BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0301.0014.p (2165 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value dbj|BAA11332.1| alkaline amylopullulanase [Bacillus sp.] 850 0.0 ref|NP_244564.1| alkaline amylopullulanase [Bacillus halodurans]... 827 0.0 dbj|BAB47586.1| alkaline pullulanase [Bacillus sp. KSM-1876] 808 0.0 emb|CAD32942.1| pullulanase [Streptococcus pyogenes] 672 0.0 ref|NP_269940.1| putative pullulanase [Streptococcus pyogenes] >... 671 0.0 >dbj|BAA11332.1| alkaline amylopullulanase [Bacillus sp.] Length = 1938 Score = 850 bits (2195), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/843 (51%), Positives = 570/843 (67%), Gaps = 27/843 (3%) Query: 2 DVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVSL 61 D+ LK DA KIGFL VN+ SG + G D TF +L +YNQL++K+G+D VY +P+G P++L Sbjct: 993 DIKLKEDANKIGFLFVNKQSGGQTG-DMTFDMLKQYNQLFVKEGEDKVYTNPYGTVPLAL 1051 Query: 62 VSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDTAAF 121 VS E+LS + I LTFT T+GLD LK + + D +G V I+ +V V F Sbjct: 1052 VSGEVLSDKLISLTFTRTEGLDLEELKEQLEIKDVDGNDVSFTDVTIEGEKTVHVH-GEF 1110 Query: 122 ALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASSVV 181 LEK P SV Y + +S +GW++IDEMY Y G LGA H D +ATLK+W+P A +V Sbjct: 1111 DLEKIPFSVTYLDRTISVKSGWKLIDEMYAYDGK-LGAELH-EDGTATLKVWSPKADNVS 1168 Query: 182 ANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGVTK 241 +Y K D + V + + GD+GVWS +L + + ++GYYY YE+T+ VT Sbjct: 1169 VVLYDKVDQNEVVDTIEMVKGDRGVWSVKLTKDN----TGLDSLKGYYYHYEITHGDVTN 1224 Query: 242 PVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDAVI 301 LDPYAKSMA + ++ GD VGKAAIVD+ G+ P+ DIPG+EKRED +I Sbjct: 1225 LALDPYAKSMAAWNNEA-------GDKVGKAAIVDI-GSIGPELDYADIPGFEKREDTII 1276 Query: 302 YEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDETRM 361 YEVHVRDFTSD +I +L + +G++A+F +KLDYI+ LG+THIQL+PVM++Y +E Sbjct: 1277 YEVHVRDFTSDPNIGEDLKAQ-FGTFASFVEKLDYIQELGVTHIQLLPVMSYYFSNEFES 1335 Query: 362 THRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGVIL 421 R Y++ YNWGYDPH+YFS G YS+ P DPE RIKE K LI+ +H+ MGV+L Sbjct: 1336 GERMLEYASTGTNYNWGYDPHNYFSLSGMYSENPEDPELRIKEFKNLINEIHKRDMGVVL 1395 Query: 422 DVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXX-XLATSHKMAEKLMVDSVKYWFSE 480 DVV+ H A+ +D+VPNYY F DA L T+H+M+ +++VDS+K+W E Sbjct: 1396 DVVFNHTAQVHIFEDLVPNYYHFMDADGTPRTSFGGGRLGTTHEMSRRVLVDSIKHWVDE 1455 Query: 481 YKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGADQA 540 YK+DG R+DMMG +NP + IGEGW TFAGD +P A ADQ Sbjct: 1456 YKVDGFRFDMMGDHDAESIQIAFDEAKKLNPNIVMIGEGWVTFAGDEGEPVQA---ADQQ 1512 Query: 541 WMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDPGD 