BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0331.0003.p (246 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00184184.1| hypothetical protein Exigu032901 [Exiguobacte... 269 4e-71 ref|ZP_00086953.1| COG4221: Short-chain alcohol dehydrogenase of... 260 2e-68 ref|ZP_00124864.1| COG4221: Short-chain alcohol dehydrogenase of... 259 3e-68 gb|EAI95436.1| unknown [environmental sequence] 259 3e-68 gb|EAF52543.1| unknown [environmental sequence] 257 1e-67 >ref|ZP_00184184.1| hypothetical protein Exigu032901 [Exiguobacterium sp. 255-15] Length = 242 Score = 269 bits (687), Expect = 4e-71, Method: Compositional matrix adjust. Identities = 143/241 (59%), Positives = 186/241 (77%), Gaps = 2/241 (0%) Query: 4 IQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSVKAD 63 I+NKVVIITGASSGIGEATAKLLA+ GA++VLAARRE+RL + EI+Q GG+AV D Sbjct: 2 IENKVVIITGASSGIGEATAKLLAKQGAQLVLAARREDRLKTLQQEIEQLGGKAVYQVTD 61 Query: 64 VISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLYGIA 123 V + +++LAK A +T+G +DV VNNAG+MP S+L++ + EW+ M+DVNIKGVLYGI Sbjct: 62 VTDSAQVEQLAKLAQDTFGSVDVLVNNAGLMPLSKLNKNKQDEWNTMVDVNIKGVLYGIG 121 Query: 124 AVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIRSTL 183 AVLP MREQ+ GHIIN+SS AG+ V P++AVY+ TKFAVRAI+EGLR EES + IR+T+ Sbjct: 122 AVLPYMREQKRGHIINISSVAGHDVMPSSAVYSGTKFAVRAITEGLRKEESVENNIRATI 181 Query: 184 IAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMVRPT 243 I+PG TEL D IT E++ L + +IARAIA+A+NEPDD ++EI++RPT Sbjct: 182 ISPGAVDTELKDHITDEEIKQGIGDLK--AMDADAIARAIAYAVNEPDDVAVNEILIRPT 239 Query: 244 A 244 A Sbjct: 240 A 240 >ref|ZP_00086953.1| COG4221: Short-chain alcohol dehydrogenase of unknown specificity [Pseudomonas fluorescens PfO-1] Length = 252 Score = 260 bits (665), Expect = 2e-68, Method: Compositional matrix adjust. Identities = 132/246 (53%), Positives = 172/246 (69%), Gaps = 3/246 (1%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M NIQNKVV+ITGASSGIGEA A+L+A GA VVL ARR ERL+ +V+EI+ GG A + Sbjct: 9 MSNIQNKVVLITGASSGIGEAAARLIAAKGAHVVLGARRTERLDKLVSEIQAEGGSASAC 68 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 DV + M FA +G++DV +NNAGVMP S L L+V EW+QM+DVN++GVL+ Sbjct: 69 ALDVTDLQSMHAFVAFAKTQHGKVDVIINNAGVMPLSPLASLKVDEWNQMLDVNVRGVLH 128 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 GIAAVLP M+ Q G +IN+SS G VSPT AVY ATKFAVRAIS+GLR E +IR Sbjct: 129 GIAAVLPDMQAQGFGQVINISSIGGLAVSPTAAVYCATKFAVRAISDGLRQE---TDKIR 185 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 T++ PG+ ++EL D+I+ + V ++ +IARA+ +AI +PDD +SEI+V Sbjct: 186 VTVVCPGVVESELADTISDEVAREEMKAFRRVALNADAIARALTYAIEQPDDVDVSEIVV 245 Query: 241 RPTALP 246 RPTA P Sbjct: 246 RPTASP 251 >ref|ZP_00124864.1| COG4221: Short-chain alcohol dehydrogenase of unknown specificity [Pseudomonas syringae pv. syringae B728a] Length = 252 Score = 259 bits (663), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 133/243 (54%), Positives = 169/243 (69%), Gaps = 3/243 (1%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M NIQ KVV+ITGASSGIGEA A+L+A GA VVL ARR ERL + +I+ GG A Sbjct: 9 MSNIQGKVVLITGASSGIGEAAARLIAAKGAHVVLGARRSERLQTLAADIEAQGGSARFR 68 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 DV A DMQ A FA + +G+IDV +NNAGVMP