BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0305.0019.p (276 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00100010.1| COG4753: Response regulator containing CheY-l... 140 2e-32 ref|NP_244546.1| two-component response regulator [Bacillus halo... 134 3e-30 ref|NP_358172.1| Response regulator [Streptococcus pneumoniae R6... 126 4e-28 ref|NP_345166.1| DNA-binding response regulator [Streptococcus p... 126 4e-28 ref|NP_665006.1| putative two-component response regulator [Stre... 125 1e-27 >ref|ZP_00100010.1| COG4753: Response regulator containing CheY-like receiver domain and AraC-type DNA-binding domain [Desulfitobacterium hafniense] Length = 289 Score = 140 bits (354), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 85/273 (31%), Positives = 145/273 (53%), Gaps = 15/273 (5%) Query: 11 IICSFNETGGDLLVIRVM----IADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNH 66 + S GG + V + M I DDE +IR+G++++ + + + + +G A + Sbjct: 14 VALSIQAVGGKVRVQKTMYKLLIVDDEPIIRQGIKQLINFQEQSISEVYEAEDGALALDI 73 Query: 67 LRELSQDDIDLLITDIKMPRMDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVL 126 ++E D +++ DI MP +DG L E++ ++ +AV++G+ FEYA A++ GV Sbjct: 74 VKEKRPD---IVLADINMPNLDGLNLAREIKAVDPEIRVAVITGYDYFEYAVTALKAGVD 130 Query: 127 DYLLKPIEKAQLYDL-------LKSVEENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKS 179 DYLLKP+ ++ + +L LK E ++ A ++ H EG HY + Sbjct: 131 DYLLKPVSRSDILELLSKLVNDLKEKTEQRRAMDSLASLKQLEHGGNEGHHHYREMILQV 190 Query: 180 ILEKEYGQNFELERLAETVGMNASYISRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDL 239 + F L RLAE + +++ Y+S +FK G +YL +R+ ++K LL+ Sbjct: 191 MNHSLSDPAFSLGRLAEAINLSSGYLSTVFKQNFGIPFQEYLTTMRLERSKILLL-VTSY 249 Query: 240 KNYEIAERVGYSDPVYFNKLFKKMCGMTPKDYK 272 K YEIAE+VG+ DP YF+ FKK G++P Y+ Sbjct: 250 KIYEIAEKVGFDDPSYFSTCFKKRYGLSPNSYR 282 >ref|NP_244546.1| two-component response regulator [Bacillus halodurans] pir||G84109 two-component response regulator BH3679 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07398.1| two-component response regulator [Bacillus halodurans] Length = 257 Score = 134 bits (336), Expect = 3e-30, Method: Compositional matrix adjust. Identities = 86/252 (34%), Positives = 139/252 (55%), Gaps = 10/252 (3%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDIDLLITDIKMPR 86 V+I DDE RRGL++ ++ + + + EA + L+++ IDL+ITDI+MP Sbjct: 6 VLIVDDEPRSRRGLKRTLEAWSPKLLNVQTAASAKEA---ITRLNEETIDLIITDIRMPE 62 Query: 87 MDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSVE 146 M G L++ ++ + V+S +SEFEYA A+ GV YLLKP++K +L + ++S Sbjct: 63 MSGLHLVKALQEQNPKPVCIVISAYSEFEYAHTALELGVRHYLLKPVKKEKLIEAVESAL 122 Query: 147 ENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSI------LEKEYGQNFELERLAETVGM 200 + KK + + E+ + + E + + SI ++ Q L+ +A++V + Sbjct: 123 QMKKEQDRFGQVEKVWDEKIDAIEKERLSSSDSIHEALHYIDAHLDQKIGLKDVAKSVHL 182 Query: 201 NASYISRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLF 260 N SY+S LFK KT T ++YL R+ AK LL+ D +IAE+ GY YF K+F Sbjct: 183 NPSYLSALFKEKTNMTFSEYLTRKRLQHAKHLLVST-DRPISDIAEKCGYQTAKYFIKIF 241 Query: 261 KKMCGMTPKDYK 272 K+ GMTP Y+ Sbjct: 242 KEHEGMTPTQYR 253 >ref|NP_358172.1| Response regulator [Streptococcus pneumoniae R6] pir||B97944 response regulator [imported] - Streptococcus pneumoniae (strain R6) gb|AAK99382.1| Response regulator [Streptococcus pneumoniae R6] Length = 251 Score = 126 bits (317), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 142/247 (57%), Gaps = 12/247 (4%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDI-DLLITDIKMP 85 ++I +DE ++R+GL K+ + ++++IG NG +AW EL Q + D+++TDI MP Sbjct: 11 ILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAW----ELIQKQVPDIILTDINMP 66 Query: 86 RMDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSV 145 ++G +L VR Q+ + L+G+ +F+YA A++ GV DYLLKP + + ++L + Sbjct: 67 HLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKI 126 Query: 146 EENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSILEKEYGQNFELERLAETVGMNASYI 205 ++ + ++ EG + ++ +S L F L+ LA +G + +Y+ Sbjct: 127 KQKLDKEEKEEQLQDLLTNRFEGN---MAQKIQSHLADS---QFSLKSLASDLGFSPTYL 180 Query: 206 SRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLFKKMCG 265 S L K + G DYL+ R+ +AK LL+ LK YEIAE+VG+ D YF + FK++ G Sbjct: 181 SSLIKKELGLPFQDYLVRERVKQAKLLLLTTD-LKIYEIAEKVGFEDMNYFTQRFKQIAG 239 Query: 266 MTPKDYK 272 +TP+ +K Sbjct: 240 VTPRQFK 246 >ref|NP_345166.1| DNA-binding response regulator [Streptococcus pneumoniae TIGR4] pir||E95076 DNA-binding response regulator [imported] - Streptococcus pneumoniae (strain TIGR4) emb|CAB54580.1| response regulator [Streptococcus pneumoniae] gb|AAF31452.1| putative response regulator [Streptococcus pneumoniae] gb|AAK74806.1| DNA-binding response regulator [Streptococcus pneumoniae TIGR4] Length = 245 Score = 126 bits (317), Expect = 4e-28, Method: Compositional matrix adjust. Identities = 82/247 (33%), Positives = 142/247 (57%), Gaps = 12/247 (4%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDI-DLLITDIKMP 85 ++I +DE ++R+GL K+ + ++++IG NG +AW EL Q + D+++TDI MP Sbjct: 5 ILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAW----ELIQKQVPDIILTDINMP 60 Query: 86 RMDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSV 145 ++G +L VR Q+ + L+G+ +F+YA A++ GV DYLLKP + + ++L + Sbjct: 61 HLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDDYLLKPFSRQDIEEMLGKI 120 Query: 146 EENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSILEKEYGQNFELERLAETVGMNASYI 205 ++ + ++ EG + ++ +S L F L+ LA +G + +Y+ Sbjct: 121 KQKLDKEEKEEQLQDLLTNRFEGN---MAQKIQSHLADS---QFSLKSLASDLGFSPTYL 174 Query: 206 SRLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLFKKMCG 265 S L K + G DYL+ R+ +AK LL+ LK YEIAE+VG+ D YF + FK++ G Sbjct: 175 SSLIKKELGLPFQDYLVRERVKQAKLLLLTTD-LKIYEIAEKVGFEDMNYFTQRFKQIAG 233 Query: 266 MTPKDYK 272 +TP+ +K Sbjct: 234 VTPRQFK 240 >ref|NP_665006.1| putative two-component response regulator [Streptococcus pyogenes MGAS315] gb|AAM79809.1| putative two-component response regulator [Streptococcus pyogenes MGAS315] Length = 246 Score = 125 bits (313), Expect = 1e-27, Method: Compositional matrix adjust. Identities = 79/246 (32%), Positives = 138/246 (56%), Gaps = 8/246 (3%) Query: 27 VMIADDEEVIRRGLEKITSRMDLEVQVIGSYGNGLEAWNHLRELSQDDIDLLITDIKMPR 86 ++I +DE ++R+G+ + ++ + NG AW+ + ++ D+++TDI MP+ Sbjct: 4 LLIVEDEYLVRQGIRSLVDFSQFKIDRVNEAENGQLAWDLFQ---KEPYDIVLTDINMPK 60 Query: 87 MDGFKLIEEVRGHLKQLPIAVLSGFSEFEYARRAMRHGVLDYLLKPIEKAQLYDLLKSVE 146 ++G +L E ++ Q + L+G+ +F YA A++ G DYLLKP KA + D+L ++ Sbjct: 61 LNGIQLAELIKQESPQTHLVFLTGYDDFNYALSALKLGADDYLLKPFSKADVEDMLGKLQ 120 Query: 147 ENKKLRPVAAEPEEAPHQAGEGGEHYVVEQTKSILEKEYGQNFELERLAETVGMNASYIS 206 + L ++ E + E + V +I E+ + L+ LA+ +G + +Y+S Sbjct: 121 QKLDL----SKKTETIQELVEQPQKEVSAIAMAIHERLADSDLTLKSLAQQLGFSPNYLS 176 Query: 207 RLFKYKTGQTITDYLIGIRIAKAKELLIGQPDLKNYEIAERVGYSDPVYFNKLFKKMCGM 266 L K G DYL+ R+ KAK LL+ +LK YEIAE+VG+ D YF++ FK++ G+ Sbjct: 177 VLIKKDLGMPFQDYLVQERLKKAKLLLLT-SNLKIYEIAEQVGFEDMNYFSQRFKQLVGV 235 Query: 267 TPKDYK 272 TP YK Sbjct: 236 TPSQYK 241 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.319 0.138 0.393 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 419,129,415 Number of extensions: 17508071 Number of successful extensions: 65653 Number of sequences better than 1.0e-05: 1875 Number of HSP's gapped: 68499 Number of HSP's successfully gapped: 1903 Length of query: 276 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 152 Effective length of database: 420,565,302 Effective search space: 63925925904 Effective search space used: 63925925904 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 125 (52.8 bits)