600 WM T++VG FSDEFRNELKSG+GSEG+PRFITGGA ++ IF+NI A+P N AD PGD Sbjct: 1513 WMQYTEAVGSFSDEFRNELKSGFGSEGQPRFITGGAVNVQQIFDNIKAQPHNFMADQPGD 1572 Query: 601 VVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQEY 660 VV YIEAHDNLTL+DVIAQ+IKKDP I N+LEI +RIR+GN + +TSQGTAF+ AGQE+ Sbjct: 1573 VVQYIEAHDNLTLYDVIAQSIKKDPEIAENDLEIHKRIRVGNAMVLTSQGTAFLHAGQEF 1632 Query: 661 GRTKQWKA-AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVNYPV 717 GRTKQW+A A K T M DA+G F YFIHDSYDSSD +N FDW KATD YPV Sbjct: 1633 GRTKQWRAPATEAPYKSTYMTDADGNPFVYPYFIHDSYDSSDIINRFDWEKATDAEKYPV 1692 Query: 718 QNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDGTGL 777 N TR YT+GLI+LR+S+DAFRLG + LVD+NV ++ APEIK DLV+ Y++ +T G Sbjct: 1693 NNVTRDYTAGLIELRRSSDAFRLGSRELVDSNVTMVDAPEIKEQDLVVAYRSVSTAGVE- 1751 Query: 778 YYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPLTAV 837 YY F+N D ++RTLTL +DLT G V+VD ++A V + +GF LT+ ITL+PLT V Sbjct: 1752 YYTFVNADTSSRTLTLGQDLTEGVVVVDAEEANVAGVA--EPAGFELTAEGITLEPLTTV 1809 Query: 838 VIR 840 V+R Sbjct: 1810 VVR 1812 Score = 710 bits (1833), Expect = 0.0, Method: Compositional matrix adjust. Identities = 381/798 (47%), Positives = 522/798 (65%), Gaps = 34/798 (4%) Query: 938 RYVQITYVRDDKDYADWNLWVWNTGVKNDQIDFTTFKDGKASVLIEVAPNATSVGFVLRK 997 + ++ +Y R D +Y WNLWVW TGVK+DQIDFT FK+GKA I V+ NA VGF++RK Sbjct: 37 KRIEFSYERPDGNYEGWNLWVWGTGVKDDQIDFTEFKEGKAYADIAVSDNADKVGFIIRK 96 Query: 998 GTDWNTGKQDYPDDRVIPLTAGGGVXXXXVNVTSMVKELDIKPVINGPVMKDGTITFRYR 1057 G DW ++D+ DR I + + V+VTS ++ P + PV+ DG F +R Sbjct: 97 G-DWE--EKDFDGDRSITINKIDNITK--VHVTSQQEKFGQIPDGSPPVVADGNADFFFR 151 Query: 1058 DDALFRTDQQETISGVKVKVNGKEYPMAYEAAEEWFSYKLQDVQEGTYKYSFLVTKDGVT 1117 D L+ + + + V++ + G++Y M E +E F+Y L D+ G ++Y++LVT DG T Sbjct: 152 DKELYAAGEMDKVEKVELSILGEKYEMNGEPEKERFTYTLSDLPTGEHEYTYLVTVDGQT 211 Query: 1118 EELTDPKNTVNGESVVVYHIPKVAITAEVQPEAINSNENAVVTVKAASEEQVSYVDGYMD 1177 EE+TDP NTV+G SVV Y V ++A P ++ N+NAVV V SE + + ++ Sbjct: 212 EEVTDPYNTVDGRSVVEYVTSDVQVSASFIPAKVDYNQNAVVKVDIESETETKIREMSIN 271 Query: 1178 LSALGGPEAVKLDTELLQQTVAVKNSVPAGLKSIPITLLDQYGNTHKGTAQIEVKARTYT 1237 LS +GG E +D L + TVAVK V AG+K++PIT +D++GN H+G+A +EV+ART T Sbjct: 272 LSEIGGKEKATIDPALNELTVAVKQGVTAGVKNLPITAIDEFGNRHEGSATLEVQARTIT 331 Query: 1238 GGQLDFDWDEARIYFALTDRFKDGDATNN----ADVDTTHPEAYHGGDFRGMIDSLDYLQ 1293 G + DFDWD++ +YF LTDRF DGD++NN DT+ Y GGDF+G+ LDYL Sbjct: 332 GEKADFDWDQSVVYFMLTDRFFDGDSSNNDPHGIGYDTSKSGTYQGGDFKGITQRLDYLD 391 Query: 1294 KLGINTLWITPVVDNIDFN----KGADFGGTQYGYHGYWAKDFTQLDEHLGDMATFKELI 1349 +LGINT+WI+PVVDNI F+ +G D Y YHGYWA +F +L+ H G MA F E+I Sbjct: 392 ELGINTIWISPVVDNIKFDVRHSEGPD--TPYYAYHGYWADNFGELNPHFGSMADFHEMI 