BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0021.p (519 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00098267.1| COG0642: Signal transduction histidine kinase... 387 e-106 ref|NP_349365.1| Membrane-associated sensory histidine kinase wi... 242 2e-62 ref|ZP_00019355.1| hypothetical protein [Chloroflexus aurantiacus] 202 2e-50 ref|NP_739103.1| putative sensor kinase [Corynebacterium efficie... 191 3e-47 gb|AAR04622.1| PhoR [Rhodococcus equi] 183 9e-45 >ref|ZP_00098267.1| COG0642: Signal transduction histidine kinase [Desulfitobacterium hafniense] Length = 503 Score = 387 bits (994), Expect = e-106, Method: Compositional matrix adjust. Identities = 234/513 (45%), Positives = 302/513 (58%), Gaps = 32/513 (6%) Query: 14 APHSLRKQXXXXXXXXXXXXXXXXXXXQYVLMRDFIYSNRAESMETQIRSVPREIFSSPP 73 P+SLR Q QYV ++DFI + A S+ +QI SVPR+ ++ Sbjct: 12 TPNSLRFQLLSRSLFFLAIILLLIGIFQYVFLKDFILGSTAASIRSQIASVPRDSWTQML 71 Query: 74 AWGAGGIDAS-----GMSSGEAGSPDTGSPGNRPPGNLRNTGRRPLLLDAHTTIAVYSAD 128 + I+A + G G P NR P NL ++ TIA + Sbjct: 72 LYAE--INAKFNLDKDIVELVPGKTQEGPP-NRIPINLMDS-----------TIAFVDLN 117 Query: 129 GGFKDFQEETLSDSPAPRLTDAEYNDLLKHTTDKANGG-YRLMAAPDGSEHLAVFMNLGK 187 G T++ S AP L A D + D + + ++ + G+E L V Sbjct: 118 GNVTSLAN-TMNRSGAPPLLSA---DQYQQALDPSESPRFWVVNSESGTELLVVL----H 169 Query: 188 PQEARMLL----QMSVSTGPLKDVIMQQXXXXXXXXXXXXXXXXXXXXXXXRKTLVPLSN 243 P E R L Q+S+ST ++DV+ +Q +KTLVPLSN Sbjct: 170 PLELRGYLVGFIQVSLSTQFVQDVLFRQLSIFMLLSVLALVGGLVAYQAVIKKTLVPLSN 229 Query: 244 MGEAAEIIDAGNLSERFPVHQGQTEIDKLSRSFNGMLERLEISFHNEREAKEQMRRFAAD 303 + E +DAGNL+ER PV QGQ EID L+ S NGMLERLE SF E+EAKEQMRRF AD Sbjct: 230 IVHTMENMDAGNLNERLPVEQGQKEIDTLAVSLNGMLERLESSFTAEKEAKEQMRRFVAD 289 Query: 304 ASHELRTPLTSIHGFLEVLLRGAADNREQLMGALTSMHGESKRINKLVEDLLLLARMDGA 363 ASHELRTPLTSIHGFLEVLLRGA + ++L +LTSM+ ES+R+ KL++DLLLLA++D + Sbjct: 290 ASHELRTPLTSIHGFLEVLLRGAMNQPDKLQKSLTSMYAESERMKKLIQDLLLLAKLDRS 349 Query: 364 PQLNIKGMMLGEIIAEMQPQLLMLAGDRRVTFDISPGIRGMYDPDKLKQVILNLFQNAVQ 423 P L +G +I EM+PQL MLA +R + D++P D DK+KQVILNLF NAVQ Sbjct: 350 PYLEKSEKDIGVLILEMEPQLRMLAEEREIQLDLAPEELVPIDVDKMKQVILNLFHNAVQ 409 Query: 424 HTDAHKGTIHISLTARGTEAELAVQDNGSGIAAEHIPHVFERFYRSDSSRTRKYGGSGLG 