BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0017.p (444 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value pir||S63614 malE protein homolog cymE precursor - Klebsiella oxy... 241 2e-62 dbj|BAB40635.1| periplasmic binding protein [Thermoactinomyces v... 238 2e-61 ref|NP_693481.1| maltose:maltodextrin transport system maltose:m... 228 2e-58 ref|NP_980358.1| maltosaccharide ABC transporter, maltosaccharid... 209 8e-53 ref|NP_658050.1| SBP_bacterial_1, Bacterial extracellular solute... 209 2e-52 >pir||S63614 malE protein homolog cymE precursor - Klebsiella oxytoca emb|CAA60004.1| cymE [Klebsiella oxytoca] Length = 409 Score = 241 bits (616), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 134/379 (35%), Positives = 211/379 (55%), Gaps = 4/379 (1%) Query: 61 IKPEEGASLLVWESKEERVFAEEIAKQFTAKYNVPVKI--EEVAPPDQVGKLTQDGPSGL 118 I PE+ A+LL+W + + A+ F YN V+ +AP D ++ QDG S Sbjct: 26 IAPEKQANLLIWTDISTLDYMKYAAENFNKTYNSQVRFTFRGLAPIDAASRMIQDGGSTR 85 Query: 119 AADVVLIPHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYA 178 ADV I HD LG+ A ++ N I AE+ +I S Y+G YG+P + T A Sbjct: 86 VADVAEIEHDLLGRLVVAGGVMENLISAERINKKFMSNAINASRYEGNNYGFPVSYATVA 145 Query: 179 LYYNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFGKD 238 L+YNK +++ P F+++I F + +NKY ++W+V N Y F+ G Y FG++ Sbjct: 146 LFYNKDILRLPPDKFEELIQLSAEFNNVKENKYTLLWDVQNYYESRMFLTLYGAYEFGQE 205 Query: 239 GTDKDDIGLNNEGAIEGLKTFVKLKEVLPIKSGDI-NSDIKRSLFSSGDVAMDMTGPWEL 297 GT+ D+G+N+E A +GL +LK P D+ N ++R LFS G VA + GPW + Sbjct: 206 GTNVKDLGINSELAQKGLAAMKRLKTANPSNPVDMRNPQVRRGLFSEGKVAAIIDGPWAI 265 Query: 298 AAYKQALGDKLGVAPVPTVDGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLE 357 Y + GV P+PT++GK +FS +++ V++Y++ P AA+L+A++ SS E ++ Sbjct: 266 QGYNNS-KINFGVVPIPTLEGKQPRSFSTVRLAVVSSYSKSPKAAQLFADYLSSDEMLMK 324 Query: 358 LNKLVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIW 417 ++ SIP + T + SA +Q +S MPSIPEMG +WSP+ A+ ++W Sbjct: 325 RYEMTESIPPVPNLMNKILPTANEATSAIIKQGLHSDAMPSIPEMGYLWSPLANAITDMW 384 Query: 418 DNNADPKAAMDKAVQQIKD 436 N+ PKAA+D+A I + Sbjct: 385 INDQTPKAALDRARNIIDE 403 >dbj|BAB40635.1| periplasmic binding protein [Thermoactinomyces vulgaris] Length = 417 Score = 238 bits (607), Expect = 2e-61, Method: Compositional matrix adjust. Identities = 143/375 (38%), Positives = 213/375 (56%), Gaps = 12/375 (3%) Query: 69 LLVWESKEERVF---AEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLAADVVLI 125 L+VWE+ ++ V ++ A +FT K + V++ VA Q KLT DGP+G AD+V Sbjct: 41 LVVWENADDGVQLNNTKKWAGEFTKKTGIQVEVVPVALLKQQEKLTLDGPAGKGADLVTW 100 Query: 126 PHDNLGKAASASLLLPNDIFAEQTKAANTEASIVGSSYDGELYGYPRAAETYALYYNKSL 185 PHD LG+A + LL P + K + ++ +Y G+LYG P+A E+ AL YNK L Sbjct: 101 PHDRLGEAVTKGLLQPIQV-DNSVKNQFDDVAMKALTYGGKLYGLPKAIESVALIYNKKL 159 Query: 186 VKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFG-KDGT-DKD 243 + + P T+D++ + K + KYG+++E N Y+ Y A G +F +DGT D + Sbjct: 160 MGQVPATYDELFQYAKANNKPDEQKYGVLFEANNFYYTYFLFAAKGAAVFKEQDGTLDPN 219 Query: 244 DIGLNNEGAIEGLKTFVK--LKEVLPIKSGDINSDIKRSLFSSGDVAMDMTGPWELAAYK 301 +IGLN+ A++G+ K + LP + +D LF SG VA + GPW + Y Sbjct: 220 EIGLNSPEAVQGMNEVQKWFTEARLP---QSLKADTVNGLFKSGKVAAVINGPWAIKDY- 275 Query: 302 QALGDKLGVAPVPTVDGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLELNKL 361 QA G +GVAP+P +DGK A TF G+K + ++AY++YP A +F +SKE+ K Sbjct: 276 QAAGINVGVAPLPKIDGKDAQTFIGVKGWYLSAYSKYPKYATELMQFLTSKEALASRFKE 335 Query: 362 VGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIWDNNA 421 G IP E L DP I +P ++ FA+QA PMPSIPEMG VW P+N A + Sbjct: 336 TGEIPPQKELLNDPMIKNNPVVNGFAKQASKGVPMPSIPEMGVVWEPINNAHTFVAQGKQ 395 Query: 422 DPKAAMDKAVQQIKD 436 P+ A++ AV+ +K+ Sbjct: 396 TPEQALNDAVKIMKE 410 >ref|NP_693481.1| maltose:maltodextrin transport system maltose:maltodextrin-binding protein [Oceanobacillus iheyensis HTE831] dbj|BAC14516.1| maltose:maltodextrin transport system maltose:maltodextrin-binding protein [Oceanobacillus iheyensis HTE831] Length = 425 Score = 228 bits (581), Expect = 2e-58, Method: Compositional matrix adjust. Identities = 133/383 (34%), Positives = 221/383 (57%), Gaps = 19/383 (4%) Query: 62 KPEEGASLLVW-ESKEERVFA-EEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLA 119 KP+E L++W +EE++ A E I ++F + + VK+E + DQ+ +L+ GP G Sbjct: 43 KPDE---LVIWVNDQEEQIEAIENITQKFEEQEGIQVKLETKSMLDQMQELSLAGPEGNG 99 Query: 120 ADVVLIPHDNLGKAASASLLLPNDIFAEQ-TKAANTEASIVGSSYDGE--LYGYPRAAET 176 D+ PHD +G + L P D+ EQ + +T V +YDG +YG P ET Sbjct: 100 PDLFFQPHDRIGNIVAQGLAEPVDLTDEQLNEYVDTAVEAVTYTYDGNTNIYGIPAVIET 159 Query: 177 YALYYNKSLVKEAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFG 236 Y ++YNK+L +EAP+T +D++ + ++ +++++G + ++YF +PF G Y+FG Sbjct: 160 YGIFYNKALQEEAPETIEDLLTVLEENSNPSEDQFGFLMRPNDLYFNFPFFKNYGAYIFG 219 Query: 237 KD--GTDKDDIGLNNEGAIEGLKTFVKL--KEVLPIKSGDINSDIKRSLFSSGDVAMDMT 292 D D +DIGL N+GAIEG + + + +P + D+ LF+ G V ++ Sbjct: 220 GDTGNYDIEDIGLANDGAIEGAELYQSFFGEGKIPTST---TGDVIDGLFTEGKVGSVIS 276 Query: 293 GPWELAAYKQALGDKLGVAPVPTVDGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSK 352 GPW + YK+ALG+ LG AP P ++ + TF G+K + V+ Y++ AK A F +++ Sbjct: 277 GPWSVPGYKEALGEDLGFAPFPMINDQPTETFVGVKSWMVSYYSENKEWAKELAVFMTNE 336 Query: 353 ESQLELNKLVGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAA 412 E+Q E ++ G +P N +AL + + DP +AFAEQ ++ PMPS PEM VW P+N A Sbjct: 337 ENQGEYFEIAGELPPNAKALSNIE---DPIYAAFAEQIEHGFPMPSAPEMAQVWEPMNNA 393 Query: 413 LPEIWDNNADPKAAMDKAVQQIK 435 L + DPK +++A++Q++ Sbjct: 394 L-QFLAEGEDPKTVLEEALEQVQ 415 >ref|NP_980358.1| maltosaccharide ABC transporter, maltosaccharide-binding protein, putative [Bacillus cereus ATCC 10987] gb|AAS42966.1| maltosaccharide ABC transporter, maltosaccharide-binding protein, putative [Bacillus cereus ATCC 10987] Length = 419 Score = 209 bits (533), Expect = 8e-53, Method: Compositional matrix adjust. Identities = 145/374 (38%), Positives = 210/374 (56%), Gaps = 12/374 (3%) Query: 69 LLVWESKEERVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLAADVVLIPHD 128 LLVWE ++ V E K F KY V VK+ E+ DQV KL DGP+G DVV +PHD Sbjct: 40 LLVWEDDKKGVGLEPAVKSFEEKYKVKVKVVEMQMTDQVKKLRLDGPAGTGPDVVTLPHD 99 Query: 129 NLGKAASASLLLPNDIFAEQT-KAANTEASIVGSSYDGELYGYPRAAETYALYYNKSLVK 187 +G A + LL +++ A+ + K+ T++SI +Y+G+LYG P+A ET YNK L+ Sbjct: 100 QIGNAVTEGLL--SEVKADDSIKSKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMP 157 Query: 188 EAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFG-KDGT-DKDDI 245 +AP+T D++ F K FT K YG + N YF F+A GGY+FG KDG + D+ Sbjct: 158 