BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0058.p (196 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|ZP_00103230.1| COG0576: Molecular chaperone GrpE (heat shock... 158 6e-38 dbj|BAA90472.1| GrpE [Brevibacillus choshinensis] 141 7e-33 ref|NP_242211.1| heat-shock protein (activation of DnaK) [Bacill... 138 6e-32 dbj|BAB03214.1| grpE [Geobacillus thermoglucosidasius] 129 3e-29 ref|NP_927140.1| heat shock protein [Gloeobacter violaceus] >gi|... 127 1e-28 >ref|ZP_00103230.1| COG0576: Molecular chaperone GrpE (heat shock protein) [Desulfitobacterium hafniense] Length = 217 Score = 158 bits (400), Expect = 6e-38, Method: Compositional matrix adjust. Identities = 73/138 (52%), Positives = 101/138 (73%) Query: 58 ADEYQGRVLRVQADFDNFRRRTQKEKEELAQYATSKLVGELLPVLDNFERAIATVPATPE 117 A+EY + R+QA+FDN+R+RTQKEKE+ A+YA+ ++V LLPVLDNFERA+ T + Sbjct: 79 AEEYYTHLQRLQAEFDNYRKRTQKEKEDFAKYASERVVEGLLPVLDNFERAVEASKTTQD 138 Query: 118 FEAFNKGVNMIFQQLEGVLKSEGLAAMETVGQPFNPEYHQAIMQXXXXXXXXXXXXXXXQ 177 ++F++GV MIF+QL+G+L EGLAA+E VGQPF+P H+A++Q Q Sbjct: 139 MKSFSQGVEMIFKQLQGILAKEGLAAIEAVGQPFDPNLHEAVLQVDSEDYPESTVVEELQ 198 Query: 178 KGYLLKDKVLRPAMVKVS 195 KGY LK+KV+RP+MVKVS Sbjct: 199 KGYYLKEKVIRPSMVKVS 216 >dbj|BAA90472.1| GrpE [Brevibacillus choshinensis] Length = 179 Score = 141 bits (356), Expect = 7e-33, Method: Compositional matrix adjust. Identities = 67/138 (48%), Positives = 93/138 (67%) Query: 58 ADEYQGRVLRVQADFDNFRRRTQKEKEELAQYATSKLVGELLPVLDNFERAIATVPATPE 117 A+E+Q R+LR AD +N RRR +KE+E+LA+YA+ K+V ELLP+LDNFERA+A + Sbjct: 41 AEEHQNRMLRTMADMENLRRRVRKEQEDLAKYASQKVVEELLPILDNFERALAADKESMT 100 Query: 118 FEAFNKGVNMIFQQLEGVLKSEGLAAMETVGQPFNPEYHQAIMQXXXXXXXXXXXXXXXQ 177 E+ GV+M+++Q+ V EGL A+ GQPF+P HQA+MQ Q Sbjct: 101 VESLLTGVDMVYRQMVQVFDKEGLVAIAAKGQPFDPHVHQAVMQTQDPAFESGVVVEELQ 160 Query: 178 KGYLLKDKVLRPAMVKVS 195 KGY+ KD+V+RPAMVKV+ Sbjct: 161 KGYMFKDRVVRPAMVKVN 178 >ref|NP_242211.1| heat-shock protein (activation of DnaK) [Bacillus halodurans] sp|Q9KD73|GRPE_BACHD GrpE protein (HSP-70 cofactor) pir||A83818 heat-shock protein (activation of DnaK) grpE [imported] - Bacillus halodurans (strain C-125) dbj|BAB05064.1| heat-shock protein (activation of DnaK) [Bacillus halodurans] Length = 194 Score = 138 bits (348), Expect = 6e-32, Method: Compositional matrix adjust. Identities = 70/147 (47%), Positives = 93/147 (63%), Gaps = 3/147 (2%) Query: 52 KRLQEL---ADEYQGRVLRVQADFDNFRRRTQKEKEELAQYATSKLVGELLPVLDNFERA 108 KR+ EL DE R+LR+QAD+DNFRRR ++EKE