BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0037.p (537 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00059746.1| COG2866: Predicted carboxypeptidase [Clostrid... 212 2e-53 ref|NP_781274.1| gamma-D-glutamyl-L-diamino acid endopeptidase I... 204 6e-51 ref|NP_242469.1| gamma-D-glutamyl-L-diamino acid endopeptidase I... 178 3e-43 sp|Q03415|ENP1_BACSH Gamma-D-glutamyl-L-diamino acid endopeptida... 172 1e-41 ref|NP_692652.1| gamma-D-glutamyl-L-diamino acid endopeptidase I... 164 6e-39 >ref|ZP_00059746.1| COG2866: Predicted carboxypeptidase [Clostridium thermocellum ATCC 27405] Length = 429 Score = 212 bits (540), Expect = 2e-53, Method: Compositional matrix adjust. Identities = 117/300 (39%), Positives = 174/300 (58%), Gaps = 14/300 (4%) Query: 51 YSYTVMQRDIERLVKEYPDLVSTESLGQTAYGRQLWAVKLGRGESVLFLNGSHHAREWMT 110 Y+Y +M+RDI+ L YP + T G + GR L+ ++LG G +F N +HHA EW+T Sbjct: 136 YTYEIMERDIQGLKARYP-FLETGVAGTSVLGRNLYYLRLGTGPRQVFYNAAHHAIEWIT 194 Query: 111 SALLMKMIDTYAQAYYSNGKIADYNVRSLLDEVSIWVVPMVNPDGVTLSQQGTAGLPVN- 169 + LLMK + + +AY + +I YNVR + ++ SI++VPMVNPDGV L G + P N Sbjct: 195 TVLLMKFAENFLKAYSTGSRIRGYNVREIWNQSSIYIVPMVNPDGVDLVLNGLS--PTNP 252 Query: 170 LAQTLRRYNGNSTNFNR-WKANMQGIDLNRQYPARWTTIQD-----AVKYPWYQNYKGQK 223 L R+N F++ W AN++G+DLNR YPA W + + P Y G Sbjct: 253 YYADLLRWNTTGRPFSQVWSANIRGVDLNRNYPASWEEAKAQEEALGIFGPGPTRYGGPY 312 Query: 224 PGQAPEVQMMMDFTHKVDPEVTISYHSSGEIIFWHFNTLNSNLARDKTMARALGNLTGYS 283 P PE M+ FT D + ++YHS G +I+W N LN T+A A ++GY Sbjct: 313 PLSEPESSAMVSFTRTHDFRLALAYHSQGRVIYW--NYLNLAPPESLTIANAFARVSGYI 370 Query: 284 LVIPQKNPSGGGYKDWFIQEYGRPGFTIEIANYAGESSVPLRQFSGIWSENKEVGLYSAL 343 ++ + GYKDWFIQEY RPGFTIE+ G++ +P+ QF+ I+++N+E+ L ++L Sbjct: 371 VLDVPYEAAYAGYKDWFIQEYRRPGFTIEVG--LGQNPLPISQFNTIYNDNEEILLLASL 428 >ref|NP_781274.1| gamma-D-glutamyl-L-diamino acid endopeptidase I [Clostridium tetani E88] gb|AAO35211.1| gamma-D-glutamyl-L-diamino acid endopeptidase I [Clostridium tetani E88] Length = 423 Score = 204 bits (518), Expect = 6e-51, Method: Compositional matrix adjust. Identities = 110/306 (35%), Positives = 177/306 (57%), Gaps = 12/306 (3%) Query: 44 IVNPNQVYSYTVMQRDIERLVKEYPDLVSTESLGQTAYGRQLWAVKLGRGESVLFLNGSH 103 +V + Y+Y +++RDI L + YP + S+G++ GR+L+ +KLG G + +F NG+H Sbjct: 123 VVQTDIDYTYEILERDINGLKRRYP-FIEVGSIGKSVLGRELYYLKLGNGPNKVFYNGAH 181 Query: 104 HAREWMTSALLMKMIDTYAQAYYSNGKIADYNVRSLLDEVSIWVVPMVNPDGVTLSQQGT 163 H+ EW+T+ LLMK + + ++Y ++ YN+ + +I++VPMVNPDGV L G Sbjct: 182 HSLEWITTPLLMKFAENFIRSYAFGEEMRGYNIGDIWSRSTIYIVPMVNPDGVDLVLNGL 241 Query: 164 AGLPVNLAQTLRRYNGNSTNFN-RWKANMQGIDLNRQYPARWTTIQDAVKY-----PWYQ 217 L +N ST+F+ W+AN +G+DLN Y A W +DA K P Sbjct: 