BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0303.0002.p (132 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_388343.1| similar to holo- acyl-carrier protein synthase ... 91 9e-18 pdb|1F7L|A Chain A, Holo-(Acyl Carrier Protein) Synthase In Comp... 90 2e-17 pdb|1F7T|A Chain A, Holo-(Acyl Carrier Protein) Synthase At 1.8a... 89 2e-17 pdb|1F80|A Chain A, Holo-(Acyl Carrier Protein) Synthase In Comp... 88 5e-17 ref|ZP_00043556.1| COG0736: Phosphopantetheinyl transferase (hol... 87 1e-16 >ref|NP_388343.1| similar to holo- acyl-carrier protein synthase [Bacillus subtilis] sp|P96618|ACPS_BACSU Holo-[acyl-carrier protein] synthase (Holo-ACP synthase) (4'-phosphopantetheinyl transferase acpS) pir||H69772 holo-[acyl-carrier-protein] synthase (EC 2.7.8.7) - Bacillus subtilis dbj|BAA19299.1| FUNCTION UNKNOWN, SIMILAR PRODUCT IN E. COLI AND MYCOPLASMA PNEUMONIAE. [Bacillus subtilis] emb|CAB12269.1| holo-acyl carrier protein synthase [Bacillus subtilis subsp. subtilis str. 168] Length = 121 Score = 90.9 bits (224), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 57/133 (42%), Positives = 72/133 (54%), Gaps = 18/133 (13%) Query: 1 VIYGIGHDVLEIGRVAGIANGSLGSRFTRRILTAQEL-VLXXXXXXXXXEFIAGRFSAKE 59 +IYGIG D+ E+ R+A +A RF RILT EL EF+AGRF+AKE Sbjct: 1 MIYGIGLDITELKRIASMAGRQ--KRFAERILTRSELDQYYELSEKRKNEFLAGRFAAKE 58 Query: 60 AVVKALGCGIGHTVGFQDIEILPDALGKP---VASLSAEAWSRLRLPEQEYTIHLTITHS 116 A KA G GIG + FQDIEI D GKP LS A +H++ITH+ Sbjct: 59 AFSKAFGTGIGRQLSFQDIEIRKDQNGKPYIICTKLSQAA------------VHVSITHT 106 Query: 117 RGLASAFAVVERL 129 + A+A V+ERL Sbjct: 107 KEYAAAQVVIERL 119 >pdb|1F7L|A Chain A, Holo-(Acyl Carrier Protein) Synthase In Complex With Coenzyme A At 1.5a Length = 121 Score = 89.7 bits (221), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 57/132 (43%), Positives = 71/132 (53%), Gaps = 18/132 (13%) Query: 2 IYGIGHDVLEIGRVAGIANGSLGSRFTRRILTAQEL-VLXXXXXXXXXEFIAGRFSAKEA 60 IYGIG D+ E+ R+A +A RF RILT EL EF+AGRF+AKEA Sbjct: 2 IYGIGLDITELKRIASMAGRQ--KRFAERILTRSELDQYYELSEKRKNEFLAGRFAAKEA 59 Query: 61 VVKALGCGIGHTVGFQDIEILPDALGKP---VASLSAEAWSRLRLPEQEYTIHLTITHSR 117 KA G GIG + FQDIEI D GKP LS A +H++ITH++ Sbjct: 60 FSKAFGTGIGRQLSFQDIEIRKDQNGKPYIICTKLSPAA------------VHVSITHTK 107 Query: 118 GLASAFAVVERL 129 A+A V+ERL Sbjct: 108 EYAAAQVVIERL 119 >pdb|1F7T|A Chain A, Holo-(Acyl Carrier Protein) Synthase At 1.8a pdb|1F7T|B Chain B, Holo-(Acyl Carrier Protein) Synthase At 1.8a pdb|1F7T|C Chain C, Holo-(Acyl Carrier Protein) Synthase At 1.8a pdb|1F7T|D Chain D, Holo-(Acyl Carrier Protein) Synthase At 1.8a pdb|1F7T|E Chain E, Holo-(Acyl Carrier Protein) Synthase At 1.8a pdb|1F7T|F Chain F, Holo-(Acyl Carrier Protein) Synthase At 1.8a Length = 122 Score = 89.4 bits (220), Expect = 2e-17, Method: Compositional matrix