BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0311.0023.p (196 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|EAK46938.1| unknown [environmental sequence] 132 5e-30 ref|ZP_00025395.1| COG1309: Transcriptional regulator [Ralstonia... 121 1e-26 ref|NP_626731.1| putative tetR-family transcriptional regulator ... 117 1e-25 gb|EAJ06300.1| unknown [environmental sequence] 115 5e-25 ref|NP_625388.1| putative tetR-family regulatory protein [Strept... 114 1e-24 >gb|EAK46938.1| unknown [environmental sequence] Length = 186 Score = 132 bits (332), Expect = 5e-30, Method: Compositional matrix adjust. Identities = 69/186 (37%), Positives = 101/186 (54%), Gaps = 1/186 (0%) Query: 1 MVRPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALR 60 M RPREFDE L AA FW GY ATS+ DL MGI S+++AFGDK+ LFE AL Sbjct: 1 MARPREFDENAVLDAAAHQFWVYGYTATSVRDLAQGMGITGASLFNAFGDKRSLFERALA 60 Query: 61 MYTKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAK 120 Y + + + A +AFF +VE R+ KGC +N+ +E++PHDA Sbjct: 61 HYIERSFGDRVSRFERSLPPLEAIKAFFREIVERSVRDR-DRKGCLLVNSALEVAPHDAD 119 Query: 121 FEILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTVMMKCRPERN 180 F+ + + + F+ + G Q+GE+ S A+ LA L+ +G+ V+ + RPER Sbjct: 120 FQAMIANVLVEVEAFFRRCVNAGQQTGEISSAQPAEDLARLLLGVHLGIRVLARTRPERA 179 Query: 181 FMDQAV 186 ++ V Sbjct: 180 LLEGVV 185 >ref|ZP_00025395.1| COG1309: Transcriptional regulator [Ralstonia metallidurans] Length = 200 Score = 121 bits (303), Expect = 1e-26, Method: Compositional matrix adjust. Identities = 67/191 (35%), Positives = 98/191 (51%), Gaps = 1/191 (0%) Query: 1 MVRPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALR 60 M RPR+FD + AL AA FW KGY ATS +LT MG+ +PS+Y+AFGDK+ LF AL Sbjct: 1 MARPRQFDAQTALSAAQDAFWAKGYNATSTRELTQAMGLTQPSLYNAFGDKRTLFLQALE 60 Query: 61 MYTKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAK 120 Y A L A AFF +E + ++GC INT ++ S D + Sbjct: 61 DYLNHTLRERIARLEGTLPPAQALEAFFAESIERGV-SDPQHRGCMLINTALDASADDPE 119 Query: 121 FEILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTVMMKCRPERN 180 + E L F+ T+ G G +D A+ ++ +L+ IGL VM + P+R Sbjct: 120 LAAIVAEELTTLGHFFERTIRAGQADGTVDPTLAARDVSATLLSVQIGLRVMSRVMPDRA 179 Query: 181 FMDQAVYSMLS 191 ++ +V +L+ Sbjct: 180 LLEASVRPVLA 190 >ref|NP_626731.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] emb|CAB69777.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] Length = 194 Score = 117 bits (293), Expect = 1e-25, Method: Compositional matrix adjust. Identities = 62/178 (34%), Positives = 90/178 (50%) Query: 1 MVRPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALR 60 M R +EFD + AL AA+++FW +GYEATS+SDL +G+ R SIY+ FG+K+ L+ AL Sbjct: 1 MARTKEFDPDAALRAALELFWRRGYEATSMSDLVEHLGVGRASIYATFGNKRELYLKALA 60 Query: 61 MYTKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAK 120 Y + + A L + RA E ++ GCF NT EL+ HDA Sbjct: 61 RYEQGLLPDLLADLSRPGPALPGVRALVRRYAAEASADDSRLHGCFVTNTAAELAAHDAV 120 Query: 121 FEILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTVMMKCRPE 178 + L + L R G+L +G D LA L++ L G+ V+ K P+ Sbjct: 121 AARRVERNWDQLETVLHSALARARAEGDLPAGRDPLALARMLLVLLQGMRVVGKASPD 178 >gb|EAJ06300.1| unknown [environmental sequence] Length = 200 Score = 115 bits (288), Expect = 5e-25, Method: Compositional matrix adjust. Identities = 67/173 (38%), Positives = 92/173 (53%), Gaps = 10/173 (5%) Query: 3 RPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALRMY 62 RPREFD + AL A+ +FW KGYE TSL DLT MGI RPSIYS FG+K+ LF A+ +Y Sbjct: 8 RPREFDIDDALQKALNVFWNKGYEGTSLPDLTKAMGINRPSIYSTFGNKEQLFLKAVELY 67 Query: 63 TKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSP--HDAK 120 SL++ + K A R EY + KGC + + + S K Sbjct: 68 ESQVGGFFYPSLNEPTAYKVAERILLG--AAAEYGNPHNPKGCMMVQSALACSDSGQSVK 125 Query: 121 FEILTREHQMY--LAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTV 171 E++ R Y L V FQE + SG+LD+ TD + LA ++ + GL++ Sbjct: 126 NELIARRQMGYSRLVVRFQE----AVTSGDLDNDTDPEALAAFILTVVQGLSI 174 >ref|NP_625388.1| putative tetR-family regulatory protein [Streptomyces coelicolor A3(2)] emb|CAB95981.1| putative tetR-family regulatory protein [Streptomyces coelicolor A3(2)] Length = 192 Score = 114 bits (285), Expect = 1e-24, Method: Compositional matrix adjust. Identities = 69/192 (35%), Positives = 96/192 (50%), Gaps = 2/192 (1%) Query: 1 MVRPREFDEEKALHAAMQIFWEKGYEATSLSDLTATMGIQRPSIYSAFGDKKGLFEAALR 60 M R REFD E A+ AM++FW +GYEATS+ DLT +GI + S+Y+AFGDK GL+ AAL Sbjct: 1 MARTREFDTEAAVSRAMELFWTRGYEATSVRDLTGHLGIGQGSLYAAFGDKDGLYRAALE 60 Query: 61 MYTKSHAASVRASLHKKASVKAAFRAFFEHLVEEEYREEGSNKGCFCINTMVELSPHDAK 120 Y + AA+ SL + ++A R + + G +GC +N E P DA Sbjct: 61 HYRTTLAAAALRSLEEGTDARSAIRTMLVERIRIAVDDNG--RGCLAVNAACERLPEDAA 118 Query: 121 FEILTREHQMYLAVIFQETLERGIQSGELDSGTDAKGLAHSLVIALIGLTVMMKCRPERN 180 R+ Q E L + GE+ D LA LV L GL V K P+ Sbjct: 119 TRRTVRDMQDAGRDALTEVLRVAAERGEIAKRHDPHTLAAFLVTFLNGLLVSSKITPDVR 178 Query: 181 FMDQAVYSMLSS 192 ++ V L++ Sbjct: 179 ALEPLVEVALAA 190 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.132 0.377 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 256,506,101 Number of extensions: 8857859 Number of successful extensions: 19800 Number of sequences better than 1.0e-05: 122 Number of HSP's gapped: 19806 Number of HSP's successfully gapped: 122 Length of query: 196 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 77 Effective length of database: 434,140,797 Effective search space: 33428841369 Effective search space used: 33428841369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 122 (51.6 bits)