BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0331.0007.p (218 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_102624.1| hypothetical protein mlr0926 [Mesorhizobium lot... 142 4e-33 ref|NP_627042.1| conserved hypothetical protein [Streptomyces co... 102 5e-21 ref|ZP_00118090.1| COG0262: Dihydrofolate reductase [Cytophaga h... 97 2e-19 ref|NP_824420.1| hypothetical protein [Streptomyces avermitilis ... 93 5e-18 ref|NP_821646.1| hypothetical protein [Streptomyces avermitilis ... 90 4e-17 >ref|NP_102624.1| hypothetical protein mlr0926 [Mesorhizobium loti MAFF303099] dbj|BAB48410.1| hypothetical protein [Mesorhizobium loti] Length = 188 Score = 142 bits (359), Expect = 4e-33, Method: Compositional matrix adjust. Identities = 75/155 (48%), Positives = 99/155 (63%), Gaps = 5/155 (3%) Query: 1 MAKIVFALNQSLDGFV---DHDHPAFQPDPVLFRHFIDDVRGLAGSLYGRRMYEIMRYWD 57 MAKIV+++ SLDG++ D D P+ L +HF D +R + L GRRMYE MR+WD Sbjct: 1 MAKIVYSMLTSLDGYIAGPDGDIGLPVPETELHQHFNDMMRQASAVLAGRRMYETMRFWD 60 Query: 58 GDHMEWDAPE--REYAAAWQSKPKWVVSRTLKSVGPNASLIEGDLAAAIRGLKAQHEGEI 115 E A E R++A AW+ PK V S TL+ VG NA L+ GD A+R LK + +G + Sbjct: 61 SPDRETGATEVERDFADAWRDTPKIVFSTTLRQVGSNARLVNGDAETAVRSLKTKTDGLV 120 Query: 116 EVAGPELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150 + G +LA LT GL+DEYRLY+HPVVLG GKP+ Sbjct: 121 ILGGADLAADLTRAGLIDEYRLYMHPVVLGGGKPY 155 >ref|NP_627042.1| conserved hypothetical protein [Streptomyces coelicolor A3(2)] emb|CAC44276.1| conserved hypothetical protein [Streptomyces coelicolor A3(2)] Length = 188 Score = 102 bits (254), Expect = 5e-21, Method: Compositional matrix adjust. Identities = 64/157 (40%), Positives = 90/157 (57%), Gaps = 7/157 (4%) Query: 1 MAKIVFALNQSLDGFVDHDHPAFQ---PDPVLFRHFIDDVRGLAGSLYGRRMYEIMRYWD 57 M ++ +++N SLDG++ H A P +FR + D++R L GRR+YE M YW+ Sbjct: 1 MRRVTYSMNVSLDGYIVDPHGAIDWADPAEEVFRFWTDEIRETGAHLLGRRLYETMLYWE 60 Query: 58 -GDHME-WDAPEREYAAAWQSKPKWVVSRTLKSV-GPNASLIEGDLAAAIRGLKAQ-HEG 113 D E DA E E+AA W++ PK V SRTL +V G N L G LA I L+A+ +G Sbjct: 61 TADRDESLDAAELEWAALWKALPKVVFSRTLTTVQGTNTRLASGTLAEEIERLRAEPGDG 120 Query: 114 EIEVAGPELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150 +I + LA L+DEYR +HPV++G G PF Sbjct: 121 DIAIGAATLAAEAAASDLIDEYRTMVHPVLVGGGTPF 157 >ref|ZP_00118090.1| COG0262: Dihydrofolate reductase [Cytophaga hutchinsonii] Length = 182 Score = 97.4 bits (241), Expect = 2e-19, Method: Compositional matrix adjust. Identities = 55/153 (35%), Positives = 82/153 (53%), Gaps = 5/153 (3%) Query: 1 MAKIVFALNQSLDGFVDHDHPAFQPDPVLFRHFIDDVRGLAGSLYGRRMYEIMRYWDGDH 60 M K++ A+N ++DG+ DH A PD + +H+ D + ++YGR Y++M+YW Sbjct: 1 MRKVIAAINMTIDGYCDHT--AVNPDAEIHQHYADLLHNTGVAVYGRVTYQLMQYWQSIR 58 Query: 61 