adjust. Identities = 57/132 (43%), Positives = 71/132 (53%), Gaps = 18/132 (13%) Query: 2 IYGIGHDVLEIGRVAGIANGSLGSRFTRRILTAQEL-VLXXXXXXXXXEFIAGRFSAKEA 60 IYGIG D+ E+ R+A +A RF RILT EL EF+AGRF+AKEA Sbjct: 3 IYGIGLDITELKRIASMAGRQ--KRFAERILTRSELDQYYELSEKRKNEFLAGRFAAKEA 60 Query: 61 VVKALGCGIGHTVGFQDIEILPDALGKP---VASLSAEAWSRLRLPEQEYTIHLTITHSR 117 KA G GIG + FQDIEI D GKP LS A +H++ITH++ Sbjct: 61 FSKAFGTGIGRQLSFQDIEIRKDQNGKPYIICTKLSPAA------------VHVSITHTK 108 Query: 118 GLASAFAVVERL 129 A+A V+ERL Sbjct: 109 EYAAAQVVIERL 120 >pdb|1F80|A Chain A, Holo-(Acyl Carrier Protein) Synthase In Complex With Holo- (Acyl Carrier Protein) pdb|1F80|B Chain B, Holo-(Acyl Carrier Protein) Synthase In Complex With Holo- (Acyl Carrier Protein) pdb|1F80|C Chain C, Holo-(Acyl Carrier Protein) Synthase In Complex With Holo- (Acyl Carrier Protein) Length = 120 Score = 88.2 bits (217), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 56/131 (42%), Positives = 70/131 (53%), Gaps = 18/131 (13%) Query: 3 YGIGHDVLEIGRVAGIANGSLGSRFTRRILTAQEL-VLXXXXXXXXXEFIAGRFSAKEAV 61 YGIG D+ E+ R+A +A RF RILT EL EF+AGRF+AKEA Sbjct: 2 YGIGLDITELKRIASMAGRQ--KRFAERILTRSELDQYYELSEARKNEFLAGRFAAKEAF 59 Query: 62 VKALGCGIGHTVGFQDIEILPDALGKP---VASLSAEAWSRLRLPEQEYTIHLTITHSRG 118 KA G GIG + FQDIEI D GKP LS A +H++ITH++ Sbjct: 60 SKAFGTGIGRQLSFQDIEIRKDQNGKPYIICTKLSQAA------------VHVSITHTKE 107 Query: 119 LASAFAVVERL 129 A+A V+ERL Sbjct: 108 YAAAQVVIERL 118 >ref|ZP_00043556.1| COG0736: Phosphopantetheinyl transferase (holo-ACP synthase) [Magnetococcus sp. MC-1] Length = 138 Score = 87.0 bits (214), Expect = 1e-16, Method: Compositional matrix adjust. Identities = 52/130 (40%), Positives = 72/130 (55%), Gaps = 4/130 (3%) Query: 1 VIYGIGHDVLEIGRVAGIANGSLGSRFTRRILTAQELVLXXXXXXXXXEFI-AGRFSAKE 59 +I GIG+D++EI R+ + N G RF R T QE L A RF+AKE Sbjct: 1 MIVGIGNDLVEIQRLTNMLNKGHGQRFLERCFTQQEQQLCNGRSTPQRACCYAKRFAAKE 60 Query: 60 AVVKALGCGIGHTVGFQDIEILPDALGKPVASL--SAEAWSRLRLPEQEYTIHLTITHSR 117 AV+KALG G + F DIE+LP+ LG+P +L + W P Q IHL+++ + Sbjct: 61 AVIKALGVGFRQGLWFTDIEVLPNQLGRPTVTLHGATAQWLACHHPGQ-VNIHLSLSDEK 119 Query: 118 GLASAFAVVE 127 GLA A A++E Sbjct: 120 GLAIATALIE 129 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.323 0.140 0.404 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 164,238,213 Number of extensions: 5081426 Number of successful extensions: 12300 Number of sequences better than 1.0e-05: 232 Number of HSP's gapped: 12271 Number of HSP's successfully gapped: 232 Length of query: 132 Length of database: 757,237,578 Length adjustment: 95 Effective length of query: 37 Effective length of database: 499,303,173 Effective search space: 18474217401 Effective search space used: 18474217401 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 120 (50.8 bits)