BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0046.p (344 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_980695.1| spore protease [Bacillus cereus ATCC 10987] >gi... 372 e-101 ref|NP_834031.1| Spore protease [Bacillus cereus ATCC 14579] >gi... 362 5e-99 ref|NP_242206.1| spore protease (degradation of SASPs) [Bacillus... 360 2e-98 ref|NP_658353.1| Ca_channel_B, Dihydropyridine sensitive L-type ... 358 8e-98 ref|NP_390432.1| spore protease [Bacillus subtilis] >gi|121563|s... 351 1e-95 >ref|NP_980695.1| spore protease [Bacillus cereus ATCC 10987] gb|AAS43303.1| spore protease [Bacillus cereus ATCC 10987] Length = 367 Score = 372 bits (954), Expect = e-101, Method: Compositional matrix adjust. Identities = 188/364 (51%), Positives = 258/364 (70%), Gaps = 31/364 (8%) Query: 11 LDLQLYSVRTDLALEAKEMAQGPQKTP--IPGVNEEVEEADGIKVTRLAVANDAGSQAIG 68 LDL YSVRTDLA+EA +M Q Q+ I GV + E +GI VT++ + ++A S ++G Sbjct: 5 LDLSKYSVRTDLAVEAHQMLQERQEEQKGIEGVIVKEREEEGITVTKVTI-DEAASDSMG 63 Query: 69 RAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVTP 128 + GNY+TLEV +R DT LQQKV +FA+EF F++ +G+ + AS LIVGLGNWNVTP Sbjct: 64 KKPGNYLTLEVQGIRQQDTELQQKVERIFAKEFSYFLEEVGVTKEASCLIVGLGNWNVTP 123 Query: 129 DSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTKP 188 D+LGP+VVEN L+TR ++L P+ V G+R VSAI PGV+G+TGIE+S+V+ GII++TKP Sbjct: 124 DALGPIVVENVLVTRHLFQLQPESVEEGFRPVSAIRPGVMGITGIETSDVIYGIIEKTKP 183 Query: 189 DVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVCY 248 D +IAIDALA+RS+ER+N+TIQI+D GIHPGSG+GNKR+ L+KE LGIP IAIGVPTV Sbjct: 184 DFVIAIDALAARSIERVNSTIQISDTGIHPGSGVGNKRKELSKETLGIPVIAIGVPTVVD 243 Query: 249 ASTIVNNVLEMMSRHFGQ--------------AGAGATQTKEI--------------MGM 280 A +I ++ ++ + +HFG+ AG + K++ +G Sbjct: 244 AVSITSDTIDFILKHFGREMKEGNKPSRSLLPAGFTFGEKKKLTEEDMPDEKSRNMFLGA 303 Query: 281 LDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNVG 340 + + + E+ L+ EVL PLGH+L+VTPKE+D FIE++AN++ASGLNAALH +D N G Sbjct: 304 VGTLGDEEKRKLIYEVLSPLGHNLMVTPKEVDAFIEDMANVIASGLNAALHHQIDQDNTG 363 Query: 341 AYTH 344 AYTH Sbjct: 364 AYTH 367 >ref|NP_834031.1| Spore protease [Bacillus cereus ATCC 14579] sp|Q818E2|GPR_BACCR Germination protease precursor (Spore protease) (GPR endopeptidase) (Germination proteinase) gb|AAP11232.1| Spore protease [Bacillus cereus ATCC 14579] Length = 367 Score = 362 bits (930), Expect = 5e-99, Method: Compositional matrix adjust. Identities = 183/364 (50%), Positives = 256/364 (70%), Gaps = 31/364 (8%) Query: 