449 Query: 1350 EKAHDKGIKIMVDVVLNHAGYGLKAED-----NYPGVTAEDKARFDGMLRKDGVSADTDP 1404 + AH++GIKIMVDVVLNH GYGLK D N+P T ED+ARFDGMLR DG S + Sbjct: 450 DAAHERGIKIMVDVVLNHTGYGLKPGDSSSVANFP--TDEDRARFDGMLR-DGGSGE--- 503 Query: 1405 IKGELAGLPDFKTEDPAVRQTLIGWQTGWLDNARTERGDTIDYFRVDTVKHVDSTTWKAF 1464 ++GELAGLPDF TE+P VR+ ++ WQT W++ +RT +G+TIDYFRVDTVKHV+ TTW AF Sbjct: 504 VRGELAGLPDFLTENPDVREQVVQWQTDWIEKSRTAKGNTIDYFRVDTVKHVEDTTWMAF 563 Query: 1465 KNALTAIDPAFKLTGEYFGGTLDNDGGNLHSGQMDGLLDFGFKNAAKDFTDGKISAVDTY 1524 KNALT P KL GE +G +++D G L+SG MD LLDF FKN A+DF +G++ AV Sbjct: 564 KNALTKAMPEHKLIGEAWGANVNDDLGYLNSGMMDSLLDFDFKNYARDFANGQLDAVQQK 623 Query: 1525 LQHREAQMDNTGMMAQFLSSHDENGFLSNYVDGDKSKLKIAAALQITAKGQPVIYYGEEL 1584 L+ R ++++NT + QFL SHDE+ F V+GD K ++AA+LQ+TAKGQPVIYYGEEL Sbjct: 624 LEARNSKLNNTATLGQFLGSHDEDRFYE-VVEGDLGKYQVAASLQLTAKGQPVIYYGEEL 682 Query: 1585 GRSGANSGDLSKGEFSENRGDMPWDQLSAEQGLHDHYQKLLNIRAKYSQIYSKGIRTKLA 1644 G G N + NR +MPWD + + L +HYQKLL R ++KG R K+A Sbjct: 683 GLPGKND-----YPYYTNRQNMPWDDVDGNEIL-EHYQKLLAFRNDNPNTFAKGDRKKVA 736 Query: 1645 GSDELGYLAFDKQYDGANIVTAINTKAEAVSVSLPVPFAAQAAVVDEYSGKTYTVSGDQK 1704 GSD GYL F + Y ++ +NT+A A V+L +++A V D YSG+ Y + + + Sbjct: 737 GSDSEGYLLFSRTYGENSVYVGLNTEAAAKDVTLNFG-SSEAVVTDRYSGQEYQANEEGQ 795 Query: 1705 VRVELPGRGDGGTVILAV 1722 V +P DGGTV+L V Sbjct: 796 VTFSIPAMEDGGTVLLEV 813 Score = 62.8 bits (151), Expect = 9e-08, Method: Compositional matrix adjust. Identities = 33/62 (53%), Positives = 41/62 (66%), Gaps = 5/62 (8%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLD-RYNQLWIKQGDDHVYVSPFGEQPV 59 VDV L DAQ IGFLV+N ++GDKDGGDK L N++WIKQG D V++ +PV Sbjct: 884 VDVELAEDAQNIGFLVLNTTNGDKDGGDKAVELFSPDLNEIWIKQGSDEVFLY----EPV 939 Query: 60 SL 61 L Sbjct: 940 DL 941 >ref|NP_244564.1| alkaline amylopullulanase [Bacillus halodurans] pir||A84112 alkaline amylopullulanase BH3697 [imported] - Bacillus halodurans (strain C-125) dbj|BAB07416.1| alkaline amylopullulanase [Bacillus halodurans] Length = 1072 Score = 827 bits (2135), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/846 (51%), Positives = 559/846 (66%), Gaps = 28/846 (3%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVS 60 +DV L DA +IGFLVVNR +GDKDG DKTF L + QL+I+QGDD VY +P+ + Sbjct: 199 LDVELADDANEIGFLVVNRENGDKDGDDKTFADLANHPQLFIRQGDDTVYTNPYYQTIEG 258 Query: 61 LVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEVDTAA 120 LVS E++S ++ L FT T L L I +TD G V + I +V + + Sbjct: 259 LVSGELISLTEMELRFTSTIWLSEDDLAEQIHLTDTNGASVAFDHVTIHDEKTV-ILHGS 317 Query: 121 FALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASSV 180 F E AP V ++ K V A AGWR+ID +Y Y G+ LGA H