BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0029.p (133 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_391006.1| similar to hypothetical proteins [Bacillus subt... 195 3e-49 ref|NP_244365.1| BH3498~unknown conserved protein [Bacillus halo... 171 6e-42 ref|NP_561487.1| conserved hypothetical protein [Clostridium per... 159 3e-38 ref|ZP_00059848.1| COG0432: Uncharacterized conserved protein [C... 153 1e-36 ref|NP_623742.1| conserved hypothetical protein [Thermoanaerobac... 153 2e-36 >ref|NP_391006.1| similar to hypothetical proteins [Bacillus subtilis] sp|O05243|YUGU_BACSU Hypothetical protein yugU pir||A70012 conserved hypothetical protein yugU - Bacillus subtilis emb|CAB07927.1| unknown [Bacillus subtilis] emb|CAB15106.1| yugU [Bacillus subtilis subsp. subtilis str. 168] Length = 142 Score = 195 bits (495), Expect = 3e-49, Method: Compositional matrix adjust. Identities = 84/131 (64%), Positives = 107/131 (81%) Query: 1 MLHTMEISTSKRDELRDITREVISYVKKSGVQNGIIIVYCPHTTAGIAINENADPDVKHD 60 ML T++I T+KRDE+ DITREV ++++++G+ +G ++YCPHTTAGI INENADPDVK D Sbjct: 11 MLKTLQIKTTKRDEMIDITREVEAFLQETGITSGAALIYCPHTTAGITINENADPDVKKD 70 Query: 61 VLMRLDEVYPWEHPKYRHAEGNTASHLKSITVGPSQSIIIHEGALLLGRWQGIYFCEFDG 120 +L R DEVYPWEH RH EGNTA+H+KS TVG SQ +I+ G L+LG WQGIYFCEFDG Sbjct: 71 MLRRFDEVYPWEHELDRHMEGNTAAHMKSSTVGASQHVIVENGRLILGTWQGIYFCEFDG 130 Query: 121 PRRRQCYLKII 131 PR R CY+K++ Sbjct: 131 PRTRTCYIKMM 141 >ref|NP_244365.1| BH3498~unknown conserved protein [Bacillus halodurans] pir||B84087 hypothetical protein BH3498 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07217.1| BH3498~unknown conserved protein [Bacillus halodurans] Length = 133 Score = 171 bits (432), Expect = 6e-42, Method: Compositional matrix adjust. Identities = 75/131 (57%), Positives = 102/131 (77%), Gaps = 1/131 (0%) Query: 2 LHTMEISTSKRDELRDITREVISYVKKSGVQNGIIIVYCPHTTAGIAINENADPDVKHDV 61 + T ++T RDE+ DIT ++ ++++++GV NG+ IV HTTAGI +NENADPDVK D+ Sbjct: 1 MKTFHLTTQSRDEMVDITSQIETWIRETGVTNGVAIVSSLHTTAGITVNENADPDVKRDM 60 Query: 62 LMRLDEVYPWEHPKYRHAEGNTASHLKSITVGPSQSIIIHEGALLLGRWQGIYFCEFDGP 121 +MRLDEVYPW H RH EGNTA+HLK+ TVG +Q++II EG L+LG WQG+YFCEFDGP Sbjct: 61 IMRLDEVYPWHHENDRHMEGNTAAHLKTSTVGHAQTLIISEGRLVLGTWQGVYFCEFDGP 120 Query: 122 R-RRQCYLKII 131 R R+ +K++ Sbjct: 121 RTNRKFVVKLL 131 >ref|NP_561487.1| conserved hypothetical protein [Clostridium perfringens] dbj|BAB80277.1| conserved hypothetical protein [Clostridium perfringens str. 13] Length = 135 Score = 159 bits (401), Expect = 3e-38, Method: Compositional matrix adjust. Identities = 73/131 (55%), Positives = 95/131 (72%), Gaps = 2/131 (1%) Query: 3 HTMEISTSKRDELRDITREVISYVKKSGVQNGIIIVYCPHTTAGIAINENADPDVKHDVL 62 HT+E T + + IT + +K S ++NGI++VYCPHTTAGI INENADPDV D++ Sbjct: 7 HTLETHTPQ--SMSKITSYIREDIKNSNIENGIVVVYCPHTTAGITINENADPDVVKDLI 64 Query: 63 MRLDEVYPWEHPKYRHAEGNTASHLKSITVGPSQSIIIHEGALLLGRWQGIYFCEFDGPR 122 ++VYP KYRH EGN+ SHLKS TVG SQ +I+H G L+LG+WQ +YFC+FDGPR Sbjct: 65 YGFEKVYPTNDHKYRHFEGNSHSHLKSSTVGVSQQLILHRGRLILGQWQDVYFCDFDGPR 124 Query: 123 RRQCYLKIIEG 133 R Y+KI+EG Sbjct: 125 NRTFYVKILEG 135 >ref|ZP_00059848.1| COG0432: Uncharacterized conserved protein [Clostridium thermocellum ATCC 27405] Length = 130 Score = 153 bits (387), Expect = 1e-36, Method: Compositional matrix adjust. Identities = 72/130 (55%), Positives = 92/130 (70%), Gaps = 1/130 (0%) Query: 1 MLHTMEISTSKRDELRDITREVISYVKKSGVQNGIIIVYCPHTTAGIAINENADPDVKHD 60 ML + I TS R + DIT +V V++SGV++GI V+ PHTTAGI INENADPDV D Sbjct: 1 MLERLSIRTSARTAMIDITSKVQQVVERSGVKSGICTVFVPHTTAGITINENADPDVVSD 60 Query: 61 VLMRLDEVYPWEHPKYRHAEGNTASHLKSITVGPSQSIIIHEGALLLGRWQGIYFCEFDG 120 +L ++++ P+E Y H EGN+A+H+K+ G SQ III LLLG WQGIYFCEFDG Sbjct: 61 ILTEINKIVPFED-GYSHGEGNSAAHIKASLFGSSQQIIIENSRLLLGTWQGIYFCEFDG 119 Query: 121 PRRRQCYLKI 130 PR R+ Y+KI Sbjct: 120 PRNREVYVKI 129 >ref|NP_623742.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] gb|AAM25346.1| conserved hypothetical protein [Thermoanaerobacter tengcongensis] Length = 130 Score = 153 bits (386), Expect = 2e-36, Method: Compositional matrix adjust. Identities = 71/130 (54%), Positives = 97/130 (74%), Gaps = 2/130 (1%) Query: 2 LHTMEISTSKRDELRDITREVISYVKKSGVQNGIIIVYCPHTTAGIAINENADPDVKHDV 61 + ++ I+T R+ + DIT+EV VKKSGV+ GI +V+ PHTTAGI INENADP VK D+ Sbjct: 1 MQSVYINTPLREVMIDITKEVEEEVKKSGVKEGICVVFVPHTTAGITINENADPTVKEDI 60 Query: 62 LMRLDEVYPWEHPKYRHAEGNTASHLKSITVGPSQSIIIHEGALLLGRWQGIYFCEFDGP 121 L LD++ P + ++H EGN+ +H+K+ VG S +++I G L+LG WQGIYFCEFDGP Sbjct: 61 LSALDKIIP--NISFKHLEGNSDAHIKASLVGSSVTVLIENGELVLGTWQGIYFCEFDGP 118 Query: 122 RRRQCYLKII 131 RRR+ Y+KII Sbjct: 119 RRRKVYIKII 128 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.321 0.138 0.427 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 201,164,457 Number of extensions: 7599405 Number of successful extensions: 17356 Number of sequences better than 1.0e-05: 317 Number of HSP's gapped: 17090 Number of HSP's successfully gapped: 317 Length of query: 133 Length of database: 757,237,578 Length adjustment: 95 Effective length of query: 38 Effective length of database: 499,303,173 Effective search space: 18973520574 Effective search space used: 18973520574 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 120 (50.8 bits)