BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0327.0013.p (1306 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||I39917 hypothetical protein - Bacillus sp >gi|460687|dbj|BA... 102 6e-20 gb|AAG44799.1| amylopullulanase [Geobacillus stearothermophilus] 102 8e-20 gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurig... 99 7e-19 ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter ten... 99 9e-19 sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pu... 98 1e-18 >pir||I39917 hypothetical protein - Bacillus sp dbj|BAA05832.1| ORF [Bacillus sp.] Length = 2032 Score = 102 bits (255), Expect = 6e-20, Method: Compositional matrix adjust. Identities = 71/212 (33%), Positives = 109/212 (51%), Gaps = 26/212 (12%) Query: 1092 YTVMKLRRGYTDITNHPWARNILNGLYSKGIMENLRFDQFGADDTTTRGEFATLLVKGLN 1151 Y V++ ++DI H WA+ + L +K +++ + + ++ TR +FA LLV+ L Sbjct: 1836 YVVVEGTHTFSDIEKH-WAKGYIETLAAKQLVKGMTETAYRPNEQMTRAQFAVLLVRALA 1894 Query: 1152 IPLNYNNNQQTFFDIVPEAVSTTWDFKHIETAA--RAGIVTGLSDGFFGPDQPLTREQGA 1209 +P +T+ + T W K+ E AA + GI+ G + F P++P+TR Q A Sbjct: 1895 LP------HETYDGRFADVKGTEWFNKNGELAAAVKLGIIQGKTANTFAPNEPITRVQAA 1948 Query: 1210 VMIARALKLKM-----ATNDAKLQATLAKSFVDTGSMDFYSKPAIDAVSKAKIMEGSAVT 1264 VMI RALKL ATND +AT F D + ++K AI+AV +A IM+G Sbjct: 1949 VMIERALKLSFVGYDEATNDKTKKAT---DFRDAKQLPTWAKQAIEAVYQAGIMQG---- 2001 Query: 1265 ITGQTKPVYNFNPKGKMTRAEAGKISVALLKK 1296 + +F+P G MTRAE K+ L K Sbjct: 2002 -----RDNGSFDPTGHMTRAEMAKVLAEFLGK 2028 >gb|AAG44799.1| amylopullulanase [Geobacillus stearothermophilus] Length = 2018 Score = 102 bits (253), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 67/210 (31%), Positives = 104/210 (49%), Gaps = 20/210 (9%) Query: 1092 YTVMKLRRGYTDITNHPWARNILNGLYSKGIMENLRFDQFGADDTTTRGEFATLLVKGLN 1151 Y V++ + DI H WA+ ++ L +K ++ + + ++ TR +FA LLV+ L Sbjct: 1822 YVVVEGTTTFADIVQH-WAKPYIDSLAAKQLVRGVTETAYRPNEPMTRAQFAVLLVRALA 1880 Query: 1152 IPLNYNNNQQTFFDIVPEAVSTTWDFKHIETAA--RAGIVTGLSDGFFGPDQPLTREQGA 1209 +P +T+ + T W +H E AA + G++ G + F P++P+TR Q A Sbjct: 1881 LP------SETYDGRFADVKGTEWFNQHGELAAAVKYGVIQGKTPSTFAPNEPITRAQAA 1934 Query: 1210 VMIARALKLKMATND--AKLQATLAKSFVDTGSMDFYSKPAIDAVSKAKIMEGSAVTITG 1267 VMI RAL L D AK Q F D + +SK AI+A+ +A I++G Sbjct: 1935 VMIERALNLSFVNFDKAAKDQTKKLADFRDANQLPAWSKQAIEAIYQAGIVQG------- 1987 Query: 1268 QTKPVYNFNPKGKMTRAEAGKISVALLKKS 1297 P F P G+MTRAE K+ L K+ Sbjct: 1988 --HPDGTFAPAGRMTRAEMAKVLAEFLAKA 2015 >gb|AAB00841.1| pullulanase [Thermoanaerobacterium thermosulfurigenes] Length = 1861 Score = 99.0 bits (245), Expect = 7e-19, Method: Compositional matrix adjust. Identities = 72/246 (29%), Positives = 121/246 (49%), Gaps = 23/246 (9%) Query: 1057 ITVFRYTDS-GQWENIGGEVDTKKHTISVPFDQFGYYTVMKLRRGYTDITNHPWARNILN 1115 + V+ Y + QWE +GG+VD TI+ F Y + + + DI ++ WA++++ Sbjct: 1637 VAVYYYNPTTNQWEYVGGKVDASSGTITFNATHFSQYAAFEYDKTFNDIKDN-WAKDVIE 1695 Query: 1116 GLYSKGIMENLRFDQFGADDTTTRGEFATLLVKGLNIPLNYNNNQQTFFDIVPEAVSTTW 1175 L S+ I+E + Q+ + T TR EF ++++ LNI ++T+ + S W Sbjct: 1696 VLASRHIVEGMTDTQYEPNKTVTRAEFTAMILRLLNIK------EETYSGEFSDVKSGDW 1749 Query: 1176 DFKHIETAARAGIVTGLSDGFFG-PDQPLTREQGAVMIARALKLKMATNDAKLQATLAKS 1234 IE A +AGI+ G DG P+ +TRE+ + RA ++ + + AT + Sbjct: 1750 YANAIEAAYKAGIIEG--DGKNARPNDSITREEMTAIAMRAYEMLTQYKEENIGAT---T 1804 Query: 1235 FVDTGSMDFYSKPAIDAVSKAKIMEGSAVTITGQTKPVYNFNPKGKMTRAEAGKISVALL 1294 F D