BLASTP 2.2.18 [Mar-02-2008] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Query= 0290.0014.p (581 letters) Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF 2,715,099 sequences; 757,237,578 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|NP_782429.1| two-component sensor kinase yesM [Clostridium t... 154 8e-36 ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongen... 149 2e-34 ref|ZP_00100011.1| COG2972: Predicted signal transduction protei... 142 2e-32 ref|NP_665005.1| putative two-component sensor histidine kinase ... 140 7e-32 ref|NP_607635.1| putative histidine kinase [Streptococcus pyogen... 139 2e-31 >ref|NP_782429.1| two-component sensor kinase yesM [Clostridium tetani E88] gb|AAO36366.1| two-component sensor kinase yesM [Clostridium tetani E88] Length = 577 Score = 154 bits (388), Expect = 8e-36, Method: Compositional matrix adjust. Identities = 121/440 (27%), Positives = 198/440 (45%), Gaps = 30/440 (6%) Query: 154 KGSAEAGFRLLLSDPIESRNDPRQAGLLMSMYFPVSN--------IERMFDAYQSQNKGD 205 K + ++S P+ SR+D + L+ +P+ N I + + N Sbjct: 132 KKAMSTNVEYVISKPLVSRSDNKPIFLIC---YPILNDNKDKIGFINGSVNLDKISNIIH 188 Query: 206 TFLFHPAFGVILSRNTD-----------RELAERIVATLTSSTGQGSSDIFSLK-----E 249 H F I+++NTD ++ ++ + + + SS +LK + Sbjct: 189 DIDIHNGFAWIMNKNTDIYSTTKENLNSNYISLEELSCIANQSKNKSSGTVALKNVHNKD 248 Query: 250 GSFLVSSVPSSAINWQVVHYSPLKQILQPIRSSRYSFLTGXXXXXXXXXXXXXXXYRQVQ 309 + SSVP + NW + K+I I L + Sbjct: 249 STVFFSSVPYTE-NWILCIIVENKKIHTQINQIINLILFVGLILLLAAILLSIVISGSIV 307 Query: 310 RPVSLLLRSLNRMKEGRWSTRIHTRTNPEFTMLNEEFNEMAEKIQSLIEQVYLEQLRAKD 369 +P+ L ++ + +G + + + N E ++L + FN+M I+ LI +VY + + + Sbjct: 308 KPIHRLKSNMLEISKGNFDSYYEIKNNDEISILGQVFNQMLTDIKRLINKVYQVEKQKRS 367 Query: 370 AHLKQLQSQINPHFLYNCLFFIKSKAVVGDTGSVEAMALNLGEYYRYITKADHSLTTLQD 429 A L+ LQSQINPHFLYN L I+ KA+ + V M L ++R T+ D Sbjct: 368 AELRVLQSQINPHFLYNTLDTIQWKALEYNAFEVVDMINYLSNFFRISLSDGKEFITISD 427 Query: 430 ELKLLENYLEIQNLR-KQRICYEMDIADDLLGLHIPRLLIQPLVENSIIHGIEKKIGQGF 488 E+ + NYLEIQ +R K +I Y MD+ L IP+L++QPLVENSI HG++ + +G Sbjct: 428 EVAHVSNYLEIQKIRYKDKISYSMDVDSSLDQYLIPKLIVQPLVENSIYHGLKLQKHKGI 487 Query: 489 IRITASXXXXXXXXXXXDNGAGMTSEAIASLHARMSEPTREDGGCGLWNIQQRLKTHYGD 548 I ++ S DNG GM E + +L + E E GL+NI +RLK +G Sbjct: 488 INVSISSKDGFLFIKVIDNGLGMDVEQLRTLRKNLWESI-ETNHYGLYNINERLKLAFGK 