BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0019.p (393 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00182571.1| hypothetical protein Exigu209801 [Exiguobacte... 639 0.0 ref|NP_244166.1| S-adenosylmethionine synthetase [Bacillus halod... 630 e-179 ref|NP_390933.1| S-adenosylmethionine synthetase [Bacillus subti... 629 e-179 gb|AAB17066.1| S-adenosylmethionine synthetase 623 e-177 ref|NP_834465.1| S-adenosylmethionine synthetase [Bacillus cereu... 621 e-177 >ref|ZP_00182571.1| hypothetical protein Exigu209801 [Exiguobacterium sp. 255-15] Length = 402 Score = 639 bits (1647), Expect = 0.0, Method: Compositional matrix adjust. Identities = 307/392 (78%), Positives = 350/392 (89%), Gaps = 2/392 (0%) Query: 1 LFTSESVTEGHPDKICDQISDAVLDAFLANDPNARVACEVAVATGLVLVIGEISTKSEYV 60 LFTSESVTEGHPDKICDQISD++LDA LA DPNARVA E +V TGLVLV GEI+T S YV Sbjct: 8 LFTSESVTEGHPDKICDQISDSILDAILAADPNARVAAETSVTTGLVLVAGEITT-STYV 66 Query: 61 DIPAIVRNTLKEIGYTRAKYGFDYNTCAVLTSLNEQSADIAQGVNAALENRDPAQVAEET 120 DIP +VR T++EIGYTRAKYGFD +TCAVLTS++EQSADIA GV+ ALE R+ EE Sbjct: 67 DIPKVVRETIREIGYTRAKYGFDADTCAVLTSIDEQSADIALGVDQALEAREGNMSDEEI 126 Query: 121 ANIGAGDQGLMFGFATNETPELMPLPIALSHRIARRLSEVRKDGTLNYLRPDGKTQVTIE 180 IGAGDQGLMFG+AT ETPELMPLPI+LSH++ARRL+EVRK+G L+YLRPDGKTQVT+E Sbjct: 127 DAIGAGDQGLMFGYATKETPELMPLPISLSHKLARRLAEVRKNGQLDYLRPDGKTQVTVE 186 Query: 181 Y-DNERPVRVDTIVVSTQHAEEISLEQIQADIKEYVILPVVPSELLDENTKYFINPTGRF 239 Y +N PVRVDTIV+STQHAEE++LEQIQAD+KE+VI PV+P+E +D +TK+FINPTGRF Sbjct: 187 YNENNEPVRVDTIVISTQHAEEVTLEQIQADLKEHVIAPVIPTEYIDADTKFFINPTGRF 246 Query: 240 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL 299 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL Sbjct: 247 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL 306 Query: 300 ADKCEIQLAYAIGVANPVSINVDTYGTGKISEEKLAELISSNFDLRPAGIISMLDLRKPR 359 ADK E+QLAYAIGVA+PVSI VDT+GTGK+ E +L EL++ NFDLRPAGII+MLDLR+P Sbjct: 307 ADKAEVQLAYAIGVAHPVSIAVDTFGTGKLPEAQLVELVAENFDLRPAGIINMLDLRRPI 366 Query: 360 YRQTAAYGHFGRTDVDLPWERVDKAEMLRGQA 391 YRQTAAYGHFGRTD++LPWE+ DKA L QA Sbjct: 367 YRQTAAYGHFGRTDIELPWEQTDKAATLEEQA 398 >ref|NP_244166.1| S-adenosylmethionine synthetase [Bacillus halodurans] sp|Q9K7Q9|METK_BACHD S-adenosylmethionine synthetase (Methionine adenosyltransferase) (AdoMet synthetase) (MAT) pir||D84062 S-adenosylmethionine synthetase metK [imported] - Bacillus halodurans (strain C-125) dbj|BAB07019.1| S-adenosylmethionine synthetase [Bacillus halodurans] Length = 399 Score = 630 bits (1626), Expect = e-179, Method: Compositional