11 LDLQLYSVRTDLALEAKEMAQGPQKTP--IPGVNEEVEEADGIKVTRLAVANDAGSQAIG 68 LDL YS+RTDLA+EA +M Q Q+ I GV + E +G +T++ + ++A S+A+G Sbjct: 5 LDLSKYSIRTDLAVEAHQMLQESQEEQKGIQGVIVKEREEEGTIITKVTI-DEAASEAMG 63 Query: 69 RAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVTP 128 + GNY+TLEV +R DT LQQKV +FA+EF F++ +G+ + AS LIVGLGNWNVTP Sbjct: 64 KKPGNYLTLEVQGIRQQDTELQQKVERIFAKEFSCFLEEVGVTKEASCLIVGLGNWNVTP 123 Query: 129 DSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTKP 188 D+LGP+VVEN L+TR ++L P+ V G+R VSAI PGV+G+TGIE+S+V+ GII++T P Sbjct: 124 DALGPIVVENVLVTRHLFQLQPESVEEGFRPVSAIRPGVMGITGIETSDVIYGIIEKTNP 183 Query: 189 DVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVCY 248 D +IAIDALA+RS+ER+N+TIQI+D GIHPGSG+GNKR+ L+K+ LGIP IAIGVPTV Sbjct: 184 DFVIAIDALAARSIERVNSTIQISDTGIHPGSGVGNKRKELSKDTLGIPVIAIGVPTVVD 243 Query: 249 ASTIVNNVLEMMSRHFGQ--------------AGAGATQTKEI--------------MGM 280 A +I ++ ++ + +HFG+ AG + K++ +G+ Sbjct: 244 AVSITSDTIDFILKHFGREMKEGNKPSRSLLPAGFSFGEKKQLTQEDMPDEKSRNMFLGV 303 Query: 281 LDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNVG 340 + + E E+ L+ EVL LGH+L+V PKE+D FIE++AN++ASGLNAALH +D N G Sbjct: 304 VGTLEEEEKRRLIYEVLSLLGHNLMVPPKEVDSFIEDMANVIASGLNAALHHQIDQDNTG 363 Query: 341 AYTH 344 AYTH Sbjct: 364 AYTH 367 >ref|NP_242206.1| spore protease (degradation of SASPs) [Bacillus halodurans] sp|Q9KD78|GPR_BACHD Germination protease precursor (Spore protease) (GPR endopeptidase) (Germination proteinase) pir||D83817 SASP degradation spore proteinase (EC 3.4.-.-) gpr [similarity] - Bacillus halodurans (strain C-125) dbj|BAB05059.1| spore protease (degradation of SASPs) [Bacillus halodurans] Length = 372 Score = 360 bits (925), Expect = 2e-98, Method: Compositional matrix adjust. Identities = 182/374 (48%), Positives = 261/374 (69%), Gaps = 38/374 (10%) Query: 7 LIMELDLQLYSVRTDLALEAKEM--------AQGPQKTPIPGVNEEVEEADGIKVTRLAV 58 ++ EL+L+ Y+VRTDLA+EA ++ A+ P T + G + E DG+KV+++ V Sbjct: 1 MVKELNLEQYNVRTDLAIEAHDIVKEQEAQEAKQPAST-VEGFDIEETVMDGVKVSKV-V 58 Query: 59 ANDAGSQAIGRAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLI 118 + G++ G+ G Y+T E +R DT +Q+K+ VFA++F QFM +GI ++ + L+ Sbjct: 59 IHPLGAERTGKKAGRYLTFESQGIRKKDTDIQEKMERVFAQQFSQFMGELGITKDHTCLV 118 Query: 119 VGLGNWNVTPDSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEV 178 VGLGNWNVTPD+LGP+ VEN L+TR + L P++V G+R VSAIAPGV+G+TGIE+S+V Sbjct: 119 VGLGNWNVTPDALGPITVENLLVTRHLFTLAPEEVGEGFRPVSAIAPGVMGVTGIETSDV 178 Query: 179 VQGIIDRTKPDVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPC 238 + GII++TKPD +IA+DALASRS+ER+N TIQ++D GIHPGSG+GNKR+ L+KE LGIP Sbjct: 179 IYGIIEQTKPDFVIAVDALASRSIERVNATIQVSDTGIHPGSGVGNKRKELSKETLGIPV 238 Query: 239 IAIGVPTVCYASTIVNNVLEMMSRHFGQ------------AGAGAT-------------- 272 IA+G+PTV A TI ++ ++ + +HFG+ A AG T Sbjct: 239 IAVGIPTVVDAVTITSDAIDYVLKHFGRELREREKPSRALAPAGMTFGERRELTDEDLPP 298 Query: 273 --QTKEIMGMLDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAAL 330 + K +GM+ + E E+ L++EVL PLGH+L+VTPKE+D FIE++AN++ASGLNAAL Sbjct: 299 EEKRKTFLGMMGTLPEGEKRQLIQEVLAPLGHNLMVTPKEVDVFIEDMANVIASGLNAAL 358 Query: 331 HEAVDPGNVGAYTH 344 H+ V+ NVGAYTH Sbjct: 359 HQGVNQDNVGAYTH 372 >ref|NP_658353.1| Ca_channel_B, Dihydropyridine sensitive L-type calcium channel (Beta subunit) [Bacillus anthracis A2012] ref|NP_846769.1| spore protease [Bacillus anthracis str. Ames] sp|Q81LR5|GPR_BACAA Germination protease precursor (Spore protease) (GPR endopeptidase) (Germination proteinase) gb|AAP28255.1| spore protease [Bacillus anthracis str. Ames] Length = 368 Score = 358 bits (920), Expect = 8e-98, Method: Compositional matrix adjust. Identities = 185/365 (50%), Positives = 257/365 (70%), Gaps = 32/365 (8%) Query: 11 LDLQLYSVRTDLALEAKEMAQGPQKTPIP---GVNEEVEEADGIKVTRLAVANDAGSQAI 67 LDL YSVRTDLA+EA +M Q Q+ GV + E +GI +T++ + ++ S+++ Sbjct: 5 LDLSKYSVRTDLAVEAHQMLQERQEEQQQGIQGVIVKEREEEGIIITKVTI-DEVASESM 63 Query: 68 GRAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNVT 127 G+ GNY+TLEV +R DT LQQKV +FA+EF F++ +G+ + AS LIVGLGNWNVT Sbjct: 64 GKKPGNYLTLEVQGIRQQDTELQQKVERIFAKEFSYFLEEVGVTKEASCLIVGLGNWNVT 123 Query: 128 PDSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRTK 187 PD+LGP+VVEN L+TR ++L P+ V G+R VSAI PGV+G+TGIE+S+V+ GII++TK Sbjct: 124 PDALGPIVVENVLVTRHLFQLQPESVEEGFRPVSAIRPGVMGITGIETSDVIYGIIEKTK 183 Query: 188 PDVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTVC 247 PD +IAIDALA+RS+ER+N+TIQI+D GIHPGSG+GNKR+ L+KE LGIP IAIGVPTV Sbjct: 184 PDFVIAIDALAARSIERVNSTIQISDTGIHPGSGVGNKRKELSKETLGIPVIAIGVPTVV 243 Query: 248 YASTIVNNVLEMMSRHFGQ--------------AGAGATQTKEI--------------MG 279 A +I ++ ++ + +HFG+ AG + K++ +G Sbjct: 244 DAVSITSDTIDFILKHFGREMKEGNKPSRSLLPAGFTFGEKKKLTEEDMPDEKSRNMFLG 303 Query: 280 MLDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNV 339 + + + E+ L+ EVL PLGH+L+VTPKE+D FIE++AN++ASGLNAALH +D N Sbjct: 304 AVGTLEDEEKRKLIYEVLSPLGHNLMVTPKEVDAFIEDMANVIASGLNAALHHQIDQDNT 363 Query: 340 GAYTH 344 GAYTH Sbjct: 364 GAYTH 368 >ref|NP_390432.1| spore protease [Bacillus subtilis] sp|P22322|GPR_BACSU Germination protease precursor (Spore protease) (GPR endopeptidase) (Germination proteinase) pir||B39198 SASP degradation spore proteinase (EC 3.4.