BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0070.p (652 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value gb|AAD09354.1| s-layer associated multidomain endoglucanase [The... 102 3e-20 ref|ZP_00060727.1| hypothetical protein [Clostridium thermocellu... 94 9e-18 ref|ZP_00061549.1| COG2730: Endoglucanase [Clostridium thermocel... 92 5e-17 ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellu... 92 6e-17 dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] 90 2e-16 >gb|AAD09354.1| s-layer associated multidomain endoglucanase [Thermoanaerobacterium polysaccharolyticum] Length = 1097 Score = 102 bits (255), Expect = 3e-20, Method: Compositional matrix adjust. Identities = 63/181 (34%), Positives = 95/181 (52%), Gaps = 9/181 (4%) Query: 31 FSDINNSYAKEAILELLDKGIISGIGDNRFDPAGQLKRQDFAVILAKALSLDTTTVPSVP 90 F+DI++S+AK I L K IISG D F P ++ R +F +L KAL + ++P VP Sbjct: 913 FTDISSSWAKNEIQVLASKNIISGYPDGTFKPDKRITRAEFVSMLVKALGI-KQSIPDVP 971 Query: 91 TFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGIEASDK-- 148 TF+DV K + Y VEA G +G G +F ++RQ+M+V+ V AL + +K Sbjct: 972 TFSDVNKGDWYYGLVEAAKSTGIASGYGK-QFKPDMQITRQEMMVMVVNALRVNKVEKFV 1030 Query: 149 -----SSFLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKF 203 S F D K+ ++AK ++A + GLI+G G +P A R A V + Sbjct: 1031 SKGDVSVLGKFKDGGKVQNWAKDAMAIGVSNGLIKGTGDEYLSPDGRATRAQAAAVIYRM 1090 Query: 204 I 204 + Sbjct: 1091 L 1091 >ref|ZP_00060727.1| hypothetical protein [Clostridium thermocellum ATCC 27405] Length = 2547 Score = 94.4 bits (233), Expect = 9e-18, Method: Compositional matrix adjust. Identities = 59/181 (32%), Positives = 92/181 (50%), Gaps = 9/181 (4%) Query: 31 FSDINN-SYAKEAILELLDKGIISGIGDNRFDPAGQLKRQDFAVILAKALSLDTTTVPSV 89 F D+ +AKE I L KGII+G ++ P + R D +++ KAL L+ + Sbjct: 2363 FDDLKGYDWAKEQIEILASKGIINGTSSTKYSPGLNITRADAVILIVKALGLEAEFTEN- 2421 Query: 90 PTFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGI-----E 144 F DV + Y Y AV G + G GN +F +P++RQ+++VI RAL + + Sbjct: 2422 --FDDVSADKYYYEAVGIARSFGIVTGVGNNKFNPETPITRQELMVIVNRALKVVGINLD 2479 Query: 145 ASDKSSFLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKFI 204 D S F D ++I+ YA SVAA ++ G+I+G+ L P N R A++ K Sbjct: 2480 TGDVSELEAFKDFSEISPYAVESVAALVKAGIIKGDDNKLIAPLRNITRAETAVIIYKLF 2539 Query: 205 K 205 + Sbjct: 2540 E 2540 Score = 62.4 bits (150), Expect = 4e-08, Method: Compositional matrix adjust. Identities = 33/116 (28%), Positives = 60/116 (51%), Gaps = 4/116 (3%) Query: 91 TFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGIEASDKSS 150 +F D+ +A +E + G ING + +++ G ++R D +++ V+ALG+EA + Sbjct: 2362 SFDDLKGYDWAKEQIEILASKGIINGTSSTKYSPGLNITRADAVILIVKALGLEAEFTEN 2421 Query: 151 FLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKFIKV 206 F D Y +V A +G++ G G N FNP RQ + ++ ++ +KV Sbjct: 2422 F----DDVSADKYYYEAVGIARSFGIVTGVGNNKFNPETPITRQELMVIVNRALKV 2473 >ref|ZP_00061549.1| COG2730: Endoglucanase [Clostridium thermocellum ATCC 27405] Length = 1013 Score = 92.0 bits (227), Expect = 5e-17, Method: Compositional matrix adjust. Identities = 61/182 (33%), Positives = 91/182 (50%), Gaps = 11/182 (6%) Query: 31 FSDINNSY-AKEAILELLDKGIISGIGDNRFDPAGQLKRQDFAVILAKALSLDTTTVPSV 89 F D+ Y A+E I +L +KGIISGI + F P + R +F I+ +L + Sbjct: 835 FDDVPRGYWAEEYIQKLYEKGIISGIDEKTFMPGRPITRAEFTQIIVNSLKIPYREAGL- 893 Query: 90 PTFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRAL------GI 143 F DV + + Y +V + G + G +G FA ++RQDM V+ + L + Sbjct: 894 -HFNDVTEKDWYYKSVSSAAAFGIVVGRPDGSFAPNEFITRQDMAVVIAKFLEKKHDGNL 952 Query: 144 EASDKSSFLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKF 203 E K L FADS I++YA+ SVAA + GL+ G+ GN+F+P R V K Sbjct: 953 EGMGKG--LVFADSGNISEYARDSVAAVVSQGLMVGKPGNMFDPKGLTTRAEAVTVICKL 1010 Query: 204 IK 205 +K Sbjct: 1011 MK 1012 Score = 57.8 bits (138), Expect = 9e-07, Method: Compositional matrix adjust. Identities = 35/117 (29%), Positives = 60/117 (51%), Gaps = 3/117 (2%) Query: 92 FTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGIEASDKSSF 151 F DVP+ +A ++ +Y+ G I+G F G P++R + I V +L I + + Sbjct: 835 FDDVPRGYWAEEYIQKLYEKGIISGIDEKTFMPGRPITRAEFTQIIVNSLKIPY--REAG 892 Query: 152 LPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKFIKVKE 208 L F D + D+ SV++A +G++ G F P RQ +A+V +KF++ K Sbjct: 893 LHFNDVTE-KDWYYKSVSSAAAFGIVVGRPDGSFAPNEFITRQDMAVVIAKFLEKKH 948 >ref|ZP_00060097.1| hypothetical protein [Clostridium thermocellum ATCC 27405] pir||JC6031 scaffoldin dockerin binding protein precursor - Clostridium thermocellum gb|AAB07763.1| SdbA Length = 631 Score = 91.7 bits (226), Expect = 6e-17, Method: Compositional matrix adjust. Identities = 62/179 (34%), Positives = 92/179 (51%), Gaps = 10/179 (5%) Query: 31 FSDINN-SYAKEAILELLDKGIISGIGDNRFDPAGQLKRQDFAVILAKALSLDTTTVPSV 89 F+DI + ++AK+ I L KG+I+G D F P + R DF ++L KAL L T V S Sbjct: 454 FADIGSYAWAKKQIEVLASKGVINGTSDTTFTPQADITRADFMILLVKALGL-TAEVTS- 511 Query: 90 PTFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGIEAS--- 146 F DV + Y Y V + G G GN +F + ++RQDM+V+ AL I Sbjct: 512 -NFDDVSEKDYYYEYVGIAKELGITTGVGNNKFNPKAKITRQDMMVLTTNALRIAGKISS 570 Query: 147 --DKSSFLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKF 203 ++ F+D +IA YA VA ++ G++ G G++ NP NA R +A + K Sbjct: 571 TGTRADVERFSDKDQIASYAVEGVATLVKEGIVVG-SGDIINPRGNASRAELAAIIYKI 628 Score = 62.4 bits (150), Expect = 3e-08, Method: Compositional matrix adjust. Identities = 35/116 (30%), Positives = 58/116 (50%), Gaps = 4/116 (3%) Query: 91 TFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGIEASDKSS 150 TF D+ ++A +E + G ING + F + ++R D +++ V+ALG+ A S+ Sbjct: 453 TFADIGSYAWAKKQIEVLASKGVINGTSDTTFTPQADITRADFMILLVKALGLTAEVTSN 512 Query: 151 FLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKFIKV 206 F D DY V A E G+ G G N FNP A RQ + ++ + +++ Sbjct: 513 F----DDVSEKDYYYEYVGIAKELGITTGVGNNKFNPKAKITRQDMMVLTTNALRI 564 >dbj|BAC45001.1| xylanase 5 [Paenibacillus sp. W-61] Length = 1326 Score = 89.7 bits (221), Expect = 2e-16, Method: Compositional matrix adjust. Identities = 58/177 (32%), Positives = 94/177 (53%), Gaps = 6/177 (3%) Query: 31 FSDINNS-YAKEAILELLDKGIISGIGDNRFDPAGQLKRQDFAVILAKALSLDTTTVPSV 89 F+D+ + +AKEAI + + II GI D F PA + R DF +L +AL L + Sbjct: 1149 FADVQHVLWAKEAIEAMAARDIIKGISDESFAPAASITRADFITLLVRALELKGKDT-NA 1207 Query: 90 PTFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRAL---GIEAS 146 F+DV +Y AV + G +G + F GS +SRQDM+V+ +AL G ++ Sbjct: 1208 AAFSDVQSTAYYAQAVAIAKELGIASGFEDNTFKPGSSISRQDMMVLTAKALKAAGKPST 1267 Query: 147 DKSSFLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKF 203 + P++D+A I+ YA SV + + G++ G+GG + P + R A++ S+ Sbjct: 1268 GSGNLAPYSDAASISTYAVDSVTSLVGSGIVNGKGGKIA-PTESLTRAEAAVILSRI 1323 Score = 57.8 bits (138), Expect = 9e-07, Method: Compositional matrix adjust. Identities = 38/115 (33%), Positives = 61/115 (53%), Gaps = 2/115 (1%) Query: 91 TFTDVPKNSYAYNAVEAVYQAGWINGPGNGRFAAGSPLSRQDMIVIFVRALGIEASDKSS 150 +F DV +A A+EA+ I G + FA + ++R D I + VRAL ++ D ++ Sbjct: 1148 SFADVQHVLWAKEAIEAMAARDIIKGISDESFAPAASITRADFITLLVRALELKGKDTNA 1207 Query: 151 FLPFADSAKIADYAKASVAAALEYGLIQGEGGNLFNPAANADRQSVALVASKFIK 205 F+D A YA+A VA A E G+ G N F P ++ RQ + ++ +K +K Sbjct: 1208 -AAFSDVQSTAYYAQA-VAIAKELGIASGFEDNTFKPGSSISRQDMMVLTAKALK 1260 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.311 0.129 0.362 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 840,447,782 Number of extensions: 34639094 Number of successful extensions: 71035 Number of sequences better than 1.0e-05: 116 Number of HSP's gapped: 70863 Number of HSP's successfully gapped: 131 Length of query: 652 Length of database: 757,237,578 Length adjustment: 132 Effective length of query: 520 Effective length of database: 398,844,510 Effective search space: 207399145200 Effective search space used: 207399145200 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 129 (54.3 bits)