matrix adjust. Identities = 300/392 (76%), Positives = 343/392 (87%), Gaps = 2/392 (0%) Query: 1 LFTSESVTEGHPDKICDQISDAVLDAFLANDPNARVACEVAVATGLVLVIGEISTKSEYV 60 LFTSESVTEGHPDKICDQISD++LD L DPNARVACE +V TGLVLV GEI+T S YV Sbjct: 9 LFTSESVTEGHPDKICDQISDSILDEILKEDPNARVACETSVTTGLVLVAGEITT-STYV 67 Query: 61 DIPAIVRNTLKEIGYTRAKYGFDYNTCAVLTSLNEQSADIAQGVNAALENRDPAQVAEET 120 DIP +VR+T++ IGYTRAKYGFD TCAVLTS++EQS DIAQGVN ALE R+ E Sbjct: 68 DIPKVVRDTIRNIGYTRAKYGFDSETCAVLTSIDEQSPDIAQGVNQALEAREGQMTDAEI 127 Query: 121 ANIGAGDQGLMFGFATNETPELMPLPIALSHRIARRLSEVRKDGTLNYLRPDGKTQVTIE 180 IGAGDQGLMFG+A NETPELMPLPI+LSH++ARRLSE RK L YLRPDGKTQVT+E Sbjct: 128 EAIGAGDQGLMFGYANNETPELMPLPISLSHKLARRLSEARKGEILPYLRPDGKTQVTVE 187 Query: 181 YD-NERPVRVDTIVVSTQHAEEISLEQIQADIKEYVILPVVPSELLDENTKYFINPTGRF 239 YD N++ VR+DTIV+STQH E++LEQI++D+K++VI VVP EL+DE TKYFINPTGRF Sbjct: 188 YDENDQSVRIDTIVISTQHHPEVTLEQIESDLKQHVIRSVVPEELIDEETKYFINPTGRF 247 Query: 240 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL 299 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRS AYAARYVAKN+VAAGL Sbjct: 248 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSGAYAARYVAKNIVAAGL 307 Query: 300 ADKCEIQLAYAIGVANPVSINVDTYGTGKISEEKLAELISSNFDLRPAGIISMLDLRKPR 359 ADKCE+QLAYAIGVA PVSI++DT+GTG++SE +L EL+ +FDLRPAGII MLDLR+P Sbjct: 308 ADKCEVQLAYAIGVAKPVSISIDTFGTGQVSEARLVELVREHFDLRPAGIIKMLDLRRPI 367 Query: 360 YRQTAAYGHFGRTDVDLPWERVDKAEMLRGQA 391 Y+QTAAYGHFGRTDV+LPWE+ DKAE+LR QA Sbjct: 368 YKQTAAYGHFGRTDVELPWEQTDKAEILRQQA 399 >ref|NP_390933.1| S-adenosylmethionine synthetase [Bacillus subtilis] sp|P54419|METK_BACSU S-adenosylmethionine synthetase (Methionine adenosyltransferase) (AdoMet synthetase) (MAT) pir||D69657 methionine adenosyltransferase (EC 2.5.1.6) - Bacillus subtilis gb|AAC00242.1| SAM synthase [Bacillus subtilis] emb|CAB15033.1| S-adenosylmethionine synthetase [Bacillus subtilis subsp. subtilis str. 168] Length = 400 Score = 629 bits (1622), Expect = e-179, Method: Compositional matrix adjust. Identities = 304/392 (77%), Positives = 340/392 (86%), Gaps = 2/392 (0%) Query: 1 LFTSESVTEGHPDKICDQISDAVLDAFLANDPNARVACEVAVATGLVLVIGEISTKSEYV 60 LFTSESVTEGHPDKICDQISD++LD L DPNARVACE +V TGLVLV GEI+T S YV Sbjct: 7 LFTSESVTEGHPDKICDQISDSILDEILKKDPNARVACETSVTTGLVLVSGEITT-STYV 65 Query: 61 DIPAIVRNTLKEIGYTRAKYGFDYNTCAVLTSLNEQSADIAQGVNAALENRDPAQVAEET 120 DIP VR T+KEIGYTRAKYGFD TCAVLTS++EQSADIA GV+ ALE R+ EE Sbjct: 66 DIPKTVRQTIKEIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGTMSDEEI 125 Query: 121 ANIGAGDQGLMFGFATNETPELMPLPIALSHRIARRLSEVRKDGTLNYLRPDGKTQVTIE 