BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0331.0004.p (177 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAQ87064.1| Transposase [Rhizobium sp. NGR234] 263 2e-69 ref|NP_881015.1| conserved hypothetical protein [Bordetella pert... 233 1e-60 gb|EAJ13778.1| unknown [environmental sequence] 230 1e-59 gb|EAG99087.1| unknown [environmental sequence] 179 3e-44 ref|ZP_00082921.1| COG2128: Uncharacterized conserved protein [P... 155 5e-37 >gb|AAQ87064.1| Transposase [Rhizobium sp. NGR234] Length = 159 Score = 263 bits (671), Expect = 2e-69, Method: Compositional matrix adjust. Identities = 122/159 (76%), Positives = 138/159 (86%) Query: 19 MAQRINYMQQSPEFSKKMMELSNAEKESSIEEKIRHLVHIRASQMNGCGFCLDMHVKEAK 78 M QR+NY QQSPE KK+ +LS A K+S IE+KI LV IRASQ+NGCGFCLDMHVKEAK Sbjct: 1 MTQRLNYAQQSPELFKKLADLSMALKDSVIEQKIHDLVQIRASQINGCGFCLDMHVKEAK 60 Query: 79 IHGESELRLYHISIWRESTLFSPRERAALAWTEVLTKLPEHGVSDDIYDRVRGQLSEKEI 138 IHGESELRLYHI+IWRES LF PRERAALAWTE LT+LPE G+ D++Y+RVRGQLSEKEI Sbjct: 61 IHGESELRLYHIAIWRESNLFVPRERAALAWTEALTRLPEGGIPDELYERVRGQLSEKEI 120 Query: 139 SDLTFSIMAINAWNRVNVAFKNVPGSADAAFGLTKAGLS 177 SDLTFSIM INAWNR ++AFKNVPGSAD +GL KAGL+ Sbjct: 121 SDLTFSIMVINAWNRASIAFKNVPGSADKLYGLDKAGLN 159 >ref|NP_881015.1| conserved hypothetical protein [Bordetella pertussis Tohama I] ref|NP_885415.1| conserved hypothetical protein [Bordetella parapertussis 12822] ref|NP_890234.1| conserved hypothetical protein [Bordetella bronchiseptica RB50] emb|CAE42653.1| conserved hypothetical protein [Bordetella pertussis] emb|CAE38533.1| conserved hypothetical protein [Bordetella parapertussis] emb|CAE35673.1| conserved hypothetical protein [Bordetella bronchiseptica] Length = 159 Score = 233 bits (595), Expect = 1e-60, Method: Compositional matrix adjust. Identities = 109/159 (68%), Positives = 130/159 (81%) Query: 19 MAQRINYMQQSPEFSKKMMELSNAEKESSIEEKIRHLVHIRASQMNGCGFCLDMHVKEAK 78 M QR++Y QQSPE KK + S A K+SSIE IR LV IRASQ+NGC FCLDMHVKEA Sbjct: 1 MTQRLDYQQQSPELFKKFVAFSLALKQSSIETSIRDLVDIRASQINGCTFCLDMHVKEAT 60 Query: 79 IHGESELRLYHISIWRESTLFSPRERAALAWTEVLTKLPEHGVSDDIYDRVRGQLSEKEI 138 +HGE LRL+HI+ WRESTLFSPRERAALAWTEVLT++P G+SD+I+ +VR QLSE+E+ Sbjct: 61 MHGERPLRLHHIAAWRESTLFSPRERAALAWTEVLTRIPAEGISDEIHAQVREQLSEQEL 120 Query: 139 SDLTFSIMAINAWNRVNVAFKNVPGSADAAFGLTKAGLS 177 SDLTF +M+INAWNRVNVAF+ PG D AFG+ K+GLS Sbjct: 121 SDLTFLVMSINAWNRVNVAFRIEPGKYDQAFGVDKSGLS 159 >gb|EAJ13778.1| unknown [environmental sequence] Length = 159 Score = 230 bits (587), Expect = 1e-59, Method: Compositional matrix adjust. Identities = 103/158 (65%), Positives = 128/158 (81%) Query: 19 