S+ +++ + +K I+ G +P F PKG TRAEA I LL Sbjct: 1805 FSDDKSISDWARNVVANAAKLGIVNG---------EPNNVFAPKGNATRAEAAAIIYGLL 1855 Query: 1295 KKSTSI 1300 +KS +I Sbjct: 1856 EKSGNI 1861 >ref|NP_623945.1| S-layer homology domain [Thermoanaerobacter tengcongensis] gb|AAM25549.1| S-layer homology domain [Thermoanaerobacter tengcongensis] Length = 1321 Score = 98.6 bits (244), Expect = 9e-19, Method: Compositional matrix adjust. Identities = 76/289 (26%), Positives = 125/289 (43%), Gaps = 23/289 (7%) Query: 1009 KPATNGIAPYSIEGNFTQIEAERKIVPSQRGKLTLTFNSSIVDEVGSTITVFRYTDSGQW 1068 KP N P S + T E KI + LTF ++ + +G+W Sbjct: 1051 KPEANAYKPVSNAYDITIKAGESKI--KLESPVKLTFEVKDAKDIRKVGVYYLNEATGKW 1108 Query: 1069 ENIGGEVDTKKHTISVPFDQFGYYTVMKLRRGYTDITNHPWARNILNGLYSKGIMENLRF 1128 E +GG+VD +T++ F Y + + + D+ WA +++N L SK I++ + Sbjct: 1109 EYVGGKVDKNTNTVTAEVKHFSTYGAFEYDKQFKDVPKDFWAYDVINVLASKHIIKGMDD 1168 Query: 1129 DQFGADDTTTRGEFATLLVKGLNIPLNYNNNQQTFFDIVPEAVSTTWDFKHIETAARAGI 1188 D F + TR EFA L+++ L IP ++ + + + W IE A RAGI Sbjct: 1169 DNFAPNAKITRAEFAALMIRALGIP------EEPYKGEFEDVKAGAWYANAIEAAYRAGI 1222 Query: 1189 VTGLSDG-FFGPDQPLTREQGAVMIARALKLKMATNDAKLQATLAKSFVDTGSMDFYSKP 1247 + G DG PD P+TRE+ A +I R + + T+ F D S+ +++ Sbjct: 1223 MMG--DGKKMRPDDPITREEMAAVIMRVYAKMTGYKEENIGNTV---FKDNDSISEWARN 1277 Query: 1248 AIDAVSKAKIMEGSAVTITGQTKPVYNFNPKGKMTRAEAGKISVALLKK 1296 + +K I++G F K TRAE + +L+K Sbjct: 1278 VVANAAKLGIVKGYEDN---------TFRAKNNATRAEGVTLLYRILEK 1317 >sp|P38536|APU_THETU Amylopullulanase precursor (Alpha-amylase/pullulanase) (Pullulanase type II) [Includes: Alpha-amylase (1,4-alpha-D-glucan glucanohydrolase); Pullulanase (1,4-alpha-D-glucan glucanohydrolase) (Alpha-dextrin endo-1,6-alpha-glucosidase)] Length = 1861 Score = 98.2 bits (243), Expect = 1e-18, Method: Compositional matrix adjust. Identities = 72/246 (29%), Positives = 120/246 (48%), Gaps = 23/246 (9%) Query: 1057 ITVFRYTDS-GQWENIGGEVDTKKHTISVPFDQFGYYTVMKLRRGYTDITNHPWARNILN 1115 + V+ Y + QWE +GG+VD TI+ F Y + + + DI ++ WA++++ Sbjct: 1637 VAVYYYNPTTNQWEYVGGKVDASSGTITFNATHFSQYAAFEYDKTFNDIKDN-WAKDVIE 1695 Query: 1116 GLYSKGIMENLRFDQFGADDTTTRGEFATLLVKGLNIPLNYNNNQQTFFDIVPEAVSTTW 1175 L S+ I+E + Q+ + T TR EF ++++ LNI +T+ + S W Sbjct: 1696 VLASRHIVEGMTDTQYEPNKTVTRAEFTAMILRLLNIK------DETYSGEFSDVKSGDW 1749 Query: 1176 DFKHIETAARAGIVTGLSDGFFG-PDQPLTREQGAVMIARALKLKMATNDAKLQATLAKS 1234 IE A +AGI+ G DG P+ +TRE+ + RA ++ + + AT + Sbjct: 1750 YANAIEAAYKAGIIEG--DGKNARPNDSITREEMTAIAMRAYEMLTQYKEENIGAT---T 1804 Query: 1235 FVDTGSMDFYSKPAIDAVSKAKIMEGSAVTITGQTKPVYNFNPKGKMTRAEAGKISVALL 1294 F D S+ +++ + +K I+ G +P F PKG TRAEA I LL Sbjct: 1805 FSDDKSISDWARNVVANAAKLGIVNG---------EPNNVFAPKGNATRAEAAAIIYGLL 1855 Query: 1295 KKSTSI 1300 +KS +I Sbjct: 1856 EKSGNI 1861 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.370 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 1,889,434,639 Number of extensions: 81877217 Number of successful extensions: 171269 Number of sequences better than 1.0e-05: 66 Number of HSP's gapped: 171227 Number of HSP's successfully gapped: 68 Length of query: 1306 Length of database: 757,237,578 Length adjustment: 138 Effective length of query: 1168 Effective length of database: 382,553,916 Effective search space: 446822973888 Effective search space used: 446822973888 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 132 (55.5 bits)