-.-) gpr precursor - Bacillus subtilis gb|AAA22500.1| spore protease dbj|BAA12457.1| Gpr [Bacillus subtilis] emb|CAB14496.1| spore protease [Bacillus subtilis subsp. subtilis str. 168] Length = 368 Score = 351 bits (901), Expect = 1e-95, Method: Compositional matrix adjust. Identities = 175/365 (47%), Positives = 248/365 (67%), Gaps = 31/365 (8%) Query: 10 ELDLQLYSVRTDLALEAKEMAQGPQKTP---IPGVNEEVEEADGIKVTRLAVANDAGSQA 66 ELD+ Y +RTDLA+E KE Q P I G E+ + GIK+ + V + G++ Sbjct: 5 ELDVNQYLIRTDLAVETKEAMANQQAVPTKEIKGFIEKERDHGGIKIRTVDVTKE-GAEL 63 Query: 67 IGRAIGNYVTLEVPALRGGDTGLQQKVSVVFAREFEQFMDRIGIGRNASVLIVGLGNWNV 126 G+ G Y+TLE +R D+ +Q+KVS VFA EF F++ + I ++AS LIVGLGNWNV Sbjct: 64 SGKKEGRYLTLEAQGIRENDSEMQEKVSAVFAEEFSAFLENLNISKDASCLIVGLGNWNV 123 Query: 127 TPDSLGPLVVENSLITRQFYELVPDQVSPGYRNVSAIAPGVLGLTGIESSEVVQGIIDRT 186 TPD+LGP+ VEN L+TR ++L P+ V GYR VSA APGV+G+TGIE+S++++G+I+++ Sbjct: 124 TPDALGPMAVENLLVTRHLFKLQPENVQEGYRPVSAFAPGVMGITGIETSDIIKGVIEQS 183 Query: 187 KPDVIIAIDALASRSLERINTTIQIADIGIHPGSGIGNKRRGLTKEILGIPCIAIGVPTV 246 KPD +IAIDALA+R++ER+NTTIQI+D GIHPGSG+GNKR+ L+K+ LG+P IAIGVPTV Sbjct: 184 KPDFVIAIDALAARAVERVNTTIQISDTGIHPGSGVGNKRKDLSKDTLGVPVIAIGVPTV 243 Query: 247 CYASTIVNNVLEMMSRHFGQ-----------AGAGAT----------------QTKEIMG 279 A TI ++ ++ + +HFG+ AG T Q + +G Sbjct: 244 VDAVTIASDTVDYILKHFGREMKDNRPSRSLVPAGMTFGKKKVLTEDDLPDQKQRQSFLG 303 Query: 280 MLDDISEHERLSLVKEVLEPLGHDLIVTPKEIDEFIEEIANIVASGLNAALHEAVDPGNV 339 ++ + E E+ L+ EVL PLGH+L+VTPKE+D FI+++AN++A+GLN ALHE V N Sbjct: 304 IVGTLQEDEKRQLIHEVLSPLGHNLMVTPKEVDSFIDDMANVLANGLNTALHEKVSQENK 363 Query: 340 GAYTH 344 G+Y H Sbjct: 364 GSYNH 368 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.316 0.136 0.379 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 484,793,814 Number of extensions: 20006835 Number of successful extensions: 55020 Number of sequences better than 1.0e-05: 14 Number of HSP's gapped: 54996 Number of HSP's successfully gapped: 14 Length of query: 344 Length of database: 757,237,578 Length adjustment: 126 Effective length of query: 218 Effective length of database: 415,135,104 Effective search space: 90499452672 Effective search space used: 90499452672 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 126 (53.1 bits)