MAQRINYMQQSPEFSKKMMELSNAEKESSIEEKIRHLVHIRASQMNGCGFCLDMHVKEAK 78 M+QRINY QQSPEF++K++EL ++++IE +R LV IRASQ+NGC FC+DMHVK A+ Sbjct: 1 MSQRINYFQQSPEFTRKLVELDGLFQKTTIEAPVRELVEIRASQLNGCAFCVDMHVKMAR 60 Query: 79 IHGESELRLYHISIWRESTLFSPRERAALAWTEVLTKLPEHGVSDDIYDRVRGQLSEKEI 138 IHGE ELR++H++IWRESTLFSPRERAALAWTE LT L G+ DD+Y+RVR SEKE+ Sbjct: 61 IHGERELRMHHVAIWRESTLFSPRERAALAWTEALTHLSSDGLPDDLYERVRAHYSEKEL 120 Query: 139 SDLTFSIMAINAWNRVNVAFKNVPGSADAAFGLTKAGL 176 SDLTF + AIN WNR+NVAF+ VPGS D FGL KA + Sbjct: 121 SDLTFLVGAINCWNRLNVAFRIVPGSFDKTFGLDKANM 158 >gb|EAG99087.1| unknown [environmental sequence] Length = 159 Score = 179 bits (454), Expect = 3e-44, Method: Compositional matrix adjust. Identities = 81/156 (51%), Positives = 114/156 (73%) Query: 19 MAQRINYMQQSPEFSKKMMELSNAEKESSIEEKIRHLVHIRASQMNGCGFCLDMHVKEAK 78 M+ R+N+ Q P +K +++ S A ++SI+ I+ L+ IRASQ+N C FCLDMH+K+AK Sbjct: 1 MSNRVNHFQTVPNLTKALIDASMASYKTSIDGLIKSLIDIRASQLNSCAFCLDMHIKQAK 60 Query: 79 IHGESELRLYHISIWRESTLFSPRERAALAWTEVLTKLPEHGVSDDIYDRVRGQLSEKEI 138 I GE ELRL+H++IWRES LF+ +E+AALA TE LT +P G+SD++Y + S+ EI Sbjct: 61 IKGEGELRLHHVAIWRESPLFNAKEKAALALTEALTHIPAIGISDELYRSTKEHFSDVEI 120 Query: 139 SDLTFSIMAINAWNRVNVAFKNVPGSADAAFGLTKA 174 S+LT++I IN WNR+ + + PGS DAAFGLTKA Sbjct: 121 SELTYAIGLINFWNRMQILAQMEPGSQDAAFGLTKA 156 >ref|ZP_00082921.1| COG2128: Uncharacterized conserved protein [Pseudomonas fluorescens PfO-1] Length = 146 Score = 155 bits (391), Expect = 5e-37, Method: Compositional matrix adjust. Identities = 69/145 (47%), Positives = 97/145 (66%) Query: 19 MAQRINYMQQSPEFSKKMMELSNAEKESSIEEKIRHLVHIRASQMNGCGFCLDMHVKEAK 78 M+ R++Y SP+ K M+ L SIE + HL+ IRASQ+NGC FC DMH EA+ Sbjct: 1 MSPRLDYYSASPKAMKAMIGLEALTSNLSIEPPLLHLIKIRASQLNGCAFCTDMHSVEAR 60 Query: 79 IHGESELRLYHISIWRESTLFSPRERAALAWTEVLTKLPEHGVSDDIYDRVRGQLSEKEI 138 GE++ RLY I++WR+S F+ RERAALAWTE +T L + V DD+Y + R Q +E E+ Sbjct: 61 RAGETDRRLYAIAVWRDSGFFNLRERAALAWTEAVTLLADSRVPDDVYQQAREQFNETEL 120 Query: 139 SDLTFSIMAINAWNRVNVAFKNVPG 163 DLT ++ IN+WNR+ V+F+ +P Sbjct: 121 VDLTIAVSTINSWNRLAVSFRQIPS 145 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.130 0.376 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 242,960,972 Number of extensions: 8565895 Number of successful extensions: 23930 Number of sequences better than 1.0e-05: 96 Number of HSP's gapped: 23917 Number of HSP's successfully gapped: 96 Length of query: 177 Length of database: 757,237,578 Length adjustment: 117 Effective length of query: 60 Effective length of database: 439,570,995 Effective search space: 26374259700 Effective search space used: 26374259700 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 122 (51.6 bits)