180 IGAGDQGLMFG+A NET ELMPLPI+L+H++ARRLSEVRK+ L YLRPDGKTQVT+E Sbjct: 126 EAIGAGDQGLMFGYACNETKELMPLPISLAHKLARRLSEVRKEDILPYLRPDGKTQVTVE 185 Query: 181 YD-NERPVRVDTIVVSTQHAEEISLEQIQADIKEYVILPVVPSELLDENTKYFINPTGRF 239 YD N +PVR+D IV+STQH EI+LEQIQ +IKE+VI PVVP EL+DE TKYFINPTGRF Sbjct: 186 YDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFINPTGRF 245 Query: 240 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL 299 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKD TKVDRSAAYAARYVAKN+VAA L Sbjct: 246 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAEL 305 Query: 300 ADKCEIQLAYAIGVANPVSINVDTYGTGKISEEKLAELISSNFDLRPAGIISMLDLRKPR 359 AD CE+QLAYAIGVA PVSI+++T+G+GK SEEKL E++ +NFDLRPAGII MLDLR+P Sbjct: 306 ADSCEVQLAYAIGVAQPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLDLRRPI 365 Query: 360 YRQTAAYGHFGRTDVDLPWERVDKAEMLRGQA 391 Y+QTAAYGHFGR DVDLPWER DKAE LR +A Sbjct: 366 YKQTAAYGHFGRHDVDLPWERTDKAEQLRKEA 397 >gb|AAB17066.1| S-adenosylmethionine synthetase Length = 400 Score = 623 bits (1607), Expect = e-177, Method: Compositional matrix adjust. Identities = 302/392 (77%), Positives = 338/392 (86%), Gaps = 2/392 (0%) Query: 1 LFTSESVTEGHPDKICDQISDAVLDAFLANDPNARVACEVAVATGLVLVIGEISTKSEYV 60 LFTSESVTEGHPDKICDQI D++LD L DPNARVACE +V TGLVLV GEI+T S YV Sbjct: 7 LFTSESVTEGHPDKICDQIYDSILDEILKKDPNARVACETSVTTGLVLVSGEITT-STYV 65 Query: 61 DIPAIVRNTLKEIGYTRAKYGFDYNTCAVLTSLNEQSADIAQGVNAALENRDPAQVAEET 120 DIP VR T+KEIGYTRAKYGFD TCAVLTS++EQSADIA GV+ ALE R+ EE Sbjct: 66 DIPKTVRQTIKEIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDPALEAREGTMSDEEI 125 Query: 121 ANIGAGDQGLMFGFATNETPELMPLPIALSHRIARRLSEVRKDGTLNYLRPDGKTQVTIE 180 IGAGDQGLMFG+ NET ELMPLPI+L+H++ARRLSEVRK+ L YLRPDGKTQVT+E Sbjct: 126 EAIGAGDQGLMFGYVCNETKELMPLPISLAHKLARRLSEVRKEDILPYLRPDGKTQVTVE 185 Query: 181 YD-NERPVRVDTIVVSTQHAEEISLEQIQADIKEYVILPVVPSELLDENTKYFINPTGRF 239 YD N +PVR+D IV+STQH EI+LEQIQ +IKE+VI PVVP EL+DE TKYFINPTGRF Sbjct: 186 YDENNKPVRIDAIVISTQHHPEITLEQIQRNIKEHVINPVVPEELIDEETKYFINPTGRF 245 Query: 240 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL 299 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKD TKVDRSAAYAARYVAKN+VAA L Sbjct: 246 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAEL 305 Query: 300 ADKCEIQLAYAIGVANPVSINVDTYGTGKISEEKLAELISSNFDLRPAGIISMLDLRKPR 359 AD CE+QLAYAIGVA PVSI+++T+G+GK SEEKL E++ +NFDLRPAGII MLDLR+P Sbjct: 306 ADSCEVQLAYAIGVAQPVSISINTFGSGKASEEKLIEVVRNNFDLRPAGIIKMLDLRRPI 365 Query: 360 YRQTAAYGHFGRTDVDLPWERVDKAEMLRGQA 391 Y+QTAAYGHFGR