BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0004.p (370 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAL17866.1| citrate synthase [Bacillus methanolicus] 437 e-121 ref|NP_244026.1| citrate synthase II [Bacillus halodurans] >gi|2... 432 e-120 ref|NP_390792.1| citrate synthase II [Bacillus subtilis] >gi|312... 430 e-119 gb|AAA96341.1| citrate synthase II 420 e-116 ref|NP_981018.1| citrate synthase CitZ [Bacillus cereus ATCC 109... 410 e-113 >gb|AAL17866.1| citrate synthase [Bacillus methanolicus] Length = 372 Score = 437 bits (1124), Expect = e-121, Method: Compositional matrix adjust. Identities = 211/371 (56%), Positives = 271/371 (73%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MT T+GLEG+VA +D LTY GY+IDDLA++A+FEE +LLW LPT EL+ Sbjct: 1 MTVTRGLEGVVATTSSISSIIDDTLTYVGYNIDDLADNASFEEVIFLLWHRKLPTKSELE 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYP-KEANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L+ L+ A +P++++ K+YP + + M YDE AD M EAN KA Sbjct: 61 ELKILLAENAELPKEILDHFKMYPIDKVHPMAALRSAVSLLGLYDEEADLMDEEANYRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 ++LQA+IP++VAA AR+RKGLEPVAP++ LS A NFLYML GK+P+ ++V+ Sbjct: 121 IRLQAKIPSIVAAFARVRKGLEPVAPRKDLSFAANFLYMLKGKEPEDIAVEAFNKVLVLH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSDIYSGVT+AIGALKGPLHGGANEAVMKML EIG+L+ VEP Sbjct: 181 ADHELNASTFTARVCVATLSDIYSGVTAAIGALKGPLHGGANEAVMKMLTEIGSLENVEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ KL ++EKIMGFGHRVY+ GDPRAKHL +MS +L + + Y+MS K+E ++T + Sbjct: 241 YIREKLAKKEKIMGFGHRVYRKGDPRAKHLREMSKKLTELTGEPHWYEMSTKIEAIVTGE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 KGL PNVDFYSAS+Y LGI+ +LFTPIFA+SR SGW AHILEQYE+NR+IRPRA+Y G Sbjct: 301 KGLPPNVDFYSASIYHSLGIDHDLFTPIFAVSRISGWLAHILEQYENNRLIRPRADYIGP 360 Query: 360 SEQKYVPVDER 370 QK++P+++R Sbjct: 361 GMQKFIPIEQR 371 >ref|NP_244026.1| citrate synthase II [Bacillus halodurans] pir||H84044 citrate synthase II citZ [imported] - Bacillus halodurans (strain C-125) dbj|BAB06879.1| citrate synthase II [Bacillus halodurans] Length = 372 Score = 432 bits (1110), Expect = e-120, Method: Compositional matrix adjust. Identities = 214/371 (57%), Positives = 269/371 (72%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 M+ T+GLEG+VA ++ VLTY+GYDID+LAEHA+FEE YLLW G LP EL+ Sbjct: 1 MSVTRGLEGVVATTSSVSSIIESVLTYQGYDIDELAEHASFEEVIYLLWNGKLPNKVELK 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYP-KEANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L +L+ A IP++VI QMK YP + M YDE AD M AN+ KA Sbjct: 61 ELADELANNAEIPQEVIEQMKAYPLDRVHPMGALRTAISSLGLYDEEADLMDEAANKRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 +KLQAQ+ TVV A +RIR+G EPVAP+ LS A NFLYML G++PD ++V Sbjct: 121 LKLQAQVSTVVTAFSRIREGKEPVAPRTDLSFAANFLYMLTGEEPDEIAVDAFNKALVLH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSD+YSG+T+AIGALKGPLHGGANE