BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0025.p (341 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_229030.1| conserved hypothetical protein [Thermotoga mari... 436 e-121 ref|NP_809946.1| conserved hypothetical protein [Bacteroides the... 337 3e-91 ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium t... 204 3e-51 ref|NP_634795.1| conserved protein [Methanosarcina mazei Goe1] >... 191 1e-47 ref|NP_617288.1| conserved hypothetical protein [Methanosarcina ... 187 4e-46 >ref|NP_229030.1| conserved hypothetical protein [Thermotoga maritima] pir||B72278 conserved hypothetical protein - Thermotoga maritima (strain MSB8) gb|AAD36300.1| conserved hypothetical protein [Thermotoga maritima] Length = 326 Score = 436 bits (1121), Expect = e-121, Method: Compositional matrix adjust. Identities = 200/317 (63%), Positives = 251/317 (79%) Query: 9 SQNIPNMPWQDRPAGNDNPVWRHSDNPVIKRNPAKGVARIFNSAVVAYEGSFLGVFRVED 68 ++ IPN+PW++RP G PVWR+S NP+I RNP AR+FNSAVV Y G F+GVFR++ Sbjct: 5 TEKIPNIPWEERPEGYTGPVWRYSKNPIIGRNPVPKGARVFNSAVVPYNGEFVGVFRIDH 64 Query: 69 NTTRPHLRMGHSVDGLDWKIDDEPIQFVDEAGEPYMPRYAYDPRLVKVEDTYYIIWCTDF 128 TRP L G S DG++W+I+ E IQ+VD GEP+ P YAYDPR+VK+EDTYYI +CTD Sbjct: 65 KNTRPFLHFGRSKDGINWEIEPEEIQWVDVNGEPFQPSYAYDPRVVKIEDTYYITFCTDD 124 Query: 129 YGAAIGVAKTRDFKTFISLENPFLPFNRNGVLFPRKINGNFVMLSRPSDSGHTPFGDVFL 188 +G IGV T+DFKTF+ L N ++PFNRNGVLFPRKING +VML+RPSD+GHTPFGD+FL Sbjct: 125 HGPTIGVGMTKDFKTFVRLPNAYVPFNRNGVLFPRKINGKYVMLNRPSDNGHTPFGDIFL 184 Query: 189 SESPDFVYWGKHRHVMTKGGQGWWQSTKIGGGPAPIETTEGWLMFYHGVTGTCNGLVYSM 248 SESPD ++WG HR V+ + WW++ KIG GP PIET+EGWL+ YHGVT TCNG VYS Sbjct: 185 SESPDMIHWGNHRFVLGRSSYNWWENLKIGAGPYPIETSEGWLLIYHGVTLTCNGYVYSF 244 Query: 249 GAVILDKDEPSKVKYRSKNFVLTPEEWYEERGFVNNVLFPCATLNDAETGRIAIYYGAAD 308 GA +LD D+PSKV YRS+ ++LTPEE YE GFV NV+FPCA L DA+TGR+AIYYGAAD Sbjct: 245 GAALLDLDDPSKVLYRSRYYLLTPEEEYETVGFVPNVVFPCAALCDADTGRVAIYYGAAD 304 Query: 309 TYVGLAYTTVEEIVNYV 325 T+V LA+ ++EIV++V Sbjct: 305 THVALAFGYIDEIVDFV 321 >ref|NP_809946.1| conserved hypothetical protein [Bacteroides thetaiotaomicron VPI-5482] gb|AAO76140.1| conserved hypothetical protein [Bacteroides thetaiotaomicron VPI-5482] Length = 322 Score = 337 bits (863), Expect = 3e-91, Method: Compositional matrix adjust. Identities = 163/320 (50%), Positives = 212/320 (66%), Gaps = 4/320 (1%) Query: 11 NIPNMPWQDRPAGNDNPVWRHSDNPVIKRNPAKGVARIFNSAVVAYEGSFLGVFRVEDNT 70 N +PW++RP G + +WR+S NPVI R IFNSAVV ++ F GVFR ++ Sbjct: 2 NKIQIPWEERPVGCTDVMWRYSQNPVIGRYHIPSSNSIFNSAVVPFKDGFAGVFRCDNKA 61 Query: 71 TRPHLRMGHSVDGLDWKIDDEPIQFVDEAGEPYMPRYAYDPRLVKVEDTYYIIWCTDFYG 130 + ++ G S DG+ W I EPIQF E Y YDPR+ +ED Y++ WC ++G Sbjct: 62 