A+Y L+ ELLP LDNFERA Sbjct: 47 KRIAELEGQVDELNQRLLRIQADYDNFRRRQREEKEAAAKYRAQSLIEELLPALDNFERA 106 Query: 109 IATVPATPEFEAFNKGVNMIFQQLEGVLKSEGLAAMETVGQPFNPEYHQAIMQXXXXXXX 168 + P E + KG+ M+++Q+ LK EGL +ET G+ F+P HQA+MQ Sbjct: 107 LLVEPEQEETKTLLKGMEMVYRQVSEALKKEGLEVIETKGETFDPHLHQAVMQVEDAEFE 166 Query: 169 XXXXXXXXQKGYLLKDKVLRPAMVKVS 195 QKGY LKD+V+RP+MVKV+ Sbjct: 167 SNEIVEELQKGYKLKDRVIRPSMVKVN 193 >dbj|BAB03214.1| grpE [Geobacillus thermoglucosidasius] Length = 224 Score = 129 bits (324), Expect = 3e-29, Method: Compositional matrix adjust. Identities = 63/136 (46%), Positives = 87/136 (63%) Query: 60 EYQGRVLRVQADFDNFRRRTQKEKEELAQYATSKLVGELLPVLDNFERAIATVPATPEFE 119 E + R LR+ ADF+NFRRRT++E E +Y LV +LLPVLDNFERA+ + + Sbjct: 88 EMENRYLRLYADFENFRRRTRREMEAAEKYRAQSLVSDLLPVLDNFERALKIKAEDEQAK 147 Query: 120 AFNKGVNMIFQQLEGVLKSEGLAAMETVGQPFNPEYHQAIMQXXXXXXXXXXXXXXXQKG 179 + +G+ M+++ + LK EG+ A+E VG+PF+P HQA+MQ QKG Sbjct: 148 SILQGMEMVYRSVLDALKKEGVEAIEAVGKPFDPHLHQAVMQVEDSNYEPNTVVEELQKG 207 Query: 180 YLLKDKVLRPAMVKVS 195 Y LKD+V+RPAMVKVS Sbjct: 208 YKLKDRVIRPAMVKVS 223 >ref|NP_927140.1| heat shock protein [Gloeobacter violaceus] dbj|BAC92135.1| heat shock protein [Gloeobacter violaceus PCC 7421] Length = 196 Score = 127 bits (320), Expect = 1e-28, Method: Compositional matrix adjust. Identities = 64/144 (44%), Positives = 92/144 (63%), Gaps = 1/144 (0%) Query: 54 LQELADEYQGRVLRVQADFDNFRRRTQKEKEELAQYATSKLVGELLPVLDNFERAIATV- 112 LQ+ +Y+ + R+ ADFDNFR+RTQ+EK+ELA + ++KL+ ++LPV DNF+RA A Sbjct: 48 LQKKLADYEQKYTRLMADFDNFRKRTQREKDELAYFVSAKLLKDILPVFDNFDRARAFAQ 107 Query: 113 PATPEFEAFNKGVNMIFQQLEGVLKSEGLAAMETVGQPFNPEYHQAIMQXXXXXXXXXXX 172 P E + +++Q VL+ G+ AME +GQPF+P H+AI++ Sbjct: 108 PDNEREEKLHNSYQQVYRQFLSVLEKMGVTAMEAIGQPFDPAQHEAILREESAGVSQETV 167 Query: 173 XXXXQKGYLLKDKVLRPAMVKVSM 196 QKGYLL DKVLRPAMVKV++ Sbjct: 168 VAELQKGYLLADKVLRPAMVKVAI 191 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.315 0.132 0.355 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 205,816,927 Number of extensions: 6695584 Number of successful extensions: 19445 Number of sequences better than 1.0e-05: 584 Number of HSP's gapped: 18829 Number of HSP's successfully gapped: 584 Length of query: 196 Length of database: 757,237,578 Length adjustment: 119 Effective length of query: 77 Effective length of database: 434,140,797 Effective search space: 33428841369 Effective search space used: 33428841369 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 122 (51.6 bits)