S L L++ EW+QM+DVN++GVL+ Sbjct: 69 ALDVTDALDMQAFADFATHEFGKIDVIINNAGVMPLSPLAALKIAEWNQMLDVNVRGVLH 128 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 GIAAVLP+M+ Q G IIN+SS G VSPT AVY ATKFAVRAIS+GLR E +IR Sbjct: 129 GIAAVLPSMQAQGHGQIINISSIGGLAVSPTAAVYCATKFAVRAISDGLRQE---TDKIR 185 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 T++ PG+ ++EL DSI+ + V + P +IARA+ +AI +PD +SEI+V Sbjct: 186 VTVVCPGVVESELADSISDETAREAMKAFRKVALEPDAIARALVYAIEQPDGVDVSEIVV 245 Query: 241 RPT 243 RPT Sbjct: 246 RPT 248 >gb|EAI95436.1| unknown [environmental sequence] Length = 245 Score = 259 bits (663), Expect = 3e-68, Method: Compositional matrix adjust. Identities = 139/243 (57%), Positives = 178/243 (73%), Gaps = 2/243 (0%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M + KVVIITGASSG+GEATAK+LA GAK+VL ARRE+RL +V+EI+ GG+A Sbjct: 1 MSEVSGKVVIITGASSGLGEATAKMLADKGAKLVLGARREDRLKKLVDEIESSGGQATYQ 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 DV ++++ LAK A++ YGRIDV VNNAG+MP + L E++V EWDQMIDVNIKGV+Y Sbjct: 61 TVDVTKKDEVKALAKAAIDAYGRIDVLVNNAGLMPLAPLDEMKVDEWDQMIDVNIKGVMY 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 G+AAVL MREQ+SGHIINLSS AG+ V P VY ATKFAV+AISEG+R E S IR Sbjct: 121 GVAAVLGQMREQKSGHIINLSSVAGHVVFPGATVYCATKFAVKAISEGIRRE--SNGEIR 178 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 ST I+PG TEL D I+ + + +A+ + I +IARA+ FAI +P D I+E+++ Sbjct: 179 STNISPGAVATELTDHISHKDSKQMADDMYDDAIDADAIARAVTFAIEQPGDVDINEMII 238 Query: 241 RPT 243 RPT Sbjct: 239 RPT 241 >gb|EAF52543.1| unknown [environmental sequence] Length = 240 Score = 257 bits (657), Expect = 1e-67, Method: Compositional matrix adjust. Identities = 138/242 (57%), Positives = 177/242 (73%), Gaps = 2/242 (0%) Query: 1 MENIQNKVVIITGASSGIGEATAKLLAQNGAKVVLAARREERLNAIVNEIKQGGGEAVSV 60 M + KVVIITGASSG+GEATAK+LA GAK+VL ARRE+RL +V+EI+ GG+A Sbjct: 1 MSEVSGKVVIITGASSGLGEATAKMLADKGAKLVLGARREDRLKKLVDEIESSGGQATYQ 60 Query: 61 KADVISAEDMQKLAKFALNTYGRIDVWVNNAGVMPSSRLHELRVGEWDQMIDVNIKGVLY 120 DV ++++ LAK A++ YGRIDV VNNAG+MP + L E++V EWDQMIDVNIKGV+Y Sbjct: 61 TVDVTKKDEVKALAKAAIDAYGRIDVLVNNAGLMPLAPLDEMKVDEWDQMIDVNIKGVMY 120 Query: 121 GIAAVLPAMREQQSGHIINLSSTAGYQVSPTNAVYAATKFAVRAISEGLRLEESSASRIR 180 G+AAVL MREQ+SGHIINLSS AG+ V P VY ATKFAV+AISEG+R E S IR Sbjct: 121 GVAAVLGQMREQKSGHIINLSSVAGHVVFPGATVYCATKFAVKAISEGIRRE--SNGEIR 178 Query: 181 STLIAPGLTKTELFDSITSPEVQTIANQLSGVGISPYSIARAIAFAINEPDDTLISEIMV 240 ST I+PG TEL D I+ + + +A+ + I +IARA+ FAI +P D I+E+++ Sbjct: 179 STNISPGAVATELTDHISHKDSKQMADDMYDDAIDADAIARAVTFAIEQPGDVDINEMII 238 Query: 241 RP 242 RP Sbjct: 239 RP 240 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.130 0.350 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 302,998,417 Number of extensions: 10750286 Number of successful extensions: 52325 Number of sequences better than 1.0e-05: 8752 Number of HSP's gapped: 44516 Number of HSP's successfully gapped: 8770 Length of query: 246 Length of database: 757,237,578 Length adjustment: 122 Effective length of query: 124 Effective length of database: 425,995,500 Effective search space: 52823442000 Effective search space used: 52823442000 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 124 (52.4 bits)