D S+TL W+P+A V Sbjct: 318 FDAELAPYHVTFADKTVVARAGWRLIDALYGYDGE-LGAVLH-EDGSSTLTFWSPLADHV 375 Query: 181 VANVYSKTDAAQQV-GQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGV 239 +Y K D + + + +T GD+GVW L S+ +++ GYYY Y++ +G Sbjct: 376 SVVLYDKDDQYRIIKDDIDMTKGDQGVWQVTLH----SENTGLDNLAGYYYHYKIERDGD 431 Query: 240 TKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDA 299 TK LDPYAKSMA + + + + +GKAAIVD S P + +I G+EKREDA Sbjct: 432 TKLGLDPYAKSMAAWNNEGSYS-------IGKAAIVDPSSIGP-ELDFAEIEGFEKREDA 483 Query: 300 VIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDET 359 +IYEVHVRDFTSD IE EL + +G++A+F KLDYI+ LG+THIQL+PVM++Y GDE Sbjct: 484 IIYEVHVRDFTSDPGIEEELTAQ-FGTFASFVDKLDYIEDLGVTHIQLLPVMSYYWGDEL 542 Query: 360 RMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGV 419 RE YS+ N YNWGYDPHSYFS G YS+ P DPE RIKE K LID +H GMGV Sbjct: 543 ANHERELAYSSSGNNYNWGYDPHSYFSLSGMYSENPEDPELRIKEFKHLIDEIHRRGMGV 602 Query: 420 ILDVVYTHMAKKDFLDDIVPNYYAFQDAX-XXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 ILDVVY H A + +D++PNYY F DA L T+HKM+ +++VDS+ YW Sbjct: 603 ILDVVYNHTAAVEIFEDLMPNYYHFMDADGTPRTSFGGGRLGTTHKMSRRILVDSITYWV 662 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 EYK+DG R+DMMG +NP + IGEGW+T+ GD + AD Sbjct: 663 EEYKVDGFRFDMMGDHDAETIQIAYDRAKELNPNIVMIGEGWRTYVGDEHGGDIM--PAD 720 Query: 539 QAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDP 598 Q WM T+SVGVFSDEFRNELKSG+GSEG+PRFITGGAR+I IF+N+TA+P N A +P Sbjct: 721 QDWMQHTESVGVFSDEFRNELKSGFGSEGQPRFITGGARNIEQIFDNLTAQPHNFTATNP 780 Query: 599 GDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQ 658 GDVVPYIEAHDNLTLHDVIAQ+I+KDP ++ EI +RIRLGN + +T+QGTAF+ AGQ Sbjct: 781 GDVVPYIEAHDNLTLHDVIAQSIRKDPEY--HQEEIHKRIRLGNTMVLTAQGTAFLHAGQ 838 Query: 659 EYGRTKQWKA--AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVN 714 E+GRTKQ++A G K T M D NG+ F YFIHDSYDSSDA+N FDW KAT++ Sbjct: 839 EFGRTKQFRADTGGQAPYKSTYMTDENGKPFVYPYFIHDSYDSSDAINKFDWGKATNKEK 898 Query: 715 YPVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDG 774 YP+ + TR YT+GLI+LR+ST+AF G V NV L+ PEI TDLVIGY+ +TD Sbjct: 899 YPIHHLTRTYTAGLIELRRSTNAFTHGTLVDVQANVRLLDIPEISETDLVIGYRATSTDN 958 Query: 775 TGLYYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPL 834 TG YYVF+N D RTL+L++DLT G V+VD+D+AG+ A+ SGF LT IT+DPL Sbjct: 959 TGTYYVFVNADTKERTLSLTDDLTEGVVIVDSDEAGIVAV--SDPSGFDLTPNEITIDPL 1016 Query: 835 TAVVIR 840 TAV+IR Sbjct: 1017 TAVIIR 1022 Score = 57.0 bits (136), Expect = 5e-06, Method: Compositional matrix adjust. Identities = 29/62 (46%), Positives = 42/62 (67%), Gaps = 5/62 (8%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLD-RYNQLWIKQGDDHVYVSPFGEQPV 