546 Query: 549 SSGLLIAPSSEGGLQITLRM 568 + I + G Q+ LR+ Sbjct: 547 KYSIEIKSELKIGTQVLLRI 566 >ref|NP_623881.1| predicted ATPase [Thermoanaerobacter tengcongensis] gb|AAM25485.1| predicted ATPase [Thermoanaerobacter tengcongensis] Length = 593 Score = 149 bits (376), Expect = 2e-34, Method: Compositional matrix adjust. Identities = 92/328 (28%), Positives = 157/328 (47%), Gaps = 5/328 (1%) Query: 244 IFSLKEGSFLVSSVPSSAINWQVVHYSPLKQILQPIRSSRYSFLTGXXXXXXXXXXXXXX 303 + ++ LV+S P +W++V LK + IR S F Sbjct: 259 VININSSKLLVTSSPIPTSDWRLVSVVSLKNAINSIRISFIEFYLRILVFCLLFAFLVSM 318 Query: 304 XY-RQVQRPVSLLLRSLNRMKEGRWSTRIHTR--TNPEFTMLNEEFNEMAEKIQSLIEQV 360 + R + P+ L S+ ++++G ++ + E ++ +E+N M E+I LI ++ Sbjct: 319 YFSRALTHPLERLANSMKKVRQGNLEEKVEFEYHSKDEIAIVIKEYNRMVERINDLINRI 378 Query: 361 YLEQLRAKDAHLKQLQSQINPHFLYNCLFFIKSKAVVGDTGSVEAMALNLGEYYRYITKA 420 ++ ++A LK L++QINPHFLYN L I KA + M +L +R Sbjct: 379 LKLEINKREAELKALEAQINPHFLYNTLDTIFWKAEKAKDRELSEMIYSLSRLFRLTLNR 438 Query: 421 DHSLTTLQDELKLLENYLEIQNLR-KQRICYEMDIADDLLGLHIPRLLIQPLVENSIIHG 479 ++ E +L+E YL +Q+ R K + Y + I+D++L +IP+L++QP VEN+I+HG Sbjct: 439 GEEFIQVRGEKELIERYLFLQSKRYKNKFKYSVKISDEILDYYIPKLILQPFVENAIVHG 498 Query: 480 IEKKIGQGFIRITASXXXXXXXXXXXDNGAGMTSEAIASLHARM-SEPTREDGGCGLWNI 538 +E + F+ + DNG GM+ E + L + SE E G + N+ Sbjct: 499 LENNSEESFVEVIGEKEGENLKFIIKDNGPGMSEETLEKLKRFIESENIEEKVGYAIRNV 558 Query: 539 QQRLKTHYGDSSGLLIAPSSEGGLQITL 566 +RL+ +YGD L I G I+L Sbjct: 559 NERLRLYYGDKYELKIESRQGEGTTISL 586 >ref|ZP_00100011.1| COG2972: Predicted signal transduction protein with a C-terminal ATPase domain [Desulfitobacterium hafniense] Length = 577 Score = 142 bits (359), Expect = 2e-32, Method: Compositional matrix adjust. Identities = 89/264 (33%), Positives = 141/264 (53%), Gaps = 9/264 (3%) Query: 308 VQRPVSLLLRSLNRMKEGRWSTRIHTRTNPEFTMLNEEFNEMAEKIQSLIEQVYLEQLRA 367 V RP+ L +++ +++G +T + + E L+ F M ++I+ L++++ ++ Sbjct: 315 VARPIRQLEKAMEEVEKGSLATEVGVVGSAEIASLSTHFLSMLDRIRELMQEIRNKEQFL 374 Query: 368 KDAHLKQLQSQINPHFLYNCLFFIKSKAVVGDTGSVEAMALNLGEYYRYITKADHSLTTL 427 + + LK L SQINPHFLYN L I A G+T V ++ + ++R + TT+ Sbjct: 375 RASELKTLYSQINPHFLYNTLDTIVWLAEFGNTERVITVSKAMARFFRLSLRGGSETTTV 434 Query: 428 QDELKLLENYLEIQNLRKQ-RICYEMDIADDLLGLHIPRLLIQPLVENSIIHGIEKKIGQ 486 +DEL + YL IQ R Q ++ YE+ D L+ + IP++L+QPLVEN+I HG+ K G Sbjct: 435 RDELDHVRQYLFIQKERYQDKLSYEIQAEDGLMDITIPKILLQPLVENAIYHGLRKMPGG 494 Query: 487 GFIRITASX-XXXXXXXXXXDNGAGMTSEAIASLHARMSEPTREDGGCGLWNIQQRLKTH 545 G IRITA DNG G + +P R GG GL N+ +R++ + Sbjct: 495 GMIRITARKGEKEDLFLVVEDNGGGFED------AEKGKDPLRL-GGVGLKNVAERIRLY 547 Query: 546 YGDSSGLLIAPSSEGGLQITLRME 569 YG+ GL IA G I LR++ Sbjct: 548 YGEDYGLSIANRPGQGATIILRLK 571 >ref|NP_665005.1| putative two-component sensor histidine kinase [Streptococcus pyogenes MGAS315] ref|NP_801923.1| putative two-component sensor histidine kinase [Streptococcus pyogenes SSI-1] gb|AAM79808.1| putative two-component sensor histidine kinase [Streptococcus pyogenes MGAS315] dbj|BAC63756.1| putative two-component sensor histidine kinase [Streptococcus pyogenes SSI-1] Length = 577 Score = 140 bits (354), Expect = 7e-32, Method: Compositional matrix adjust. Identities = 109/380 (28%), Positives = 178/380 (46%), Gaps = 19/380 (5%) Query: 202 NKGDTFLFHPAFGVILSRNTDRELAERIVATLTSSTGQGSSDIFSLKEGSFLVSS-VPSS 260 N F++HP V ++ +E+A + L G ++ + SF+ +P+S Sbjct: 211 NDKHEFVYHPKKSVY---SSSKEMAA-MKPYLAIQNG------YTKDKTSFVYQKLIPNS 260 Query: 261 AINWQVVHYSPLKQILQPIRSSRYSFLTGXXXXXXXXXXXXXXXYRQVQRPVSLLLRSLN 320 W +V + L Q+ + R +SFL R+ RP+ L + + Sbjct: 261 --QWTLVGVASLDQLHRVQRQILWSFLGTGLLACLICGFATVLALRRWIRPIQQLQQVIL 318 Query: 321 RMKEGRWSTRIHTRTNPEFTMLNEEFNEMAEKIQSLIEQVYLEQLRAKDAHLKQLQSQIN 380 +++G R +PE T L ++FN + ++I SL+ V ++ L+ L SQIN Sbjct: 319 AIQKGDRQLRAQETGSPELTDLAQQFNALLDQIDSLMVAVADKEKAIGQYRLQALASQIN 378 Query: 381 PHFLYNCLFFIKSKAVVGDTGSVEAMALNLGEYYRYITKADHSLTTLQDELKLLENYLEI 440 PHFLYN L I A D+ V + +L +Y+R + L DEL + YL I Sbjct: 379 PHFLYNTLDTIIWMAEFNDSKRVVEVTKSLAKYFRLALNQGNEYIRLADELDHVSQYLFI 438 Query: 441 QNLR-KQRICYEMDIADDLLGLHIPRLLIQPLVENSIIHGIEKKIGQGFIRITASXXXXX 499 Q R ++ YE+ D IP+L++QPLVEN+I HGI++ +G I++T S Sbjct: 439 QKQRYGDKLSYEVQGLDVYADFVIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSDTAQH 498 Query: 500 XXXXXXDNGAGMTSEAIASLHARMSEPTREDGGCGLWNIQQRLKTHYGDSSGLLIAPSSE 559 DNG G+ ++ + + ++ GG GL N+ QRLK HYG+ + I S+ Sbjct: 499 LMLTVWDNGKGIEDSSLTNSQSLLAR-----GGVGLKNVDQRLKLHYGEGYHMTIHSQSD 553 Query: 560 GGLQITLRMEKKEESYAPNS 579 +I L + K E A ++ Sbjct: 554 QFTEIQLSLPKMHELMADDT 573 >ref|NP_607635.