483 HTD KG I ISL + + EL VQDNG GI EH+PH+FERFYRSDSSRTRKYGG+GLG Sbjct: 410 HTDPDKGLIQISLKSEASGVELTVQDNGPGIPEEHLPHLFERFYRSDSSRTRKYGGAGLG 469 Query: 484 LSITKSIVDAHHGGIRVESRPGEGTTFRVTLPR 516 L+IT S+V+ H G +RVES+ GEG+ F V LP+ Sbjct: 470 LAITHSLVELHGGTLRVESKVGEGSRFIVGLPK 502 >ref|NP_349365.1| Membrane-associated sensory histidine kinase with HAMP domain [Clostridium acetobutylicum] pir||F97239 membrane-associated sensory histidine kinase with HAMP domain [imported] - Clostridium acetobutylicum gb|AAK80705.1| Membrane-associated sensory histidine kinase with HAMP domain [Clostridium acetobutylicum] Length = 482 Score = 242 bits (618), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 138/414 (33%), Positives = 229/414 (55%), Gaps = 8/414 (1%) Query: 107 RNTG---RRPLLLDAHTTIAVYSADGGFKDFQEETLSDSPAPRLTDAEYNDLLKHTTDKA 163 +N+G ++ L D TT +V +G F + + P ++ +EY ++K + Sbjct: 72 KNSGYLIKKSLYFD--TTSSVIDKNGNVLLFHIKDKHPTSPPIISASEYKAIMKREGNLE 129 Query: 164 NGGYRLMAAPDGSEHLAVFMNLGKPQEARMLLQMSVSTGPLKDVIMQQXXXXXXXXXXXX 223 GY + G + ++ +G L+Q++ + +Q Sbjct: 130 --GYTITKNKCGEPQITIWRKIGNLNSPLGLIQLTTPYHSAAQFLYKQIYIYIVACVLIL 187 Query: 224 XXXXXXXXXXXRKTLVPLSNMGEAAEIIDAGNLSERFPVHQGQTEIDKLSRSFNGMLERL 283 + TL PL + + G+L++R + Q EID L+ +FN M R+ Sbjct: 188 ILSILIGGTLIKHTLKPLYRIAHTLNNLTVGDLNKRLSENNNQVEIDHLATAFNNMFSRI 247 Query: 284 EISFHNEREAKEQMRRFAADASHELRTPLTSIHGFLEVLLRGAADNREQLMGALTSMHGE 343 EISF+ E+E K++M++F +DASHELRTPLTSI GF+EVLL G+ + ++L AL ++ E Sbjct: 248 EISFNREKELKKKMQQFISDASHELRTPLTSIRGFVEVLLIGSYKDEKKLKLALNTILIE 307 Query: 344 SKRINKLVEDLLLLARMDGAPQLNIKGMMLGEIIAEMQPQLLMLAGDRRVTFDI-SPGIR 402 S+R+ +LV +LL L R+D + + + +II E+ PQL +LA +R+V ++ Sbjct: 308 SERLTELVNNLLTLTRLDRSTHTEMTKQNMSDIIEEIYPQLKILAKNRQVNLNLYDNNAY 367 Query: 403 GMYDPDKLKQVILNLFQNAVQHTDAHKGTIHISLTARGTEAELAVQDNGSGIAAEHIPHV 462 + + +++KQVI N+ QN++ +TD G I +SL+ T L + DNG GIA++ +PH+ Sbjct: 368 FLGNKNQIKQVIFNIVQNSINYTDKETGIITLSLSIEDTLFILKISDNGIGIASKDLPHI 427 Query: 463 FERFYRSDSSRTRKYGGSGLGLSITKSIVDAHHGGIRVESRPGEGTTFRVTLPR 516 F+RF+RS++ R+R+ GG GLGLSI KSI+ +H G I +S +GT+F + L R Sbjct: 428 FDRFFRSETHRSRESGGYGLGLSIAKSIIQSHGGEISTKSIINQGTSFYIKLKR 481 >ref|ZP_00019355.1| hypothetical protein [Chloroflexus aurantiacus] Length = 504 Score = 202 bits (513), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 124/322 (38%), Positives = 178/322 (55%), Gaps = 8/322 (2%) Query: 194 LLQMSVSTGPLKDVIMQQXXXXXXXXXXXXXXXXXXXXXXXRKTLVPLSNMGEAAEIIDA 253 ++QMS P++ + +Q TL PLS++ A + I Sbjct: 180 VVQMSTPLSPIETTLQRQSIIIVSSIVIVLLAGSLIGYWLTSSTLRPLSDLIVACQKIAQ 239 Query: 254 GNLSERFPVHQGQTEIDKLSRSFNGMLERLEISFHNEREAKEQMRRFAADASHELRTPLT 313 GNL +R PV EI KL+++F+ M+ERLE +F +R RF ADA+HE+RTPLT Sbjct: 240 GNLRQRVPVISPHDEIGKLTQAFDEMVERLEQNFQAQR-------RFIADAAHEMRTPLT 292 Query: 314 SIHGFLEVLLRGAADNREQLMGALTSMHGESKRINKLVEDLLLLARMDGAPQLNIKGMML 373 ++ LEVLLRGA D+ + + SMH + R+ + E LL AR + LNI+ + + Sbjct: 293 ALRSGLEVLLRGAQDDPQTAFRLIQSMHRDVVRLCNVSEQLLDKARYESGRSLNIQPVTM 352 Query: 374 GEIIAEMQPQLLMLAGDRRVTFDISPGIRGMYDPDKLKQVILNLFQNAVQHTDAHKGTIH 433 + + E Q +LAG+RRV + P I+ D D +KQ + +L NA+QHT A+ GTI Sbjct: 353 TDFLVEFTAQARLLAGERRVILESGPPIQAWIDGDSVKQALFHLVDNAIQHTAAN-GTIR 411 Query: 434 ISLTARGTEAELAVQDNGSGIAAEHIPHVFERFYRSDSSRTRKYGGSGLGLSITKSIVDA 493 + V DNG GIA +PHVF FYR D SR+R+ GG+GLGL++ +S+ A Sbjct: 412 LGWKVDQQRLCCWVSDNGEGIAEADLPHVFTPFYRGDRSRSRRTGGTGLGLTLVQSVAHA 471 Query: 494 HHGGIRVESRPGEGTTFRVTLP 515 H G + + SR GEGTT + LP Sbjct: 472 HGGEVAISSRQGEGTTVTIILP 493 >ref|NP_739103.1| putative sensor kinase [Corynebacterium efficiens YS-314] dbj|BAC19303.1| putative sensor kinase [Corynebacterium efficiens YS-314] Length = 475 Score = 191 bits (486), Expect = 3e-47, Method: Compositional matrix adjust. Identities = 113/288 (39%), Positives = 171/288 (59%), Gaps = 11/288 (3%) Query: 236 KTLVPLSNMGEAAEIIDAGNLSERFPVHQGQTEIDKLSRSFNGMLERLEISFHNEREAKE 295 ++L PL + + A+ I G+L R P TE+ +L+ + N MLE+L+ S + +E + Sbjct: 194 RSLRPLREVEKTAKSIAGGDLDRRVPQWPMTTEVGQLANALNIMLEQLQASILSAQEKES 253 Query: 296 QMRRFAADASHELRTPLTSIHGFLEVLLRGAADNREQLMGALTSMHGESKRINKLVEDLL 355 QMRRF DASHELRTPLTS+ G+ E+ GA + + + L+ + GE++R++ LVEDLL Sbjct: 254 QMRRFVGDASHELRTPLTSVKGYSELYHSGATRDADWV---LSKISGEAQRMSVLVEDLL 310 Query: 356 LLARMDGAPQLNIKGMMLGEIIAEMQPQLLMLAGDRRVTFDIS----PGIRGMYDPDKLK 411 L R +G Q+ + + + E+ + + +R +T P + G D +L Sbjct: 311 SLTRAEGQ-QMEKRPVDVLELSLSVASSMRAAWPERSITVVNKTGSLPVVEG--DATRLH 