59 VD+ LK +A+ IGFLV+N ++GDKDG DK +LL ++WIK+G D V++ +PV Sbjct: 89 VDIELKENAENIGFLVLNTATGDKDGNDKHVQLLSPEMKEIWIKEGSDDVFLY----EPV 144 Query: 60 SL 61 L Sbjct: 145 DL 146 >dbj|BAB47586.1| alkaline pullulanase [Bacillus sp. KSM-1876] Length = 1142 Score = 808 bits (2086), Expect = 0.0, Method: Compositional matrix adjust. Identities = 426/845 (50%), Positives = 556/845 (65%), Gaps = 29/845 (3%) Query: 1 VDVPLKTDAQKIGFLVVNRSSGDKDGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGEQPVS 60 +D+PL A++IG +VVN+ + +++ D++F L+++NQ++I +GD VY +P+G Sbjct: 215 IDIPLNEGAEEIGLVVVNKETNEQEA-DRSFTQLNQFNQIFIVEGDGAVYSNPYGAIATE 273 Query: 61 LVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKS-TTSVEVDTA 119 L+SAE LS KI+L F+ TD L L + + D E V VN IK+ +VEV Sbjct: 274 LLSAEQLSDEKIVLYFSRTDELTEEALLEQVGIRDIEEHTVDVNEVEIKTDNRTVEV-KG 332 Query: 120 AFALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPMASS 179 F L+++P +V+ +V A GWR+IDEMY Y G+ LGA H D +AT+KLW+P A Sbjct: 333 DFNLDQSPYTVSLGEVSVPAKIGWRLIDEMYAYDGE-LGAQLH-QDGTATIKLWSPKAEH 390 Query: 180 VVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTNNGV 239 V +Y K + V +V + LGD+GVW +L + + +RGYYY YE+ ++G Sbjct: 391 VQVILYDKDNPDDIVTEVEMKLGDRGVWEVQLTKEN----TGLDSLRGYYYHYEIDHDGD 446 Query: 240 TKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKREDA 299 K LDPYAKS++ + D G KAAIVD S P + I G+EK+ED Sbjct: 447 KKIALDPYAKSLSAWNSDEQGP-------YAKAAIVDPSSIGP-ELEFAHIEGFEKKEDT 498 Query: 300 VIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNGDET 359 +IYEVHVRDFTSD I EL + +G++A+F KLDYI+ LG+THIQL+PVM+++ +E Sbjct: 499 IIYEVHVRDFTSDPHIADELTAQ-FGTFASFVDKLDYIEDLGVTHIQLLPVMSYFFANEF 557 Query: 360 RMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAGMGV 419 + R +S+ N YNWGYDP SYF+ G YS+ P DPE RIKE K LID +H GMGV Sbjct: 558 KNDERMLDFSSTNTNYNWGYDPQSYFALTGMYSEDPTDPELRIKEFKKLIDEIHSRGMGV 617 Query: 420 ILDVVYTHMAKKDFLDDIVPNYYAFQDAX-XXXXXXXXXXLATSHKMAEKLMVDSVKYWF 478 +LDVVY H A+ +D+VPNYY F DA L T+H+M+ +++VDS+ YW Sbjct: 618 VLDVVYNHTARVGIFEDLVPNYYHFMDADGTPRTSFGGGRLGTTHEMSRRILVDSITYWV 677 Query: 479 SEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGKGAD 538 E+KIDG R+DMMG INP + IGEGW T+AGD DP + + AD Sbjct: 678 EEFKIDGFRFDMMGDHDAESIQIAYDKAKEINPNIVMIGEGWITYAGDEDDPNV--QAAD 735 Query: 539 QAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPADDP 598 Q WM T+SVGVFSDEFRNELKSG+G EGEPRF+TGGAR+I IF+NI A+P N ADDP Sbjct: 736 QHWMQYTESVGVFSDEFRNELKSGFGHEGEPRFLTGGARNIDLIFDNIKAQPHNFIADDP 795 Query: 599 GDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQAGQ 658 GDVVPYIEAHDNLTLHDVIA I+KDP ++ EI QRIRLGNL+ +TSQG AF+ AGQ Sbjct: 796 