BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0054.p (368 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00183191.1| hypothetical protein Exigu142901 [Exiguobacte... 339 7e-92 ref|NP_692859.1| endonuclease IV [Oceanobacillus iheyensis HTE83... 338 1e-91 ref|NP_764799.1| endonuclease IV [Staphylococcus epidermidis ATC... 337 3e-91 ref|NP_242252.1| endonuclease IV [Bacillus halodurans] >gi|20138... 332 1e-89 ref|NP_372081.1| hypothetical protein [Staphylococcus aureus sub... 330 2e-89 >ref|ZP_00183191.1| hypothetical protein Exigu142901 [Exiguobacterium sp. 255-15] Length = 299 Score = 339 bits (869), Expect = 7e-92, Method: Compositional matrix adjust. Identities = 170/272 (62%), Positives = 211/272 (77%), Gaps = 4/272 (1%) Query: 6 KGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVVHAPYI 65 K LL A+ EA YG+T+ M+YTGAPQNTRRKPI + E M++NG+EEIVVHAPYI Sbjct: 13 KMLLGASEEAASYGATTMMVYTGAPQNTRRKPISDLRIPEALLHMEENGIEEIVVHAPYI 72 Query: 66 INLG-SYKDSTYQLAVDFLQEEIRRTHALE-VKHIVLHPGAYTDKDAEYGVQRIADGLNE 123 INLG + K T++LAV FL EEIRR ALE KHIVLHPGA+ E G++RI +GLNE Sbjct: 73 INLGNTTKPETFELAVSFLGEEIRRAEALEKAKHIVLHPGAHVGAGEEVGIKRIIEGLNE 132 Query: 124 VLGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAGYDI 183 VL T + +V IALETMAGKG+E+G++FEEIA+II V HN+RLS+CLDTCH+HDAGYD+ Sbjct: 133 VL--TGDEKVKIALETMAGKGSEIGKTFEEIAAIIAGVTHNDRLSVCLDTCHVHDAGYDL 190 Query: 184 VGDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVHHEA 243 + DLDGVL+ FD+I+G++RLGVIH+NDSKN RGA KDRH IG G IGF+ ++++VHH Sbjct: 191 IHDLDGVLESFDRIVGINRLGVIHVNDSKNIRGAKKDRHENIGFGEIGFDPLHRIVHHRD 250 Query: 244 LAGLPFILETPWIGKDAKKLRPMYEVEIALLR 275 LA LP ILETP+IG D +K Y EIA+LR Sbjct: 251 LAHLPKILETPYIGLDPRKKVAPYREEIAMLR 282 >ref|NP_692859.1| endonuclease IV [Oceanobacillus iheyensis HTE831] sp|Q8EPZ2|END4_OCEIH Probable endonuclease IV (Endodeoxyribonuclease IV) dbj|BAC13894.1| endonuclease IV [Oceanobacillus iheyensis HTE831] Length = 297 Score = 338 bits (867), Expect = 1e-91, Method: Compositional matrix adjust. Identities = 164/271 (60%), Positives = 210/271 (77%), Gaps = 3/271 (1%) Query: 6 KGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVVHAPYI 65 K LL ++ +A +YG+ +FMIYTGAPQNTRRKPIE + G + MK NG+++IVVHAPYI Sbjct: 14 KMLLGSSEDAVQYGANTFMIYTGAPQNTRRKPIEELNIEAGTEHMKANGIQDIVVHAPYI 73 Query: 66 INLG-SYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADGLNEV 124 IN+G S K +T++L V+FL+ EI RT AL K IVLHPGA+ + AE G+ +I +GLNEV Sbjct: 74 INIGNSIKPATFELGVNFLKNEIDRTEALGAKQIVLHPGAHVGEGAEKGIPKIIEGLNEV 133 Query: 125 LGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAGYDIV 184 L + V IALETMAGKG+E+GR+FEE+A II+ V HN+RLSIC+DTCHIHDAGY+IV Sbjct: 134 LDPN--SNVQIALETMAGKGSEIGRTFEELAQIIEGVTHNDRLSICMDTCHIHDAGYNIV 191 Query: 185 GDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVHHEAL 