BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0333.0003.p (431 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAA73794.1| isocitrate dehydrogenase [Bacillus israeli] 598 e-170 ref|NP_390791.1| isocitrate dehydrogenase [Bacillus subtilis] >g... 595 e-169 pdb|1HQS|A Chain A, Crystal Structure Of Isocitrate Dehydrogenas... 591 e-167 ref|NP_470937.1| highly similar to isocitrate dehyrogenases [Lis... 589 e-167 ref|NP_465091.1| highly similar to isocitrate dehyrogenases [Lis... 587 e-166 >emb|CAA73794.1| isocitrate dehydrogenase [Bacillus israeli] Length = 425 Score = 598 bits (1543), Expect = e-170, Method: Compositional matrix adjust. Identities = 287/420 (68%), Positives = 332/420 (79%) Query: 12 EGEQITIEDGKLLVPDHPIIPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVF 71 EG++IT+ +G L VP+ PIIPFIEGDGTG DIW+A+ +VL+AAV KAY G+K+I W EV Sbjct: 5 EGQKITMTNGTLNVPNDPIIPFIEGDGTGPDIWRAASKVLEAAVEKAYNGEKKIVWKEVL 64 Query: 72 AGEKAFNTYGEWLPNDTLEAIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPV 131 AGEKAF GEWLP +TL+ IREY++AIKGPLTTP+GGGIRSLNVALRQELDL+VCLRPV Sbjct: 65 AGEKAFKETGEWLPQETLDVIREYMIAIKGPLTTPVGGGIRSLNVALRQELDLFVCLRPV 124 Query: 132 RYFDGVPSPVKHPELVDMVIFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFP 191 RYF+GVPSPVK P+ DMVIFRENTEDIYAGIEY +G+ EVKKVIEFLQ ELGVNKIRFP Sbjct: 125 RYFNGVPSPVKRPQDTDMVIFRENTEDIYAGIEYAKGTEEVKKVIEFLQKELGVNKIRFP 184 Query: 192 ETSGIGIKPVSSEGSKRLVRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQ 251 ETSGIGIKP+S EG+KRLVR+A+ YAI+HGRKSVTLVHKGNIMKFTEGAFKNWGYE+AEQ Sbjct: 185 ETSGIGIKPISEEGTKRLVRAAINYAIEHGRKSVTLVHKGNIMKFTEGAFKNWGYELAEQ 244 Query: 252 EFGDKVFTWNQYDAIXXXXXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDV 311 EFGDKVFTW +YD I +G LQQ+LTRP +FDV Sbjct: 245 EFGDKVFTWAEYDRIVEKEGKEAANKAQEEAEKAGKIIIKDAIADIFLQQILTRPREFDV 304 Query: 312 IATLNLNGDYLSDALXXXXXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSV 371 +AT+NLNGDY+SDAL NY TGHAIFEATHGTAPKYA D VNP SV Sbjct: 305 VATMNLNGDYISDALAAQVGGIGIAPGANINYETGHAIFEATHGTAPKYAGLDKVNPSSV 364 Query: 372 ILSGVMLLEHLGWQEAADLIYKGMSTAINNKTVTYDFARQMEGATELKCSAFADEIISHL 431 ILSGV++LEHLGW+EAADLI + M I +K VTYDFAR M+GATE+KCS F +E+I ++ Sbjct: 365 ILSGVLMLEHLGWKEAADLIIQAMEKTIASKVVTYDFARLMDGATEVKCSEFGNELIKNM 424 >ref|NP_390791.1| isocitrate dehydrogenase [Bacillus subtilis] sp|P39126|IDH_BACSU Isocitrate dehydrogenase [NADP] (Oxalosuccinate decarboxylase) (IDH) (NADP+-specific ICDH) (IDP) pir||I40382 isocitrate dehydrogenase (NADP) (EC 1.1.1.42) - Bacillus subtilis gb|AAA96342.1| isocitrate dehydrogenase gb|AAC00346.1| isocitrate dehydrogenase [Bacillus subtilis] emb|CAB14873.1| isocitrate dehydrogenase [Bacillus