BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0030.p (193 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_420005.1| transcriptional regulator, TetR family [Cauloba... 74 2e-12 ref|NP_421912.1| transcriptional regulator, TetR family [Cauloba... 71 1e-11 gb|EAJ36104.1| unknown [environmental sequence] 67 2e-10 ref|NP_419549.1| transcriptional regulator, TetR family [Cauloba... 67 2e-10 ref|NP_629051.1| putative tetR-family transcriptional regulator ... 66 4e-10 >ref|NP_420005.1| transcriptional regulator, TetR family [Caulobacter crescentus CB15] pir||A87397 transcription regulator, TetR family [imported] - Caulobacter crescentus gb|AAK23173.1| transcriptional regulator, TetR family [Caulobacter crescentus CB15] Length = 211 Score = 73.6 bits (179), Expect = 2e-12, Method: Compositional matrix adjust. Identities = 46/164 (28%), Positives = 77/164 (46%), Gaps = 5/164 (3%) Query: 10 LSRERILEAARHLFITKGYRAISMRSIGQHLGYSHGSLYYHFKEKAELFYAIVVEDFNHV 69 L R IL AA +FI +GY ++R I +G S +LY HF++K ++ I + + Sbjct: 21 LRRGEILAAAEKIFIAEGYAGATIRKIADAVGVSSTALYMHFRDKDQILLEISNDAIERL 80 Query: 70 ATLLNQVMHTPPEPGMTRVEQLILEFIRFGLDHPYQYEIMFMIRDE----ELLAYCRAEQ 125 L Q+ P +P ++RV ++ ++RF LD+P Y+++F + + + A Sbjct: 81 LDLNIQIAGQPIDP-LSRVRLMLEAYMRFALDNPNTYQLVFCSTGDHVKSDKIGETMALG 139 Query: 126 GRCFELFASIVRSHMKEEGYVSEDWQSIPLTLFLSAHGFISYYI 169 RCFE F+ V + S L+ HG +S I Sbjct: 140 DRCFEKFSEPVHEIAAQGRLRSGSAVQAAEVLWSGCHGLVSLLI 183 >ref|NP_421912.1| transcriptional regulator, TetR family [Caulobacter crescentus CB15] pir||D87635 transcription regulator, TetR family [imported] - Caulobacter crescentus gb|AAK25080.1| transcriptional regulator, TetR family [Caulobacter crescentus CB15] Length = 210 Score = 71.2 bits (173), Expect = 1e-11, Method: Compositional matrix adjust. Identities = 55/189 (29%), Positives = 84/189 (44%), Gaps = 9/189 (4%) Query: 12 RERILEAARHLFITKGYRAISMRSIGQHLGYSHGSLYYHFKEKAELFYAIVVEDFNHVAT 71 RE IL+AA LF KG +A+S R I + G S +LY +F K ++ + V F + Sbjct: 20 REEILDAALTLFSQKGVQAVSTRQIAEIAGISQPALYAYFATKDDIAAELCVRAFAILGQ 79 Query: 72 LLNQVMHTPPEPGMTRVEQLILEFIRFGLDHPYQYEIMFMIRDEELLAYCRAEQGR---- 127 + V P E+ + +I FGLDHP Y + FM+ ++ GR Sbjct: 80 RMEAV-RVDYVPTAEHFERCLRVYIDFGLDHPDAYRVAFMLEKSVDGSFLEKTGGRPMIA 138 Query: 128 ---CFELFASIVRSHMKEEGYVSEDWQSIPLTLFLSAHGFISYYI-QDKVSFEDVRQAAV 183 F +FA IV VS+D + +L+ HG +S I + + + D Q Sbjct: 139 GQAVFAVFAEIVAQFHARGEIVSDDAIAATQSLWAGLHGLVSLLIARPEFPWVDREQLIA 198 Query: 184 MHVKVLSRS 192 HV +L + Sbjct: 199 THVAMLRKG 207 >gb|EAJ36104.1| unknown [environmental sequence] Length = 213 Score = 67.4 bits (163), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 60/194 (30%), Positives = 94/194 (48%), Gaps = 28/194 (14%) Query: 1 MARRAVEQEL-SRERILEAARHLFITKGYRAISMRSIGQHLGYSHGSLYYHFKEKAELFY 59 M RR E+ L +R RIL+AA H+F KG S+ I QH G + G++Y+HF K+ELF Sbjct: 1 