DVDLPWER DKAE LR +A Sbjct: 366 YKQTAAYGHFGRHDVDLPWERTDKAEQLRKEA 397 >ref|NP_834465.1| S-adenosylmethionine synthetase [Bacillus cereus ATCC 14579] sp|Q816Q8|METK_BACCR S-adenosylmethionine synthetase (Methionine adenosyltransferase) (AdoMet synthetase) (MAT) gb|AAP11666.1| S-adenosylmethionine synthetase [Bacillus cereus ATCC 14579] Length = 399 Score = 621 bits (1602), Expect = e-177, Method: Compositional matrix adjust. Identities = 301/394 (76%), Positives = 341/394 (86%), Gaps = 2/394 (0%) Query: 1 LFTSESVTEGHPDKICDQISDAVLDAFLANDPNARVACEVAVATGLVLVIGEISTKSEYV 60 LFTSESVTEGHPDKICDQISD++LDA L+ D NARVACE V TGLVLV GEI+T S YV Sbjct: 7 LFTSESVTEGHPDKICDQISDSILDAILSKDANARVACETTVTTGLVLVAGEITT-STYV 65 Query: 61 DIPAIVRNTLKEIGYTRAKYGFDYNTCAVLTSLNEQSADIAQGVNAALENRDPAQVAEET 120 DIP IVR T++ IGYTRAKYGFD TCAVLTS++EQSADIA GV+ ALE R+ E Sbjct: 66 DIPKIVRETIQGIGYTRAKYGFDAETCAVLTSIDEQSADIAMGVDQALEAREGQMTDAEI 125 Query: 121 ANIGAGDQGLMFGFATNETPELMPLPIALSHRIARRLSEVRKDGTLNYLRPDGKTQVTIE 180 IGAGDQGLMFGFA NET ELMPLPI+L+H++ARRL+EVRKD TL+YLRPDGKTQVT+E Sbjct: 126 EAIGAGDQGLMFGFACNETQELMPLPISLAHKLARRLTEVRKDDTLSYLRPDGKTQVTVE 185 Query: 181 YD-NERPVRVDTIVVSTQHAEEISLEQIQADIKEYVILPVVPSELLDENTKYFINPTGRF 239 YD N +PVRVDTIV+STQH +++ E+I D+KE+VI VVP+EL+D TK+FINPTGRF Sbjct: 186 YDENGKPVRVDTIVISTQHHPDVTWEEIDRDLKEHVIKAVVPAELMDGETKFFINPTGRF 245 Query: 240 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSAAYAARYVAKNLVAAGL 299 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKD TKVDRSAAYAARYVAKN+VAAGL Sbjct: 246 VIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFSGKDATKVDRSAAYAARYVAKNIVAAGL 305 Query: 300 ADKCEIQLAYAIGVANPVSINVDTYGTGKISEEKLAELISSNFDLRPAGIISMLDLRKPR 359 A+K E+QLAYAIGVA PVSI+VDT+GTGK+SE+ L EL+ +NFDLRPAGII MLDLR+P Sbjct: 306 AEKAEVQLAYAIGVAQPVSISVDTFGTGKVSEDVLVELVRNNFDLRPAGIIKMLDLRRPI 365 Query: 360 YRQTAAYGHFGRTDVDLPWERVDKAEMLRGQAGL 393 Y+QTAAYGHFGRTDVDL WER DKA L+ QAGL Sbjct: 366 YKQTAAYGHFGRTDVDLSWERTDKAAALKEQAGL 399 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.135 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 584,883,397 Number of extensions: 25052414 Number of successful extensions: 67099 Number of sequences better than 1.0e-05: 1153 Number of HSP's gapped: 64820 Number of HSP's successfully gapped: 1157 Length of query: 393 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 265 Effective length of database: 409,704,906 Effective search space: 108571800090 Effective search space used: 108571800090 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 127 (53.5 bits)