BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0291.0002.p (513 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00099328.1| COG2268: Uncharacterized protein conserved in... 696 0.0 ref|NP_390979.1| alternate gene name: yuaH~similar to epidermal ... 553 e-156 ref|NP_830375.1| Flottilin [Bacillus cereus ATCC 14579] >gi|2989... 490 e-137 ref|NP_843092.1| SPFH domain/band 7 family protein [Bacillus ant... 489 e-137 ref|NP_976944.1| SPFH domain/band 7 family protein [Bacillus cer... 488 e-136 >ref|ZP_00099328.1| COG2268: Uncharacterized protein conserved in bacteria [Desulfitobacterium hafniense] Length = 495 Score = 696 bits (1796), Expect = 0.0, Method: Compositional matrix adjust. Identities = 352/458 (76%), Positives = 407/458 (88%) Query: 25 FWARYKTVGPDEGMIVTGSFLGSNHISDDGSGRKIKIVRGGGAFILPVFQKAEFMSLLSH 84 FWARYKTVGPD+ MIVTGS+LG+ ++ D SGRKIKIVRGGGAFILPVFQ+A+F+SLLSH Sbjct: 22 FWARYKTVGPDQAMIVTGSYLGTKNVYTDESGRKIKIVRGGGAFILPVFQQAKFISLLSH 81 Query: 85 KLDVTTPEVYTEQGVPVIADGVAIIKVGSSTEDVANAAEQFMGKPIDALKSEAQEVLEGH 144 KLDVTTPEVYTEQGVPV+ADGVAIIK+G S EDVA AAEQF+ KP AL EAQEVLEGH Sbjct: 82 KLDVTTPEVYTEQGVPVMADGVAIIKIGGSVEDVATAAEQFLSKPAQALSQEAQEVLEGH 141 Query: 145 LRAILGSMTVEEVYRNRDRFAQEVQGVAARDLKKMGLQIVSFTIKDVRDKHGYLEALGKP 204 LRAILG MTVEEVYRNRD+FAQEVQG AA+DL+KMGLQIVSFTIKD+RDK+GYLEALGKP Sbjct: 142 LRAILGMMTVEEVYRNRDKFAQEVQGSAAKDLRKMGLQIVSFTIKDIRDKNGYLEALGKP 201 Query: 205 RIAAVKRDAEIAEAEALRDARIQKANAEEQGQKAELLRDTNIAEASKDNQLKVAAFKRDQ 264 RIA VKRDAE+AEAEA+RDARIQKA A E+GQKAELLRDT+IAEA+K+ +LKVA+FK++Q Sbjct: 202 RIAIVKRDAEVAEAEAVRDARIQKAKAAEEGQKAELLRDTSIAEATKEKELKVASFKKEQ 261 Query: 265 DTAKAEADQAYHIQEARAKQTVVEEQMKVELVRKEREIDLQAKEIQVREKQYDAEVKKKA 324 DTA AEADQAYHIQEAR++Q V EEQMKV LV+KE+EI++Q KEI REKQYDAEVKKKA Sbjct: 262 DTAMAEADQAYHIQEARSQQQVTEEQMKVTLVQKEKEIEIQEKEILRREKQYDAEVKKKA 321 Query: 325 EADRYAVEQAAEADKAKRMREADALQYSIETQARATSEQKRLEGQAVADAELAKGKAESE 384 +ADRYAVEQAAEADKAKRMREADAL+Y IE +A+A +EQKRL+G A+A+AE AKG AE+E Sbjct: 322 DADRYAVEQAAEADKAKRMREADALKYKIEAEAKANAEQKRLDGLAIAEAERAKGTAEAE 381 Query: 385 VIRLRGXXXXXXXXXXXXXFQKFGEAAVLDIIVKMLPELAGRIAEPLASIDKLTVVDTGN 444 V+RL+G F+K+G+AAVLDIIVKMLPELAG+IAEPL +IDKLTVVDTG Sbjct: 382 VVRLKGLAEAEAKEKLAEAFEKYGQAAVLDIIVKMLPELAGKIAEPLKAIDKLTVVDTGT 441 Query: 