BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0024.p (282 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value emb|CAD69017.1| ComER protein [Bacillus megaterium] 207 3e-52 ref|NP_242197.1| non-essential gene for competence (pyrroline-5-... 201 2e-50 ref|NP_980702.1| pyrroline-5-carboxylate reductase, putative [Ba... 196 7e-49 ref|NP_834037.1| ComE operon protein 4 [Bacillus cereus ATCC 145... 195 1e-48 ref|NP_658359.1| P5CR, Delta 1-pyrroline-5-carboxylate reductase... 193 4e-48 >emb|CAD69017.1| ComER protein [Bacillus megaterium] Length = 276 Score = 207 bits (526), Expect = 3e-52, Method: Compositional matrix adjust. Identities = 106/265 (40%), Positives = 156/265 (58%), Gaps = 1/265 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIG-SNQETA 59 M +GFIGTG+MG +LIDAF+ S A+ P + NR+ R ++QQ+H I++ + +E Sbjct: 1 MNIGFIGTGNMGKILIDAFIESKAVRPSKITIMNRTKKRAKEIQQQHKKINVADTAEEVV 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 S ++FICVKPL+ L ++ L + +VSITSP+ + +E +PS+ A++IPSIT Sbjct: 61 HASKVIFICVKPLDIHPLLIKLEPLLTKDHCIVSITSPISVEQIERLIPSQAARIIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R +G SL FG R + L L S M P+ I + TR++SD SCGPAF SF L Sbjct: 121 NRALAGVSLFTFGERCTPFYQNYLHELFSSMSKPVAIDQTITRVSSDIVSCGPAFFSFLL 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 +R+I+AAV+ T I EE T +A F+ LQ++V V GG+T E + Sbjct: 181 QRFIDAAVQETNISEEEATVMASDMIIGMGKLLEKEIFTLPTLQDKVCVKGGVTGEGIKV 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVE 264 L L +F + TH+K+DED+E Sbjct: 241 LEAELGEMFVHVFQKTHEKFDEDIE 265 >ref|NP_242197.1| non-essential gene for competence (pyrroline-5-carboxylate reductase) [Bacillus halodurans] pir||C83816 non-essential gene for competence (pyrroline-5-carboxylate reductase) comER [imported] - Bacillus halodurans (strain C-125) dbj|BAB05050.1| non-essential gene for competence (pyrroline-5-carboxylate reductase) [Bacillus halodurans] Length = 275 Score = 201 bits (511), Expect = 2e-50, Method: Compositional matrix adjust. Identities = 107/269 (39%), Positives = 162/269 (60%), Gaps = 1/269 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSN-QETA 59 MK+GFIGTGSMG++LI++FL S AL ++ +NR+ + L +R+PG+S+ +E Sbjct: 1 MKIGFIGTGSMGTILIESFLESSALTAEQIVITNRTQEKADALARRYPGLSVVDRPEEVV 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 Q +D F+CVKP++F + +I + +++VSITSP+ + LE+++ K+A+ IPSI Sbjct: 61 QQTDCFFVCVKPMQFHRVLMDIRPHCSANKLLVSITSPISIAQLENSVDCKVARAIPSIL 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R +G++L F R +DR L LL + P+ I+E TR++SD SCGPAF S+ Sbjct: 121 NRACAGSTLVSFSERCLEEDRSFLCGLLGTISEPLLIEEKITRVSSDIVSCGPAFFSYLT 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 +R+IEAAV T I EE T LA ++ LQ+RV VPGGIT E + Sbjct: 181 QRFIEAAVRQTAISEEDATALATEMLIGIGKLLDQELYTLPSLQKRVCVPGGITGEGIRV 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDA 268 L + +F+RL TH KY + EK+ + Sbjct: 241 LEEDVGHMFDRLFQKTHQKYYHEQEKISS 269 >ref|NP_980702.1| pyrroline-5-carboxylate reductase, putative [Bacillus cereus ATCC 10987] gb|AAS43310.1| pyrroline-5-carboxylate reductase, putative [Bacillus cereus ATCC 10987] Length = 275 Score = 196 bits (497), Expect = 7e-49, Method: Compositional matrix adjust. Identities = 101/273 (36%), Positives = 161/273 (58%), Gaps = 1/273 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSN-QETA 59 M +G IGTG+MG++LIDAFL + A++P + NR+P + +++++P + I E Sbjct: 1 MNIGIIGTGNMGNILIDAFLETRAVKPSCLTIINRTPAKAYHIKEKYPSVHIAKTIDEAI 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 + S ++FICVKP++ + + + E+ +VSITSP+ LE+ +P +A++IPSIT Sbjct: 61 ERSHLIFICVKPIDIYPILQKYAEHFSDEKCLVSITSPISPSQLETLVPCHVARIIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R SG SL FGS + + + L RL + P+ I+E TR++SD +SCGPAF S+ L Sbjct: 121 NRALSGASLFTFGSNCSEEWQQKLFRLFKNISTPLVIEEDITRVSSDIASCGPAFFSYLL 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 + +I AAV+ T I E T L F+ LQE+V V GG+T E + Sbjct: 181 QCFINAAVDKTNITHEEATTLVSEMVVGMGKLLEKGIFTLPTLQEKVCVKGGVTGEGIRV 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDALYGR 272 L + +F +LI TH+K+DED++ +D + + Sbjct: 241 LEEHVGDMFHKLIERTHEKFDEDLKCVDQQFNK 273 >ref|NP_834037.1| ComE operon protein 4 [Bacillus cereus ATCC 14579] gb|AAP11238.1| ComE operon protein 4 [Bacillus cereus ATCC 14579] Length = 275 Score = 195 bits (496), Expect = 1e-48, Method: Compositional matrix adjust. Identities = 101/273 (36%), Positives = 159/273 (58%), Gaps = 1/273 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSN-QETA 59 M +G IGTG+MG++LIDAFL + A++P + NR+P + +++++P + I E Sbjct: 1 MNIGIIGTGNMGNILIDAFLETRAVKPSCLTIINRTPAKAYHIKEKYPSVHIAKTIPEVI 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 + S ++FICVKP++ + + + E +VSITSP+ LE+ +P +A++IPSIT Sbjct: 61 EQSQLIFICVKPIDIYPILKKYAEHFSDETCLVSITSPISPSQLETLVPCHVARIIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R SG SL FG + + + L RL + P+ I+E TR++SD +SCGPAF S+ L Sbjct: 121 NRALSGASLFTFGDNCSEEWQQKLLRLFKNISTPLVIEEDITRVSSDIASCGPAFFSYLL 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 + +I AAV+ T I E T L F+ LQE+V V GG+T E + Sbjct: 181 QCFINAAVDKTNITHEEATTLVSEMVVGMGKLLEKEIFTLPTLQEKVCVKGGVTGEGIRV 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDALYGR 272 L + +F +LI TH+K+DED+E +D + + Sbjct: 241 LEEHVGDMFHKLIERTHEKFDEDLECVDQQFNK 273 >ref|NP_658359.1| P5CR, Delta 1-pyrroline-5-carboxylate reductase [Bacillus anthracis A2012] Length = 275 Score = 193 bits (490), Expect = 4e-48, Method: Compositional matrix adjust. Identities = 99/273 (36%), Positives = 161/273 (58%), Gaps = 1/273 (0%) Query: 1 MKVGFIGTGSMGSLLIDAFLSSGALEPCDVLASNRSPNRLLQLQQRHPGISIGSN-QETA 59 M +G IGTG+MG++LIDAFL + A++P + NR+P + +++++P + I + Sbjct: 1 MNIGIIGTGNMGNILIDAFLETRAVKPSCLTIINRTPAKAYHIKEKYPSVHIAKTIDKVI 60 Query: 60 QGSDIVFICVKPLEFKTLTDEISSSLRSEQIVVSITSPVMLHHLESALPSKIAKVIPSIT 119 + S ++FICVKP++ + + + E+ +VSITSP+ LE+ +P +A++IPSIT Sbjct: 61 ERSHLIFICVKPIDIYPILQKYAEHFSDEKCLVSITSPISPSQLETLVPCHVARIIPSIT 120 Query: 120 HRVKSGTSLCIFGSRLNTKDRLLLQRLLSFMGVPIEIQEMHTRIASDFSSCGPAFISFFL 179 +R SG SL FG+ + + + L RL + P+ I+E TR++SD +SCGPAF S+ L Sbjct: 121 NRALSGASLFTFGNNCSEEWQQKLFRLFKNISTPLVIKEDITRVSSDIASCGPAFFSYLL 180 Query: 180 ERWIEAAVEATGIDEELITRLAXXXXXXXXXXXXXXXFSPQELQERVAVPGGITAEALSH 239 + +I AAV+ T I E T L F+ LQE+V V GG+T E + Sbjct: 181 QCFINAAVDKTNITHEEATTLVSEMVVGMGELLEKGIFTLPTLQEKVCVKGGVTGEGIRV 240 Query: 240 LRTSLEGVFERLILTTHDKYDEDVEKLDALYGR 272 L + +F +LI TH+K+DED++ +D + + Sbjct: 241 LEEHVGDMFHKLIERTHEKFDEDLKCVDQQFNK 273 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.320 0.136 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 361,658,784 Number of extensions: 12799836 Number of successful extensions: 33758 Number of sequences better than 1.0e-05: 262 Number of HSP's gapped: 33535 Number of HSP's successfully gapped: 262 Length of query: 282 Length of database: 757,237,578 Length adjustment: 124 Effective length of query: 158 Effective length of database: 420,565,302 Effective search space: 66449317716 Effective search space used: 66449317716 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 125 (52.8 bits)