GDVVPYIEAHDNLTLHDVIAMAIQKDPD--DHQEEIHQRIRLGNLMTLTSQGIAFLHAGQ 853 Query: 659 EYGRTKQWKA-AGVPEQKYTEMKDANGQSF--GYFIHDSYDSSDAVNMFDWAKATDEVNY 715 EYGRTKQ++A P K T M D NG+ F YFIHDSYDS+D +N FDW +AT+ Y Sbjct: 854 EYGRTKQFRAETSEPPYKSTYMTDENGEPFRYPYFIHDSYDSTDIINRFDWERATNADAY 913 Query: 716 PVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAPEIKATDLVIGYKNKATDGT 775 P+QN TR YT+GLI+LR+S+DAFRLG K LVD V + PEI+ TDLV+ Y+ +AT G Sbjct: 914 PIQNLTREYTTGLIELRRSSDAFRLGTKDLVDEKVTQLNIPEIEETDLVVAYRIEATTGE 973 Query: 776 GLYYVFMNGDDAARTLTLSEDLTGGQVLVDNDQAGVTAIPAESQSGFTLTSTSITLDPLT 835 +YVF+N DD RTLTL EDLT G+ +VD AGVTAI AE Q G L++ I LDPLT Sbjct: 974 A-FYVFVNADDEERTLTLEEDLTVGEFVVDGKTAGVTAI-AEPQ-GVELSAEQIKLDPLT 1030 Query: 836 AVVIR 840 A +++ Sbjct: 1031 AAIVQ 1035 >emb|CAD32942.1| pullulanase [Streptococcus pyogenes] Length = 1165 Score = 672 bits (1733), Expect = 0.0, Method: Compositional matrix adjust. Identities = 386/860 (44%), Positives = 514/860 (59%), Gaps = 42/860 (4%) Query: 1 VDVPLKTDAQKIGFLVVNRS-SGDK---DGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGE 56 VDVPLK A +IGFL++++S +GD D F+ LD + Q+++K D VY +P+ Sbjct: 250 VDVPLKEGANEIGFLILDKSKTGDAIKVQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYI 309 Query: 57 QPVSLVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEV 116 VSL AE + +I FT DGLD +K I +TDK G V ++ + SV Sbjct: 310 DQVSLKGAEQTTPNEIKAIFTTLDGLDEDAVKQNIKITDKAGKTVAIDELTLDKDKSVMT 369 Query: 117 DTAAFALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPM 176 F + A +V + + A W++ D++Y Y G+ LGAT D S L LW+P Sbjct: 370 LKGDFKAQGAVYTVTFGEVSQVARQSWQLKDKLYAYDGE-LGATL-AKDGSVDLALWSPS 427 Query: 177 ASSVVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTN 236 A SV VY K D + VGQ LT DKGVW A L SD K + D GYYY YE+T Sbjct: 428 ADSVKVVVYDKQDQTKVVGQADLTKSDKGVWRAHLT-SDSVKGIS--DYTGYYYLYEITR 484 Query: 237 NGVTKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKR 296 VLDPYAKS+A + A D KAA +D S P I ++KR Sbjct: 485 GQEKVMVLDPYAKSLAAWN----DATATDDIKTAKAAFIDPSKLGPTGLDFAKINNFKKR 540 Query: 297 EDAVIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNG 356 EDA+IYE HVRDFTSD ++E +L +G+++AF ++LDY+K LG+TH+QL+PV++++ Sbjct: 541 EDAIIYEAHVRDFTSDKALEGKLT-HPFGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYA 599 Query: 357 DETRMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAG 416 +E T R + Y++ +N YNWGYDP YF+ G YS P DP RI ELK L++ +H+ G Sbjct: 600 NELDKT-RSTAYTSSDNNYNWGYDPQHYFALSGMYSANPNDPALRIAELKNLVNEIHKRG 658 Query: 417 MGVILDVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXX-XLATSHKMAEKLMVDSVK 475 MGVI DVVY H A+ +D+ PNYY F +A L T+H M+ +++VDS+ Sbjct: 659 