BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0021.p (151 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_814911.1| autoinducer-2 production protein LuxS [Enteroco... 230 8e-60 ref|ZP_00046310.1| COG1854: LuxS protein involved in autoinducer... 190 1e-47 ref|NP_965624.1| autoinducer-2 production protein LuxS [Lactobac... 188 3e-47 ref|NP_344874.1| autoinducer-2 production protein [Streptococcus... 188 4e-47 sp|Q8KQK6|LUXS_STRGN S-ribosylhomocysteinase (Autoinducer-2 prod... 186 1e-46 >ref|NP_814911.1| autoinducer-2 production protein LuxS [Enterococcus faecalis V583] gb|AAO80981.1| autoinducer-2 production protein LuxS [Enterococcus faecalis V583] Length = 152 Score = 230 bits (586), Expect = 8e-60, Method: Compositional matrix adjust. Identities = 107/149 (71%), Positives = 127/149 (85%), Gaps = 1/149 (0%) Query: 1 MAKVESFQLDHTKVKAPYVRVAGTEKNEKGSTVQKYDLRLLQPNADALPTAAVHTLEHLL 60 MA+VESF+LDH VKAPYVR+AGTE+N + V+KYDLR LQPN DALPT A+HTLEHLL Sbjct: 1 MARVESFELDHNTVKAPYVRLAGTEQN-GDALVEKYDLRFLQPNKDALPTGALHTLEHLL 59 Query: 61 ATYLRDELEGIIDISPMGCRTGFYLIIWDEHEPADVAAALTKVLHKVLETEHVPAVSALE 120 A +RDEL+GIIDISPMGCRTGFY+I+WD+H P ++ AL VL+KV+ TE VPAVSA E Sbjct: 60 AVNMRDELKGIIDISPMGCRTGFYMIMWDQHSPQEIRDALVNVLNKVINTEVVPAVSAKE 119 Query: 121 CGNYKDHSLFSAKEYARLVLEAGISDDPF 149 CGNYKDHSLF+AKEYA++VL+ GIS DPF Sbjct: 120 CGNYKDHSLFAAKEYAKIVLDQGISLDPF 148 >ref|ZP_00046310.1| COG1854: LuxS protein involved in autoinducer AI2 synthesis [Lactobacillus gasseri] Length = 158 Score = 190 bits (482), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 85/152 (55%), Positives = 112/152 (73%), Gaps = 3/152 (1%) Query: 1 MAKVESFQLDHTKVKAPYVRVAGTEKNEKGSTVQKYDLRLLQPNADALPTAAVHTLEHLL 60 M KVESF+LDHTKVKAPYVR+ E+ +KG + +DLRL+QPN +A+PT +HT+EHLL Sbjct: 1 MGKVESFELDHTKVKAPYVRLITVEEGKKGDKISNFDLRLVQPNENAIPTGGLHTIEHLL 60 Query: 61 ATYLRDELEGIIDISPMGCRTGFYLIIWDEHEPADVAAALTKVLHKV---LETEHVPAVS 117 A LRD ++G ID SP GCRTGF+L++W DVA AL + L ++ ++ E VP + Sbjct: 61 AGLLRDRIDGYIDCSPFGCRTGFHLLVWGTPSTTDVAKALKEALEEIRDKIQWEDVPGTT 120 Query: 118 ALECGNYKDHSLFSAKEYARLVLEAGISDDPF 149 CGNY+DHSLFSAK+++R +LE GISDDPF Sbjct: 121 IKSCGNYRDHSLFSAKQWSRDILEKGISDDPF 152 >ref|NP_965624.1| autoinducer-2 production protein LuxS [Lactobacillus johnsonii NCC 533] gb|AAS09590.1| autoinducer-2 production protein LuxS [Lactobacillus johnsonii NCC 533] Length = 158 Score = 188 bits (478), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 85/152 (55%), Positives = 111/152 (73%), Gaps = 3/152 (1%) Query: 1 MAKVESFQLDHTKVKAPYVRVAGTEKNEKGSTVQKYDLRLLQPNADALPTAAVHTLEHLL 60 M KVESF+LDHTKVKAPYVR+ E+ +KG + +DLRL+QPN +A+PT +HT+EHLL Sbjct: 1 MGKVESFELDHTKVKAPYVRLITVEEGQKGDKISNFDLRLVQPNENAIPTGGLHTIEHLL 60 Query: 61 ATYLRDELEGIIDISPMGCRTGFYLIIWDEHEPADVAAALTKVLHKVLET---EHVPAVS 117 A LRD ++G ID SP GCRTGF+L++W DVA AL + L ++ + E VP + Sbjct: 61 AGLLRDRIDGYIDCSPFGCRTGFHLLVWGTPSTTDVAKALKESLEEIRDNITWEDVPGTT 120 Query: 118 ALECGNYKDHSLFSAKEYARLVLEAGISDDPF 