BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0022.p (390 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00060113.1| COG4585: Signal transduction histidine kinase... 322 1e-86 ref|NP_244496.1| two-component sensor histidine kinase involved ... 315 1e-84 sp|P54663|DEGS_BACBR Sensor protein degS >gi|2119999|pir||I39834... 310 4e-83 ref|NP_693441.1| two-component sensor histidine kinase [Oceanoba... 308 2e-82 ref|NP_391430.1| two-component sensor histidine kinase [Bacillus... 294 2e-78 >ref|ZP_00060113.1| COG4585: Signal transduction histidine kinase [Clostridium thermocellum ATCC 27405] Length = 392 Score = 322 bits (824), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 166/378 (43%), Positives = 264/378 (69%), Gaps = 3/378 (0%) Query: 13 IDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQYHR 72 +D++IK TI+ + +SK ++F+I + AR+E V L KEL+ + T E ++ V+ LE+ + Sbjct: 12 LDKIIKKTIEAINNSKAELFDIAENARNECVRLEKELEELKRRTSEIIKSVETLEVALYE 71 Query: 73 SRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRSVEN 132 S+ RL VSR++ +YSE+++R AYE A +++EL + RERE Y RR+EL+MR++ Sbjct: 72 SKKRLMHVSRNYDKYSEEELREAYENADNIRVELAIKREREQYYIKRRNELEMRLKEAYK 131 Query: 133 SVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIAREIHD 192 +VE+A+++ SQ+ + L YL+G+L V+ +E K R+++G++II AQEEER+R+AREIHD Sbjct: 132 TVEKADNLISQIGISLSYLTGDLENVSLQIEDMKQRRLLGIRIIKAQEEERQRVAREIHD 191 Query: 193 GPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALDD 252 GPAQ ++N+VL+ EI ER LV + +DE+ LK VR +L ++RK+I++LRPM+LDD Sbjct: 192 GPAQSMSNIVLKAEICER-LVDSDPEKAKDELRTLKSVVRDTLRDVRKIIYDLRPMSLDD 250 Query: 253 LGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPSY 312 LGLIPTL++Y+ E++ I+ +F+TRG +L + V+RLVQEA++N KHA Sbjct: 251 LGLIPTLQRYIETCREESGIKITFKTRGTCEQLKPVVSLTVFRLVQEAVNNIKKHARADK 310 Query: 313 VLVEITYQAQLIKIVVKDNGLGFNVK--KTSEQANRESFGLVGMRERVELLEGRMEILSA 370 V + + + + +K+ + DNG+GF+ K++E+ + FGL+ MRERVELL+G+ EI SA Sbjct: 311 VTINLEFLEKELKLYIADNGVGFDFDSLKSNEEDINKGFGLISMRERVELLDGKFEIDSA 370 Query: 371 ENQGTTIVIHIPTNVEKG 388 +GT + I +P E+G Sbjct: 371 VGKGTRLNITVPLLPEEG 388 >ref|NP_244496.1| two-component sensor histidine kinase involved in degradative enzyme [Bacillus halodurans] pir||E84103 two-component sensor histidine kinase involved in degradative enzyme degS [imported] - Bacillus halodurans (strain C-125) dbj|BAB07348.1| two-component sensor histidine kinase involved in degradative enzyme [Bacillus halodurans] Length = 377 Score = 315 bits (807), Expect = 1e-84, Method: Compositional matrix adjust. Identities = 167/363 (46%), Positives = 248/363 (68%), Gaps = 4/363 (1%) Query: 12 AIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQYH 71 +D++I T+D + S+ QIFEI + +R+E V+L +ELQ V + E + + D+ E+ Sbjct: 6 TLDKIIDKTLDTVGKSREQIFEISEQSRNEYVSLEQELQEVRMKVAEIIDQSDRAEVHAR 65 Query: 72 RSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRSVE 131 +R RL EVS+ F RYS ++IR YE+A ELQ++L + ++ E LR RRD ++ R+ +++ Sbjct: 66 FARNRLAEVSKQFHRYSNEEIRKVYEQANELQVKLALLQQEEQQLRDRRDAIERRLLNLK 125 Query: 132 NSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIAREIH 191 +++ERAE + QM+VV +L+G+L QV +E A+ +Q GL+II AQEEER++++REIH Sbjct: 126 DTIERAEELVGQMTVVYNFLTGDLRQVGEALEDAREKQAFGLQIIQAQEEERRKLSREIH 