MGVIFDVVYNHTARTYLFEDLEPNYYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIT 718 Query: 476 YWFSEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGK 535 Y E+K+DG R+DMMG INP + IGEGW+T+ GD +A Sbjct: 719 YLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIA-- 776 Query: 536 GADQAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPA 595 ADQ WM T++VGVFSD+ RN LKSG+ +EG FITGGA+++ +F I A+PSN A Sbjct: 777 -ADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPSNFEA 835 Query: 596 DDPGDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQ 655 D PGDVV YI AHDNLTLHDVIA++I KDP + E EI +RIRLGN + +T+QGTAFI Sbjct: 836 DAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVA--EEEIHKRIRLGNTMILTAQGTAFIH 893 Query: 656 AGQEYGRTKQ-----WKAA----GVPEQKYTEMKDANGQSFGYFIHDSYDSSDAVNMFDW 706 +GQEYGRTKQ +K VP + + DA Q + YFIHDSYDSSDAVN FDW Sbjct: 894 SGQEYGRTKQLLNPDYKTKVSDDKVPNK--ATLIDAVAQ-YPYFIHDSYDSSDAVNHFDW 950 Query: 707 AKATDEVNYPVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAP---EIKATDL 763 AKATD + +P+ N T+AYT GLI LR+STDAF K+ VD +V LIT I+ DL Sbjct: 951 AKATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDL 1010 Query: 764 VIGYKNKATDGTGLYYVFMNGDDAARTLTLSED---LTGGQVLVDNDQAGVTAIPAESQS 820 ++GY+ A++G Y VF+N D+ R + L + L G QVL D +Q GVTAI Sbjct: 1011 IMGYQTVASNG-DRYAVFVNADNKTRKVVLPQAYRYLLGAQVLADAEQVGVTAI--AKPK 1067 Query: 821 GFTLTSTSITLDPLTAVVIR 840 G T +T+D LTA+V++ Sbjct: 1068 GVQFTKEGLTIDGLTALVLK 1087 >ref|NP_269940.1| putative pullulanase [Streptococcus pyogenes] gb|AAK34661.1| putative pullulanase [Streptococcus pyogenes M1 GAS] Length = 1165 Score = 671 bits (1732), Expect = 0.0, Method: Compositional matrix adjust. Identities = 383/858 (44%), Positives = 513/858 (59%), Gaps = 38/858 (4%) Query: 1 VDVPLKTDAQKIGFLVVNRS-SGDK---DGGDKTFRLLDRYNQLWIKQGDDHVYVSPFGE 56 VDVPLK A +IGFL++++S +GD D F+ LD + Q+++K D VY +P+ Sbjct: 250 VDVPLKEGANEIGFLILDKSKTGDAIKVQPKDYLFKELDNHTQVFVKDTDPKVYNNPYYI 309 Query: 57 QPVSLVSAEILSTRKILLTFTLTDGLDAAGLKSLITVTDKEGTPVPVNAAAIKSTTSVEV 116 VSL AE + +I FT DGLD +K I +TDK G V ++ + SV Sbjct: 310 DQVSLKGAEQTTPNEIKAIFTTLDGLDEDAVKQNIKITDKAGKTVAIDELTLDRDKSVMT 369 Query: 117 DTAAFALEKAPLSVAYSGKNVSASAGWRMIDEMYNYTGDDLGATYHPADQSATLKLWAPM 176 F + A +V + + A W++ D++Y Y G+ LGAT D S L LW+P Sbjct: 370 LKGDFKAQGAVYTVTFGEVSQVARQSWQLKDKLYAYDGE-LGATL-AKDGSVDLALWSPS 427 Query: 177 ASSVVANVYSKTDAAQQVGQVSLTLGDKGVWSAELKPSDLSKAAAAEDVRGYYYQYEVTN 236 A +V VY K D + VGQ LT DKGVW A L SD K + D GYYY YE+T Sbjct: 428 ADTVKVVVYDKQDQTRVVGQADLTKSDKGVWRAHL-TSDSVKGIS--DYTGYYYLYEITR 484 Query: 237 NGVTKPVLDPYAKSMAVFTVDSTGAAGPDGDTVGKAAIVDLSGTNPPDYRAGDIPGYEKR 296 VLDPYAKS+A + A D KAA +D S P I ++KR Sbjct: 485 