244 D DGVL+QFD+IIG+ RL V+H+NDSKN RGA KDRH IG GYIGFE ++ +VHH L Sbjct: 192 EDFDGVLEQFDKIIGIDRLKVVHVNDSKNERGAHKDRHENIGFGYIGFEALHNIVHHPQL 251 Query: 245 AGLPFILETPWIGKDAKKLRPMYEVEIALLR 275 + LP ILETP++G D K +P Y+ EI +L+ Sbjct: 252 SDLPKILETPFVGTDKKNKKPPYKHEIEMLK 282 >ref|NP_764799.1| endonuclease IV [Staphylococcus epidermidis ATCC 12228] sp|Q8CP25|END4_STAEP Probable endonuclease IV (Endodeoxyribonuclease IV) gb|AAO04843.1| endonuclease IV [Staphylococcus epidermidis ATCC 12228] Length = 296 Score = 337 bits (864), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 166/292 (56%), Positives = 217/292 (74%), Gaps = 7/292 (2%) Query: 1 VSCADKGLLS-AANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIV 59 VS + K +L +A EA++YG ++FMIYTGAPQNTRRK IE + +G+QAMK G+ IV Sbjct: 7 VSMSGKKMLQGSAEEAHKYGESTFMIYTGAPQNTRRKNIEDLNIEKGQQAMKTYGLSNIV 66 Query: 60 VHAPYIINLG-SYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIA 118 VHAPYIIN+ + K + L VDFLQ+EI RT AL K IVLHPGA+ + G+Q+I Sbjct: 67 VHAPYIINIANTTKPEVFNLGVDFLQKEIERTQALGAKDIVLHPGAHVGAGVDKGIQKII 126 Query: 119 DGLNEVLGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHD 178 +GLNEVL T++ +V IALETMAGKGTEVGRSFEEIA IID V HN+RLS+C DTCH HD Sbjct: 127 EGLNEVL--THDNDVRIALETMAGKGTEVGRSFEEIAQIIDGVTHNDRLSVCFDTCHTHD 184 Query: 179 AGYDIVGDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKV 238 AGY++ D DGVL++FD IIG+ R+ V+H+NDSKN RGA KDRH IG G+IGF+ +N V Sbjct: 185 AGYNVKEDFDGVLEKFDSIIGVDRIKVVHVNDSKNLRGAQKDRHENIGFGHIGFDALNYV 244 Query: 239 VHHEALAGLPFILETPWIGKDAKKLRPMYEVEIALLRGTVAERFGAEFLNEV 290 VHH+ +P ILETP++G+D K +P Y++EI +L+ +++F E N++ Sbjct: 245 VHHDTFKNIPKILETPYVGEDKKNKKPPYKLEIDMLK---SQKFDPELKNKI 293 >ref|NP_242252.1| endonuclease IV [Bacillus halodurans] sp|Q9KD33|END4_BACHD Probable endonuclease IV (Endodeoxyribonuclease IV) pir||B83823 endonuclease IV BH1386 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05105.1| endonuclease IV [Bacillus halodurans] Length = 298 Score = 332 bits (850), Expect = 1e-89, Method: Compositional matrix adjust. Identities = 162/277 (58%), Positives = 207/277 (74%), Gaps = 4/277 (1%) Query: 6 KGLLSAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIVVHAPYI 65 K LL ++ EA YG+ +FM+YTGAPQNTRRKPIE + G+ MK+NG++ I+VHAPYI Sbjct: 15 KMLLGSSEEAISYGANTFMVYTGAPQNTRRKPIEELNIEAGRAHMKENGIDHIIVHAPYI 74 Query: 66 INLG-SYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIADGLNEV 124 IN+G S K +T++L VDFLQ EI RT AL IVLHPGA+ + G+++I +GLNEV Sbjct: 75 INIGNSEKPATFKLGVDFLQSEIERTQALGADQIVLHPGAHVGAGVDKGIEKIIEGLNEV 134 Query: 125 LGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHDAGYDIV 184 L T V IALETMAGKG+E GR+FEEIA II+ V HN+RLS+C DTCH HDAGY+IV Sbjct: 135 L--TENDGVQIALETMAGKGSECGRTFEEIARIINGVTHNDRLSVCFDTCHTHDAGYNIV 192 Query: 185 GDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKVVHHEAL 244 D DGVL +FD+IIG+ R+ V+HINDSKNPRGA KDRH IG G+IGF+ ++ +VHH L Sbjct: 193 EDFDGVLNEFDKIIGVERIKVLHINDSKNPRGAAKDRHENIGFGHIGFKALHYIVHHPQL 252 Query: 245 AGLPFILETPWIGKDAKKLRPMYEVEIALLR-GTVAE 280 +P ILETP++G+D K +P Y+ EI ++R GT E Sbjct: 253 QDIPKILETPYVGEDKKNKKPPYKFEIDMIRNGTFHE 289 >ref|NP_372081.