VQMNIFTGFSKDGIHWDISHEPIQFKAGNTEMIESEYKYDPRVTWIEDRYWVTWCNGYHG 121 Query: 131 AAIGVAKTRDFKTFISLENPFLPFNRNGVLFPRKINGNFVMLSRPSDSGHTPFGDVFLSE 190 IG+A T DF F EN FLPFNRNGVLFP+KI+G + MLSRPSD+GHTPFGD+++S Sbjct: 122 PTIGIAYTFDFVDFFQCENAFLPFNRNGVLFPQKIDGKYAMLSRPSDNGHTPFGDIYISY 181 Query: 191 SPDFVYWGKHRHVM--TKGGQGWWQSTKIGGGPAPIETTEGWLMFYHGVTGTCNGLVYSM 248 SPD YWG+HR VM T + WQ TKIG G P T EGWL+FYHGV TCNG Y+M Sbjct: 182 SPDMKYWGEHRCVMKVTPFPESAWQCTKIGAGSVPFLTDEGWLLFYHGVITTCNGFRYAM 241 Query: 249 GAVILDKDEPSKVKYRSKNFVLTPEEWYEERGFVNNVLFPCATLNDAETGRIAIYYGAAD 308 G+ ILDKD P KV YR++ +++ P YE +G V NV+FPCA L D E R+A+YYGAAD Sbjct: 242 GSAILDKDHPEKVLYRTREYLIGPAAPYELQGDVPNVVFPCAALQDGE--RVAVYYGAAD 299 Query: 309 TYVGLAYTTVEEIVNYVIAT 328 T VG+A+ ++EI+++ T Sbjct: 300 TVVGMAFGYIQEIIDFTKRT 319 >ref|ZP_00059726.1| COG2152: Predicted glycosylase [Clostridium thermocellum ATCC 27405] Length = 334 Score = 204 bits (519), Expect = 3e-51, Method: Compositional matrix adjust. Identities = 111/310 (35%), Positives = 165/310 (53%), Gaps = 14/310 (4%) Query: 30 RHSDNPVIKRNPAKGVAR----------IFNSAVVAYEGSFLGVFRVEDNTTRPHLRMGH 79 RHS + KR P + +FN A Y G L + RVED HL Sbjct: 18 RHSYKELFKRFPGNPILSAKNWPYAANTVFNPAATMYNGKVLLLIRVEDRRGFSHLTKAI 77 Query: 80 SVDGL-DWKIDDEPIQFVDEAGEPYMPRYAYDPRLVKVEDTYYIIWCTDFY---GAAIGV 135 S DG+ +W IDD+P + P DPR+ +E+ Y G + + Sbjct: 78 SDDGISNWIIDDKPTLEAEPEKFPEEEWGVEDPRITWIEELGKFAVVYTAYSKGGPLVSL 137 Query: 136 AKTRDFKTFISLENPFLPFNRNGVLFPRKINGNFVMLSRPSDSGHTPFGDVFLSESPDFV 195 A T DF+ F L P +++ LFPR+ING +V++ RP H P +++S S D Sbjct: 138 ALTEDFENFEKLGAIMPPEDKDAALFPRRINGKWVLIHRPISIHHGPGAHIWISRSDDLK 197 Query: 196 YWGKHRHVMTKGGQGWWQSTKIGGGPAPIETTEGWLMFYHGVTGTCNGLVYSMGAVILDK 255 YWG H+ ++ GWW + K+G P+ET +GWL+ YHGV T +G +Y +G +LD Sbjct: 198 YWGDHQILIRARKGGWWDANKVGLNCPPLETPDGWLILYHGVRQTASGSIYRLGLALLDL 257 Query: 256 DEPSKVKYRSKNFVLTPEEWYEERGFVNNVLFPCATLNDAETGRIAIYYGAADTYVGLAY 315 + PSKV RS +V P+E+YE G V++V+FPC + + +TG I IYYGAADT + +A Sbjct: 258 ENPSKVLRRSDEWVFGPQEFYEREGDVDDVVFPCGWVYNEKTGEIKIYYGAADTCIAMAT 317 Query: 316 TTVEEIVNYV 325 T + ++++Y+ Sbjct: 318 TNISDLLDYI 327 >ref|NP_634795.1| conserved protein [Methanosarcina mazei Goe1] gb|AAM32467.1| conserved protein [Methanosarcina mazei Goe1] Length = 339 Score = 191 bits (486), Expect = 1e-47, Method: Compositional matrix adjust. Identities = 115/322 (35%), Positives = 172/322 (53%), Gaps = 11/322 (3%) Query: 15 MPWQDRPAGNDNPVWRHSDNPVIKRNPAKGVAR-IFNSAVVAYEGSFLGVFRVEDNTTRP 73 M W+DR R++ NP++ + A +FN A +G L + RVED+ Sbjct: 1 MTWKDR----GELFVRYNRNPILTVDDLSYRANSVFNPAAAIVDGKTLLLLRVEDHRGFS 56 Query: 74 HLRMGHSVDGLD-WKIDDEPIQFVDEAGEPYMPRYAYDPRLVKVED-TYYIIWCTDFY-- 129 H + S +G+D W+ID +P F + + P DPR+ +++ + I T F Sbjct: 57 