VM ML EIG ++ VEP Sbjct: 181 ADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANEQVMAMLTEIGDVENVEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ L+ ++KIMGFGHRVYKNGDPRAKHL +MS +L + ++ Y+MSVK+EE++T++ Sbjct: 241 YIRKALDNKQKIMGFGHRVYKNGDPRAKHLREMSKKLTSITGESKWYEMSVKIEEIVTNE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 KGL PNVDFYSASVY LGI+ +LFTPIFA+SR SGW AHILEQY +NR+IRPRAEY G Sbjct: 301 KGLLPNVDFYSASVYHSLGIKHDLFTPIFAVSRMSGWLAHILEQYSNNRLIRPRAEYVGP 360 Query: 360 SEQKYVPVDER 370 ++++YV ++ER Sbjct: 361 NKRQYVRLEER 371 >ref|NP_390792.1| citrate synthase II [Bacillus subtilis] sp|P39120|CISZ_BACSU Citrate synthase II pir||G69600 citrate (si)-synthase (EC 4.1.3.7) citZ [validated] - Bacillus subtilis gb|AAC00345.1| citrate synthase subunit II [Bacillus subtilis] emb|CAB14874.1| citrate synthase II [Bacillus subtilis subsp. subtilis str. 168] Length = 372 Score = 430 bits (1105), Expect = e-119, Method: Compositional matrix adjust. Identities = 208/371 (56%), Positives = 266/371 (71%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MTAT+GLEG+VA +D LTY GYDIDDL E+A+FEE YLLW LP EL+ Sbjct: 1 MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENASFEEIIYLLWHLRLPNKKELE 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYPKE-ANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L++ L+ A +P+++I K Y E + M D AD M+ EAN KA Sbjct: 61 ELKQQLAKEAAVPQEIIEHFKSYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 ++LQA++P +VAA +RIRKGLEPV P+E +AENFLY L G++P + V+ Sbjct: 121 IRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTLNGEEPSPIEVEAFNKALILH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSDIYSG+T+AIGALKGPLHGGANE VMKML EIG ++ EP Sbjct: 181 ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ KLE++EKIMGFGHRVYK+GDPRAKHL +MS L + ++ Y+MS+++E+++TS+ Sbjct: 241 YIRAKLEKKEKIMGFGHRVYKHGDPRAKHLKEMSKRLTNLTGESKWYEMSIRIEDIVTSE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 K L PNVDFYSASVY LGI+ +LFTPIFA+SR SGW AHILEQY++NR+IRPRA+YTG Sbjct: 301 KKLPPNVDFYSASVYHSLGIDHDLFTPIFAVSRMSGWLAHILEQYDNNRLIRPRADYTGP 360 Query: 360 SEQKYVPVDER 370 +QK+VP++ER Sbjct: 361 DKQKFVPIEER 371 >gb|AAA96341.1| citrate synthase II Length = 372 Score = 420 bits (1079), Expect = e-116, Method: Compositional matrix adjust. Identities = 204/371 (54%), Positives = 262/371 (70%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MTAT+GLEG+VA +D LTY GYDIDDL E+ +FEE YLLW LP EL+ Sbjct: 1 MTATRGLEGVVATTSSVSSIIDDTLTYVGYDIDDLTENRSFEEIIYLLWHLRLPNKKELE 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYPKE-ANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 L++ L+ A +P+++I K Y E + M D AD M+ EAN KA Sbjct: 61 ELKQQLAKEAAVPQEIIEHFKSYSLENVHPMAALRTAISLLGLLDSEADTMNPEANYRKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 ++LQA++P +VAA +RIRKGLEPV P+E +AENFLY L G++P + V+ Sbjct: 121 IRLQAKVPGLVAAFSRIRKGLEPVEPREDYGIAENFLYTLNGEEPSPIEVEAFNKALILH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSDIYSG+T+AIGALKGPLHGGANE VMKML EIG ++ EP Sbjct: 181 ADHELNASTFTARVCVATLSDIYSGITAAIGALKGPLHGGANEGVMKMLTEIGEVENAEP 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI+ KLE++EKIMGFGHRVYK+GDPRA L +MS L + ++ Y+MS+++E+++TS+ Sbjct: 241 YIRAKLEKKEKIMGFGHRVYKHGDPRANDLKEMSKRLTNLTGESKWYEMSIRIEDIVTSE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 K L PNVDFYSASVY LGI+ +LFTPIFA+ R SGW AHILEQY++NR+IRPRA+YTG Sbjct: 301 KKLPPNVDFYSASVYHSLGIDHDLFTPIFAVRRMSGWLAHILEQYDNNRLIRPRADYTGP 360 Query: 360 SEQKYVPVDER 370 +QK+VP++ER Sbjct: 361 DKQKFVPIEER 371 >ref|NP_981018.1| citrate synthase CitZ [Bacillus cereus ATCC 10987] gb|AAS43626.1| citrate synthase CitZ [Bacillus cereus ATCC 10987] Length = 371 Score = 410 bits (1055), Expect = e-113, Method: Compositional matrix adjust. Identities = 203/371 (54%), Positives = 258/371 (69%), Gaps = 1/371 (0%) Query: 1 MTATKGLEGIVAXXXXXXXXVDGVLTYRGYDIDDLAEHATFEETAYLLWFGNLPTTPELQ 60 MT +GLEG+VA +D LTY GY+IDDLAE+ATFEE YLLW LP EL Sbjct: 1 MTVIRGLEGVVATTSSVSSIIDDTLTYVGYNIDDLAENATFEEVVYLLWHRKLPNEQELA 60 Query: 61 ALQRDLSAFAPIPEQVIAQMKLYP-KEANTMXXXXXXXXXXXXYDEAADDMSREANEIKA 119 + +S + +P +++A +K K A+ M YDE+A+ M ++N +KA Sbjct: 61 EFKEIVSEYYKVPGEILAYLKQVDLKIAHPMSVLRTAISMLSLYDESAEIMDEKSNYLKA 120 Query: 120 VKLQAQIPTVVAALARIRKGLEPVAPKEGLSLAENFLYMLWGKQPDIVSVKXXXXXXXXX 179 VKLQAQ+ T+VAA ARIRKGL+ V P+ LSLA NFLYML ++P+ V ++ Sbjct: 121 VKLQAQVGTLVAAYARIRKGLDVVEPRNDLSLAANFLYMLNDREPNEVEIEAFDKALVLH 180 Query: 180 XXXXXNASTFAGRVTVATLSDIYSGVTSAIGALKGPLHGGANEAVMKMLEEIGTLDAVEP 239 NASTF RV VATLSD+YSG+T+AIGALKGPLHGGANE VMKML EIG + VE Sbjct: 181 ADHELNASTFTARVCVATLSDVYSGITAAIGALKGPLHGGANENVMKMLTEIGEEENVES 240 Query: 240 YIQGKLERREKIMGFGHRVYKNGDPRAKHLMKMSHELGLMKNDTALYDMSVKVEELITSK 299 YI L+ + KIMGFGHRVY++GDPRAKHL +MS L ++ + Y+MS+K+E+++T + Sbjct: 241 YIHNALQNKVKIMGFGHRVYEHGDPRAKHLREMSKRLCVLLGEEKWYNMSIKIEDIVTKE 300 Query: 300 KGLKPNVDFYSASVYTQLGIERELFTPIFAISRTSGWTAHILEQYEDNRIIRPRAEYTGM 359 KGL PNVDFYSASVY LGI+ +LFTPIFAISR SGW AHILEQYE+NR+IRPRA+Y G Sbjct: 301 KGLPPNVDFYSASVYHCLGIDHDLFTPIFAISRMSGWLAHILEQYENNRLIRPRADYNGP 360 Query: 360 SEQKYVPVDER 370 + Q YVP+ +R Sbjct: 361 THQVYVPIAQR 371 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.133 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 469,115,732 Number of extensions: 17366554 Number of successful extensions: 42970 Number of sequences better than 1.0e-05: 1243 Number of HSP's gapped: 40906 Number of HSP's successfully gapped: 1249 Length of query: 370 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 243 Effective length of database: 412,420,005 Effective search space: 100218061215 Effective search space used: 100218061215 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)