445 GEGAARVSNYVTQLMSTAPEMLKSVSGIDVEALIKGLT 482 G+GAA++SNYVT LM+TAPEML SVSGID++ L+K LT Sbjct: 442 GQGAAKLSNYVTTLMATAPEMLNSVSGIDLDKLVKQLT 479 >ref|NP_390979.1| alternate gene name: yuaH~similar to epidermal surface antigen [Bacillus subtilis] sp|O32076|YUAG_BACSU Hypothetical protein yuaG pir||A70006 epidermal surface antigen homolog yuaG - Bacillus subtilis emb|CAB15079.1| yuaG [Bacillus subtilis subsp. subtilis str. 168] Length = 509 Score = 553 bits (1425), Expect = e-156, Method: Compositional matrix adjust. Identities = 279/465 (60%), Positives = 374/465 (80%), Gaps = 4/465 (0%) Query: 25 FWARYKTVGPDEGMIVTGSFLGSNHISDDGSGRKIKIVRGGGAFILPVFQKAEFMSLLSH 84 F +Y+T GPDE +IVTGS+LG+ ++ D G +IKIVRGGG F+LPVFQ+AE +SLLS Sbjct: 23 FITKYRTAGPDEALIVTGSYLGNKNVHVDEGGNRIKIVRGGGTFVLPVFQQAEPLSLLSS 82 Query: 85 KLDVTTPEVYTEQGVPVIADGVAIIKVGSSTEDVANAAEQFMGKPIDALKSEAQEVLEGH 144 KLDV+TPEVYTEQGVPV+ADG AIIK+G S ++A AAEQF+GK D + EA+EVLEGH Sbjct: 83 KLDVSTPEVYTEQGVPVMADGTAIIKIGGSIGEIATAAEQFLGKSKDDREQEAREVLEGH 142 Query: 145 LRAILGSMTVEEVYRNRDRFAQEVQGVAARDLKKMGLQIVSFTIKDVRDKHGYLEALGKP 204 LR+ILGSMTVEE+Y+NR++F+QEVQ VA++DL KMGL IVSFTIKDVRDK+GYLE+LGKP Sbjct: 143 LRSILGSMTVEEIYKNREKFSQEVQRVASQDLAKMGLVIVSFTIKDVRDKNGYLESLGKP 202 Query: 205 RIAAVKRDAEIAEAEALRDARIQKANAEEQGQKAELLRDTNIAEASKDNQLKVAAFKRDQ 264 RIA VKRDA+IA AEA ++ RI++A A++ +K+EL R T IAEA K NQLK+A F+R+Q Sbjct: 203 RIAQVKRDADIATAEADKETRIKRAEADKDAKKSELERATEIAEAEKINQLKMAEFRREQ 262 Query: 265 DTAKAEADQAYHIQEARAKQTVVEEQMKVELVRKEREIDLQAKEIQVREKQYDAEVKKKA 324 DTAKA ADQAY ++ ARA+Q V E++M+V+++ ++++I+L+ KEI RE+QYD+EVKKKA Sbjct: 263 DTAKANADQAYDLETARARQQVTEQEMQVKIIERQKQIELEEKEILRRERQYDSEVKKKA 322 Query: 325 EADRYAVEQAAEADKAKRMREADALQYSIETQARATSEQKRLEGQAVADAELAKGKAESE 384 +ADRY+VEQ+A A+KAK++ EADA +YSIE A+A +E+ R++G A A+AE AKG+ E+E Sbjct: 323 DADRYSVEQSAAAEKAKQLAEADAKKYSIEAMAKAEAEKVRIDGLAKAEAEKAKGETEAE 382 Query: 385 VIRLRGXXXXXXXXXXXXXFQKFGEAAVLDIIVKMLPELAGRIAEPLASIDKLTVVDT-G 443 VIRL+G F+++G+AA+ D+IVKMLPE A + A PL++IDK+TVVDT G Sbjct: 383 VIRLKGLAEAEAKEKIAAAFEQYGQAAIFDMIVKMLPEYAKQAAAPLSNIDKITVVDTGG 442 Query: 444 NGE--GAARVSNYVTQLMSTAPEMLKSVSGIDVEALIKGLT-KGN 485 +GE GA +V++Y T LMS+ E LK+ SGIDV+ +++ + KGN Sbjct: 443 SGESSGANKVTSYATNLMSSLQESLKASSGIDVKEMLENFSGKGN 487 >ref|NP_830375.1| Flottilin [Bacillus cereus ATCC 14579] gb|AAP07576.1| Flottilin [Bacillus cereus ATCC 14579] Length = 524 Score = 490 bits (1262), Expect = e-137, Method: Compositional matrix adjust. Identities = 260/490 (53%), Positives = 368/490 (75%), Gaps = 18/490 (3%) Query: 25 FWARYKTVGPDEGMIVTGSFLGS--NHISDDGSGRKIKIVRGGGAFILPVFQKAEFMSLL 82 F +Y+TVGPDE +IVTG++LG N ++ D G+KIKI+RGGG F++P+ Q+AE +SLL Sbjct: 22 FITKYRTVGPDEALIVTGNWLGGGKNVVTTD-DGKKIKIIRGGGTFVVPIMQRAEPLSLL 80 Query: 83 SHKLDVTTPEVYTEQGVPVIADGVAIIKVGSSTEDVANAAEQFMGKPIDALKSEAQEVLE 142 ++KL+V T + YT+QGVPV +GV+IIKVGS+ E+V+ AAEQ++GK + LK EA+EVLE Sbjct: 81 NYKLEVGTRDTYTKQGVPVTVNGVSIIKVGSTIEEVSTAAEQYLGKETEELKVEAKEVLE 140 Query: 143 GHLRAILGSMTVEEVYRNRDRFAQEVQGVAARDLKKMGLQIVSFTIKDVRDKHGYLEALG 202 GHLRAIL SMTVE+ Y NR++FAQ+V VA+ DLKKMGL+IVSFTIK++ DK+GYL+ALG Sbjct: 141 GHLRAILSSMTVEDAYSNREQFAQKVHEVASTDLKKMGLRIVSFTIKEIMDKNGYLDALG 200 Query: 203 KPRIAAVKRDAEIAEAEALRDARIQKANAEEQGQKAELLRDTNIAEASKDNQLKVAAFKR 262 +P+IA VKRDA IA AE ++ARI+KA AE++ ++AE RD IAEA K +LKV ++KR Sbjct: 201 QPQIATVKRDATIANAEREKEARIEKARAEKEAKEAEYQRDAQIAEAEKHKELKVQSYKR 260 Query: 263 DQDTAKAEADQAYHIQEARAKQTVVEEQMKVELVRKEREIDLQAKEIQVREKQYDAEVKK 322 +Q+ A+A+AD +Y +Q+A+A+Q V EEQM+V+++ +E++I+L+ KEI REKQYDAEVKK Sbjct: 261 EQEQARADADLSYELQQAKAQQGVTEEQMRVKIIEREKQIELEEKEIARREKQYDAEVKK 320 Query: 323 KAEADRYAVEQAAEADKAKRMREADALQYSIETQAR-----------ATSEQKRLEGQAV 371 KA+ADRYAVEQ+AEA+K K++++ADA QY IE +AR A +E ++ +GQA Sbjct: 321 KADADRYAVEQSAEAEKVKQIKKADADQYKIEAEARARAEEVRVEGLAKAEIEKAQGQAK 380 Query: 372 ADAELAKGKAESEVIRLRGXXXXXXXXXXXXXFQKFGEAAVLDIIVKMLPELAGRIAEPL 431 A+ + A+G AE++VIRL+G F+ +G+AA++D+++KMLP A +A PL Sbjct: 381 AEVQKAQGTAEADVIRLKGLAEAEAKQKIAEAFELYGQAAIMDMVLKMLPSYAKEVASPL 440 Query: 432 ASIDKLTVVDT---GNGEGAARVSNYVTQLMSTAPEMLKSVSGIDVEALIKGLT-KGNTH 487 ++IDK+TVVDT G GA +V+ Y T LM+T E LK+ SGID++ L++G KG Sbjct: 441 SNIDKITVVDTGGGGKNSGAGKVAGYATDLMATMQETLKASSGIDLKELLEGFAGKGAVQ 500 Query: 488 TPASSGPAVT 497 +S P V+ Sbjct: 501 QLSSDKPVVS 510 >ref|NP_843092.1| SPFH domain/band 7 family protein [Bacillus anthracis str. Ames] gb|AAP24578.1| SPFH domain/band 7 family protein [Bacillus anthracis str. Ames] Length = 526 Score = 489 bits (1260), Expect = e-137, Method: Compositional matrix