185 Query: 192 DGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALD 251 DGPAQM+AN++LR+E+VER+ + E+ DL+ V+ SL E+R++I++LRPMALD Sbjct: 186 DGPAQMMANVLLRSELVERIYHDKGIDEALKEIRDLRKMVKSSLAEVRRIIYDLRPMALD 245 Query: 252 DLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPS 311 DLGLIPTL+KYV +EE T I F+ GK R +E A++RLVQEAL N KHA S Sbjct: 246 DLGLIPTLKKYVKTFEEHTGIFVDFKHIGKGERFPEHVEIALFRLVQEALQNTRKHAKAS 305 Query: 312 YVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRMEILSAE 371 +V V+I Q +V+KDNG GF+ + E SFGLVGM+ERV +L+G++ I + Sbjct: 306 HVHVKIEEQKTKFTVVIKDNGKGFDQTEKKEG----SFGLVGMKERVNMLKGQLVIRTKP 361 Query: 372 NQG 374 N G Sbjct: 362 NDG 364 >sp|P54663|DEGS_BACBR Sensor protein degS pir||I39834 protein kinase - Bacillus brevis gb|AAC41438.1| protein kinase Length = 386 Score = 310 bits (794), Expect = 4e-83, Method: Compositional matrix adjust. Identities = 166/363 (45%), Positives = 247/363 (68%), Gaps = 4/363 (1%) Query: 12 AIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQYH 71 +D++I T+D + S+ QIFEI + +R+E V+L +ELQ V + E + + D+ E+ Sbjct: 15 TLDKIIDKTLDTVGKSREQIFEISEQSRNEYVSLEQELQEVRMKVAEIIDQSDRAEVHAR 74 Query: 72 RSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRSVE 131 +R RL EVS+ F RYS ++IR YE+A ELQ++L + ++ E LR RRD ++ R+ +++ Sbjct: 75 FARNRLAEVSKQFHRYSNEEIRKVYEQANELQVKLALLQQEEQQLRDRRDAIERRLLNLK 134 Query: 132 NSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIAREIH 191 +++ERAE + QM+VV +L+G+L QV +E A+ +Q GL+II AQEEER++++REIH Sbjct: 135 DTIERAEELVGQMTVVYNFLTGDLRQVGEALEDAREKQAFGLQIIQAQEEERRKLSREIH 194 Query: 192 DGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRPMALD 251 DGPAQM+AN++LR+E+VER+ + E+ DL+ V+ SL E+R++I++LR MALD Sbjct: 195 DGPAQMMANVLLRSELVERIYHDKGIDEALKEIRDLRKMVKSSLAEVRRIIYDLRRMALD 254 Query: 252 DLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNAAKHAFPS 311 DLGLIPTL+KYV +EE T I F+ GK R +E A++RLVQEAL N KHA S Sbjct: 255 DLGLIPTLKKYVKTFEEHTGIFVDFKHIGKGERFPEHVEIALFRLVQEALQNTRKHAKAS 314 Query: 312 YVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGRMEILSAE 371 +V V+I Q +V+KDNG GF+ + E SFGLVGM+ERV +L+G++ I + Sbjct: 315 HVHVKIEEQKTKFTVVIKDNGKGFDQTEKKEG----SFGLVGMKERVNMLKGQLVIRTKP 370 Query: 372 NQG 374 N G Sbjct: 371 NDG 373 >ref|NP_693441.1| two-component sensor histidine kinase [Oceanobacillus iheyensis HTE831] dbj|BAC14476.1| two-component sensor histidine kinase involved in degradative enzyme [Oceanobacillus iheyensis HTE831] Length = 379 Score = 308 bits (788), Expect = 2e-82, Method: Compositional matrix adjust. Identities = 165/378 (43%), Positives = 254/378 (67%), Gaps = 4/378 (1%) Query: 5 SVEFQADAIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVD 64 +++ A+D V+ +DV+E+SK +IF I + AR E L +EL+ E+ +Q D Sbjct: 2 AIKITDKALDHVVNEMVDVVENSKDEIFNISEEARKEHEHLVRELKLTKEKVLRHIQNGD 61 Query: 65 KLELQYHRSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQ 124 +LE + SR RL EVS+ F +YSE +IR YE +Q +L + R+ E LR +RDEL+ Sbjct: 62 QLEERVRFSRKRLAEVSKYFEKYSEDEIREVYENTHLMQTKLAILRQEEKSLREKRDELE 121 Query: 125 MRVRSVENSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERK 184 R+ + +++ RAE++ +++V+L YL + QV ++E+AK +Q GLKII AQEEER+ Sbjct: 122 RRLIGLSHTINRAENLAGKIAVILNYLQDDFRQVNEMIENAKEKQEFGLKIIEAQEEERR 