242 K-RDNPYYDELIVWNNGSTDFSTNWQANNRGVDLNHNYNASWQKSKDAEKEYGIYGPGPT 300 Query: 218 NYKGQKPGQAPEVQMMMDFTHKVDPEVTISYHSSGEIIFWHFNTLNSNLARDKTMARALG 277 Y G P PE Q M+ FT + D +T++YHS GE+I+W ++TL + + Sbjct: 301 RYSGPYPESEPETQAMVKFTREHDFRLTLAYHSQGEVIYWQYSTLTP--PESERIVEIFA 358 Query: 278 NLTGYSLVIPQKNPSGGGYKDWFIQEYGRPGFTIEIANYAGESSVPLRQFSGIWSENKEV 337 ++GY+ + S GYKDWFI+E+ RPGFTIE+ G++ +P+ QF I+ +N+E+ Sbjct: 359 EVSGYTPSQTEGIASYAGYKDWFIEEFRRPGFTIEVG--LGKNPLPISQFDKIYEDNEEL 416 Query: 338 GLYSAL 343 L +++ Sbjct: 417 LLIASV 422 >ref|NP_242469.1| gamma-D-glutamyl-L-diamino acid endopeptidase I [Bacillus halodurans] pir||C83850 gamma-D-glutamyl-L-diamino acid endopeptidase I BH1603 [imported] - Bacillus halodurans (strain C-125) dbj|BAB05322.1| gamma-D-glutamyl-L-diamino acid endopeptidase I [Bacillus halodurans] Length = 355 Score = 178 bits (452), Expect = 3e-43, Method: Compositional matrix adjust. Identities = 104/290 (35%), Positives = 156/290 (53%), Gaps = 11/290 (3%) Query: 51 YSYTVMQRDIERLVKEYPDLVSTESLGQTAYGRQLWAVKLGRGESVLFLNGSHHAREWMT 110 Y YT + D RL ++ V +G++ G+++ A ++G GE +F +G HA EW T Sbjct: 67 YGYTELLLDRNRLKQQ---RVKESVIGKSVMGKEIVAFEIGSGEIPIFYSGGWHANEWHT 123 Query: 111 SALLMKMIDTYAQAYYSNGKIADYNVRSLLDEVSIWVVPMVNPDGVTLSQQGTAGLPVNL 170 + LM + T +A S + Y+V LL +V++ +VP+VNPDGV L QQG Sbjct: 124 AKFLMTFLQTLIEAEVSKADLFGYSVYELLKKVTLHLVPLVNPDGVELVQQGIYPEHPFY 183 Query: 171 AQTLRRYNGNSTNFNRWKANMQGIDLNRQYPARW-TTIQDAVKYPWYQNYKGQKPGQAPE 229 Q L G T F+ W +N++G+DLN Q+PA W ++ + PW ++Y G P PE Sbjct: 184 EQVLAINKGART-FDHWSSNIRGVDLNHQWPAGWEKEAAESPQQPWPRHYSGPSPLSEPE 242 Query: 230 VQMMMDFTHKVDPEVTISYHSSGEIIFWHFNTLNSNLARDKTMARALGNLTGYSLVIPQK 289 + ++T D +++HS G+ I+W + N++ K M L ++ Y V Sbjct: 243 AIAIYEYTKANDFAYVLAFHSQGQEIYWGYKGFEPNVS--KAMVERLSLMSSYKPV--HT 298 Query: 290 NPSGGGYKDWFIQEYGRPGFTIEIANYAGESSVPLRQFSGIWSENKEVGL 339 S GGYKDWFIQE RPGFTIE+ G++ +PL F IWS N + L Sbjct: 299 ADSDGGYKDWFIQETRRPGFTIEVG--VGKNPLPLEAFGEIWSNNILLAL 346 >sp|Q03415|ENP1_BACSH Gamma-D-glutamyl-L-diamino acid endopeptidase I (Gamma-D-glutamyl-meso-diaminopimelate peptidase I) (Endopeptidase I) pir||S33310 endopeptidase I - Bacillus sphaericus emb|CAA49259.1| endopeptidase I [Bacillus sphaericus] Length = 396 Score = 172 bits (437), Expect = 1e-41, Method: Compositional matrix adjust. Identities = 102/303 (33%), Positives = 165/303 (54%), Gaps = 17/303 (5%) Query: 44 IVNPNQVYSYTVMQRDIERLVKEYPDLVSTESLGQTAYGRQLWAVKLGRGESVLFLNGSH 103 +VN Q Y Y++M DI++L YP L T +G + + + + +G G + S Sbjct: 103 LVNGQQNYDYSMMMNDIKKLQTAYPFLQGT-PIGNSVLAQPIPEILIGNGSKRIHYKASF 161 Query: 104 HAREWMTSALLMKMIDTYAQAYYSNGKIADYNVRSLLDEVSIWVVPMVNPDGVTLSQQGT 163 HA EW+T+ ++M ++ Y A + I ++ L ++ ++ +VPMVNPDGV L G Sbjct: 162 HANEWITTPIIMTFLNDYLLALTNQTTIRGLSMGPLYNQTTLSLVPMVNPDGVNLVINGP 221 Query: 164 AGLPVN--LAQTLRRYNGNSTNFNRWKANMQGIDLNRQYPARWTTIQDA--VKYPWYQNY 219 P N L L +N NS NF+ WKAN+ G+DLN Q+PA+W +++A + P ++Y Sbjct: 222 ---PANEALKNKLIAWNHNSQNFSGWKANINGVDLNDQFPAKW-ELENARNPQTPGPRDY 277 Query: 220 KGQKPGQAPEVQMMMDFTHKVDPEVTISYHSSGEIIFWHFNTLNSNLARDKTMARALGNL 279 G+ P PE M D T + +++H+ G +I+W F L +TM + Sbjct: 278 GGEAPLTQPEAIAMADLTRSRNFAWVLAFHTQGRVIYWGFENLEP--PESQTMVEEFSRV 335 Query: 280 TGYSLVIPQKNPSGGGYKDWFIQEYGRPGFTIEIANYAGESSVPLRQFSGIWSENKEVGL 339 +GY + Q S GYKDWFIQ++ RPGFT+E+ +G + +P+ +F I+ E +G+ Sbjct: 336 SGYEPI--QSANSYAGYKDWFIQDWRRPGFTVELG--SGTNPLPISEFDTIYQE--ALGI 389 Query: 340 YSA 342 + A Sbjct: 390 FLA 392 >ref|NP_692652.1| gamma-D-glutamyl-L-diamino acid endopeptidase I [Oceanobacillus iheyensis HTE831] dbj|BAC13687.1| gamma-D-glutamyl-L-diamino acid endopeptidase I [Oceanobacillus iheyensis HTE831] Length = 396 Score = 164 bits (414), Expect = 6e-39, Method: Compositional matrix adjust. Identities = 99/292 (33%), Positives = 150/292 (51%), Gaps = 11/292 (3%) Query: 44 IVNPNQVYSYTVMQRDIERLVKEYPDLVSTESLGQTAYGRQLWAVKLGRGESVLFLNGSH 103 +VN Y+Y +D+ L + YP + +G + G+ + +++G G + +NGS Sbjct: 103 LVNDIDQYTYEKFIQDLILLQQVYP-FIQQRYIGNSVLGKDIIELQIGAGSKQVHMNGSF 161 Query: 104 HAREWMTSALLMKMIDTYAQAYYSNGKIADYNVRSLLDEVSIWVVPMVNPDGVTLSQQGT 163 HA EW+T+ +LMK I+ Y A + I + + + +VPMVNPDGV L G Sbjct: 162 HANEWITTPVLMKFINEYVLALTNGTAIRGLPMLPFYQQTQLSIVPMVNPDGVDLVVLG- 220 Query: 164 AGLPVNLAQTLRRYNGNSTNFNRWKANMQGIDLNRQYPARWTT-IQDAVKYPWYQNYKGQ 222 A +++ N +T+F+ WKAN++G+DLN QYPA W + P +++ G Sbjct: 221 ADAAGEFRESVLEINNQNTDFSDWKANIRGVDLNNQYPALWDVEAERKPTSPQPRDFPGY 280 Query: 223 KPGQAPEVQMMMDFTHKVDPEVTISYHSSGEIIFWHFNTLNSNLARDKTMARALGNLTGY 282 P PE + + T D + H+ GE+IFW F L T+ ++GY Sbjct: 281 APLTEPEAIAIAELTRDRDFSRINALHTQGEVIFWGFEGLEP--PESLTIVTEFERVSGY 338 Query: 283 SLVIP-QKNPSGGGYKDWFIQEYGRPGFTIEIANYAGESSVPLRQFSGIWSE 333 IP Q S GYKDWFIQEY RPGFT+E+ G + +P+ QF I+ E Sbjct: 339 ---IPIQYVDSYAGYKDWFIQEYRRPGFTLELGQ--GTNPLPISQFDEIYEE 385 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.132 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 752,837,109 Number of extensions: 30841863 Number of successful extensions: 74540 Number of sequences better than 1.0e-05: 183 Number of HSP's gapped: 74319 Number of HSP's successfully gapped: 183 Length of query: 537 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 407 Effective length of database: 404,274,708 Effective search space: 164539806156 Effective search space used: 164539806156 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)