367 Query: 412 QVILNLFQNAVQHTDAHKGTIHISLTARGTEAELAVQDNGSGIAAEHIPHVFERFYRSDS 471 QV+ NL N + H ++ I ++A G + V D+G G+ AE H+FERFYR+D+ Sbjct: 368 QVLTNLVNNGLNH-GGPDASVEIEISAEGGSVLVRVVDDGVGMTAEDAQHIFERFYRTDT 426 Query: 472 SRTRKYGGSGLGLSITKSIVDAHHGGIRVESRPGEGTTFRVTLPRSVE 519 SR+R GGSGLGL+ITKS+V+ H G I V+S GEGT F +TLP +E Sbjct: 427 SRSRASGGSGLGLAITKSLVEGHRGTITVDSEVGEGTVFTITLPSRME 474 >gb|AAR04622.1| PhoR [Rhodococcus equi] Length = 464 Score = 183 bits (465), Expect = 9e-45, Method: Compositional matrix adjust. Identities = 122/292 (41%), Positives = 171/292 (58%), Gaps = 14/292 (4%) Query: 235 RKTLVPLSNMGEAAEIIDAGNLSERFPVHQGQTEIDKLSRSFNGMLERLEISFHNE---- 290 R++L PL + E A I AG+L R P +TE+ +LS + NGML +++ +F Sbjct: 176 RRSLRPLREVEEIAADIAAGDLHRRVPEWDQRTEVGRLSSALNGMLAQIQTAFAATAASE 235 Query: 291 ---REAKEQMRRFAADASHELRTPLTSIHGFLEVLLRGAADNREQLMGALTSMHGESKRI 347 R ++E+MRRF ADASHELRTPL +I GF E+ +GAA + E +M + + +++R+ Sbjct: 236 ESARRSEERMRRFVADASHELRTPLATIRGFAELYRQGAAPDPEMVM---SRIEDQAQRM 292 Query: 348 NKLVEDLLLLARMDGAPQLNIKGMMLGEIIAEMQPQLLMLAGDRRVTFDISPG---IRGM 404 LVEDLLLLARMD + L + A+ + RR+ ++ PG + Sbjct: 293 GLLVEDLLLLARMDAQRPFERHPVDLLAVAADAVHDARARSPQRRIMLEVLPGSAPAEVL 352 Query: 405 YDPDKLKQVILNLFQNAVQHTDAHKGTIHISLTARGTEAELAVQDNGSGIAAEHIPHVFE 464 D +L+QV+ NL NA+ HT A + + + G +A + V D G G+A VFE Sbjct: 353 GDDARLRQVVGNLVGNALDHTPA-GADVTVRVGTDGADAVVEVADTGPGLAEGDAARVFE 411 Query: 465 RFYRSDSSRTRKYGGSGLGLSITKSIVDAHHGGIRVESRPGEGTTFRVTLPR 516 RFYR+D+SRTR GGSGLGLSI +IV AH G + V+S PG G TFRV LPR Sbjct: 412 RFYRADASRTRTSGGSGLGLSIVAAIVAAHGGTVDVDSAPGNGATFRVRLPR 463 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.317 0.134 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 689,790,527 Number of extensions: 28222039 Number of successful extensions: 96649 Number of sequences better than 1.0e-05: 5525 Number of HSP's gapped: 88792 Number of HSP's successfully gapped: 5553 Length of query: 519 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 389 Effective length of database: 404,274,708 Effective search space: 157262861412 Effective search space used: 157262861412 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 128 (53.9 bits)