BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0065.p (410 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00061359.1| COG4962: Flp pilus assembly protein, ATPase C... 490 e-137 gb|EAI69822.1| unknown [environmental sequence] 353 3e-96 ref|ZP_00029043.1| COG4962: Flp pilus assembly protein, ATPase C... 347 2e-94 ref|NP_936652.1| TadA protein [Vibrio vulnificus YJ016] >gi|3720... 331 2e-89 ref|NP_762074.1| Flp pilus assembly protein [Vibrio vulnificus C... 331 2e-89 >ref|ZP_00061359.1| COG4962: Flp pilus assembly protein, ATPase CpaF [Clostridium thermocellum ATCC 27405] Length = 412 Score = 490 bits (1261), Expect = e-137, Method: Compositional matrix adjust. Identities = 243/405 (60%), Positives = 301/405 (74%), Gaps = 1/405 (0%) Query: 3 EEMFRLLRSDIRSGLDLTSAVGNRELTAYIEQTVLARDSLRHLTAQEKHTLVKKLFDSFR 62 E++ +R I LDL + E+ I V R +L+ EK + +F+S R Sbjct: 7 EKLIAQIRKHISENLDLRKDFSDEEIKDIITNVVFERSRDYYLSVGEKKEIADAIFNSMR 66 Query: 63 XXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSGVNRV 122 LQPL+D+ +ITEIMIN D IF+E +G + +L + FES +LED+IQ++VS VNR Sbjct: 67 RLDVLQPLIDDKSITEIMINGPDSIFIERDGRVSKLNVKFESRRKLEDVIQTIVSRVNRT 126 Query: 123 VNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSMEAAEL 182 VN++SPIVDARL DGSRVN+VLPP+AL GP +TIRKFPE PMT+ +LI+ +++ E AE+ Sbjct: 127 VNEASPIVDARLPDGSRVNVVLPPIALNGPVVTIRKFPEKPMTIEQLIKYGSITEEVAEV 186 Query: 183 LQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLVSLET 242 L+ LV AKYNIFI GGTGSGKTTFLNALS FIP ER++TIEDSAELQI V N+V LET Sbjct: 187 LERLVKAKYNIFICGGTGSGKTTFLNALSNFIPKDERIVTIEDSAELQITGVENIVRLET 246 Query: 243 RNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTGHSNS 302 RNANTEG+GEI+IRDLIR+SLRMRP RI+VGEVRG E LDMLQAMNTGHDGSLSTGH+NS Sbjct: 247 RNANTEGKGEITIRDLIRTSLRMRPERIIVGEVRGKEALDMLQAMNTGHDGSLSTGHANS 306 Query: 303 ARDMVSRLETMVLSAADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAGVKEGE 362 +DM+SRLETMVLS A++P+ +RQQI SAIDI +HL RLRD+SRR EI EV K G+ Sbjct: 307 TKDMLSRLETMVLSGAEMPLEAIRQQIASAIDIIIHLGRLRDKSRRTLEITEVVEYKNGQ 366 Query: 363 VVLNPLYEFRETGGT-DGHVQGGLIPSGNPMLHTGKLRMAGIHWK 406 +VLNPLYEF E G T + V G L + N M++ K +MAGI K Sbjct: 367 IVLNPLYEFVEEGETPEKQVIGTLRRTKNEMVNKLKFKMAGISDK 411 >gb|EAI69822.1| unknown [environmental sequence] Length = 631 Score = 353 bits (907), Expect = 3e-96, Method: Compositional matrix adjust. Identities = 176/338 (52%), Positives = 240/338 (71%), Gaps = 3/338 (0%) Query: 53 LVKKLFDSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDII 112 L K +FD L+ L+ +P +TEIM+N HDE+F+E+ G + R P+ F + I Sbjct: 269 LKKSVFDEIIGLGPLEELIADPTVTEIMVNCHDEVFIEQLGRLTRSPVVFTDDRAVLGAI 328 Query: 113 QSVVSGVNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRR 172 + +V+ + R +++SSP+VDARL DGSRVN V+PP+ALKGP++TIRKF + + +LI+ Sbjct: 329 ERIVAPIGRRIDESSPMVDARLADGSRVNAVIPPLALKGPSITIRKFSKQKLAGEDLIQF 388 Query: 173 ETVSMEAAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIV 232 ++S E E LQ V + NI ISGGTGSGKTT LN LS +IP ER++T+ED+AELQ+ Sbjct: 389 GSMSPEMLEFLQTAVEQRANIIISGGTGSGKTTLLNVLSSYIPDDERIVTVEDAAELQL- 