MEWDAPER--EYAAAWQSKPKWVVSRTLKSVGPN-ASLIEGDLAAAIRGLKAQHEGEIEV 117 + E+AAA PK V S TLK G N A+L +G L + LK Q +I V Sbjct: 59 ANPTGKKATDEFAAAIDRVPKLVFSHTLKDTGWNSATLAKGSLEEELTALKQQPGKDIYV 118 Query: 118 AGPELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150 P + + L L+DE++L +HPVV G G P Sbjct: 119 CSPGMIVAAMNLNLIDEFQLCVHPVVAGSGLPL 151 >ref|NP_824420.1| hypothetical protein [Streptomyces avermitilis MA-4680] dbj|BAC70955.1| hypothetical protein [Streptomyces avermitilis MA-4680] Length = 215 Score = 92.8 bits (229), Expect = 5e-18, Method: Compositional matrix adjust. Identities = 61/181 (33%), Positives = 91/181 (50%), Gaps = 8/181 (4%) Query: 1 MAKIVFALNQSLDGFVDHDHPAFQ---PDPVLFRHFIDDVRGLAGSLYGRRMYEIM-RYW 56 M KIV ++ SLDG+++ + D + +HF D +RG+ L GR Y++M +W Sbjct: 1 MRKIVLMMSVSLDGYIEGPNREIDWHLVDDEVHQHFNDTLRGMGAFLSGRVTYQLMADFW 60 Query: 57 ---DGDHMEWDAPEREYAAAWQSKPKWVVSRTLKSVGPNASLIEGDLAAAIRGLKAQHEG 113 D D P ++A W+ PK V SRTL+ G + +++ + I LKAQ G Sbjct: 61 PTADADPAS-TGPVADFARIWRDMPKIVFSRTLQHAGWHTTIVRDLVVEDIHALKAQEGG 119 Query: 114 EIEVAGPELAHSLTELGLVDEYRLYLHPVVLGHGKPFXXXXAAAAPRGKRSNWREGNQVD 173 ++ + G +LA LVDEYR+Y+HPV+LG GK P + GN V Sbjct: 120 DLALGGADLAAEFMRHDLVDEYRVYVHPVLLGRGKRLFPETDTLTPLRLTESRTFGNGVA 179 Query: 174 L 174 L Sbjct: 180 L 180 >ref|NP_821646.1| hypothetical protein [Streptomyces avermitilis MA-4680] dbj|BAC68181.1| hypothetical protein [Streptomyces avermitilis MA-4680] Length = 188 Score = 89.7 bits (221), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 59/157 (37%), Positives = 86/157 (54%), Gaps = 7/157 (4%) Query: 1 MAKIVFALNQSLDGFV----DHDHPAFQPDPVLFRHFIDDVRGLAGSLYGRRMYEIMR-Y 55 M K+++ +N +LDG++ D + P LF+ ++D R ++ LYGR+++E M Y Sbjct: 1 MRKLIYGMNLTLDGYIAAAGDDIGWSGPPSDELFQWWLDQERAISLLLYGRKLWETMSAY 60 Query: 56 W-DGDHMEWDAP-EREYAAAWQSKPKWVVSRTLKSVGPNASLIEGDLAAAIRGLKAQHEG 113 W GD P E E+A W+ PK V S T+ V N L+ GD A I LKA+ G Sbjct: 61 WPTGDQQPNATPAEIEFARNWRDTPKVVFSSTVDKVDWNTRLVSGDAVAEITRLKAEDGG 120 Query: 114 EIEVAGPELAHSLTELGLVDEYRLYLHPVVLGHGKPF 150 + V G LA + GL+DEY + HPV++G G PF Sbjct: 121 PMRVGGAALAGAAMRAGLIDEYAIVTHPVLVGSGTPF 157 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.137 0.424 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 336,957,731 Number of extensions: 13532817 Number of successful extensions: 36230 Number of sequences better than 1.0e-05: 19 Number of HSP's gapped: 36228 Number of HSP's successfully gapped: 19 Length of query: 218 Length of database: 757,237,578 Length adjustment: 121 Effective length of query: 97 Effective length of database: 428,710,599 Effective search space: 41584928103 Effective search space used: 41584928103 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 123 (52.0 bits)