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] ref|NP_374670.1| hypothetical protein, similar to endonuclease IV [Staphylococcus aureus subsp. aureus N315] ref|NP_646326.1| ORFID:MW1509~hypothetical protein, similar to endonuclease IV [Staphylococcus aureus subsp. aureus MW2] sp|Q99TT9|END4_STAAM Probable endonuclease IV (Endodeoxyribonuclease IV) pir||D89936 hypothetical protein SA1386 [imported] - Staphylococcus aureus (strain N315) dbj|BAB42649.1| SA1386 [Staphylococcus aureus subsp. aureus N315] dbj|BAB57719.1| hypothetical protein [Staphylococcus aureus subsp. aureus Mu50] dbj|BAB95374.1| MW1509 [Staphylococcus aureus subsp. aureus MW2] Length = 296 Score = 330 bits (847), Expect = 2e-89, Method: Compositional matrix adjust. Identities = 164/292 (56%), Positives = 213/292 (72%), Gaps = 7/292 (2%) Query: 1 VSCADKGLL-SAANEANEYGSTSFMIYTGAPQNTRRKPIESMYPAEGKQAMKDNGVEEIV 59 VS + K +L +A EA EYG T+FMIYTGAPQNTRRK IE + +G + M+ G+ IV Sbjct: 7 VSMSGKKMLEGSAIEAYEYGETTFMIYTGAPQNTRRKSIEDLNITKGHEVMEKYGLSNIV 66 Query: 60 VHAPYIINLG-SYKDSTYQLAVDFLQEEIRRTHALEVKHIVLHPGAYTDKDAEYGVQRIA 118 VHAPYIIN+ + K T+ L VDFLQ+EI RT A+ K IVLHPGA+ + G+ +I Sbjct: 67 VHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIGAKDIVLHPGAHVGAGVDAGINKII 126 Query: 119 DGLNEVLGGTNETEVHIALETMAGKGTEVGRSFEEIASIIDKVVHNERLSICLDTCHIHD 178 +GLNEVL TN+ V IALETMAGKGTE+GRSFEE+A IID V +NERLS+C DTCH HD Sbjct: 127 EGLNEVL--TNDNNVRIALETMAGKGTEIGRSFEELARIIDGVHNNERLSVCFDTCHTHD 184 Query: 179 AGYDIVGDLDGVLQQFDQIIGLSRLGVIHINDSKNPRGAGKDRHTPIGSGYIGFETINKV 238 AGY++ D DGVL +FD+IIG+ R+ V+H+NDSKN RGA KDRH IG GYIGF+ +N + Sbjct: 185 AGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSKNDRGAQKDRHENIGFGYIGFDALNYI 244 Query: 239 VHHEALAGLPFILETPWIGKDAKKLRPMYEVEIALLRGTVAERFGAEFLNEV 290 VHH++ +P ILETP++G+D K +P Y++EI +L+ ++F E N+V Sbjct: 245 VHHDSFKDIPKILETPYVGEDKKNKKPPYKLEIEMLK---QQQFDPELKNKV 293 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.137 0.398 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 550,538,720 Number of extensions: 23824028 Number of successful extensions: 49183 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 48946 Number of HSP's successfully gapped: 140 Length of query: 368 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 241 Effective length of database: 412,420,005 Effective search space: 99393221205 Effective search space used: 99393221205 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)