447 Query: 233 TVPNLVSLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHD 292 + PNLVSLE R AN EG+G I IRDL+++ LRMRP+RIV+GE RG E LDMLQAMNTGHD Sbjct: 448 SQPNLVSLEARPANMEGKGAIHIRDLVKNCLRMRPDRIVIGECRGGEALDMLQAMNTGHD 507 Query: 293 GSLSTGHSNSARDMVSRLETMVLSAA-DLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTE 351 GSL+T H+N+ RD ++RLE M L A DLPV +R+QI SA+DI V +R SRRVT Sbjct: 508 GSLTTAHANAPRDCIARLEVMTLMAGLDLPVQAIREQICSAVDIIVQQTRFSCGSRRVTH 567 Query: 352 ICEVAGVKEGEVVLNPLYEFRETG-GTDGHVQGGLIPS 388 + EV+G++ G + L ++ FRE G G +G +QG +P+ Sbjct: 568 VTEVSGMESGVITLQDVFVFREEGIGENGKIQGKFVPT 605 >ref|ZP_00029043.1| COG4962: Flp pilus assembly protein, ATPase CpaF [Burkholderia fungorum] Length = 585 Score = 347 bits (891), Expect = 2e-94, Method: Compositional matrix adjust. Identities = 176/356 (49%), Positives = 243/356 (68%), Gaps = 3/356 (0%) Query: 36 VLARDSLRHLTAQEKHTLVKKLFDSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLI 95 +L D+ H L K +FD L+ L+ +P ++EIM+N H+EIF+E+NG + Sbjct: 206 ILNHDASFHTADIPLSALKKSVFDEIIGLGPLEELIADPTVSEIMVNCHNEIFIEQNGQL 265 Query: 96 RRLPLAFESSSRLEDIIQSVVSGVNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMT 155 R P+ F + I+ +V+ + R +++SSP+VDARL DGSRVN V+PP+ALKGP++T Sbjct: 266 TRSPVIFTDDRAVLGAIERIVAPIGRRIDESSPMVDARLSDGSRVNAVIPPLALKGPSIT 325 Query: 156 IRKFPETPMTMNELIRRETVSMEAAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIP 215 IRKF + +LIR ++S + E L V NI ISGGTGSGKTT LN LS +IP Sbjct: 326 IRKFSRQKLGGEDLIRFGSMSPDMLEFLHTAVKEGANIVISGGTGSGKTTLLNVLSSYIP 385 Query: 216 PQERVITIEDSAELQIVTVPNLVSLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEV 275 ER++T+ED+AELQ+ + PNLVSLE R AN EG+G I IRDL+++ LRMRP+RIV+GE Sbjct: 386 DDERIVTVEDAAELQL-SQPNLVSLEARPANMEGKGAIHIRDLVKNCLRMRPDRIVIGEC 444 Query: 276 RGAECLDMLQAMNTGHDGSLSTGHSNSARDMVSRLETMVLSAA-DLPVAVVRQQIGSAID 334 RG E LDMLQAMNTGHDGSL+T H+N+ RD ++RLE M L A DLPV +R+QI SA+D Sbjct: 445 RGGEALDMLQAMNTGHDGSLTTAHANTPRDCIARLEVMTLMAGLDLPVQAIREQICSAVD 504 Query: 335 IFVHLSRLRDRSRRVTEICEVAGVKEGEVVLNPLYEFRETGGTD-GHVQGGLIPSG 389 + V SR SRRVT + EV+G++ G + L ++ F+E G ++ G +QG +P+ Sbjct: 505 LIVQQSRFSCGSRRVTHVTEVSGMESGVITLQDVFVFKEEGFSEHGKIQGKFVPTA 560 >ref|NP_936652.1| TadA protein [Vibrio vulnificus YJ016] dbj|BAC96622.1| TadA protein [Vibrio vulnificus YJ016] Length = 421 Score = 331 bits (848), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 176/387 (45%), Positives = 259/387 (66%), Gaps = 7/387 (1%) Query: 3 EEMFRLLRSDIRSGLDLT--SAVGNRELTAYIEQTV--LARDSLRHLTAQEKHTLVKKLF 58 ++++ LR +I +D + S + N EL + ++V L +++ ++ LVK L+ Sbjct: 5 KQIYLDLRDEIFDAIDASTLSEISNEELAEQLSESVNILIDKKQLQVSSLKRAELVKALY 64 Query: 59 DSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSG 118 D + LQ LV+N I++IMIN ++F+E G + + P+ F + +L I + + S Sbjct: 65 DELKGLGPLQKLVENDDISDIMINGPYDVFIEIGGKVEKSPIQFVNEKQLNTIAKRIASN 124 Query: 119 VNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSME 178 V R +++SSP+ DARLKDGSRVNIV+PP+A+ G +++IRKF E + + L+ +S+E Sbjct: 125 VGRRIDESSPLCDARLKDGSRVNIVIPPLAIDGTSISIRKFKEQKIKLENLVEFGAMSIE 