BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0307.0016.p (382 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_471183.1| similar to glycerol dehydrogenase [Listeria inn... 393 e-108 ref|NP_465262.1| similar to glycerol dehydrogenase [Listeria mon... 392 e-107 ref|NP_814633.1| glycerol dehydrogenase, putative [Enterococcus ... 326 6e-88 ref|NP_694250.1| glycerol dehydrogenase [Oceanobacillus iheyensi... 245 1e-63 ref|NP_816748.1| alcohol dehydrogenase, iron-containing [Enteroc... 231 3e-59 >ref|NP_471183.1| similar to glycerol dehydrogenase [Listeria innocua] pir||AG1663 glycerol dehydrogenase homolog lin1848 [imported] - Listeria innocua (strain Clip11262) emb|CAC97079.1| lin1848 [Listeria innocua] Length = 368 Score = 393 bits (1010), Expect = e-108, Method: Compositional matrix adjust. Identities = 189/345 (54%), Positives = 241/345 (69%) Query: 10 VEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKETEV 69 + ++IVR APQE+ C+ G+W +L HL+ SW A +P L Sbjct: 2 LNKELIVRGAPQEYFCQVGAWDTLPTHLERRGLKNVLVVRGNASWEVAKKKFPVLSNVTS 61 Query: 70 HYHVYSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPTLAA 129 + VY+G +Y ERD + + ++A+IAVGGGKI D+ K+AAA L LP IILPTLA+ Sbjct: 62 TFEVYNGGSTYEERDRLVAIMKTNNMDAIIAVGGGKIADVCKAAAAVLRLPVIILPTLAS 121 Query: 130 TCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKWYEA 189 TCAA++ LSVMYD GA IR++VF SNAL+L+DP +I SP ELL AGIGDTLAKWYEA Sbjct: 122 TCAAYTPLSVMYDVEGAMIRYDVFASSNALLLIDPEMILDSPKELLVAGIGDTLAKWYEA 181 Query: 190 DVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIMVAG 249 DVII+ L VE+E+A AA+ CR+NLL++S AL A+ ELNEA + ET I+V G Sbjct: 182 DVIIKSLPTKSVEIEIAHIAAKMCRDNLLQFSEEALNAMDKQELNEAFVKIIETNILVGG 241 Query: 250 LVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGLLPF 309 +VGGFG+DYGR AGAHS+HDALT +PETH LLHG+KVAYG+LVQL +E W EID LLPF Sbjct: 242 MVGGFGDDYGRCAGAHSIHDALTMVPETHHLLHGNKVAYGILVQLVIENRWNEIDQLLPF 301 Query: 310 YEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y +LGLP+SL MGL LT E ++ + +RA P +IHMMPG +T Sbjct: 302 YAKLGLPTSLYDMGLATLTEETIVAVSKRATEPHETIHMMPGEMT 346 >ref|NP_465262.1| similar to glycerol dehydrogenase [Listeria monocytogenes EGD-e] pir||AI1291 glycerol dehydrogenase homolog lmo1737 [imported] - Listeria monocytogenes (strain EGD-e) emb|CAC99815.1| lmo1737 [Listeria monocytogenes] Length = 368 Score = 392 bits (1006), Expect = e-107, Method: Compositional matrix adjust. Identities = 191/345 (55%), Positives = 240/345 (69%) Query: 10 VEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKETEV 69 + ++IVR APQE++C+ G+W +L HL+ SW A +P L Sbjct: 2 LNKELIVRGAPQEYLCQVGAWDTLPTHLERRGLKNVLVVRGNASWEVAKKKFPVLSTVTS 61 Query: 70 HYHVYSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPTLAA 129 + VY+G +Y ERD + ++++A+IAVGGGKI D+ K+AAA L LP IILPTLA+ Sbjct: 62 TFEVYNGGSTYEERDRLVAIMETNKMDAIIAVGGGKIADVCKAAAAVLRLPVIILPTLAS 121 Query: 130 TCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKWYEA 189 TCAA++ LSVMYDE GA IR++VF SNAL+L+DP +I SP ELL AGIGDTLAKWYEA Sbjct: 122 TCAAYTPLSVMYDEEGAMIRYDVFASSNALLLIDPEMILDSPKELLIAGIGDTLAKWYEA 181 Query: 190 DVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIMVAG 249 DVII L VE+E+A AA+ CR+NLL+YS ALAA+ LNEA + ET I+V G Sbjct: 182 DVIINSLPTKSVEIEIAHFAAKMCRDNLLQYSGEALAAMDKQVLNEAFVKIIETNILVGG 241 Query: 250 LVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGLLPF 309 +VGGFG+DYGR AGAHS+HDALT +PETH LLHG+KVAYG+LVQL +E W EI+ LLPF Sbjct: 242 MVGGFGDDYGRCAGAHSIHDALTMVPETHHLLHGNKVAYGILVQLVIENRWDEIERLLPF 301 Query: 310 YEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y ELGLP SL MGL L E L+ + RA P +IHMMPG +T Sbjct: 302 YSELGLPMSLYDMGLATLAEETLVDVSRRATEPHETIHMMPGEMT 346 >ref|NP_814633.1| glycerol dehydrogenase, putative [Enterococcus faecalis V583] gb|AAO80703.1| glycerol dehydrogenase, putative [Enterococcus faecalis V583] Length = 362 Score = 326 bits (835), Expect = 6e-88, Method: Compositional matrix adjust. Identities = 166/345 (48%), Positives = 222/345 (64%), Gaps = 1/345 (0%) Query: 10 VEPQIIVRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKETEV 69 +E +IVR PQE++C GSW LE L SW AA PY+P ++ Sbjct: 1 MEQSLIVRGTPQEYLCEIGSWELLEGQLLCRNIHRVFILHGTESWQAAKPYFPTFQKVTA 60 Query: 70 HYHVYSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPTLAA 129 + Y G C+ + + ++ LEA++AVGGGK+ DL K+ A + LP IILPTLAA Sbjct: 61 VFENYGGVCTDQRVQELEAQVLDNHLEAIVAVGGGKVADLGKALAHKMALPVIILPTLAA 120 Query: 130 TCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKWYEA 189 TCA + LSVMY E GA R++VFP++NALVL++P V+ SP L+ AGIGDTLAKWYEA Sbjct: 121 TCAPCTPLSVMYREDGAMERYDVFPQANALVLVEPRVLLHSPRSLMVAGIGDTLAKWYEA 180 Query: 190 DVIIRHLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIMVAG 249 D II L+ + ++++ AA KCR+ LL S AL A+ +LN++ ET +++ G Sbjct: 181 DAIISQLDVQVLPIQVSHFAAEKCRDILLNESINALKAMEEQQLNQSFIDVIETNLIIGG 240 Query: 250 LVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVAYGVLVQLALEGNWPEIDGLLPF 309 +VGGFG+DYGRTAGAHS+HDALT +P +H LHG+KVAYGV VQLA+E W EI L+PF Sbjct: 241 MVGGFGDDYGRTAGAHSIHDALTLLPASHRQLHGNKVAYGVFVQLAIEEKWQEIAELIPF 300 Query: 310 YEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVIT 354 Y +LGLP SL+ M + LT E ++ ERA + +IH M IT Sbjct: 301 YHQLGLPISLKEMDMD-LTEAEYQEVAERACIEGETIHYMKQKIT 344 >ref|NP_694250.1| glycerol dehydrogenase [Oceanobacillus iheyensis HTE831] dbj|BAC15284.1| glycerol dehydrogenase [Oceanobacillus iheyensis HTE831] Length = 377 Score = 245 bits (626), Expect = 1e-63, Method: Compositional matrix adjust. Identities = 135/307 (43%), Positives = 189/307 (61%), Gaps = 5/307 (1%) Query: 53 SWTAAAPYWPEL--KETEVHYHVYSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLV 110 SW AA PY P + K+ E + G + + D IA +H ++AVI +GGG D+ Sbjct: 43 SWQAAKPYLPFVLVKQVEANIIFIDGHTTLEKADKIADQLHQHAIDAVIGIGGGTALDIT 102 Query: 111 KSAAATLNLPAIILPTLAATCAAWSSLSVMYDERGAFIRFEVFPRSNALVLLDPVVIAAS 170 K+AAA + ++++PT+AATCAAW+ LSV YD