MVRRTKEEALETRNRILDAAEHVFFEKGVSHTSLADIAQHAGVTRGAIYWHFANKSELFD 60 Query: 60 AIVVEDFNHVATLLNQVMHTPPE-PGMTRVEQL--ILEFIRFGLDHPYQ----YEIMFM- 111 A+ F+ V ++++ P + PG +E++ IL + G+ Q + I+FM Sbjct: 61 AM----FDRVFLPIDELKRMPLDAPGGNPLEKIRQILIWCLLGVQRDAQLRRVFSILFMK 116 Query: 112 ---IRD-EELLAYCRAEQGRCFEL----FASIVRSHMKEEGYVSEDWQSIPLTLFLSAHG 163 + D E LL RA A VR + E + W++ TL L H Sbjct: 117 CEYVADLEPLLQRNRAGMSEALHAIDADLAGAVRLKLLPERL--DTWRA---TLML--HT 169 Query: 164 FISYYIQDKVSFED 177 +S +++D + D Sbjct: 170 LVSGFVRDMLMLPD 183 >ref|NP_419549.1| transcriptional regulator, TetR family [Caulobacter crescentus CB15] pir||A87340 transcription regulator, TetR family [imported] - Caulobacter crescentus gb|AAK22717.1| transcriptional regulator, TetR family [Caulobacter crescentus CB15] Length = 215 Score = 67.4 bits (163), Expect = 2e-10, Method: Compositional matrix adjust. Identities = 37/124 (29%), Positives = 68/124 (54%), Gaps = 7/124 (5%) Query: 1 MARRAVEQELSRERILEAARHLFITKGYRAISMRSIGQHLGYSHGSLYYHFKEKAELFYA 60 + RRA+ ++ +RER+L AAR LF +GY ++R I Q G S G+++ F +K+ELF Sbjct: 18 LTRRALAKQRTRERVLAAARRLFSERGYEGATIRDIAQAAGMSTGAVFASFADKSELFEE 77 Query: 61 IVVEDFNHVATLLNQVMHTPPEPGMTRVEQLILEFIRFGLDHPYQYEIMFMIRDEELLAY 120 I+ D+ + + Q T V++ +L FG+ + + E + ++R +++ Sbjct: 78 ILTADYEVIYAQMIQAARTAKT-----VDEALLGL--FGVAYSFHVEQLPLLRASISVSW 130 Query: 121 CRAE 124 R+E Sbjct: 131 TRSE 134 >ref|NP_629051.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] emb|CAC33063.1| putative tetR-family transcriptional regulator [Streptomyces coelicolor A3(2)] Length = 193 Score = 65.9 bits (159), Expect = 4e-10, Method: Compositional matrix adjust. Identities = 48/163 (29%), Positives = 74/163 (45%), Gaps = 15/163 (9%) Query: 12 RERILEAARHLFITKGYRAISMRSIGQHLGYSHGSLYYHFKEKAELFYAIVVEDFNHVAT 71 R +L AA + + G AIS+R + + SH + +HFK+KA L AI E F +A Sbjct: 13 RRSVLSAALEVIRSDGVAAISLRDLARRAEVSHAAPAHHFKDKAGLLTAIATEGFELLAA 72 Query: 72 LLNQVMHTPPEPGMTRVEQLILEFIRFGLDHPYQYEIMFM-----IRDEELLAYCRAEQG 126 L V P R+ ++ + ++ F LDHP +++MF + D EL A + Sbjct: 73 ALTAV----PAGTTHRLREMGVRYVEFALDHPAHFDVMFRPQLLHVGDPELT----AARE 124 Query: 127 RCFELFASIVRSHMKEEGYVSEDWQSIPLTLFLSAHGFISYYI 169 R AS V + D + LT + AHGF + + Sbjct: 125 RASRALASGVED--LPDMRAGLDARRAGLTAWSLAHGFATLQL 165 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.326 0.139 0.407 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 261,850,212 Number of extensions: 9727185 Number of successful extensions: 33032 Number of sequences better than 1.0e-05: 93 Number of HSP's gapped: 33097 Number of HSP's successfully gapped: 93 Length of query: 193 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 74 Effective length of database: 434,140,797 Effective search space: 32126418978 Effective search space used: 32126418978 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 122 (51.6 bits)