis HTE831] dbj|BAC12680.1| hypothetical conserved protein [Oceanobacillus iheyensis HTE831] Length = 390 Score = 108 bits (271), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 87/310 (28%), Positives = 145/310 (46%), Gaps = 6/310 (1%) Query: 27 LLLKQGGLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILSVAVPAVLA-VFY 85 L L+ GL +IG ++A +L AI QP WG +SD++ + +L + + + F+ Sbjct: 30 LYLQSRGLSGTQIGWVLAVESLAAILAQPFWGFLSDKFKTIKRILFICLIGLLIFGFTFF 89 Query: 86 QSEYFIVLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGS 145 Q +L+ + F++ + DS+A A + G ++GSIRM S+G A+ + Sbjct: 90 QMSSLHTILIFGAVFYFFAAPIGGLNDSFAQRRAGQLGISFGSIRMWGSVGFALSSLLVG 149 Query: 146 QYVSHFSVS-TIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAF 204 + ++ F + +W +A+ + SK IK +K FL F Sbjct: 150 EVLARFGIGYMVWVYLAFGTIALLTLIPLKDVKADTKPVKLNDISKIIK----SKPFLLF 205 Query: 205 LGGSFLVNQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRE 264 LG + T Y L +GGS G+A F + A+ +K Sbjct: 206 LGVILFITITHRMNDYYMALYISELGGSEDLVGLAWFAGVISEAAVFALAAFWFRKFHSL 265 Query: 265 RTLLLGALIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATG 324 +++ A+IY LRW + P ++IG+Q LHGL+FG+FY AA +YV+ + +Q+TG Sbjct: 266 IFIIIAAIIYTLRWFLYATATDPMMIIGLQFLHGLTFGVFYTAAFDYVTRLIPSFLQSTG 325 Query: 325 QSVFNIVFSG 334 VF V+ G Sbjct: 326 HLVFFSVYFG 335 >ref|ZP_00061501.1| COG0477: Permeases of the major facilitator superfamily [Clostridium thermocellum ATCC 27405] Length = 391 Score = 97.8 bits (242), Expect = 4e-19, Method: Compositional matrix adjust. Identities = 71/304 (23%), Positives = 149/304 (49%), Gaps = 13/304 (4%) Query: 33 GLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILSVAVPAV-LAVFYQSEYFI 91 G D+ IG ++ + + + QP+WGVISDR VL + V ++ + +F+ S + Sbjct: 42 GYDNTDIGTFLSISSFVGLFAQPIWGVISDRAKSKNNVLKMLVLFSSIAIFMFHLSGNYY 101 Query: 92 VLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQYVSHF 151 + V + F + PI D+ + YG IR+ ++G AV A+ G ++ Sbjct: 102 YIFAVMVVYAFFQTPITPIGDAITLEYITDTKWKYGPIRLAGALGYAVMAFIGGA-LTRK 160 Query: 152 SVSTIWXXXXXXXXXXXXXXXTLP----KQAEENRMMSQSFSKGIKTLLGNKIFLAFLGG 207 +++ I+ +P Q++ N++ I + N + +G Sbjct: 161 NINAIFFICFVIGIMSLITVFRMPTVKGHQSDGNKL-------SILEVFKNSELVLLMGF 213 Query: 208 SFLVNQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTL 267 + +++ TM + T+F + ++++G + G+A+FI S + +++ ++IK+LG + TL Sbjct: 214 TLVIHTTMGFYNTFFPIYYKNMGADNTILGLAVFIGSASEIIFLVFGDRIIKRLGIKFTL 273 Query: 268 LLGALIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSV 327 A++ V+RW ++ +Q+LHG F + + Y+++ PE++A+GQ+V Sbjct: 274 FGAAVVAVVRWASLGLINNIFAVLALQILHGFIFIVLAYSMATYINNEMPPELKASGQTV 333 Query: 328 FNIV 331 +++ Sbjct: 334 NSVI 337 >ref|NP_243142.1| BH2276~unknown conserved protein [Bacillus halodurans] pir||D83934 hypothetical protein BH2276 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05995.1| BH2276~unknown conserved protein [Bacillus halodurans] Length = 392 Score = 96.3 bits (238), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 81/301 (26%), Positives = 136/301 (45%), Gaps = 12/301 (3%) Query: 33 GLDSGRIGMLMATGTLIAICIQPLWGVISDRYNQARLVLILS-----VAVPAVLAVFYQS 87 GL +G IG+LMA G I QP WG +SD+Y + +L ++ VA+ A + + Sbjct: 36 GLTAGEIGVLMAVGPFAMILAQPTWGFLSDKYKSIKRMLQIALIGMIVAIFAFMGAASFA 95 Query: 88 EYFIVLLVVYTLSTIFSSTQAPIADSYAIVAANKAGSTYGSIRMMLSIGAAVGAYAGSQY 147 YFI++ V++ +F S + DS A A ++G IRM S+G + A Sbjct: 96 GYFIMMFVLF----LFLSPTTALGDSLAQKTAINRRLSFGKIRMWGSLGFGFTSLAVGYI 151 Query: 148 VSHFSVSTIWXXXXXXXXXXXXXXXTLPKQAEENRMMSQSFSKGIKTLLGNKIFLAFLGG 207 ++ V+ I L ++ + + +K + KIF FL Sbjct: 152 LAAIGVTYIMVPLLVVTVISLWLSFGLEDFSDTTK--PVTLLSALKLAIDPKIFF-FLVC 208 Query: 208 SFLVNQTMTAFGTYFVLAFQSVGGSTSHAGIALFIASFTNFPSMLYASKVIKKLGRERTL 267 + T + + +GG S+ G A FI T + ++ +K + Sbjct: 209 IVFITVTHRTNDIFLSIYIVELGGPESYIGWAWFIGVATEAAVLATSTLWFRKFSPIGFV 268 Query: 268 LLGALIYVLRWGIQFAFPTPGVMIGVQVLHGLSFGLFYVAAVEYVSHITLPEMQATGQSV 327 + A+ Y +RW + P +++ +Q LHG++FG+FYVAA +VSH+ +QATG + Sbjct: 269 IFAAVFYGVRWWLVSMVAVPWLLLPLQTLHGMTFGVFYVAAFAFVSHLIPKHLQATGHVL 328 Query: 328 F 328 F Sbjct: 329 F 329 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.138 0.396 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 436,596,462 Number of extensions: 15208652 Number of successful extensions: 53896 Number of sequences better than 1.0e-05: 47 Number of HSP's gapped: 53947 Number of HSP's successfully gapped: 47 Length of query: 393 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 265 Effective length of database: 409,704,906 Effective search space: 108571800090 Effective search space used: 108571800090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 127 (53.5 bits)