GQEKVMVLDPYAKSLAAWN----DATATDDIKTAKAAFIDPSKLGPTGLDFAKINNFKKR 540 Query: 297 EDAVIYEVHVRDFTSDVSIESELGGERWGSYAAFAKKLDYIKSLGITHIQLMPVMAWYNG 356 EDA+IYE HVRDFTSD ++E +L +G+++AF ++LDY+K LG+TH+QL+PV++++ Sbjct: 541 EDAIIYEAHVRDFTSDKALEGKLT-HPFGTFSAFVEQLDYLKDLGVTHVQLLPVLSYFYA 599 Query: 357 DETRMTHRESGYSTQNNEYNWGYDPHSYFSPDGAYSQQPADPEERIKELKGLIDAVHEAG 416 +E + R + Y++ +N YNWGYDP YF+ G YS P DP RI ELK L++ +H+ G Sbjct: 600 NELDKS-RSTAYTSSDNNYNWGYDPQHYFALSGMYSANPNDPALRIAELKNLVNEIHKRG 658 Query: 417 MGVILDVVYTHMAKKDFLDDIVPNYYAFQDAXXXXXXXXXX-XLATSHKMAEKLMVDSVK 475 MGVI DVVY H A+ +D+ PNYY F +A L T+H M+ +++VDS+ Sbjct: 659 MGVIFDVVYNHTARTYLFEDLEPNYYHFMNADGTARESFGGGRLGTTHAMSRRILVDSIT 718 Query: 476 YWFSEYKIDGMRWDMMGXXXXXXXXXXXXXXXXINPKALFIGEGWKTFAGDASDPALAGK 535 Y E+K+DG R+DMMG INP + IGEGW+T+ GD +A Sbjct: 719 YLTREFKVDGFRFDMMGDHDAAAIEQAFKAAKAINPNTIMIGEGWRTYQGDEGKKEIA-- 776 Query: 536 GADQAWMDKTDSVGVFSDEFRNELKSGYGSEGEPRFITGGARSIAAIFNNITARPSNIPA 595 ADQ WM T++VGVFSD+ RN LKSG+ +EG FITGGA+++ +F I A+P N A Sbjct: 777 -ADQDWMKATNTVGVFSDDIRNTLKSGFPNEGTAAFITGGAKNLEGLFKTIKAQPGNFEA 835 Query: 596 DDPGDVVPYIEAHDNLTLHDVIAQTIKKDPSIPANELEIQQRIRLGNLLEMTSQGTAFIQ 655 D PGDVV YI AHDNLTLHDVIA++I KDP + E EI +RIRLGN + +T+QGTAFI Sbjct: 836 DAPGDVVQYIAAHDNLTLHDVIAKSINKDPKVA--EEEIHKRIRLGNTMILTAQGTAFIH 893 Query: 656 AGQEYGRTKQW-------KAAGVPEQKYTEMKDANGQSFGYFIHDSYDSSDAVNMFDWAK 708 +GQEYGRTKQ KA+ + DA Q + YFIHDSYDSSDAVN FDWAK Sbjct: 894 SGQEYGRTKQLLNPDYKTKASDDKVPNKATLIDAVAQ-YPYFIHDSYDSSDAVNHFDWAK 952 Query: 709 ATDEVNYPVQNTTRAYTSGLIQLRKSTDAFRLGDKSLVDTNVNLITAP---EIKATDLVI 765 ATD + +P+ N T+AYT GLI LR+STDAF K+ VD +V LIT I+ DL++ Sbjct: 953 ATDSIAHPISNQTKAYTQGLIALRRSTDAFTKATKAEVDRDVTLITQAGQDGIQQEDLIM 1012 Query: 766 GYKNKATDGTGLYYVFMNGDDAARTLTLSED---LTGGQVLVDNDQAGVTAIPAESQSGF 822 GY+ A++G Y VF+N D+ R + L + L G QVLVD +QAGVTAI G Sbjct: 1013 GYQTVASNG-DRYAVFVNADNKTRKVVLPQAYRYLLGAQVLVDAEQAGVTAIA--KPKGV 1069 Query: 823 TLTSTSITLDPLTAVVIR 840 T +T++ LTA+V++ Sbjct: 1070 QFTKEGLTIEGLTALVLK 1087 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.314 0.132 0.380 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 3,170,870,787 Number of extensions: 136897087 Number of successful extensions: 262023 Number of sequences better than 1.0e-05: 1140 Number of HSP's gapped: 259209 Number of HSP's successfully gapped: 1258 Length of query: 2165 Length of database: 757,237,578 Length adjustment: 141 Effective length of query: 2024 Effective length of database: 374,408,619 Effective search space: 757803044856 Effective search space used: 757803044856 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 134 (56.2 bits)