GAF + FP + LVL++P +IA + Sbjct: 103 KAAAAKAEVKSVLIPTIAATCAAWTPLSVFYDSNGAFTHYTYFPLATTLVLVEPAIIAHA 162 Query: 171 PPELLAAGIGDTLAKWYEADVIIRHL---EAPPVEVELAWDAARKCRENLLRYSSAALAA 227 P E L AGIGDTLAK+YEAD +I E PV V+++ +A CR+ LL AAL A Sbjct: 163 PIEYLRAGIGDTLAKFYEADALIESFYPNEEVPVPVKVSQFSASICRDVLLEDGKAALDA 222 Query: 228 VRSGELNEALTRTAETIIMVAGLVGGFGEDYGRTAGAHSVHDALTAIPETHGLLHGSKVA 287 VR ++ AL R E II+ G+VGGFG+ GR AGAHS+H+ LT E LHG VA Sbjct: 223 VRDKQVTPALIRVIEAIIITGGMVGGFGDKAGRIAGAHSLHNGLTEASEAQHFLHGELVA 282 Query: 288 YGVLVQLALEGNWPEIDGLLPFYEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIH 347 YG+L QLA E E+ LL FY++ LPSSL+ +G+ + + ++ ++A +P+ SIH Sbjct: 283 YGILGQLAFEQKEEELLRLLDFYDQWQLPSSLQEIGIDITNTDLVERIIQKAILPQESIH 342 Query: 348 MMPGVIT 354 M +T Sbjct: 343 FMNREVT 349 >ref|NP_816748.1| alcohol dehydrogenase, iron-containing [Enterococcus faecalis V583] gb|AAO82818.1| alcohol dehydrogenase, iron-containing [Enterococcus faecalis V583] Length = 368 Score = 231 bits (588), Expect = 3e-59, Method: Compositional matrix adjust. Identities = 142/366 (38%), Positives = 207/366 (56%), Gaps = 11/366 (3%) Query: 16 VRAAPQEFICRPGSWGSLEQHLQXXXXXXXXXXXXXXSWTAAAPYWPELKETEVH--YHV 73 V+ PQ + G+ S+ + S+ A P+ P L ++E YH Sbjct: 7 VKVGPQFYRYHEGALASVPSLFKEYHAQRILVVHGTVSFEKAQPFLPFLADSEYQFFYHT 66 Query: 74 YSGECSYRERDAIAGYASEHQLEAVIAVGGGKITDLVKSAAATLNLPAIILPTLAATCAA 133 Y+GECSY + I+ EHQ++ ++ VGGGK+ DLV +A NL ++PTLA+ CA Sbjct: 67 YTGECSYFGAEQISQQIKEHQIDFLLGVGGGKLADLVGYSAHLNNLNFGLVPTLASNCAP 126 Query: 134 WSSLSVMYDERGAFI-RFEVFPRSNALVLLDPVVIAASPPELLAAGIGDTLAKWYEADVI 192 W+ L+VMY E GA + E F R A ++ DP ++ +P + AG+ DTLAKWYE++ I Sbjct: 127 WTPLAVMYQENGAAEGKTEHFFRQAAFLITDPKLLLDAPRDYFVAGLADTLAKWYESETI 186 Query: 193 IR--HLEAPPVEVELAWDAARKCRENLLRYSSAALAAVRSGELNEALTRTAETIIMVAGL 250 +R HL+ P ++LA A+ +E ++R S AALAA+ G+L +E + V+GL Sbjct: 187 LRQAHLQGEPF-LQLAGATAKLSQEAIMRDSKAALAAMDEGKLTPEFVHLSEIVFAVSGL 245 Query: 251 VGGFGEDYGRTAGAHSVHDALTA-IPETHGLLHGSKVAYGVLVQLALEGNWPEIDGLLPF 309 VGGFG+ Y R A AH++HDA++ +P++H LHG KVAYG+ QLALE W ID L+PF Sbjct: 246 VGGFGDKYARNAAAHAMHDAMSKFLPKSHDYLHGEKVAYGIFYQLALEKRWAIIDALIPF 305 Query: 310 YEELGLPSSLRAMGLGHLTREELLKLGERAAVPEASIHMMPGVITXXXXXXXXXXXXXYI 369 Y+EL LP SLR MGL + E +L + + +H++P I+ YI Sbjct: 306 YQELNLPMSLRQMGL-YPKEEAVLDAMVQFIDSKEKVHLIPIEISEERLRQGIKELEKYI 364 Query: 370 TAGQNQ 375 QNQ Sbjct: 365 ---QNQ 367 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 515,801,493 Number of extensions: 19385145 Number of successful extensions: 50470 Number of sequences better than 1.0e-05: 140 Number of HSP's gapped: 50281 Number of HSP's successfully gapped: 140 Length of query: 382 Length of database: 757,237,578 Length adjustment: 127 Effective length of query: 255 Effective length of database: 412,420,005 Effective search space: 105167101275 Effective search space used: 105167101275 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 127 (53.5 bits)