KAPETMDELFNFSKEFTKDGK--YGFLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDV 215 Query: 246 GLNNEGAIEGLKTFVK-LKEVLPIKS--GDINSDIKRSLFSSGDVAMDMTGPWELAAYKQ 302 GLNN GA++G + K KE L K G+ LF+ G A M GPW A ++ Sbjct: 216 GLNNSGAVQGAEYLQKWYKEKLFPKGIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEK 275 Query: 303 ALGDKLGVAPVPTV-DGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLELNKL 361 G GVAP+P + +G+ TF G+K + V ++++ + A + E+ +++E+ + Sbjct: 276 N-GIDYGVAPMPKLPNGQPMKTFVGVKGWHVTSFSKQNDLATKFTEWVTNEENAKIRFEK 334 Query: 362 VGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIWDNNA 421 IP ++DP I + A A Q++N PMP+IPEM VW P AL + + Sbjct: 335 TKEIPPVKAVMEDPIIKDNEAAKAVATQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKE 394 Query: 422 DPKAAMDKAVQQIK 435 PKAA+D AV+QIK Sbjct: 395 APKAALDNAVKQIK 408 >ref|NP_658050.1| SBP_bacterial_1, Bacterial extracellular solute-binding protein [Bacillus anthracis A2012] ref|NP_846464.1| maltosaccharide ABC transporter, maltosaccharide-binding protein, putative [Bacillus anthracis str. Ames] gb|AAP27950.1| maltosaccharide ABC transporter, maltosaccharide-binding protein, putative [Bacillus anthracis str. Ames] Length = 419 Score = 209 bits (531), Expect = 2e-52, Method: Compositional matrix adjust. Identities = 145/374 (38%), Positives = 209/374 (55%), Gaps = 12/374 (3%) Query: 69 LLVWESKEERVFAEEIAKQFTAKYNVPVKIEEVAPPDQVGKLTQDGPSGLAADVVLIPHD 128 LLVWE ++ V E K F KY V VK+ E+ DQV KL DGP+G DVV +PHD Sbjct: 40 LLVWEDDKKGVGLEPAVKSFEEKYKVKVKVVEMQMTDQVKKLRLDGPAGTGPDVVTLPHD 99 Query: 129 NLGKAASASLLLPNDIFAEQ-TKAANTEASIVGSSYDGELYGYPRAAETYALYYNKSLVK 187 +G A + LL +++ A+ K+ T++SI +Y+G+LYG P+A ET YNK L+ Sbjct: 100 QIGNAVTEGLL--SEVKADDAVKSKFTDSSIEAQTYNGKLYGLPKAIETPVFIYNKKLMP 157 Query: 188 EAPKTFDDVIAFGKTFTDKAKNKYGIMWEVGNMYFGYPFIATTGGYLFG-KDGT-DKDDI 245 +AP+T D++ F K FT K YG + N YF F+A GGY+FG KDG + D+ Sbjct: 158 KAPETMDELFNFSKEFTKDGK--YGFLALGDNFYFANAFMAGMGGYVFGEKDGKPNASDV 215 Query: 246 GLNNEGAIEGLKTFVK-LKEVLPIKS--GDINSDIKRSLFSSGDVAMDMTGPWELAAYKQ 302 GLNN GA++G + K KE L K G+ LF+ G A M GPW A ++ Sbjct: 216 GLNNSGAVQGAEYLQKWYKEKLFPKGIIGESGGPAADGLFNEGKAASIMNGPWAFQAMEK 275 Query: 303 ALGDKLGVAPVPTV-DGKTAITFSGIKIFAVNAYTQYPNAAKLYAEFASSKESQLELNKL 361 G GVAP+P + +G+ TF G+K + V ++++ + A + E+ +++E+ + Sbjct: 276 N-GIDYGVAPMPKLPNGQPLKTFVGVKGWHVTSFSKQNDLATKFTEWVTNEENAKIRFEK 334 Query: 362 VGSIPTNNEALKDPQITGDPYISAFAEQAKNSQPMPSIPEMGNVWSPVNAALPEIWDNNA 421 IP ++DP I + A A Q++N PMP+IPEM VW P AL + + Sbjct: 335 TKEIPPVKAVMEDPIIKDNEAAKAVATQSENGIPMPNIPEMQEVWKPAGDALQLVVTDKE 394 Query: 422 DPKAAMDKAVQQIK 435 PKAA+D AV+QIK Sbjct: 395 APKAALDSAVKQIK 408 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.312 0.131 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 623,929,069 Number of extensions: 27212132 Number of successful extensions: 51056 Number of sequences better than 1.0e-05: 208 Number of HSP's gapped: 50628 Number of HSP's successfully gapped: 208 Length of query: 444 Length of database: 757,237,578 Length adjustment: 129 Effective length of query: 315 Effective length of database: 406,989,807 Effective search space: 128201789205 Effective search space used: 128201789205 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 127 (53.5 bits)