adjust. Identities = 259/490 (52%), Positives = 368/490 (75%), Gaps = 18/490 (3%) Query: 25 FWARYKTVGPDEGMIVTGSFLGS--NHISDDGSGRKIKIVRGGGAFILPVFQKAEFMSLL 82 F +Y+TVGPDE +IVTG++LG N ++ D G+KIKI+RGGG F++P+ Q+AE +SLL Sbjct: 22 FITKYRTVGPDEALIVTGNWLGGGKNVVTTD-DGKKIKIIRGGGTFVVPIMQRAEPLSLL 80 Query: 83 SHKLDVTTPEVYTEQGVPVIADGVAIIKVGSSTEDVANAAEQFMGKPIDALKSEAQEVLE 142 ++KL+V T + YT+QGVPV +GV+IIKVGS+ E+V+ AAEQ++GK + LK EA+EVLE Sbjct: 81 NYKLEVGTRDTYTKQGVPVTVNGVSIIKVGSTIEEVSTAAEQYLGKETEELKVEAKEVLE 140 Query: 143 GHLRAILGSMTVEEVYRNRDRFAQEVQGVAARDLKKMGLQIVSFTIKDVRDKHGYLEALG 202 GHLRAIL SMTVE+ Y NR++FAQ+V VA+ DLKKMGL+IVSFTIK++ DK+GYL+ALG Sbjct: 141 GHLRAILSSMTVEDAYSNREQFAQKVHEVASTDLKKMGLRIVSFTIKEIMDKNGYLDALG 200 Query: 203 KPRIAAVKRDAEIAEAEALRDARIQKANAEEQGQKAELLRDTNIAEASKDNQLKVAAFKR 262 +P+IA VKRDA IA AE ++ARI+KA AE++ ++AE RD IAEA K +LKV ++KR Sbjct: 201 QPQIATVKRDATIANAEREKEARIEKARAEKEAKEAEYQRDAQIAEAEKHKELKVQSYKR 260 Query: 263 DQDTAKAEADQAYHIQEARAKQTVVEEQMKVELVRKEREIDLQAKEIQVREKQYDAEVKK 322 +Q+ A+A+AD +Y +Q+A+A+Q V EEQM+V+++ +E++I+L+ KEI REKQYDAEVKK Sbjct: 261 EQEQARADADLSYELQQAKAQQGVTEEQMRVKIIEREKQIELEEKEIARREKQYDAEVKK 320 Query: 323 KAEADRYAVEQAAEADKAKRMREADALQYSIETQAR-----------ATSEQKRLEGQAV 371 KA+ADRYAVEQ+AEA+K K++++ADA QY IE +AR A +E ++ +GQA Sbjct: 321 KADADRYAVEQSAEAEKVKQIKKADADQYKIEAEARARAEEVRVEGLAKAEIEKAQGQAK 380 Query: 372 ADAELAKGKAESEVIRLRGXXXXXXXXXXXXXFQKFGEAAVLDIIVKMLPELAGRIAEPL 431 A+ + A+G AE++VIRL+G F+ +G+AA++D+++KMLP A +A PL Sbjct: 381 AEVQKAQGTAEADVIRLKGLAEAEAKQKIAEAFELYGQAAIMDMVLKMLPSYAKEVASPL 440 Query: 432 ASIDKLTVVDT---GNGEGAARVSNYVTQLMSTAPEMLKSVSGIDVEALIKGLT-KGNTH 487 ++IDK+TVVDT G GA +++ Y T LM+T E LK+ SGID++ L++G KG Sbjct: 441 SNIDKITVVDTGGGGKNSGAGKIAGYATDLMATMQETLKASSGIDLKELLEGFAGKGAVQ 500 Query: 488 TPASSGPAVT 497 +S P V+ Sbjct: 501 QLSSDKPVVS 510 >ref|NP_976944.1| SPFH domain/band 7 family protein [Bacillus cereus ATCC 10987] gb|AAS39552.1| SPFH domain/band 7 family protein [Bacillus cereus ATCC 10987] Length = 524 Score = 488 bits (1257), Expect = e-136, Method: Compositional matrix adjust. Identities = 259/490 (52%), Positives = 368/490 (75%), Gaps = 18/490 (3%) Query: 25 FWARYKTVGPDEGMIVTGSFLGS--NHISDDGSGRKIKIVRGGGAFILPVFQKAEFMSLL 82 F +Y+TVGPDE +IVTG++LG N ++ D