184 Query: 179 AAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLV 238 A+LL + K NI ISGGTGSGKTT LNALS FI ERV+TIED+AELQ+ P++V Sbjct: 185 MAKLLSIASHCKCNILISGGTGSGKTTLLNALSGFIGEGERVVTIEDAAELQLQK-PHIV 243 Query: 239 SLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTG 298 LETR A+ EG G+I+ RDL+ ++LRMRP+RI+VGE RGAE +MLQAMNTGHDGS+ST Sbjct: 244 RLETRQASVEGTGQITARDLVINALRMRPDRIIVGECRGAEAFEMLQAMNTGHDGSMSTL 303 Query: 299 HSNSARDMVSRLETMVLSA-ADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAG 357 H+N+ RD ++R E+MV+ A A LP+ +R+ I SA+D+ V + RL D SR+V I E+ G Sbjct: 304 HANTPRDAIARTESMVMMATASLPLEAIRRTIVSAVDLIVQVRRLHDGSRKVMYISEIVG 363 Query: 358 VKEGEVVLNPLYEFRETGGTD-GHVQG 383 ++ VV+ ++ F TG D G +QG Sbjct: 364 LEGNNVVMEDIFRFETTGSFDGGKIQG 390 >ref|NP_762074.1| Flp pilus assembly protein [Vibrio vulnificus CMCP6] gb|AAO07064.1| Flp pilus assembly protein [Vibrio vulnificus CMCP6] Length = 421 Score = 331 bits (848), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 176/387 (45%), Positives = 259/387 (66%), Gaps = 7/387 (1%) Query: 3 EEMFRLLRSDIRSGLDLT--SAVGNRELTAYIEQTV--LARDSLRHLTAQEKHTLVKKLF 58 ++++ LR +I +D + S + N EL + ++V L +++ ++ LVK L+ Sbjct: 5 KQIYLDLRDEIFDAIDASTLSEISNEELAEQLSESVNILIDKKQLQVSSLKRAELVKALY 64 Query: 59 DSFRXXXXLQPLVDNPAITEIMINSHDEIFVEENGLIRRLPLAFESSSRLEDIIQSVVSG 118 D + LQ LV+N I++IMIN ++F+E G + + P+ F + +L I + + S Sbjct: 65 DELKGLGPLQKLVENDDISDIMINGPYDVFIEIGGKVEKSPIQFVNEKQLNTIAKRIASN 124 Query: 119 VNRVVNDSSPIVDARLKDGSRVNIVLPPVALKGPAMTIRKFPETPMTMNELIRRETVSME 178 V R +++SSP+ DARLKDGSRVNIV+PP+A+ G +++IRKF E + + L+ +S+E Sbjct: 125 VGRRIDESSPLCDARLKDGSRVNIVIPPLAIDGTSISIRKFKEQKIKLENLVEFGAMSIE 184 Query: 179 AAELLQMLVAAKYNIFISGGTGSGKTTFLNALSQFIPPQERVITIEDSAELQIVTVPNLV 238 A+LL + K NI ISGGTGSGKTT LNALS FI ERV+TIED+AELQ+ P++V Sbjct: 185 MAKLLSIASHCKCNILISGGTGSGKTTLLNALSGFIGEGERVVTIEDAAELQLQK-PHIV 243 Query: 239 SLETRNANTEGRGEISIRDLIRSSLRMRPNRIVVGEVRGAECLDMLQAMNTGHDGSLSTG 298 LETR A+ EG G+I+ RDL+ ++LRMRP+RI+VGE RGAE +MLQAMNTGHDGS+ST Sbjct: 244 RLETRQASVEGTGQITARDLVINALRMRPDRIIVGECRGAEAFEMLQAMNTGHDGSMSTL 303 Query: 299 HSNSARDMVSRLETMVLSA-ADLPVAVVRQQIGSAIDIFVHLSRLRDRSRRVTEICEVAG 357 H+N+ RD ++R E+MV+ A A LP+ +R+ I SA+D+ V + RL D SR+V I E+ G Sbjct: 304 HANTPRDAIARTESMVMMATASLPLEAIRRTIVSAVDLIVQVRRLHDGSRKVMYISEIVG 363 Query: 358 VKEGEVVLNPLYEFRETGGTD-GHVQG 383 ++ VV+ ++ F TG D G +QG Sbjct: 364 LEGNNVVMEDIFRFETTGSFDSGKIQG 390 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.135 0.372 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 549,867,370 Number of extensions: 21485593 Number of successful extensions: 85918 Number of sequences better than 1.0e-05: 666 Number of HSP's gapped: 85376 Number of HSP's successfully gapped: 666 Length of query: 410 Length of database: 757,237,578 Length adjustment: 128 Effective length of query: 282 Effective length of database: 409,704,906 Effective search space: 115536783492 Effective search space used: 115536783492 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)