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] gb|AAL98134.1| putative histidine kinase [Streptococcus pyogenes MGAS8232] Length = 577 Score = 139 bits (350), Expect = 2e-31, Method: Compositional matrix adjust. Identities = 108/380 (28%), Positives = 178/380 (46%), Gaps = 19/380 (5%) Query: 202 NKGDTFLFHPAFGVILSRNTDRELAERIVATLTSSTGQGSSDIFSLKEGSFLVSSV-PSS 260 N F++HP V ++ +E+A + L G ++ + SF+ V P+S Sbjct: 211 NDKHEFVYHPKKSVY---SSSKEMAA-MKPYLAIKNG------YAKDKSSFVYQRVIPNS 260 Query: 261 AINWQVVHYSPLKQILQPIRSSRYSFLTGXXXXXXXXXXXXXXXYRQVQRPVSLLLRSLN 320 W ++ + L Q+ + R +SFL R+ RP+ L + + Sbjct: 261 --QWTLIGVASLDQLHRVQRQIFWSFLGMGLLACLICGLTTILVLRKWIRPIQQLQQVIL 318 Query: 321 RMKEGRWSTRIHTRTNPEFTMLNEEFNEMAEKIQSLIEQVYLEQLRAKDAHLKQLQSQIN 380 +++G R +PE T L +FN + ++I SL+ V ++ L+ L SQIN Sbjct: 319 AIQKGNRQLRAQETGSPELTDLAHQFNALLDQIDSLMVAVADKEKEIGQYRLQALASQIN 378 Query: 381 PHFLYNCLFFIKSKAVVGDTGSVEAMALNLGEYYRYITKADHSLTTLQDELKLLENYLEI 440 PHFLYN L I A D+ V + +L +Y+R + L DEL + YL I Sbjct: 379 PHFLYNTLDTIIWMAEFNDSKRVVEVTKSLAKYFRLALNQGNDYIRLADELDHVSQYLFI 438 Query: 441 QNLR-KQRICYEMDIADDLLGLHIPRLLIQPLVENSIIHGIEKKIGQGFIRITASXXXXX 499 Q R ++ Y++ D IP+L++QPLVEN+I HGI++ +G I++T S Sbjct: 439 QKQRYGDKLTYDVQGLDAYADFIIPKLILQPLVENAIYHGIKEVDRKGMIKVTVSETAQH 498 Query: 500 XXXXXXDNGAGMTSEAIASLHARMSEPTREDGGCGLWNIQQRLKTHYGDSSGLLIAPSSE 559 DNG G+ + A+ + + ++ GG GL N+ QRLK YG++ + I S+ Sbjct: 499 LILTVWDNGKGIEASALTNSQSLLTR-----GGVGLKNVDQRLKLQYGEAYQMTIHSQSD 553 Query: 560 GGLQITLRMEKKEESYAPNS 579 +I L + K E A ++ Sbjct: 554 HFTEIQLSLPKMHELMADDT 573 Database: All non-redundant GenBank CDS translations+PDB+SwissProt+PIR+PRF Posted date: Mar 24, 2004 1:53 AM Number of letters in database: 757,237,578 Number of sequences in database: 2,715,099 Lambda K H 0.318 0.134 0.384 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 2715099 Number of Hits to DB: 760,954,669 Number of extensions: 28788871 Number of successful extensions: 75087 Number of sequences better than 1.0e-05: 172 Number of HSP's gapped: 74787 Number of HSP's successfully gapped: 172 Length of query: 581 Length of database: 757,237,578 Length adjustment: 131 Effective length of query: 450 Effective length of database: 401,559,609 Effective search space: 180701824050 Effective search space used: 180701824050 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 129 (54.3 bits)