149 CGNY+DHSLFSAK+++R +LE GISDDPF Sbjct: 121 IESCGNYRDHSLFSAKQWSRDILEKGISDDPF 152 >ref|NP_344874.1| autoinducer-2 production protein [Streptococcus pneumoniae TIGR4] ref|NP_357902.1| Autoinducer-2 production protein [Streptococcus pneumoniae R6] sp|Q97SJ9|LUXS_STRPN S-ribosylhomocysteinase (Autoinducer-2 production protein LuxS) (AI-2 synthesis protein) pir||A95040 autoinducer-2 production protein [imported] - Streptococcus pneumoniae (strain TIGR4) pir||D97910 autoinducer-2 production protein [imported] - Streptococcus pneumoniae (strain R6) gb|AAK74514.1| autoinducer-2 production protein [Streptococcus pneumoniae TIGR4] gb|AAK99112.1| Autoinducer-2 production protein [Streptococcus pneumoniae R6] Length = 160 Score = 188 bits (477), Expect = 4e-47, Method: Compositional matrix adjust. Identities = 85/149 (57%), Positives = 109/149 (73%), Gaps = 3/149 (2%) Query: 4 VESFQLDHTKVKAPYVRVAGTEKNEKGSTVQKYDLRLLQPNADALPTAAVHTLEHLLATY 63 VESF+LDHT VKAPYVR+ G E KG + YD+RL+QPN D++PTA +HT+EHLLA Sbjct: 7 VESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLVQPNEDSIPTAGLHTIEHLLAKL 66 Query: 64 LRDELEGIIDISPMGCRTGFYLIIWDEHEPADVAAALTKVLHKVLET---EHVPAVSALE 120 +R ++G+ID SP GCRTGF++I+W H A +AA + L ++ ET E VP + Sbjct: 67 IRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAVIKDSLKEIAETTTWEDVPGTTIES 126 Query: 121 CGNYKDHSLFSAKEYARLVLEAGISDDPF 149 CGNYKDHSLFSAKE+A+L+LE GISDD F Sbjct: 127 CGNYKDHSLFSAKEWAKLILEQGISDDAF 155 >sp|Q8KQK6|LUXS_STRGN S-ribosylhomocysteinase (Autoinducer-2 production protein LuxS) (AI-2 synthesis protein) gb|AAL87464.1| autoinducer-2 production protein [Streptococcus gordonii] Length = 160 Score = 186 bits (473), Expect = 1e-46, Method: Compositional matrix adjust. Identities = 81/149 (54%), Positives = 110/149 (73%), Gaps = 3/149 (2%) Query: 4 VESFQLDHTKVKAPYVRVAGTEKNEKGSTVQKYDLRLLQPNADALPTAAVHTLEHLLATY 63 VESF+LDHT VKAPYVR+ G E KG + +D+RL+QPN D++PTA +HT+EHLLA Sbjct: 7 VESFELDHTIVKAPYVRLIGEESGPKGDVISNFDIRLVQPNEDSIPTAGLHTIEHLLAML 66 Query: 64 LRDELEGIIDISPMGCRTGFYLIIWDEHEPADVAAALTKVLHKVLET---EHVPAVSALE 120 +R ++G+ID SP GCRTGF++I+W H P+++A + L ++ ET E VP + Sbjct: 67 IRKRIDGMIDCSPFGCRTGFHMIMWGRHTPSEIAQVIKSCLEEIAETTTWEDVPGTTIES 126 Query: 121 CGNYKDHSLFSAKEYARLVLEAGISDDPF 149 CGNYKDHSLFSAKE+A+L+L+ GISD+ F Sbjct: 127 CGNYKDHSLFSAKEWAKLILKQGISDNAF 155 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.133 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 217,272,589 Number of extensions: 8100003 Number of successful extensions: 16721 Number of sequences better than 1.0e-05: 86 Number of HSP's gapped: 16605 Number of HSP's successfully gapped: 86 Length of query: 151 Length of database: 757,237,578 Length adjustment: 110 Effective length of query: 41 Effective length of database: 458,576,688 Effective search space: 18801644208 Effective search space used: 18801644208 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 120 (50.8 bits)