G+KIKI+RGGG F++P+ Q+AE +SLL Sbjct: 22 FITKYRTVGPDEALIVTGNWLGGGKNVVTTD-DGKKIKIIRGGGTFVVPIMQRAEPLSLL 80 Query: 83 SHKLDVTTPEVYTEQGVPVIADGVAIIKVGSSTEDVANAAEQFMGKPIDALKSEAQEVLE 142 ++KL+V T + YT+QGVPV +GV+IIKVGS+ E+V+ AAEQ++GK + LK EA+EVLE Sbjct: 81 NYKLEVGTRDTYTKQGVPVTVNGVSIIKVGSTIEEVSTAAEQYLGKETEELKVEAKEVLE 140 Query: 143 GHLRAILGSMTVEEVYRNRDRFAQEVQGVAARDLKKMGLQIVSFTIKDVRDKHGYLEALG 202 GHLRAIL SMTVE+ Y NR++FAQ+V VA+ DLKKMGL+IVSFTIK++ DK+GYL+ALG Sbjct: 141 GHLRAILSSMTVEDAYSNREQFAQKVHEVASTDLKKMGLRIVSFTIKEIMDKNGYLDALG 200 Query: 203 KPRIAAVKRDAEIAEAEALRDARIQKANAEEQGQKAELLRDTNIAEASKDNQLKVAAFKR 262 +P+IA VKRDA IA AE ++ARI+KA AE++ ++AE RD IAEA K +LKV ++KR Sbjct: 201 QPQIATVKRDATIANAEREKEARIEKARAEKEAKEAEYQRDAQIAEAEKHKELKVQSYKR 260 Query: 263 DQDTAKAEADQAYHIQEARAKQTVVEEQMKVELVRKEREIDLQAKEIQVREKQYDAEVKK 322 +Q+ A+A+AD +Y +Q+A+A+Q V EEQM+V+++ +E++I+L+ KEI REKQYDAEVKK Sbjct: 261 EQEQARADADLSYELQQAKAQQGVTEEQMRVKIIEREKQIELEEKEIARREKQYDAEVKK 320 Query: 323 KAEADRYAVEQAAEADKAKRMREADALQYSIETQAR-----------ATSEQKRLEGQAV 371 KA+ADRYAVEQ+AEA+K K++++ADA QY IE +AR A +E ++ +GQA Sbjct: 321 KADADRYAVEQSAEAEKVKQIKKADADQYKIEAEARARAEEVRVEGLAKAEIEKAQGQAK 380 Query: 372 ADAELAKGKAESEVIRLRGXXXXXXXXXXXXXFQKFGEAAVLDIIVKMLPELAGRIAEPL 431 A+ + A+G AE++VIRL+G F+ +G+AA++D+++KMLP A +A PL Sbjct: 381 AEVQKAQGTAEADVIRLKGLAEAEAKQKIAEAFELYGQAAIMDMVLKMLPSYAKEVASPL 440 Query: 432 ASIDKLTVVDT---GNGEGAARVSNYVTQLMSTAPEMLKSVSGIDVEALIKGLT-KGNTH 487 ++IDK+TVVDT G GA +V+ Y T LM+T E LK+ SGID++ L++G KG Sbjct: 441 SNIDKITVVDTGGGGKNSGAGKVAGYATDLMATMQETLKASSGIDLKELLEGFAGKGAVQ 500 Query: 488 TPASSGPAVT 497 ++ P V+ Sbjct: 501 QLSNDKPIVS 510 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.313 0.129 0.342 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 613,373,661 Number of extensions: 23659026 Number of successful extensions: 107380 Number of sequences better than 1.0e-05: 325 Number of HSP's gapped: 105906 Number of HSP's successfully gapped: 473 Length of query: 513 Length of database: 757,237,578 Length adjustment: 130 Effective length of query: 383 Effective length of database: 404,274,708 Effective search space: 154837213164 Effective search space used: 154837213164 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 128 (53.9 bits)