subtilis subsp. subtilis str. 168] Length = 423 Score = 595 bits (1534), Expect = e-169, Method: Compositional matrix adjust. Identities = 286/420 (68%), Positives = 330/420 (78%) Query: 12 EGEQITIEDGKLLVPDHPIIPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVF 71 +GE+IT+ +G L VP++PIIPFIEGDGTG DIW A+ +VL+AAV KAY G+K+I W EV+ Sbjct: 3 QGEKITVSNGVLNVPNNPIIPFIEGDGTGPDIWNAASKVLEAAVEKAYKGEKKITWKEVY 62 Query: 72 AGEKAFNTYGEWLPNDTLEAIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPV 131 AGEKA+N GEWLP +TL+ IREY +AIKGPLTTP+GGGIRSLNVALRQELDL+VCLRPV Sbjct: 63 AGEKAYNKTGEWLPAETLDVIREYFIAIKGPLTTPVGGGIRSLNVALRQELDLFVCLRPV 122 Query: 132 RYFDGVPSPVKHPELVDMVIFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFP 191 RYF GVPSPVK PE DMVIFRENTEDIYAGIEY +GS EV+K+I FLQNEL VNKIRFP Sbjct: 123 RYFTGVPSPVKRPEDTDMVIFRENTEDIYAGIEYAKGSEEVQKLISFLQNELNVNKIRFP 182 Query: 192 ETSGIGIKPVSSEGSKRLVRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQ 251 ETSGIGIKPVS EG+ RLVR+A++YAI+HGRKSVTLVHKGNIMKFTEGAFKNWGYE+AE+ Sbjct: 183 ETSGIGIKPVSEEGTSRLVRAAIDYAIEHGRKSVTLVHKGNIMKFTEGAFKNWGYELAEK 242 Query: 252 EFGDKVFTWNQYDAIXXXXXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDV 311 E+GDKVFTW QYD I +G LQQ+LTRP +FDV Sbjct: 243 EYGDKVFTWAQYDRIAEEQGKDAANKAQSEAEAAGKIIIKDSIADIFLQQILTRPNEFDV 302 Query: 312 IATLNLNGDYLSDALXXXXXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSV 371 +AT+NLNGDY+SDAL NY TGHAIFEATHGTAPKYA D VNP SV Sbjct: 303 VATMNLNGDYISDALAAQVGGIGIAPGANINYETGHAIFEATHGTAPKYAGLDKVNPSSV 362 Query: 372 ILSGVMLLEHLGWQEAADLIYKGMSTAINNKTVTYDFARQMEGATELKCSAFADEIISHL 431 ILSGV+LLEHLGW EAADL+ K M I +K VTYDFAR M+GATE+KCS F +E+I ++ Sbjct: 363 ILSGVLLLEHLGWNEAADLVIKSMEKTIASKVVTYDFARLMDGATEVKCSEFGEELIKNM 422 >pdb|1HQS|A Chain A, Crystal Structure Of Isocitrate Dehydrogenase From Bacillus Subtilis pdb|1HQS|B Chain B, Crystal Structure Of Isocitrate Dehydrogenase From Bacillus Subtilis Length = 423 Score = 591 bits (1523), Expect = e-167, Method: Compositional matrix adjust. Identities = 285/420 (67%), Positives = 329/420 (78%) Query: 12 EGEQITIEDGKLLVPDHPIIPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVF 71 +GE+IT+ +G L VP++PIIPFIEGDGTG DIW A+ +VL+AAV KAY G+K+I W EV+ Sbjct: 3 QGEKITVSNGVLNVPNNPIIPFIEGDGTGPDIWNAASKVLEAAVEKAYKGEKKITWKEVY 62 Query: 72 AGEKAFNTYGEWLPNDTLEAIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPV 131 AGEKA+N GEWLP +TL+ IREY +AIKGPLTTP+GGGIRSLNVALRQELDL+V LRPV Sbjct: 63 AGEKAYNKTGEWLPAETLDVIREYFIAIKGPLTTPVGGGIRSLNVALRQELDLFVXLRPV 122 Query: 132 RYFDGVPSPVKHPELVDMVIFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFP 191 RYF GVPSPVK PE DMVIFRENTEDIYAGIEY +GS EV+K+I FLQNEL VNKIRFP Sbjct: 