181 Query: 185 RIAREIHDGPAQMLANLVLRTEIVERMLVKQEFGLVQDEVLDLKGQVRYSLEEMRKVIFN 244 +++REIHDGPAQMLAN++LR+EI+ER + K +E+ ++ VR SL E+R++I++ Sbjct: 182 KLSREIHDGPAQMLANMLLRSEILERTIKKGTHDQAIEEIHSVRKMVRSSLYEVRRIIYD 241 Query: 245 LRPMALDDLGLIPTLRKYVHDYEEKTKIRTSFETRGKEHRLSSAMEAAVYRLVQEALSNA 304 LRPMALDDLGLIPT+RKYV E I +F G+E RL+ E A++RL+QE++ NA Sbjct: 242 LRPMALDDLGLIPTIRKYVESISEYHSITINFNVIGEEKRLNQKYEIALFRLMQESIQNA 301 Query: 305 AKHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGR 364 KH+ + + V++ + + + ++VKD G GF+ + S++ SFGL+GMRERVE+LEG Sbjct: 302 IKHSEAALIQVKLEIRRENLTLIVKDEGKGFDQNEKSDK----SFGLIGMRERVEMLEGE 357 Query: 365 MEILSAENQGTTIVIHIP 382 + I SA +GT+I+I +P Sbjct: 358 LNIKSAIGKGTSIIIKVP 375 >ref|NP_391430.1| two-component sensor histidine kinase [Bacillus subtilis] sp|P13799|DEGS_BACSU Sensor protein degS pir||RGBSDS degradative enzyme regulator / competence regulator degS - Bacillus subtilis gb|AAA22732.1| degS protein gb|AAA22734.1| transcriptional activator protein gb|AAC44937.1| histidine kinase emb|CAB15567.1| two-component sensor histidine kinase [Bacillus subtilis subsp. subtilis str. 168] Length = 385 Score = 294 bits (753), Expect = 2e-78, Method: Compositional matrix adjust. Identities = 169/381 (44%), Positives = 269/381 (70%), Gaps = 8/381 (2%) Query: 10 ADAIDRVIKNTIDVMESSKYQIFEILQVARDELVALTKELQRVMEETDETLQKVDKLELQ 69 + +D ++ + ++ SK ++F+I + +R + L +EL+++ ++ E ++ DKLE+Q Sbjct: 8 SKVLDSILMKMLKTVDGSKDEVFQIGEQSRQQYEQLVEELKQIKQQVYEVIELGDKLEVQ 67 Query: 70 YHRSRIRLTEVSRDFVRYSEKDIRVAYEKATELQLELMMTREREVYLRSRRDELQMRVRS 129 +R RL+EVSR+F R+SE++IR AYEKA +LQ+EL M ++RE LR RRD+L+ R+ Sbjct: 68 TRHARNRLSEVSRNFHRFSEEEIRNAYEKAHKLQVELTMIQQREKQLRERRDDLERRLLG 127 Query: 130 VENSVERAESIGSQMSVVLEYLSGELGQVTRIVESAKNRQMIGLKIILAQEEERKRIARE 189 ++ +ER+ES+ SQ++VVL YL+ +L +V ++ A+ +Q GL+II AQEEERKR++RE Sbjct: 128 LQEIIERSESLVSQITVVLNYLNQDLREVGLLLADAQAKQDFGLRIIEAQEEERKRVSRE 187 Query: 190 IHDGPAQMLANLVLRTEIVERMLVKQ--EFGLVQDEVLDLKGQVRYSLEEMRKVIFNLRP 247 IHDGPAQMLAN+++R+E++ER+ + E G E+ +L+ VR +L E+R++I++LRP Sbjct: 188 IHDGPAQMLANVMMRSELIERIFRDRGAEDGF--QEIKNLRQNVRNALYEVRRIIYDLRP 245 Query: 248 MALDDLGLIPTLRKYVHDYEE-KTKIRTSFETRG--KEHRLSSAMEAAVYRLVQEALSNA 304 MALDDLGLIPTLRKY++ EE K++ F+ G ++ RL+ E A++RL QEA+SNA Sbjct: 246 MALDDLGLIPTLRKYLYTTEEYNGKVKIHFQCIGETEDQRLAPQFEVALFRLAQEAVSNA 305 Query: 305 AKHAFPSYVLVEITYQAQLIKIVVKDNGLGFNVKKTSEQANRESFGLVGMRERVELLEGR 364 KH+ + V++ + +++KDNG GF++K+ E+ N+ SFGL+GM+ERV+LLEG Sbjct: 306 LKHSESEEITVKVEITKDFVILMIKDNGKGFDLKEAKEKKNK-SFGLLGMKERVDLLEGT 364 Query: 365 MEILSAENQGTTIVIHIPTNV 385 M I S GT I+I +P ++ Sbjct: 365 MTIDSKIGLGTFIMIKVPLSL 385 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.344 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 453,097,993 Number of extensions: 16600227 Number of successful extensions: 79453 Number of sequences better than 1.0e-05: 451 Number of HSP's gapped: 78819 Number of HSP's successfully gapped: 451 Length of query: 390 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 262 Effective length of database: 409,704,906 Effective search space: 107342685372 Effective search space used: 107342685372 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)