HFTVARSKNGIDDWEIDGKPTFFPEPSKYPEEIYGIEDPRITYMDELKMWAIVYTAFSDS 116 Query: 130 GAAIGVAKTRDFKTFISLENPFLPFNRNGVLFPRKINGNFVMLSRPSDSGHTPFGDVFLS 189 G +A T DF+TF + P N++ +FP NG + ML RP+ +GHT ++++S Sbjct: 117 GPLPALALTEDFQTFERIGPVAPPDNKDAAIFPVSFNGKWAMLHRPAATGHTLKSNIWIS 176 Query: 190 ESPDFVYWGKHRHVMTKGGQGWWQSTKIGGGPAPIETTEGWLMFYHGVTGTCNGLVYSMG 249 SPD WG H+ + GWW + KIG P P+ T EGWL+ YHGV T + + Y +G Sbjct: 177 FSPDMKAWGWHQVLFYAREGGWWDAYKIGLSPQPMYTPEGWLIMYHGVRQTTSKISYRLG 236 Query: 250 AVILDKDEPSKVKYRSKNFVLTPEEWYEERGFVNNVLFPCATLNDAETGRIAIYYGAADT 309 +LD +P KV RS+ +V P EWYE G VN+V+FPC + G + IYYG ADT Sbjct: 237 LALLDLKDPRKVLRRSEGWVFGPHEWYERSGDVNDVVFPCGWV--VVDGELRIYYGGADT 294 Query: 310 YVGLAYTTVEEIVNYVIATHEE 331 V LA +++I+ Y++ EE Sbjct: 295 SVSLASAKMDDIMKYILECPEE 316 >ref|NP_617288.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] gb|AAM05768.1| conserved hypothetical protein [Methanosarcina acetivorans str. C2A] Length = 335 Score = 187 bits (474), Expect = 4e-46, Method: Compositional matrix adjust. Identities = 109/308 (35%), Positives = 163/308 (52%), Gaps = 9/308 (2%) Query: 30 RHSDNPVIK-RNPAKGVARIFNSAVVAYEGSFLGVFRVEDNTTRPHLRMGHSVDGLD-WK 87 RH NP++ + +FN A E L + RVED+ H + S +G+D W+ Sbjct: 12 RHKKNPILTVEDWPYQANSVFNPAAAMVESKTLLLVRVEDHRGFSHFTVARSNNGIDGWE 71 Query: 88 IDDEPIQFVDEAGEPYMPRYAYDPRLVKVEDT-YYIIWCTDFY--GAAIGVAKTRDFKTF 144 ID EP D P DPR+ +++ + + T F G +A T+DF+ F Sbjct: 72 IDTEPTLTPDTVNYPEEIYGIEDPRITYIDEMGRWAVAYTAFSDSGPLTSLALTKDFRNF 131 Query: 145 ISLENPFLPFNRNGVLFPRKINGNFVMLSRPSDSGHTPFGDVFLSESPDFVYWGKHRHVM 204 + P N++ LFP + NG + ML RP S ++++S SPD +WG H+ ++ Sbjct: 132 ERIGATMPPENKDSALFPVRFNGKWAMLHRPVSSMSGAKANIWISFSPDMRHWGDHQVLL 191 Query: 205 TKGGQGWWQSTKIGGGPAPIETTEGWLMFYHGVTGTCNGLVYSMGAVILDKDEPSKVKYR 264 GWW + KIG P PI T +GWL+ YHGV T + L Y +G +LD ++P KV +R Sbjct: 192 YAREGGWWDARKIGLSPQPIRTYDGWLIMYHGVRPTTSKLSYRLGMALLDLEDPRKVLHR 251 Query: 265 SKNFVLTPEEWYEERGFVNNVLFPCA-TLNDAETGRIAIYYGAADTYVGLAYTTVEEIVN 323 S+ +V P E YE G VN+V+FPC L+D + I +YYG+ADT V LA + +++ Sbjct: 252 SEGWVFGPRELYERSGDVNDVVFPCGWVLHDDQ---IRLYYGSADTSVSLASAKLHDVLE 308 Query: 324 YVIATHEE 331 Y+ EE Sbjct: 309 YIHECPEE 316 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.139 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 582,340,929 Number of extensions: 26819246 Number of successful extensions: 45770 Number of sequences better than 1.0e-05: 32 Number of HSP's gapped: 45684 Number of HSP's successfully gapped: 32 Length of query: 341 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 215 Effective length of database: 415,135,104 Effective search space: 89254047360 Effective search space used: 89254047360 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 126 (53.1 bits)