123 RYFTGVPSPVKRPEDTDMVIFRENTEDIYAGIEYAKGSEEVQKLISFLQNELNVNKIRFP 182 Query: 192 ETSGIGIKPVSSEGSKRLVRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQ 251 ETSGIGIKPVS EG+ RLVR+A++YAI+HGRKSVTLVHKGNIMKFTEGAFKNWGYE+AE+ Sbjct: 183 ETSGIGIKPVSEEGTSRLVRAAIDYAIEHGRKSVTLVHKGNIMKFTEGAFKNWGYELAEK 242 Query: 252 EFGDKVFTWNQYDAIXXXXXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDV 311 E+GDKVFTW QYD I +G LQQ+LTRP +FDV Sbjct: 243 EYGDKVFTWAQYDRIAEEQGKDAANKAQSEAEAAGKIIIKDSIADIFLQQILTRPNEFDV 302 Query: 312 IATLNLNGDYLSDALXXXXXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSV 371 +AT+NLNGDY+SDAL NY TGHAIFEATHGTAPKYA D VNP SV Sbjct: 303 VATMNLNGDYISDALAAQVGGIGIAPGANINYETGHAIFEATHGTAPKYAGLDKVNPSSV 362 Query: 372 ILSGVMLLEHLGWQEAADLIYKGMSTAINNKTVTYDFARQMEGATELKCSAFADEIISHL 431 ILSGV+LLEHLGW EAADL+ K M I +K VTYDFAR M+GATE+KCS F +E+I ++ Sbjct: 363 ILSGVLLLEHLGWNEAADLVIKSMEKTIASKVVTYDFARLMDGATEVKCSEFGEELIKNM 422 >ref|NP_470937.1| highly similar to isocitrate dehyrogenases [Listeria innocua] pir||AH1632 isocitrate dehyrogenases homolog citC [imported] - Listeria innocua (strain Clip11262) emb|CAC96832.1| citC [Listeria innocua] Length = 420 Score = 589 bits (1518), Expect = e-167, Method: Compositional matrix adjust. Identities = 284/418 (67%), Positives = 327/418 (78%) Query: 14 EQITIEDGKLLVPDHPIIPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAG 73 ++I +E+G L P++P+IPFIEGDGTG DIW A+KRVLDAAV KAY G+K+IAW EV AG Sbjct: 3 QKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVLAG 62 Query: 74 EKAFNTYGEWLPNDTLEAIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRY 133 EKAF GEWLP +TL+ I EY++AIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRY Sbjct: 63 EKAFKQTGEWLPQETLDTIDEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRY 122 Query: 134 FDGVPSPVKHPELVDMVIFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFPET 193 F GVPSPVK PE DMVIFRENTEDIYAGIE+KEGSAE KK+I+FL++E GV+KIRFPET Sbjct: 123 FKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSAESKKLIDFLKSEFGVDKIRFPET 182 Query: 194 SGIGIKPVSSEGSKRLVRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEF 253 SGIGIKP+S EG++RLVRSA+EYAIK GRKS+TLVHKGNIMKFTEGAFKNWGY++ E+E+ Sbjct: 183 SGIGIKPISKEGTERLVRSAIEYAIKEGRKSLTLVHKGNIMKFTEGAFKNWGYDLCEREY 242 Query: 254 GDKVFTWNQYDAIXXXXXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIA 313 GDKVFTWN+YD I +G LQQ+LTRP +FDV+A Sbjct: 243 GDKVFTWNEYDRIKEAEGTEAADAKQKEELAAGKILVKDSIADIFLQQILTRPAEFDVVA 302 Query: 314 TLNLNGDYLSDALXXXXXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVIL 373 T+NLNGDY+SDAL NY+TGHAIFEATHGTAPKYA D VNP SVIL Sbjct: 303 TMNLNGDYISDALAAQVGGIGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSVIL 362 Query: 374 SGVMLLEHLGWQEAADLIYKGMSTAINNKTVTYDFARQMEGATELKCSAFADEIISHL 431 SG +LLEH+GW EAA LI M I KTVTYDFAR M+GATE+KCS FADE+I + Sbjct: 363 SGTLLLEHIGWGEAAALINHSMEKTIAAKTVTYDFARLMDGATEVKCSEFADELIKNF 420 >ref|NP_465091.1| highly similar to isocitrate dehyrogenases [Listeria monocytogenes EGD-e] pir||AF1270 isocitrate dehyrogenases homolog citC [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC99644.1| citC [Listeria monocytogenes] Length = 420 Score = 587 bits (1513), Expect = e-166, Method: Compositional matrix adjust. Identities = 283/418 (67%), Positives = 326/418 (77%) Query: 14 EQITIEDGKLLVPDHPIIPFIEGDGTGRDIWKASKRVLDAAVAKAYGGKKQIAWYEVFAG 73 ++I +E+G L P++P+IPFIEGDGTG DIW A+KRVLDAAV KAY G+K+IAW EV AG Sbjct: 3 QKIQVENGVLKTPNNPVIPFIEGDGTGPDIWAAAKRVLDAAVKKAYNGEKEIAWKEVLAG 62 Query: 74 EKAFNTYGEWLPNDTLEAIREYIVAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRY 133 EKAF GEWLP TL+ I EY++AIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRY Sbjct: 63 EKAFKQTGEWLPQATLDTIDEYLIAIKGPLTTPIGGGIRSLNVALRQELDLYVCLRPVRY 122 Query: 134 FDGVPSPVKHPELVDMVIFRENTEDIYAGIEYKEGSAEVKKVIEFLQNELGVNKIRFPET 193 F GVPSPVK PE DMVIFRENTEDIYAGIE+KEGS E KK+I+FL++E GV+KIRFPET Sbjct: 123 FKGVPSPVKRPEDTDMVIFRENTEDIYAGIEFKEGSIESKKLIDFLKSEFGVDKIRFPET 182 Query: 194 SGIGIKPVSSEGSKRLVRSAVEYAIKHGRKSVTLVHKGNIMKFTEGAFKNWGYEVAEQEF 253 SGIGIKP+S EG++RLVRSA+EYAIK GRKS+TLVHKGNIMKFTEGAFKNWGY++ E+E+ Sbjct: 183 SGIGIKPISKEGTERLVRSAIEYAIKEGRKSLTLVHKGNIMKFTEGAFKNWGYDLCEREY 242 Query: 254 GDKVFTWNQYDAIXXXXXXXXXXXXXXXXXXSGXXXXXXXXXXXXLQQVLTRPTDFDVIA 313 GDKVFTWN+YD I +G LQQ+LTRP +FDV+A Sbjct: 243 GDKVFTWNEYDRIKEAEGTEVADAKQNEALAAGKILVKDSIADIFLQQILTRPAEFDVVA 302 Query: 314 TLNLNGDYLSDALXXXXXXXXXXXXXXXNYVTGHAIFEATHGTAPKYADKDVVNPGSVIL 373 T+NLNGDY+SDAL NY+TGHAIFEATHGTAPKYA D VNP SVIL Sbjct: 303 TMNLNGDYISDALAAQVGGIGIAPGANINYLTGHAIFEATHGTAPKYAGLDKVNPSSVIL 362 Query: 374 SGVMLLEHLGWQEAADLIYKGMSTAINNKTVTYDFARQMEGATELKCSAFADEIISHL 431 SG +LLEH+GW EAA LI + M I KTVTYDFAR M+GATE+KCS FADE+I + Sbjct: 363 SGTLLLEHIGWGEAAALINQSMEKTIAAKTVTYDFARLMDGATEVKCSEFADELIKNF 420 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.137 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 599,175,881 Number of extensions: 24045812 Number of successful extensions: 60204 Number of sequences better than 1.0e-05: 889 Number of HSP's gapped: 59256 Number of HSP's successfully gapped: 1188 Length of query: 431 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 303 Effective length of database: 409,704,906 Effective search space: 124140586518 Effective search space used: 124140586518 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)